| Peer-Reviewed

Protective Effect of Pseudomonas Fluorescens as a Probiotic in Controlling Fish Pathogens

Received: 31 July 2014     Accepted: 27 August 2014     Published: 10 September 2014
Views:       Downloads:
Abstract

The use of Pseudomonas fluorscens isolates as biological control agents against two fish pathogens; Pseudomonas angulliseptica and Streptococcus faecium in Nile tilapia were investigated in vivo and vitro. Pseudomonas fluorscens biovars I, II & III were tested in vitro using the agar diffusion method and showed effectiveness in inhibiting growth of P. angulliseptica and Strep. faecium. Pseudomonas fluorscens biovar II was the most effective with largest inhibition zones against both pathogens. For oral administration, P. fluorscens biovars were incorporated into the mixed feed diet. A significant reduction in mortality rate and a significant increase in hematological parameters, total protein, and globulin in fish groups fed Pseudomonas fluorscens incorporated diet following challenge by P. angulliseptica and Strep. faecium. It could be concluded that P. fluorescens has a protective effect against different Nile Tilapia pathogens and could be used as a probiotic bacteria and an eco-friendly alternative measure to chemical antimicrobials and further research studies to clarify its protective mechanisms on cellular and molecular levels.

Published in American Journal of BioScience (Volume 2, Issue 5)
DOI 10.11648/j.ajbio.20140205.12
Page(s) 175-181
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Probiotics, Pseudomonas, Fish Diseases, Biological Control, Hematological Parameters

References
[1] Daly, J. G. (1999). Other bacterial pathogens. Pages 577-598 in P. T. K. Woo and D. W. Bruno, editors. Fish Diseases and Disorders. Vol. 3, Viral, Bacterial and Fungal infections. CABI Publishing, London, New York.
[2] Austin, B. and Austin, D. A. (2007). Bacterial fish pathogens. Diseases of farmed and wild fish. Springer-Praxis Publishing, Ltd., United Kingdom.
[3] Wakabayashi, H. and Egusa, S. (1972). Characteristics of Pseudomonas sp. From an epizootic of pond-cultured eels (A. japonica). Bulletin of the Japanese Society for the Science of Fish 38:577-587.
[4] Wiklund, T. and Dalsgaard, I. (1987). Diseased outbreaks caused by Ps. anguilliseptica in finnish fish farms. In: Stenmark, A.; Malmberg, G. (Eds.), Parasites and Diseases in Natural Waters and Aquaculture in Nordic Countries, Swedish Museum of Natural History, Stockholm, p. 131 (Abstract).
[5] Eissa, N. Abou El-Ghiet, E. Shaheen, A. A. and Abbass, A. (2010). Characterization of pseudomonas species isolated from Tilapia (Oreochromis niloticus) in Qaroun and Wadi-El-Rayan lakes, Egypt. Global Veterinaria 5 (2):116-12.
[6] El-Hady, M. and Samy A. A. (2011). Molecular typing of pseudomonas species isolated from some cultured fishes in Egypt. Global Veterinaria 7 (6):576-580.
[7] Romalde, J.L. and Toranzo, A. E. (2002). Molecular approaches for the study and diagnosis of salmonid streptococcosis: C.O. Cunningham (Ed.), Molecular diagnosis of salmonid diseases, Kluwer Academic Publ, Netherlands, 8: 211–223
[8] Shoemaker C, Klesius P (1997) Streptococcal disease problems and control a review. In: Fitzsimmons, K. (Ed.) Tilapia Aquaculture, vol. 2. Northest Regional Agricultural Engineering Service, Ithaca, NY, 671- 682.
[9] Yuasa, K. Kitancharoen, N. Kataoka, Y. Al-Murbaty, F. A. (1999). Streptococcus iniae the causative agent of mass mortality in rabbitfish Siganus canaliculatus in Bahrain. Journal of Aquatic Animal Health 11:87-93.
[10] Eldar A, Horvitcz A, Bercovier H (1997) Development and efficacy of a vaccine against Streptococcus iniae infection in farmed rainbow trout. Veterinary Immunology and Immunopathology 56:175-183.
[11] Eldar A, Perl S, Frelier PF, Bercovier H (1999) Red drum Sciaenops ocellatus mortalities associated with Streptococcus iniae infection. Disease of Aquatic Organism 36:121-127.
[12] Colorni, A. Diamant, D. Eldar, A. Kvitt, H. and Zlotkin, A. (2002). Streptococcus iniae infections in Red Sea cage-cultured and wild fishes. Disease of Aquatic Organism 49:165- 170.
[13] FAO/WHO (2001). Health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. Report of Joint Food and Agriculture Organization of the United Nations/World Health (FAO/WHO) Expert Consultation Report on Evaluation of Health and Nutritional Properties of Probiotics in Food Including Powder Milk with Live Lactic Acid Bacteria, pp. 1–34. Córdoba, Argentina: FAO/WHO.
[14] Irianto, A. and Austin, B. (2002). Use of dead probiotics to control furunculosis in rainbow trout, Oncorhynchus mykiss (Walbaum). Journal of Fish Disease 25:333–342.
[15] Nayak, S. K. (2010). Probiotics and immunity: A fish perspective. Fish and Shellfish Immunology 29:2-14.
[16] Das, B. K. Samal, S. K. Samantaray, B.R. Sethi, S. Pattnaik, P. and Mishra, B. K. (2006). Antagonistic activity of cellular components of Pseudomonas species against Aeromonas hydrophila. Aquaculture 253:17–24.
[17] Abd El-Rhman, A. M. Khattab, Y. E. and Shalaby, A. E. (2009). Micrococcus luteus and Pseudomonas species as probiotics for promoting the growth performance and health of Nile tilapia, Oreochromis niloticus. Fish and Shellfish Immunology 27:175– 180.
[18] Eissa, N. and Abou El-Ghiet, E. N. (2011). Efficacy of Pseudomonas fluorescens as biological control agents against Aeromonas hydrophila infection in Oreochromis niloticus. World Journal of Fish and Marine Sciences. 3 (6):564-569, 201.
[19] Smith, P. and Davey, S. (1993). Evidence for the competitive exclusion of Aeromonas salmonicida from fish with stress inducible furunculosis by fluorescent pseudomonad. Journal of Fish Disease 16 (6):521-524.
[20] Gram, L. (1993). Inhibitory effect against pathogenic and spoilage bacteria of Pseudomonas strains isolated from spoiled and fresh fish. Applied and Environmental Microbiology 59:2197–2203.
[21] Bly, J. E. Quiniou, S. M. Lawson, L. A. and Clem, L. W. (1997). Inhibition of Saprolegnia pathogenic for fish by Pseudomonas fluorescens. Journal of Fish Disease 20:35–40.
[22] Hecker M, Engelmann S, Cordwell SJ (2003) Proteomics of Staphylococcus aureus current state and future challenges. Journal of Chromatography B Analytical Technologies in the Biomedical and Life Sciences 787: 179–195.
[23] Alavandi, S. V. Vijayan, K. K. Santiago, T.C. Poornima, M. Jithendran, K. P. Ali S. A. and Rajan, J. S. (2004). Evaluation of Pseudomonas sp. PM 11 and Vibrio fluvialis PM 17 on immune indices of tiger shrimp, (Penaeus monodon). Fish and Shellfish Immunology 17:115-120.
[24] Korkea-aho, T.L. Heikkinen, J. Thompson, K. D. von Wright, A. and Austin, B. (2011). Pseudomonas sp. M174 inhibits the fish pathogen Flavobacterium psychrophilum. Journal of Applied Microbiology, 111: 266–277.
[25] Ruiz, C.M. Roman. G. and Sánchez, J. L. (1996). A marine bacterial strain effective in producing antagonisms of other bacteria. Aquaculture International 4:289–291.
[26] Schalm, O. (1986). Schalm’s Veterinary Hematology, 4th Edition, 524.
[27] Henry, R. (1964). Colorimetric determination of total protein. In: Clinical Chemistry. New York: Harper and Row Publ. pp. 220-225.
[28] Dumas, B.T. and Biggs, H.G. (1972). Standard methods of clinical chemistry. Academic Press, New York, 344.
[29] Feldman, D. Gagnon, J. Hoffmann, R. and Simpson, J. (1988). Stat view IM.IIV.1.02. The solution for data analysis and presentation graphics, 189.
[30] Gram, L. and Melchiorsen, J. (1996). Interaction between fish spoilage bacteria Pseudomonas sp. and Shewanella putrefaciens in fish extracts and on fish tissue. Journal of applied bacteriology, 80(6):589-595.
[31] Sugita, H. Shibuya, K. Shimooka, H. and Deguchi, Y. (1996). Antibacterial abilities of intestinal bacteria in freshwater cultured fish. Aquaculture 145:195-203.
[32] Gram, L. Løvold, T. Nielsen, J. Melchiorsen, J. and Spanggaard, B. (2001). In vitro antagonism of the probiont Pseudomonas fluorescens strain AH2 against Aeromonas salmonicida does not confer protection of salmon against furunculosis. Aquaculture 199: 1–11.
[33] Hamdan, H. Weller, D. M. and Thomashow, L. S. (1991). Relative importance of fluorescent siderophores and other factors in biological control of Gaeumannomyces graminis var. tritici by Pseudomonas fluorescens 2-79 and M4-80R. Applied and Environmental Microbiolology 57:3270-3277.
[34] Shanahan, P. Osullivan, D. J. Simpson, P. Glennon, J. D. and Ogara, F. (1992). Isolation of 2, 4-diacetylphloroglucinol from a fluorescent pseudomonad and investigation of physiological parameters influencing its production. Applied and Environmental Microbiology 58:353-358.
[35] Loper, J. E. and Buyer, J. S. (1991). Siderophores in microbial interactions on plant surfaces. Molecular Plant–Microbe Interactions 4:5–13
[36] Fgaier, H. and Eberl, H. (2010). A competition model between Pseudomonas fluorescens and pathogens via iron chelation. Journal of Theoretical Biology 21, 263(4):566-578.
[37] Nair, S. and Simidu, U. (1987). Distribution and significance of heterotrophic marine bacteria with antibacterial activity. Applied and Environmental Microbiology 53(12):2957-296.
[38] Champomier-Verges, M. C. and Richard, J. (1994). Antibacterial activity among Pseudomonas strains of meat origin. Letters in Applied Microbiology 18:18-20.
[39] Vijayan, K. K. Singh, I. S. B. Jayaprakash N. S. Alavandi, S. V. Pai, R. Preetha, S. S. Rajan, J. J. S. and Santiago, T. C. (2006). A brackish water isolate of Pseudomonas PS–102, a potential antagonistic bacterium against pathogenic vibrios in penaeid and non–penaeid rearing systems. Aquaculture 251(2–4):192–200.
[40] Radjasa, O. K. Salasia, S. I. O. Sabdono, A. Weise, J. Imhoff, J. F. Lammer, C. & Risk, M. J. (2007). Antibacterial activity of marine bacterium Pseudomonas sp. associated with soft coral Sinularia polydactyla against Streptococcus equi subsp. zooepidemicus. International Journal of Pharmacology, 3(2), 170-174.
[41] Alim, I. and Yuto, K. (2009). Bioactive substances produced by marine isolates of Pseudomonas. Journal of Industrial Microbiology and Biotechnology 36(10):1239-1248.
[42] Uzair, B. Ahmed, N. Kousar, F. and Edwards, D. H. (2006). Isolation and characterization of Pseudomonas strain that inhibit growth of indigenous and clinical isolates. The Internet Journal of Microbiology, 2(2).
[43] Ongena, M. Jourdan, E. Adam, A. Schaefer, M. Budzikiewicz, H. and Thonart P (2008). Amino acids, iron, and growth rate as key factors influencing production of the Pseudomonas putida BTP1 benzylamine derivative involved in systemic resistance induction in different plants. Microbial Ecology 55:280–292.
[44] Aly, S. M. Abd-El-Rahman, A. M. John, G. and Mohamed, F. M. (2008). Characterization of some bacteria isolated from Oreochromis niloticus and their potential use as probiotics. Aquaculture 277:1–6.
[45] Alderman, D. J. and Hastings, T. S. (1998). Antibiotic use in aquaculture: development of antibiotic resistance-potential for consumer health risks. International Journal of Food Science and Technology 33:139– 155.
[46] Treves-Brown, K. M. (2000). Applied Fish Pharmacology. Kluwer Academic Publishers, Dordrecht, Netherlands, 26– 54.
Cite This Article
  • APA Style

    Nour Eissa, ElSayed Abou El-Gheit, Adel A. Shaheen. (2014). Protective Effect of Pseudomonas Fluorescens as a Probiotic in Controlling Fish Pathogens. American Journal of BioScience, 2(5), 175-181. https://doi.org/10.11648/j.ajbio.20140205.12

    Copy | Download

    ACS Style

    Nour Eissa; ElSayed Abou El-Gheit; Adel A. Shaheen. Protective Effect of Pseudomonas Fluorescens as a Probiotic in Controlling Fish Pathogens. Am. J. BioScience 2014, 2(5), 175-181. doi: 10.11648/j.ajbio.20140205.12

    Copy | Download

    AMA Style

    Nour Eissa, ElSayed Abou El-Gheit, Adel A. Shaheen. Protective Effect of Pseudomonas Fluorescens as a Probiotic in Controlling Fish Pathogens. Am J BioScience. 2014;2(5):175-181. doi: 10.11648/j.ajbio.20140205.12

    Copy | Download

  • @article{10.11648/j.ajbio.20140205.12,
      author = {Nour Eissa and ElSayed Abou El-Gheit and Adel A. Shaheen},
      title = {Protective Effect of Pseudomonas Fluorescens as a Probiotic in Controlling Fish Pathogens},
      journal = {American Journal of BioScience},
      volume = {2},
      number = {5},
      pages = {175-181},
      doi = {10.11648/j.ajbio.20140205.12},
      url = {https://doi.org/10.11648/j.ajbio.20140205.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20140205.12},
      abstract = {The use of Pseudomonas fluorscens isolates as biological control agents against two fish pathogens; Pseudomonas angulliseptica and Streptococcus faecium in Nile tilapia were investigated in vivo and vitro. Pseudomonas fluorscens biovars I, II & III were tested in vitro using the agar diffusion method and showed effectiveness in inhibiting growth of P. angulliseptica and Strep. faecium. Pseudomonas fluorscens biovar II was the most effective with largest inhibition zones against both pathogens. For oral administration, P. fluorscens biovars were incorporated into the mixed feed diet. A significant reduction in mortality rate and a significant increase in hematological parameters, total protein, and globulin in fish groups fed Pseudomonas fluorscens incorporated diet following challenge by P. angulliseptica and Strep. faecium. It could be concluded that P. fluorescens has a protective effect against different Nile Tilapia pathogens and could be used as a probiotic bacteria and an eco-friendly alternative measure to chemical antimicrobials and further research studies to clarify its protective mechanisms on cellular and molecular levels.},
     year = {2014}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Protective Effect of Pseudomonas Fluorescens as a Probiotic in Controlling Fish Pathogens
    AU  - Nour Eissa
    AU  - ElSayed Abou El-Gheit
    AU  - Adel A. Shaheen
    Y1  - 2014/09/10
    PY  - 2014
    N1  - https://doi.org/10.11648/j.ajbio.20140205.12
    DO  - 10.11648/j.ajbio.20140205.12
    T2  - American Journal of BioScience
    JF  - American Journal of BioScience
    JO  - American Journal of BioScience
    SP  - 175
    EP  - 181
    PB  - Science Publishing Group
    SN  - 2330-0167
    UR  - https://doi.org/10.11648/j.ajbio.20140205.12
    AB  - The use of Pseudomonas fluorscens isolates as biological control agents against two fish pathogens; Pseudomonas angulliseptica and Streptococcus faecium in Nile tilapia were investigated in vivo and vitro. Pseudomonas fluorscens biovars I, II & III were tested in vitro using the agar diffusion method and showed effectiveness in inhibiting growth of P. angulliseptica and Strep. faecium. Pseudomonas fluorscens biovar II was the most effective with largest inhibition zones against both pathogens. For oral administration, P. fluorscens biovars were incorporated into the mixed feed diet. A significant reduction in mortality rate and a significant increase in hematological parameters, total protein, and globulin in fish groups fed Pseudomonas fluorscens incorporated diet following challenge by P. angulliseptica and Strep. faecium. It could be concluded that P. fluorescens has a protective effect against different Nile Tilapia pathogens and could be used as a probiotic bacteria and an eco-friendly alternative measure to chemical antimicrobials and further research studies to clarify its protective mechanisms on cellular and molecular levels.
    VL  - 2
    IS  - 5
    ER  - 

    Copy | Download

Author Information
  • Aquatic Diseases Laboratory, Aquaculture Division, National Institute of Oceanography and Fisheries, 101 Kaser El-Aini Street, Cairo 11516, Egypt

  • Aquatic Diseases Laboratory, Aquaculture Division, National Institute of Oceanography and Fisheries, 101 Kaser El-Aini Street, Cairo 11516, Egypt

  • Department of Fish Diseases and Management, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh, 13736, Egypt

  • Sections