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Induction of Callus and Somatic Embryogenesis from Cotyledon and Leaf Explants of Yeheb (Cordeauxia edulis Hemsl)

Received: 23 November 2014     Accepted: 11 December 2014     Published: 22 December 2014
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Abstract

‘Yeheb’ (Cordeauxia edulis Hemsl) is a multipurpose and evergreen shrub and endemic to southeastern corner of Ethiopia and Somalia. It is adapted to low and irregular rainfall and survives a very long dry season. It has enormous economic and food security roles to the pastoralist of Somali Region State in Ethiopia. However, the plant is threatened with extinction due to over exploitation and its’ poor natural regeneration capacity. The aim of this was to explore the potential for in vitro rapid regeneration of ‘yeheb’ from cotyledon and leaf explants on Murashige and Skoog (MS) media supplemented with 1.0 – 8.0 mg l-1concentrations of 2, 4-D for callus induction and 2.0 and 3.0 mg l-1concentration of N6-benzylaminopurine (BAP), thidiazuron (TDZ) and kinetin (Kin) with combination of 4.0 mg l-1 of 2, 4-Dichlorophenyl acetic acid (2, 4-D) for embryo induction. The result of these studies revealed that the highest percentage of callus induction (89%) were obtained from both leaf and cotyledon explants on MS media supplemented with 4.00 and 8.00 mg l-1 2, 4-D, respectively. The highest percentage of embryo regeneration responses (88.89 and 77.78%) were obtained from leaf and cotyledon explants on same media: MS media supplemented by 3.00 mg l-1 TDZ +4.00 mg l-1 2, 4-D. As a conclusion; this is the first attempt for callus and embryo in vitro regeneration of C. edulis and permissible result for mass propagation and cryopreservation.

Published in Agriculture, Forestry and Fisheries (Volume 3, Issue 6)
DOI 10.11648/j.aff.20140306.15
Page(s) 464-468
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Callus, Cotyledon, Embryo, Explants, In Vitro

References
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[3] Miège, J. and Miège, M.N., 1978 Cordeauxia edulis a Caesalpinaceae of Arid Zones of East Africa, Caryologic, blastogenic and biochemical features. Potential aspects for nutrition. Economic Botany, 32: 336-345.
[4] Booth, F.E.M. and Wickens, G.E., 1988. Non-timber uses of selected arid zone trees and shrubs in Africa. FAO Conservation Guide 19, 52-58.
[5] Brink, M., 2006. Cordeauxia edulis Hemsl Record from Protobase. PROTA (Plant resources of tropical Africa / Ressourcesvégétales de l’Afriquetropicale), Wageningen, Netherlands http://database.prota.org/search.htm (Accessed on September 14, 2011)
[6] N.A.S (National Academy of Science), 1979. Tropical legumes: Resource for the Future, Nat Acad. Sci. Washington DC, 261 pp.
[7] FAO, 1988. Traditional food plants, Food and nutrition paper 42:224-27.
[8] Assefa, F., Bollini, R. and Kleiner, D., 1997. Agricultural potential of little used tropical legumes with special emphasis on Cordeauxia edulis (Ye-eb nut) and Sphenostylis stenocarpa (African yam bean). Giessener Beiträgezur Entwicklungsforschung, 24:237–242.
[9] Bally, P.R.O., 1966. Miscellaneous notes on the flora of Tropical East Africa, 29. Enquiry into the occurrence of the Yeheb nut (Cordeauxia edulis Hemsl.) in the Horn of Africa. Candollea 21 (1), 3-11.
[10] Hemming, C.F., 1972. The vegetation of the northern region of Somalia Republic. Proceeding of Linnaeus Social London, 177:173-250.
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[12] Mussa, M., 2010.Cordeauxia edulis (Yeheb): resource status, utilization and management in Ethiopia. Thesis for the degree of Philosophiae Doctor, University of Wales.
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[14] Al-Naggar, A.M.M., Shabana, R., Rady,M.R.,Ghanem, S.A., Saker, M.M., Reda, A.A., MatterM.A., and Eid, S.A.M., 2010. In vitro callus initiation and regeneration in some canola varieties. International Journal of Academic Research 2(6): 357-362
[15] Sié, R.S., Charles, G.,,Sakhanokho, H.F., Toueix, Y., Djè ,Y., Sangaré, A. and Branchard, M., 2010. Protocols for callus and somatic embryo initiation for Hibiscus sabdariffa L. (Malvaceae): Influence of explant type, sugar, and plant growth regulators. Australian Journal of Crop Science 4(2):98-106.
[16] SAS Institute Inc., 2002. Statistical Analysis Software, Version 9.0. Cary, North Carolina, USA.
[17] Rao, S., Patil, P. and Kaviraj, C.P., 2005. Callus induction and organogenesis from various explants in Vigna radiata (L.) Wilczek. Indian Journal of Biotechnology, 4:556-560.
[18] Amoo, S.O. and Ayisire, B.E., 2005. Induction of callus and somatic embryogenesis from cotyledon explants of Parkiabiglobosa (Jacq.) Benth.African Journal of Biotechnology, 4(1):68-71.
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Cite This Article
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    Yohannes Seyoum, Firew Mekbib, Adefris Teklewold, Belayneh Admassu, Dawit Beyene, et al. (2014). Induction of Callus and Somatic Embryogenesis from Cotyledon and Leaf Explants of Yeheb (Cordeauxia edulis Hemsl). Agriculture, Forestry and Fisheries, 3(6), 464-468. https://doi.org/10.11648/j.aff.20140306.15

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    Yohannes Seyoum; Firew Mekbib; Adefris Teklewold; Belayneh Admassu; Dawit Beyene, et al. Induction of Callus and Somatic Embryogenesis from Cotyledon and Leaf Explants of Yeheb (Cordeauxia edulis Hemsl). Agric. For. Fish. 2014, 3(6), 464-468. doi: 10.11648/j.aff.20140306.15

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    AMA Style

    Yohannes Seyoum, Firew Mekbib, Adefris Teklewold, Belayneh Admassu, Dawit Beyene, et al. Induction of Callus and Somatic Embryogenesis from Cotyledon and Leaf Explants of Yeheb (Cordeauxia edulis Hemsl). Agric For Fish. 2014;3(6):464-468. doi: 10.11648/j.aff.20140306.15

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  • @article{10.11648/j.aff.20140306.15,
      author = {Yohannes Seyoum and Firew Mekbib and Adefris Teklewold and Belayneh Admassu and Dawit Beyene and Zelalem Fisseha},
      title = {Induction of Callus and Somatic Embryogenesis from Cotyledon and Leaf Explants of Yeheb (Cordeauxia edulis Hemsl)},
      journal = {Agriculture, Forestry and Fisheries},
      volume = {3},
      number = {6},
      pages = {464-468},
      doi = {10.11648/j.aff.20140306.15},
      url = {https://doi.org/10.11648/j.aff.20140306.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aff.20140306.15},
      abstract = {‘Yeheb’ (Cordeauxia edulis Hemsl) is a multipurpose and evergreen shrub and endemic to southeastern corner of Ethiopia and Somalia. It is adapted to low and irregular rainfall and survives a very long dry season. It has enormous economic and food security roles to the pastoralist of Somali Region State in Ethiopia. However, the plant is threatened with extinction due to over exploitation and its’ poor natural regeneration capacity. The aim of this was to explore the potential for in vitro rapid regeneration of ‘yeheb’ from cotyledon and leaf explants on Murashige and Skoog (MS) media supplemented with 1.0 – 8.0 mg l-1concentrations of 2, 4-D for callus induction and 2.0 and 3.0 mg l-1concentration of N6-benzylaminopurine (BAP), thidiazuron (TDZ) and kinetin (Kin) with combination of 4.0 mg l-1 of 2, 4-Dichlorophenyl acetic acid (2, 4-D) for embryo induction. The result of these studies revealed that the highest percentage of callus induction (89%) were obtained from both leaf and cotyledon explants on MS media supplemented with 4.00 and 8.00 mg l-1 2, 4-D, respectively. The highest percentage of embryo regeneration responses (88.89 and 77.78%) were obtained from leaf and cotyledon explants on same media: MS media supplemented by 3.00 mg l-1 TDZ +4.00 mg l-1 2, 4-D. As a conclusion; this is the first attempt for callus and embryo in vitro regeneration of C. edulis and permissible result for mass propagation and cryopreservation.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - Induction of Callus and Somatic Embryogenesis from Cotyledon and Leaf Explants of Yeheb (Cordeauxia edulis Hemsl)
    AU  - Yohannes Seyoum
    AU  - Firew Mekbib
    AU  - Adefris Teklewold
    AU  - Belayneh Admassu
    AU  - Dawit Beyene
    AU  - Zelalem Fisseha
    Y1  - 2014/12/22
    PY  - 2014
    N1  - https://doi.org/10.11648/j.aff.20140306.15
    DO  - 10.11648/j.aff.20140306.15
    T2  - Agriculture, Forestry and Fisheries
    JF  - Agriculture, Forestry and Fisheries
    JO  - Agriculture, Forestry and Fisheries
    SP  - 464
    EP  - 468
    PB  - Science Publishing Group
    SN  - 2328-5648
    UR  - https://doi.org/10.11648/j.aff.20140306.15
    AB  - ‘Yeheb’ (Cordeauxia edulis Hemsl) is a multipurpose and evergreen shrub and endemic to southeastern corner of Ethiopia and Somalia. It is adapted to low and irregular rainfall and survives a very long dry season. It has enormous economic and food security roles to the pastoralist of Somali Region State in Ethiopia. However, the plant is threatened with extinction due to over exploitation and its’ poor natural regeneration capacity. The aim of this was to explore the potential for in vitro rapid regeneration of ‘yeheb’ from cotyledon and leaf explants on Murashige and Skoog (MS) media supplemented with 1.0 – 8.0 mg l-1concentrations of 2, 4-D for callus induction and 2.0 and 3.0 mg l-1concentration of N6-benzylaminopurine (BAP), thidiazuron (TDZ) and kinetin (Kin) with combination of 4.0 mg l-1 of 2, 4-Dichlorophenyl acetic acid (2, 4-D) for embryo induction. The result of these studies revealed that the highest percentage of callus induction (89%) were obtained from both leaf and cotyledon explants on MS media supplemented with 4.00 and 8.00 mg l-1 2, 4-D, respectively. The highest percentage of embryo regeneration responses (88.89 and 77.78%) were obtained from leaf and cotyledon explants on same media: MS media supplemented by 3.00 mg l-1 TDZ +4.00 mg l-1 2, 4-D. As a conclusion; this is the first attempt for callus and embryo in vitro regeneration of C. edulis and permissible result for mass propagation and cryopreservation.
    VL  - 3
    IS  - 6
    ER  - 

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Author Information
  • Dryland Crop Research Department, Somali Region Pastoral and Agro-pastoral Research Institute (SoRPARI), Jijiga, Ethiopia

  • School of Plant sciences, Haramaya University (HU), Dire-Dewa, Ethiopia

  • Crop Research Directorate, Ethiopian Institute of Agriculture Research (EIAR), Addis Ababa, Ethioipia

  • Holetta biotech Laboratory, Ethiopian Institute of Agriculture Research (EIAR), Holetta, Ethiopia

  • Holetta biotech Laboratory, Ethiopian Institute of Agriculture Research (EIAR), Holetta, Ethiopia

  • Dryland Crop Research Department, Somali Region Pastoral and Agro-pastoral Research Institute (SoRPARI), Jijiga, Ethiopia

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