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Molecular and Biological Studies of Papaya Ringspot Virus Isolates from Brazil and Cuba

Received: 6 August 2014     Accepted: 19 August 2014     Published: 30 August 2014
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Abstract

The coat protein genes of 21 Papaya ringspot virus (PRSV) isolates from Brazil and 7 isolates from Cuba were sequenced and analyzed, and the symptoms induced by the virus isolates were evaluated in papaya (Carica papaya L. cv. Solo) and squash (Cucurbita pepo L. cv. Caserta). The virus isolates were grouped in phylogenetic trees by geographic region, presenting the highest nucleotide and amino acid identities with those isolates collected in the same regions. The American and Indian isolates, from GenBank were grouped together in the tree, and the Brazilian isolates were grouped by state; the Cuban isolates from the eastern region were closer to the American isolates than to those from the central-west region. Typical mosaic symptoms, grouped according to intensity, were observed in the inoculated papaya and squash seedlings. The variability of the coat protein genes confirmed that virus control using cross-protection and transgenic plants requires the selection of region-specific virus isolates in each country.

Published in American Journal of Agriculture and Forestry (Volume 2, Issue 5)
DOI 10.11648/j.ajaf.20140205.11
Page(s) 209-218
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

PRSV-P, Coat protein, Phylogeny, Papaya, Squash, Symptom intensity

References
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Cite This Article
  • APA Style

    Douglas Rodríguez Martínez, Priscilla de Sousa Geraldino Duarte, Justo González Olmedo, Antonia dos Reis Figueira. (2014). Molecular and Biological Studies of Papaya Ringspot Virus Isolates from Brazil and Cuba. American Journal of Agriculture and Forestry, 2(5), 209-218. https://doi.org/10.11648/j.ajaf.20140205.11

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    ACS Style

    Douglas Rodríguez Martínez; Priscilla de Sousa Geraldino Duarte; Justo González Olmedo; Antonia dos Reis Figueira. Molecular and Biological Studies of Papaya Ringspot Virus Isolates from Brazil and Cuba. Am. J. Agric. For. 2014, 2(5), 209-218. doi: 10.11648/j.ajaf.20140205.11

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    AMA Style

    Douglas Rodríguez Martínez, Priscilla de Sousa Geraldino Duarte, Justo González Olmedo, Antonia dos Reis Figueira. Molecular and Biological Studies of Papaya Ringspot Virus Isolates from Brazil and Cuba. Am J Agric For. 2014;2(5):209-218. doi: 10.11648/j.ajaf.20140205.11

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  • @article{10.11648/j.ajaf.20140205.11,
      author = {Douglas Rodríguez Martínez and Priscilla de Sousa Geraldino Duarte and Justo González Olmedo and Antonia dos Reis Figueira},
      title = {Molecular and Biological Studies of Papaya Ringspot Virus Isolates from Brazil and Cuba},
      journal = {American Journal of Agriculture and Forestry},
      volume = {2},
      number = {5},
      pages = {209-218},
      doi = {10.11648/j.ajaf.20140205.11},
      url = {https://doi.org/10.11648/j.ajaf.20140205.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20140205.11},
      abstract = {The coat protein genes of 21 Papaya ringspot virus (PRSV) isolates from Brazil and 7 isolates from Cuba were sequenced and analyzed, and the symptoms induced by the virus isolates were evaluated in papaya (Carica papaya L. cv. Solo) and squash (Cucurbita pepo L. cv. Caserta). The virus isolates were grouped in phylogenetic trees by geographic region, presenting the highest nucleotide and amino acid identities with those isolates collected in the same regions. The American and Indian isolates, from GenBank were grouped together in the tree, and the Brazilian isolates were grouped by state; the Cuban isolates from the eastern region were closer to the American isolates than to those from the central-west region. Typical mosaic symptoms, grouped according to intensity, were observed in the inoculated papaya and squash seedlings. The variability of the coat protein genes confirmed that virus control using cross-protection and transgenic plants requires the selection of region-specific virus isolates in each country.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - Molecular and Biological Studies of Papaya Ringspot Virus Isolates from Brazil and Cuba
    AU  - Douglas Rodríguez Martínez
    AU  - Priscilla de Sousa Geraldino Duarte
    AU  - Justo González Olmedo
    AU  - Antonia dos Reis Figueira
    Y1  - 2014/08/30
    PY  - 2014
    N1  - https://doi.org/10.11648/j.ajaf.20140205.11
    DO  - 10.11648/j.ajaf.20140205.11
    T2  - American Journal of Agriculture and Forestry
    JF  - American Journal of Agriculture and Forestry
    JO  - American Journal of Agriculture and Forestry
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    EP  - 218
    PB  - Science Publishing Group
    SN  - 2330-8591
    UR  - https://doi.org/10.11648/j.ajaf.20140205.11
    AB  - The coat protein genes of 21 Papaya ringspot virus (PRSV) isolates from Brazil and 7 isolates from Cuba were sequenced and analyzed, and the symptoms induced by the virus isolates were evaluated in papaya (Carica papaya L. cv. Solo) and squash (Cucurbita pepo L. cv. Caserta). The virus isolates were grouped in phylogenetic trees by geographic region, presenting the highest nucleotide and amino acid identities with those isolates collected in the same regions. The American and Indian isolates, from GenBank were grouped together in the tree, and the Brazilian isolates were grouped by state; the Cuban isolates from the eastern region were closer to the American isolates than to those from the central-west region. Typical mosaic symptoms, grouped according to intensity, were observed in the inoculated papaya and squash seedlings. The variability of the coat protein genes confirmed that virus control using cross-protection and transgenic plants requires the selection of region-specific virus isolates in each country.
    VL  - 2
    IS  - 5
    ER  - 

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Author Information
  • Department of Plant Pathology, Federal University of Lavras, Lavras, Brazil

  • Department of Plant Pathology, Federal University of Lavras, Lavras, Brazil

  • Bioplants Center, University of Ciego de Avila, Ciego de Avila, Cuba

  • Department of Plant Pathology, Federal University of Lavras, Lavras, Brazil

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