| Peer-Reviewed

A New Sustainable Symbiotic Association of Lactic Acid Cocci and Bacilli for Colonization/Recolonization of Vagina and Prevention of Bacterial Vaginosis

Received: 7 April 2014     Accepted: 15 April 2014     Published: 30 April 2014
Views:       Downloads:
Abstract

Various factors; nutrition, hygiene, stress, infections, low immunity, menses, frequent sexual intercourses, pregnancy etc., destroyed vaginal microbial balance, which reflected in the number of lactobacilli decrease and increase the number of pathogenic microorganisms, as well as the pH-value. All this in turn causes vaginosis. The aim of this study is creation of symbiotic association of probiotic bacteria (SAPB) with high colonization/recolonization properties for rapid response to vaginal acute and chronic challenges. It is expected that LABs symbiotic association will be more beneficial for vaginal colonization/recoclonization, because the lactococci are growth well at elevate pH 9.0-5.0 and by reducing of vaginal pH promote the growth of lactobacilli, adapted to low pH 6.5-3.5. In this study used lactobacilli biocompatible with cocci possessing high antimicrobial activity from laboratory LABs collection, isolated earlier from 20-45 years old healthy Ar-menian woman volunteers (n=40). And it is the first time that lactococci were used as probiotic in vaginal coloniza-tion/recolonization practice. The symbiotic association of Lactobacillus plantarum (L. plantarum) GH 202 and Lactococcus lactis (L. lactis) GH 204 strains shows higher inhibitory activities against bacterial pathogens and Candida albicans (C. albicans). The SAPB growth rate is more intensive and biomass accumulation is higher, than the mono cultures. It is sustainable at different pH conditions of growth and during multiple subculturing imitating up and down changes of vaginal pH. The SAPB strains have high hydrophobicity evaluated by MATS test and high coaggregation properties essential for increasing their colonization potential, when they used in mixed culture.

Published in American Journal of BioScience (Volume 2, Issue 3)
DOI 10.11648/j.ajbio.20140203.11
Page(s) 84-88
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

LABs Association, Antimicrobial Activity, Adhesion, Coaggregation

References
[1] A. Ma¨yra¨-Ma¨kinen, M. Manninen and H. Gyllenberg, The adherence of lactic acid bacteria to the columnar epithelial cells of pigs and calves. Journal of Applied Microbiology 55, 1983, 241–245.
[2] B. Larsen, G. R. Monif, Understanding the bacterial flora of the female genital tract. Clin Infect Dis, 32, 2001, 69-77.
[3] E. Wolters-Everhardt, J.M.J. Dony, W.H. Doesburg and J.H.M. De Pont, Buffering capacity of human semen. Fertil. Steril. 46, 1986, 114–119.
[4] G. Reid, J. Burton, Use of Lactobacillus to prevent infection by pathogenic bacteria. Microbes Infect, 4, 2002, 319-324.
[5] H. Kimoto, J. Kurisaki, T.M. Tsuji, S. Ohmomo and T. Okamoto, Lactococci as probiotic strains: adhesion to human enterocyte-like Caco-2 cells and tolerance to low pH and bile. Letters in Applied Microbiology 29, 1999, 313–316.
[6] J.P. Lepargneur, V. Rousseau, Protective role of the Doderlein flora. J Gynecol Obstet Biol Reprod, 31, 2002, 485-494.
[7] M.N. Bellon-Fontaine, J. Rault and C.J. van Oss, Microbial adhesion to solvents: a novel method to determine the electrondonor/electron-acceptor or Lewis acid-base properties of microbialcells. Colloids and Surfaces 7, 1996, 47–53.
[8] O. Tresse, V. Lebret, T. Benezech, C. Faille, Comparative evaluation of adhesion, surface properties, and surface protein composition of Listeria monocytogenes strains after cultivation at constant pH of 5 and 7. Journal of Applied Microbiology, Oxford, v. 101, n. 1, 2006, 53–62.
[9] P. Marteau, J. C. Rambaud, Potential of using lactic acid bacteria for therapy and immunomodulation in man. FEMS Microbiol Rev., 12, 1993, 207-220.
[10] P.L. Conway and S. Kjellberg, Protein-mediated adhesion of Lactobacillus fermentum strain 737 to mouse stomach squamous epithelium. Journal of General Microbiology 135, 1989, 1175–1186.
[11] P.R. Murray, E.J. Baron, J.H. Jorgensen, M.A. Pfaller, R.H. Yolken, Manual of Clinical Microbiology, 8th Edition, ASM, Washington, D.C., 2003.
[12] P.S. Handley, D.W.S. Harty, J.E. Wyatt, C.R. Brown, J.P. Doran and A.C.C. Gibbs, A comparison of the adhesion, coaggregation and cell-surface hydrophobicity properties of fibrilar and fimbriate strains of Streptococcus salivarius. Journal of General Microbiology 133, 1987, 3207–3217.
[13] R.W. Jack, J.R. Tagg and B. Ray, Microbiol. Rev., 59, 2, 1995, 171-200.
[14] S.D. Todorov, M. Botes, S.T. Danova and L.M.T. Dicks, Probiotic properties of Lactococcus lactis ssp. lactis HV219, isolated from human vaginal secretions, Journal of Applied Microbiology 103, 2007, 629–639.
[15] S.S. Witkin, Bacterial flora of the female genital tract: function and immune regulation, Best Practice & Research Clinical Obstetrics and Gynecology, Vol. 21, No. 3, 2007, 347-354.
[16] S.V. Veen, D.T. Abee, Mixed species biofilms of Listeria monocytogenes and Lactobacillus plantarum show enhanced resistance to benzalkonium chloride and peracetic acid. International Journal of Food Microbiology, Amsterdam, v. 144, n. 3, 2011, 421–431.
[17] V. Redondo-Lopez, R.L. Cook, J.D. Sobel, Emerging role of lactobacilli in the control and maintenance of the vaginal bacterial microflora, Rev Infect Dis, 12, 1990, 856-872.
[18] V.L. Crow and P.K. Gopal, Cell surface differences of lactococcal strains. International Dairy Journal 5, 1995, 45–68.
[19] W.H. Masters and V. E. Johnson, In: Human sexual response, Bantam Books, Boston, Mass. 1966.
Cite This Article
  • APA Style

    Hrachya Garegin Hovhannisyan, Gohar Gagik Grigoryan. (2014). A New Sustainable Symbiotic Association of Lactic Acid Cocci and Bacilli for Colonization/Recolonization of Vagina and Prevention of Bacterial Vaginosis. American Journal of BioScience, 2(3), 84-88. https://doi.org/10.11648/j.ajbio.20140203.11

    Copy | Download

    ACS Style

    Hrachya Garegin Hovhannisyan; Gohar Gagik Grigoryan. A New Sustainable Symbiotic Association of Lactic Acid Cocci and Bacilli for Colonization/Recolonization of Vagina and Prevention of Bacterial Vaginosis. Am. J. BioScience 2014, 2(3), 84-88. doi: 10.11648/j.ajbio.20140203.11

    Copy | Download

    AMA Style

    Hrachya Garegin Hovhannisyan, Gohar Gagik Grigoryan. A New Sustainable Symbiotic Association of Lactic Acid Cocci and Bacilli for Colonization/Recolonization of Vagina and Prevention of Bacterial Vaginosis. Am J BioScience. 2014;2(3):84-88. doi: 10.11648/j.ajbio.20140203.11

    Copy | Download

  • @article{10.11648/j.ajbio.20140203.11,
      author = {Hrachya Garegin Hovhannisyan and Gohar Gagik Grigoryan},
      title = {A New Sustainable Symbiotic Association of Lactic Acid Cocci and Bacilli for Colonization/Recolonization of Vagina and Prevention of Bacterial Vaginosis},
      journal = {American Journal of BioScience},
      volume = {2},
      number = {3},
      pages = {84-88},
      doi = {10.11648/j.ajbio.20140203.11},
      url = {https://doi.org/10.11648/j.ajbio.20140203.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20140203.11},
      abstract = {Various factors; nutrition, hygiene, stress, infections, low immunity, menses, frequent sexual intercourses, pregnancy etc., destroyed vaginal microbial balance, which reflected in the number of lactobacilli decrease and increase the number of pathogenic microorganisms, as well as the pH-value. All this in turn causes vaginosis. The aim of this study is creation of symbiotic association of probiotic bacteria (SAPB) with high colonization/recolonization properties for rapid response to vaginal acute and chronic challenges. It is expected that LABs symbiotic association will be more beneficial for vaginal colonization/recoclonization, because the lactococci are growth well at elevate pH 9.0-5.0 and by reducing of vaginal pH promote the growth of lactobacilli, adapted to low pH 6.5-3.5. In this study used lactobacilli biocompatible with cocci possessing high antimicrobial activity from laboratory LABs collection, isolated earlier from 20-45 years old healthy Ar-menian woman volunteers (n=40). And it is the first time that lactococci were used as probiotic in vaginal coloniza-tion/recolonization practice. The symbiotic association of Lactobacillus plantarum (L. plantarum) GH 202 and Lactococcus lactis (L. lactis) GH 204 strains shows higher inhibitory activities against bacterial pathogens and Candida albicans (C. albicans). The SAPB growth rate is more intensive and biomass accumulation is higher, than the mono cultures. It is sustainable at different pH conditions of growth and during multiple subculturing imitating up and down changes of vaginal pH. The SAPB strains have high hydrophobicity evaluated by MATS test and high coaggregation properties essential for increasing their colonization potential, when they used in mixed culture.},
     year = {2014}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - A New Sustainable Symbiotic Association of Lactic Acid Cocci and Bacilli for Colonization/Recolonization of Vagina and Prevention of Bacterial Vaginosis
    AU  - Hrachya Garegin Hovhannisyan
    AU  - Gohar Gagik Grigoryan
    Y1  - 2014/04/30
    PY  - 2014
    N1  - https://doi.org/10.11648/j.ajbio.20140203.11
    DO  - 10.11648/j.ajbio.20140203.11
    T2  - American Journal of BioScience
    JF  - American Journal of BioScience
    JO  - American Journal of BioScience
    SP  - 84
    EP  - 88
    PB  - Science Publishing Group
    SN  - 2330-0167
    UR  - https://doi.org/10.11648/j.ajbio.20140203.11
    AB  - Various factors; nutrition, hygiene, stress, infections, low immunity, menses, frequent sexual intercourses, pregnancy etc., destroyed vaginal microbial balance, which reflected in the number of lactobacilli decrease and increase the number of pathogenic microorganisms, as well as the pH-value. All this in turn causes vaginosis. The aim of this study is creation of symbiotic association of probiotic bacteria (SAPB) with high colonization/recolonization properties for rapid response to vaginal acute and chronic challenges. It is expected that LABs symbiotic association will be more beneficial for vaginal colonization/recoclonization, because the lactococci are growth well at elevate pH 9.0-5.0 and by reducing of vaginal pH promote the growth of lactobacilli, adapted to low pH 6.5-3.5. In this study used lactobacilli biocompatible with cocci possessing high antimicrobial activity from laboratory LABs collection, isolated earlier from 20-45 years old healthy Ar-menian woman volunteers (n=40). And it is the first time that lactococci were used as probiotic in vaginal coloniza-tion/recolonization practice. The symbiotic association of Lactobacillus plantarum (L. plantarum) GH 202 and Lactococcus lactis (L. lactis) GH 204 strains shows higher inhibitory activities against bacterial pathogens and Candida albicans (C. albicans). The SAPB growth rate is more intensive and biomass accumulation is higher, than the mono cultures. It is sustainable at different pH conditions of growth and during multiple subculturing imitating up and down changes of vaginal pH. The SAPB strains have high hydrophobicity evaluated by MATS test and high coaggregation properties essential for increasing their colonization potential, when they used in mixed culture.
    VL  - 2
    IS  - 3
    ER  - 

    Copy | Download

Author Information
  • “Armbiotechnology” S&P Center of National Academy of Sciences of Armenia, Yerevan, Armenia

  • “Armbiotechnology” S&P Center of National Academy of Sciences of Armenia, Yerevan, Armenia

  • Sections