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Isolation of Microflora in Earthworms Guts from Different Substrates

Received: 19 July 2014     Accepted: 31 July 2014     Published: 20 August 2014
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Abstract

The fungi and bacteria were analysed in the gut of Eudrilus eugeniae, from different feed substrates like dry leaves, plastic waste, kitchen waste and waste paper. Certain species of fungi (Saksenae vasiformis, Mucor plumbeus, Cladosporium carrionii, C. herbacium, Alternaria sp., Cunninghamella echinulata, Mycetia sterila, Syncephalostrum racemosum, Curvalaria lunata, C. geniculata and Geotrichum candidum) and bacteria (Pseudomonas aeruginosa, Mima polymorpha, E. cloacae, Proteus vulgaris, P. mirabilis, P. rettgeri, Escherichia coli, Staphylococus citreus, B. cereus, Enterococci and Micrococci) were completely digested. Certain other species were not digested fungi like Aspergillus fumigatus, A. flavus, The microbial proliferation was more in the casts, due to the environment prevailing - rich in nutrient supply and large surface area available for growth and reproduction of the microbes that lead to enhanced microbial activity and humic acid contents in the casts.

Published in American Journal of Agriculture and Forestry (Volume 2, Issue 4)
DOI 10.11648/j.ajaf.20140204.24
Page(s) 206-208
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Microflora, Earthworm gut, Microbial Population, Dry Leaves, Plastic Waste, Kitchen Waste and Waste Paper

References
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[2] Anonymous, A.: Difco manual of dehydrated culture media and reagent for microbiological and Clinical Laboratories. Inc. Detroit. Michigan, 1977, pp.350.
[3] Mahon, R.C. and Manuselis, J.R.: Utilization of colonial morphology for the presumptive identification of microorganisms In: Text book of Diagnostic Microbiology (Eds: Mahon, R.C. and Manuselis, J.R.). W.B. Saunders Company, Pennsylvania,1995, pp. 307-321.
[4] Kwon Chung, J.K. and Bennett, E.J.: Laboratory diagnosis. In: Medical mycology., Chap 3. pp. 44-71. Lea & Febiger, Philadelphia, London, 1992.
[5] Parthasarathi, K. and Ranganathan, L.S.: Influence of press mud on the development of the ovary, oogenesis and the neurosecretory cells of the earthworm, Eudrilus eugeniae (Kinberg). African Zool., 2000;35(2): 281-286.
[6] Edwards, C.A and Fletcher, K.E.: Interaction between earthworms and microorganisms in organic matter breakdown. Agri. Ecosyst. Environ., 1988;24: 235-247.
[7] Dash, H.K., Beura, B.N. and Dash, M.C.: Gut load, transit time, gut microflora and turnover of soil, plant and fungal material by some tropical earthworms. Pedobiologia, 1986;29: 13-20.
[8] Ghosh, N., Basu, S. and Behera, N.: Microfungi in the gut and cast of Perionyx millardi, a tropical earthworm. J. Soil Biol. Ecol., 1989; 9(1): 46-50.
[9] Kozlovskaya, L.S. and Zhdannikova, E.N.: Joint action of earthworms and microflora in forest soils. Dokl. Akad. Nauk SSSR, 1961;139: 470- 473.
[10] Edwards, C.A. and Bohlen, P.J.: Biology and ecology of earthworms, 3rd Ed., Chapman and Hall, London, 1996 .
[11] Cai, H., Zarda, B., Mattison, R.G., Schonholzer, F. and Hahn, D.: Fate of protozoa transiting the digestive tract of the earthworm Lumbricus terrestris L. Pedobiologia, 2002;46:161-175.
[12] Stevenson, F.J.: Humus chemistry. Genesis, composition and reactions, John Wiley and Sons Incorporation, New York, 1994.
[13] Filip, Z., Pecher, W. and Berthelin, J.: Microbial utilization and transformation of humic acids extracted from different soils. J. Plant Nutri. Soil Sci., 1999;162: 215-222 .
[14] Rovira, S.P.A., Brunetti, G., Polo, P. and Senesi, N.: Comparative chemical and spectroscopic characterization of humic acids from sewage sludges and sludge amended soils. Soil Sci., 2002;167(4): 235-245.
[15] Manivannan, S., Ramamoorthy, P., Parthasarathi, K. and Ranganathan, L.S.: Effect of sugar industrial wastes on the growth and reproduction of earthworms. India J. Exp. Zool., 2004;7(1): 29-37.
Cite This Article
  • APA Style

    Mohamed Omer Albasha, P. Gupta, P. W. Ramteke. (2014). Isolation of Microflora in Earthworms Guts from Different Substrates. American Journal of Agriculture and Forestry, 2(4), 206-208. https://doi.org/10.11648/j.ajaf.20140204.24

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    ACS Style

    Mohamed Omer Albasha; P. Gupta; P. W. Ramteke. Isolation of Microflora in Earthworms Guts from Different Substrates. Am. J. Agric. For. 2014, 2(4), 206-208. doi: 10.11648/j.ajaf.20140204.24

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    AMA Style

    Mohamed Omer Albasha, P. Gupta, P. W. Ramteke. Isolation of Microflora in Earthworms Guts from Different Substrates. Am J Agric For. 2014;2(4):206-208. doi: 10.11648/j.ajaf.20140204.24

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  • @article{10.11648/j.ajaf.20140204.24,
      author = {Mohamed Omer Albasha and P. Gupta and P. W. Ramteke},
      title = {Isolation of Microflora in Earthworms Guts from Different Substrates},
      journal = {American Journal of Agriculture and Forestry},
      volume = {2},
      number = {4},
      pages = {206-208},
      doi = {10.11648/j.ajaf.20140204.24},
      url = {https://doi.org/10.11648/j.ajaf.20140204.24},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20140204.24},
      abstract = {The fungi and bacteria were analysed in the gut of Eudrilus eugeniae, from different feed substrates like dry leaves, plastic waste, kitchen waste and waste paper. Certain species of fungi (Saksenae vasiformis, Mucor plumbeus, Cladosporium carrionii, C. herbacium, Alternaria sp., Cunninghamella echinulata, Mycetia sterila, Syncephalostrum racemosum, Curvalaria lunata, C. geniculata and Geotrichum candidum) and bacteria (Pseudomonas aeruginosa, Mima polymorpha, E. cloacae, Proteus vulgaris, P. mirabilis, P. rettgeri, Escherichia coli, Staphylococus citreus, B. cereus, Enterococci and Micrococci) were completely digested. Certain other species were not digested fungi like Aspergillus fumigatus, A. flavus, The microbial proliferation was more in the casts, due to the environment prevailing - rich in nutrient supply and large surface area available for growth and reproduction of the microbes that lead to enhanced microbial activity and humic acid contents in the casts.},
     year = {2014}
    }
    

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    T1  - Isolation of Microflora in Earthworms Guts from Different Substrates
    AU  - Mohamed Omer Albasha
    AU  - P. Gupta
    AU  - P. W. Ramteke
    Y1  - 2014/08/20
    PY  - 2014
    N1  - https://doi.org/10.11648/j.ajaf.20140204.24
    DO  - 10.11648/j.ajaf.20140204.24
    T2  - American Journal of Agriculture and Forestry
    JF  - American Journal of Agriculture and Forestry
    JO  - American Journal of Agriculture and Forestry
    SP  - 206
    EP  - 208
    PB  - Science Publishing Group
    SN  - 2330-8591
    UR  - https://doi.org/10.11648/j.ajaf.20140204.24
    AB  - The fungi and bacteria were analysed in the gut of Eudrilus eugeniae, from different feed substrates like dry leaves, plastic waste, kitchen waste and waste paper. Certain species of fungi (Saksenae vasiformis, Mucor plumbeus, Cladosporium carrionii, C. herbacium, Alternaria sp., Cunninghamella echinulata, Mycetia sterila, Syncephalostrum racemosum, Curvalaria lunata, C. geniculata and Geotrichum candidum) and bacteria (Pseudomonas aeruginosa, Mima polymorpha, E. cloacae, Proteus vulgaris, P. mirabilis, P. rettgeri, Escherichia coli, Staphylococus citreus, B. cereus, Enterococci and Micrococci) were completely digested. Certain other species were not digested fungi like Aspergillus fumigatus, A. flavus, The microbial proliferation was more in the casts, due to the environment prevailing - rich in nutrient supply and large surface area available for growth and reproduction of the microbes that lead to enhanced microbial activity and humic acid contents in the casts.
    VL  - 2
    IS  - 4
    ER  - 

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Author Information
  • Department of Zoology, Alejelat Faculty of Science, Zawia University, Zawia, Libya

  • Department of Biological Science, Faculty of Science, Sam Higgingbottom Institute of Agricultural, Technology & Science(SHIATS), Allahabad, India

  • Department of Biological Science, Faculty of Science, Sam Higgingbottom Institute of Agricultural, Technology & Science(SHIATS), Allahabad, India

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