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Survey of Lysogenic Phages in the 72 Strains of Escherichia coli Collection of Reference (ECOR) and Identification of a Phage Derived from the ECOR52 Strain

Received: 29 January 2014     Published: 28 February 2014
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Abstract

Escherichia coli collection of reference (ECOR) is a standard collection of 72 wild-type E. coli strains that represent natural E. coli populations found in various environments. Although these strains are widely used in experiments investigating the physiology and behavior of wild-type E. coli, their genetic features including accessory DNA have not been sufficiently studied. In this study, we surveyed for the presence of lysogenic phages in each ECOR strain under both inducing and non-inducing conditions. We found that 34 strains could produce plaque-forming phages; among them, 14 strains were newly discovered to harbor lysogenic phages capable of entering the lytic cycle. We isolated a new phage (designated as “MSU52-L1”) from the ECOR52 strain and identified it as a P22/lambda-like phage with homology to known phages, such as CUS-3, HK620, and HK140.

Published in American Journal of BioScience (Volume 2, Issue 2)
DOI 10.11648/j.ajbio.20140202.12
Page(s) 32-37
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Bacteriophage, Escherichia coli Collection of Reference, Lambdoid Phage, P22

References
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[10] B. J. Bachmann, "Derivations and genotypes of some mutant derivatives of Escherichia coli K-12," In: F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low Jr, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, H. E. Umbarger Eds. Escherichia coli and Salmonella typhimurium Cellular and Molecular Biology. 2nd ed. Washington, D.C., ASM Press. 1996; 2460-2488.
[11] S. Takeshita, M. Sato, M. Toba, and W. Masahashi, "Hashimoto-Gotoh T. High-copy-number and low-copy-number plasmid vectors for lacZ α-complementation and chloramphenicol-or kanamycin-resistance selection," Gene, vol. 61, pp. 63-74, 1987.
[12] C. Yanisch-Perron, J. Vieira, and J. Messing. "Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors," Gene, vol. 33, pp. 103-119, 1985.
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Cite This Article
  • APA Style

    Yuka Shibata, Chisato Ugumori, Anna Takahashi, Ayuka Sekoguchi, Sumio Maeda. (2014). Survey of Lysogenic Phages in the 72 Strains of Escherichia coli Collection of Reference (ECOR) and Identification of a Phage Derived from the ECOR52 Strain. American Journal of BioScience, 2(2), 32-37. https://doi.org/10.11648/j.ajbio.20140202.12

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    ACS Style

    Yuka Shibata; Chisato Ugumori; Anna Takahashi; Ayuka Sekoguchi; Sumio Maeda. Survey of Lysogenic Phages in the 72 Strains of Escherichia coli Collection of Reference (ECOR) and Identification of a Phage Derived from the ECOR52 Strain. Am. J. BioScience 2014, 2(2), 32-37. doi: 10.11648/j.ajbio.20140202.12

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    AMA Style

    Yuka Shibata, Chisato Ugumori, Anna Takahashi, Ayuka Sekoguchi, Sumio Maeda. Survey of Lysogenic Phages in the 72 Strains of Escherichia coli Collection of Reference (ECOR) and Identification of a Phage Derived from the ECOR52 Strain. Am J BioScience. 2014;2(2):32-37. doi: 10.11648/j.ajbio.20140202.12

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  • @article{10.11648/j.ajbio.20140202.12,
      author = {Yuka Shibata and Chisato Ugumori and Anna Takahashi and Ayuka Sekoguchi and Sumio Maeda},
      title = {Survey of Lysogenic Phages in the 72 Strains of Escherichia coli Collection of Reference (ECOR) and Identification of a Phage Derived from the ECOR52 Strain},
      journal = {American Journal of BioScience},
      volume = {2},
      number = {2},
      pages = {32-37},
      doi = {10.11648/j.ajbio.20140202.12},
      url = {https://doi.org/10.11648/j.ajbio.20140202.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20140202.12},
      abstract = {Escherichia coli collection of reference (ECOR) is a standard collection of 72 wild-type E. coli strains that represent natural E. coli populations found in various environments. Although these strains are widely used in experiments investigating the physiology and behavior of wild-type E. coli, their genetic features including accessory DNA have not been sufficiently studied. In this study, we surveyed for the presence of lysogenic phages in each ECOR strain under both inducing and non-inducing conditions. We found that 34 strains could produce plaque-forming phages; among them, 14 strains were newly discovered to harbor lysogenic phages capable of entering the lytic cycle. We isolated a new phage (designated as “MSU52-L1”) from the ECOR52 strain and identified it as a P22/lambda-like phage with homology to known phages, such as CUS-3, HK620, and HK140.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - Survey of Lysogenic Phages in the 72 Strains of Escherichia coli Collection of Reference (ECOR) and Identification of a Phage Derived from the ECOR52 Strain
    AU  - Yuka Shibata
    AU  - Chisato Ugumori
    AU  - Anna Takahashi
    AU  - Ayuka Sekoguchi
    AU  - Sumio Maeda
    Y1  - 2014/02/28
    PY  - 2014
    N1  - https://doi.org/10.11648/j.ajbio.20140202.12
    DO  - 10.11648/j.ajbio.20140202.12
    T2  - American Journal of BioScience
    JF  - American Journal of BioScience
    JO  - American Journal of BioScience
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    SN  - 2330-0167
    UR  - https://doi.org/10.11648/j.ajbio.20140202.12
    AB  - Escherichia coli collection of reference (ECOR) is a standard collection of 72 wild-type E. coli strains that represent natural E. coli populations found in various environments. Although these strains are widely used in experiments investigating the physiology and behavior of wild-type E. coli, their genetic features including accessory DNA have not been sufficiently studied. In this study, we surveyed for the presence of lysogenic phages in each ECOR strain under both inducing and non-inducing conditions. We found that 34 strains could produce plaque-forming phages; among them, 14 strains were newly discovered to harbor lysogenic phages capable of entering the lytic cycle. We isolated a new phage (designated as “MSU52-L1”) from the ECOR52 strain and identified it as a P22/lambda-like phage with homology to known phages, such as CUS-3, HK620, and HK140.
    VL  - 2
    IS  - 2
    ER  - 

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Author Information
  • Graduate School of Humanities and Sciences, Nara Women’s University, Nara, Japan

  • Faculty of Human Life and Environment, Nara Women’s University, Nara, Japan

  • Faculty of Human Life and Environment, Nara Women’s University, Nara, Japan

  • Graduate School of Humanities and Sciences, Nara Women’s University, Nara, Japan

  • Graduate School of Humanities and Sciences, Nara Women’s University, Nara, Japan

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