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Study of the Extract Activities of Buas buas Leaves (Premna pubescens) as Immunostimulant on Rats (Rattus novegicus)

Received: 28 November 2014     Accepted: 14 December 2014     Published: 19 December 2014
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Abstract

Buas buas (Premna pubescens) is one of the plants in Indonesia which is believed to have medicinal properties, but it is not certain. This plant is consumed by the Malay community, one of the tribes in North Sumatra - Indonesia, as vegetables, especially during the fasting month. This paper describes the effects of ethanol extract of leaves of Buas buas as an immunostimulant in rats (Rattus norvegicus). The experiments were performed on 24 tail of male rats, which were three months old, weighing 140-180 g. There were four groups of experiment ie Group A0 was given Carboxy Methyl Cellulose mice (CMC) (control group), group A1 was given 500 mg/ kg bw of Ethanol Extracts of Buas buas. Group A2, given ethanol extracts of Buas buas 500 mg / kg bw + SRBC, and the group A3 only given Sheep Red Blood Cell (SRBC). After this experiment, apigenin levels were analyzed by High Performance Liquid Chromatography (HPLC) Agilent 1220. Then the activity of immunostimulation is determinated by measuring the leukocyte count, percentage of lymphocytes, antibody titers, the levels of immunoglobulin M (IgM), immunoglobulin G (IgG), and lysozyme with ABX Micros 60. The group A2 is seen the highest group of in the number of leukocytes, antibody titer levels, imonoglobulin G (IgG), and imonoglobulin M. The amount of lysozyme owned group A2 is 0:04 ug / ml, whereas other treatments to obtain results that are not different relative significantly. Provision of Ethanol Extracts of Buas buas on mice can increase the leukocytes, lymphocytes, antibody titers, IgM, IgG and Lysozyme.

Published in American Journal of BioScience (Volume 2, Issue 6)
DOI 10.11648/j.ajbio.20140206.19
Page(s) 244-250
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Premna Pubescens, Titer Antibodies, Lymphocytes, SRBC, IgG, IgM, Lysozyme

References
[1] Lie, T., et. all., 2010. Flavonoid, Phenol and Polysaccharide Contents of Echinacea purpurea L. and Its Immunostimulant Capacity In Vitro. International Journ. of Environmental Science and Development. Vol. 1 No. 1. http://dx.doi.org/10.7763/ijesd.2010.v1.2
[2] Thiruvenkatasubramaniam, R., Jayakar, B. 2010. Anti-hypreglycemic and anti-hyperlipidemic activities of Premna corymbosa (Burm.F) Rottl on Streptozotocin induced diabetic rats. Der Pharmacia Lettre. 2 (1) 505-509.
[3] Gates M.A, Vitonis, A.F, Tworoger S.S, Rosner, B, Ernstoff, L.T, Hankinson, S.E, dan Cramer D.W, 2009. Flavonoid intake and ovarium cancer risk in a Polution based case-control study. International Journal of Cancer. 124, 1918-1925. http://dx.doi.org/10.1002/ijc.24151.
[4] Chen, D., Piwowar, K.R.L., Chen, M.S., Dou, Q.P, 2007. Inhibition of Proteasome Activity by the Dietary Flavonoid Apigenin is Associated with Growth Inhibition in Cultured Breast Cancer Cells and Xe7nografts. Breast Cancer Research. 9. 1-8. http://dx.doi.org/10.1186/bcr1797.
[5] Fang, J., Xia, C., Cao. Z., Zheng, J.Z., Reed, E., dan Jiang, B.H. 2005. Apigenin inhibits VEGF and HIF-1 expression via P13K/AKT/ p7056K1 and HDM2/p53 pathways. The FASEB journal.19. 342-353. http://dx.doi.org/10.1096/fj.04-2175com
[6] Zheng. P.W, Chiang. L.C, Lin, C.C. 2005. Apigenin Induced Apoptosis through p53-dependent pathway in human Cervikal Carcinova Cells. Life Science. Volume 76. Issue 12. http://dx.doi.org/10.1016/j.lfs.2004.08.023
[7] Torkin R. Lavoie JF. Kaplan DR. Yeger H (2005). Induction of Caspase-Dependent, P-53 Mediated Apoptosis by Apigenin in Human Neuro blastoma. Molecolar Cancer Theurapetic. Vol 4. Edition 1. American Association for Cancer research.
[8] Bairwa, M.K., Jakhar, J.K., Y. Satyanarayana., Reddy, A.D, 2012. Animal and Plant Originated Immunostimulants Used in Aquaculture. Journal of Natural Product and Plant Resource. 2 (3): 397-400.
[9] Ogier de Baulny, M., Quentell, C., Vournier, V. Lamour, F and R. Guevello. 1996. Effect of long-term oral administration of β-glucan as an immunostimulant or an adjuvant on some non-specific parameters of the immune response of turbot Scophthalmus maximus. Diseases of aquatic organisms. Vol 26: 139 – 147. http://dx.doi.org/10.3354/dao026139
[10] Khumar, S., Gupta, P., Sharma S. and D Khumar. 2011. A Review on Immunostimulatory plant. Journal of Chinese Integrative Medicine. Vol. 9 No.2: 117-128. http://dx.doi.org/10.3736/jcim20110201.
[11] Jian, J dan Wu, Z. 2004. Influence of Traditional Chinese Medicine on Non-Specific Immunity of Jian Carp (Cyprinus carpio var. Jian). Fish and Shellfish Immunology. 16. 185-191. http://dx.doi.org/10.1016/s1050-4648(03)00062-7.
[12] Ellis, A. 1988. Fish faccination. Academic Press, San Diego, C.A. USA. 1-16
[13] Charles River Laboratories, 1998. Baseline Hematology and Clinical Chemistry Value for Charles River Wistar Rats-(CRL: (WI) BR) as a Function of Sex and Age. Technical Bulletin.
[14] Siahaan, P.M. 2010. Immunologi. FMIPA UNIMED. Medan.
[15] Farhath, S., Vijaya dan Vimal. 2013. Immunomodulatory activity of geranial acetate, gingerol, and eudenol essential oils: evidence for humoral and cell-mediated responses. Avicenna Journal of Phytomedicine. 1-7.
[16] Roitt, I. 2001. Roitt’s Essential Immunology, 8th ed.Blackwell Science.
[17] Kannan, M., Singh, A.R., Kumar, T.A., Jegatheswari, P., dan Subburayalu, S. 2007. Studies on Immuno-bioactivities of Nyctanthes arbortritis (Oleacea). African Journal of Microbiology Research, 1 (6). 088-091.
[18] Shiney, R.B, Ganesh P, and R. S. Khumar. 2012. Phytochemical Screening of Coleus aromaticus and Leucas aspera and Their Antibacterial Activity against Entheric Phatogens. International Journal of Pharmaceutical and Biological Archives. 3(1): 162-166.
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    Martina Restuati, Syafruddin Ilyas, Salomo Hutahaean, Herbert Sipahutar. (2014). Study of the Extract Activities of Buas buas Leaves (Premna pubescens) as Immunostimulant on Rats (Rattus novegicus). American Journal of BioScience, 2(6), 244-250. https://doi.org/10.11648/j.ajbio.20140206.19

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    ACS Style

    Martina Restuati; Syafruddin Ilyas; Salomo Hutahaean; Herbert Sipahutar. Study of the Extract Activities of Buas buas Leaves (Premna pubescens) as Immunostimulant on Rats (Rattus novegicus). Am. J. BioScience 2014, 2(6), 244-250. doi: 10.11648/j.ajbio.20140206.19

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    AMA Style

    Martina Restuati, Syafruddin Ilyas, Salomo Hutahaean, Herbert Sipahutar. Study of the Extract Activities of Buas buas Leaves (Premna pubescens) as Immunostimulant on Rats (Rattus novegicus). Am J BioScience. 2014;2(6):244-250. doi: 10.11648/j.ajbio.20140206.19

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  • @article{10.11648/j.ajbio.20140206.19,
      author = {Martina Restuati and Syafruddin Ilyas and Salomo Hutahaean and Herbert Sipahutar},
      title = {Study of the Extract Activities of Buas buas Leaves (Premna pubescens) as Immunostimulant on Rats (Rattus novegicus)},
      journal = {American Journal of BioScience},
      volume = {2},
      number = {6},
      pages = {244-250},
      doi = {10.11648/j.ajbio.20140206.19},
      url = {https://doi.org/10.11648/j.ajbio.20140206.19},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20140206.19},
      abstract = {Buas buas (Premna pubescens) is one of the plants in Indonesia which is believed to have medicinal properties, but it is not certain. This plant is consumed by the Malay community, one of the tribes in North Sumatra - Indonesia, as vegetables, especially during the fasting month. This paper describes the effects of ethanol extract of leaves of Buas buas as an immunostimulant in rats (Rattus norvegicus). The experiments were performed on 24 tail of male rats, which were three months old, weighing 140-180 g. There were four groups of experiment ie Group A0 was given Carboxy Methyl Cellulose mice (CMC) (control group), group A1 was given 500 mg/ kg bw of Ethanol Extracts of Buas buas. Group A2, given ethanol extracts of Buas buas 500 mg / kg bw + SRBC, and the group A3 only given Sheep Red Blood Cell (SRBC). After this experiment, apigenin levels were analyzed by High Performance Liquid Chromatography (HPLC) Agilent 1220. Then the activity of immunostimulation is determinated by measuring the leukocyte count, percentage of lymphocytes, antibody titers, the levels of immunoglobulin M (IgM), immunoglobulin G (IgG), and lysozyme with ABX Micros 60. The group A2 is seen the highest group of in the number of leukocytes, antibody titer levels, imonoglobulin G (IgG), and imonoglobulin M. The amount of lysozyme owned group A2 is 0:04 ug / ml, whereas other treatments to obtain results that are not different relative significantly. Provision of Ethanol Extracts of Buas buas on mice can increase the leukocytes, lymphocytes, antibody titers, IgM, IgG and Lysozyme.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - Study of the Extract Activities of Buas buas Leaves (Premna pubescens) as Immunostimulant on Rats (Rattus novegicus)
    AU  - Martina Restuati
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    DO  - 10.11648/j.ajbio.20140206.19
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    JF  - American Journal of BioScience
    JO  - American Journal of BioScience
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    PB  - Science Publishing Group
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    UR  - https://doi.org/10.11648/j.ajbio.20140206.19
    AB  - Buas buas (Premna pubescens) is one of the plants in Indonesia which is believed to have medicinal properties, but it is not certain. This plant is consumed by the Malay community, one of the tribes in North Sumatra - Indonesia, as vegetables, especially during the fasting month. This paper describes the effects of ethanol extract of leaves of Buas buas as an immunostimulant in rats (Rattus norvegicus). The experiments were performed on 24 tail of male rats, which were three months old, weighing 140-180 g. There were four groups of experiment ie Group A0 was given Carboxy Methyl Cellulose mice (CMC) (control group), group A1 was given 500 mg/ kg bw of Ethanol Extracts of Buas buas. Group A2, given ethanol extracts of Buas buas 500 mg / kg bw + SRBC, and the group A3 only given Sheep Red Blood Cell (SRBC). After this experiment, apigenin levels were analyzed by High Performance Liquid Chromatography (HPLC) Agilent 1220. Then the activity of immunostimulation is determinated by measuring the leukocyte count, percentage of lymphocytes, antibody titers, the levels of immunoglobulin M (IgM), immunoglobulin G (IgG), and lysozyme with ABX Micros 60. The group A2 is seen the highest group of in the number of leukocytes, antibody titer levels, imonoglobulin G (IgG), and imonoglobulin M. The amount of lysozyme owned group A2 is 0:04 ug / ml, whereas other treatments to obtain results that are not different relative significantly. Provision of Ethanol Extracts of Buas buas on mice can increase the leukocytes, lymphocytes, antibody titers, IgM, IgG and Lysozyme.
    VL  - 2
    IS  - 6
    ER  - 

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Author Information
  • Biology Education Department, Faculty of Mathematic and Science, State University of Medan, Medan, Indonesia

  • Biology Department, Faculty of Mathematic and Science, University of North Sumatra, Medan, Indonesia

  • Biology Department, Faculty of Mathematic and Science, University of North Sumatra, Medan, Indonesia

  • Biology Education Department, Faculty of Mathematic and Science, State University of Medan, Medan, Indonesia

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