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Depolymerized Chitosan: A Novel Milk Protein Stabilizer

Received: 26 September 2015     Accepted: 7 October 2015     Published: 20 October 2015
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Abstract

Chitosan is a bio-degradable polymer obtained easily from natural polymer chitin which is abundantly found in the outer hard skeleton of shellfishes like crab, lobster, and shrimp. It has immense pharmaceutical uses as anti-oxidant and anti-bacterial material. We have presented here one-step hydrothermal method of preparation of depolymerized water dispersible chitosan particles using citric acid as a depolymerizer and ionic cross linker and their stabilizing effect on cow milk proteins from fluorimetric and differential scanning calorimetric studies. We report for the first time protein stabilizing effect of depolymerized chitosan from thermal denaturation of milk protein. This has been explained by carbohydrate like structural skeleton of chitosan.

Published in Advances in Bioscience and Bioengineering (Volume 3, Issue 6)
DOI 10.11648/j.abb.20150306.11
Page(s) 56-58
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Chitosan, Milk Protein, Denaturation

References
[1] L. Hu, Y. Sun, Y. Wu, Advances in chitosan-based drug delivery vehicles, Nanoscale. 5(8) (2013) 3103-11.
[2] R. Susana, D. Marita, R. L. Carmen, G. Ana, Biocompatibility of Chitosan Carriers with Application in Drug Delivery, J. Funct. Biomater. 3 (2012) 615-41.
[3] M. Koping-Hoggard, I. Tubulekas, H. Guan, K. Edwards, M. Nilsson, K. M. Varum, P. Artursson, Chitosan as a nonviral gene delivery system. Structure-property relationships and characteristics compared with polyethylenimine in vitro and after lung administration in vivo, Gene therapy. 8(14) (2001) 1108-21.
[4] P. Erbacher, S. M. Zou, T. Bettinger, A. M. Steffan, J. S. Remy, Chitosan-based vector/DNA complexes for gene delivery: Biophysical characteristics and transfection ability, Pharmaceutical Research. 15(9) (1998) 1332-9.
[5] R. A. A. Muzzarelli, F. Greco, A. Busilacchi, V. Sollazzo, A. Gigante, Chitosan, hyaluronan and chondroitin sulfate in tissue engineering for cartilage regeneration: A review, Carbohydrate Polymers. 89 (3) (2012) 723-39.
[6] J. K. F. Suh, H. W. T. Matthew, Application of chitosan-based polysaccharide biomaterials in cartilage tissue engineering: a review, Biomaterials. 21(24) (2000) 2589-98.
[7] M. N. Kumar, R. A. A. Muzzarelli, C. Muzzarelli, H. Sashiwa, A. J. Domb, Chitosan Chemistry and Pharmaceutical Perspectives, Chemical Reviews. 104(12) (2004) 6017-84.
[8] L. Illum, Chitosan and its use as a pharmaceutical excipient, Pharmaceutical Research. 15(9) (1998) 1326-31.
[9] O. Felt, P. Buri, R. Gurny, Chitosan: A unique polysaccharide for drug delivery, Drug Development and Industrial Pharmacy. 24(11) (1998) 979-93.
[10] K. S. Nam, M. K. Kim, Y. H. Shon, Chemopreventive effect of chitosan oligosaccharide against colon carcinogenesis, Journal of Microbiology and Biotechnology. 17 (9) (2007) 1546-9.
[11] S. Hirano, M. Zhang, M. Nakagawa, T. Miyata, Wet spun chitosan-collagen fibers, their chemical N-modifications, and blood compatibility, Biomaterials. 21 (10) (2000) 997-1003.
[12] D. A. Patil, G. B. Patil, P. K. Deshmukh, V. S. Belgamwar, R. A. Fursule, Chitosan coated mucoadhesive multiparticulate drug delivery system for gliclazide, Asian Journal of Pharmaceutical and Clinical Research. 2(2) (2009) 62-8.
[13] Y. Okamoto, R. Yano, K. Miyatake, I. Tomohiro, Y. Shigemasa Y, S. Minami, Effects of chitin and chitosan on blood coagulation, Carbohydrate Polymers. 53 (3) (2003) 337-42.
[14] A. M. Qandil, A. A. Obaidat, M. A. M. Ali, B. M. Al-Taani, B. M. Tashtoush, N. D. Al-Jbour, M. M. Al Remawi, K. A. Al-Sou'od, A. A. Badwan, Investigation of the Interactions in Complexes of Low Molecular Weight Chitosan with Ibuprofen, Journal of Solution Chemistry. 38 (6) (2009) 695-712.
[15] M. Roldo, M. Hornof, P. Caliceti, A. Bernkop-Schnurch, Mucoadhesive thiolated chitosans as platforms for oral controlled drug delivery: synthesis and in vitro evaluation, European Journal of Pharmaceutics and Biopharmaceutics. 57 (1) (2004) 115-21.
[16] S. Basumallick, S. Santra, Chitosan coated copper-oxide nano particles: a novel electro-catalyst for CO2 reduction, RSC Advance. 4 (2014) 63685-90.
[17] D. G. Rao, Studies on Viscosity-Molecular Weight Relationship of Chitosan Solutions, Journal of Food Science and Technology (India). 30 (1) (1993) 66-7.
[18] A. Kulmyrzaev, E. Dufour, Determination of lactulose and furosine in milk using front-face fluorescence spectroscopy, Lait. 82 (2002) 725–35.
[19] V. Vagenende, M. G. S. Yap, B. L. Trout, Mechanisms of Protein Stabilization and Prevention of Protein Aggregation by Glycerol, Biochemistry. 48 (46) (2009) 11084–96.
[20] H. Hamada, T. Arakawa, K. Shiraki, Effect of Additives on Protein Aggregation, Current Pharmaceutical Biotechnology. 10 (2009) 400-7.
[21] A. Ben-Naim, Theory of cold denaturation of protein, Advance in Biological Chemistry. 3 (2013) 29-39.
[22] M. Ruegg, U. Moor, B. Blanc, A calorimetric study of the thermal denaturation of whey protein in stimulated milk ultrafiltrate, Journal of Diary Research. 44(3) (1977) 509-20.
[23] The nature of biological systems as revealed by thermal methods, Editor Denis Lorinczy, pp112-114, Springer publisher.
Cite This Article
  • APA Style

    Manna Majumder, Rupasree Chudhury, Tarun Kumar Mishra, Srijita Basumallick. (2015). Depolymerized Chitosan: A Novel Milk Protein Stabilizer. Advances in Bioscience and Bioengineering, 3(6), 56-58. https://doi.org/10.11648/j.abb.20150306.11

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    ACS Style

    Manna Majumder; Rupasree Chudhury; Tarun Kumar Mishra; Srijita Basumallick. Depolymerized Chitosan: A Novel Milk Protein Stabilizer. Adv. BioSci. Bioeng. 2015, 3(6), 56-58. doi: 10.11648/j.abb.20150306.11

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    AMA Style

    Manna Majumder, Rupasree Chudhury, Tarun Kumar Mishra, Srijita Basumallick. Depolymerized Chitosan: A Novel Milk Protein Stabilizer. Adv BioSci Bioeng. 2015;3(6):56-58. doi: 10.11648/j.abb.20150306.11

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  • @article{10.11648/j.abb.20150306.11,
      author = {Manna Majumder and Rupasree Chudhury and Tarun Kumar Mishra and Srijita Basumallick},
      title = {Depolymerized Chitosan: A Novel Milk Protein Stabilizer},
      journal = {Advances in Bioscience and Bioengineering},
      volume = {3},
      number = {6},
      pages = {56-58},
      doi = {10.11648/j.abb.20150306.11},
      url = {https://doi.org/10.11648/j.abb.20150306.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20150306.11},
      abstract = {Chitosan is a bio-degradable polymer obtained easily from natural polymer chitin which is abundantly found in the outer hard skeleton of shellfishes like crab, lobster, and shrimp. It has immense pharmaceutical uses as anti-oxidant and anti-bacterial material. We have presented here one-step hydrothermal method of preparation of depolymerized water dispersible chitosan particles using citric acid as a depolymerizer and ionic cross linker and their stabilizing effect on cow milk proteins from fluorimetric and differential scanning calorimetric studies. We report for the first time protein stabilizing effect of depolymerized chitosan from thermal denaturation of milk protein. This has been explained by carbohydrate like structural skeleton of chitosan.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Depolymerized Chitosan: A Novel Milk Protein Stabilizer
    AU  - Manna Majumder
    AU  - Rupasree Chudhury
    AU  - Tarun Kumar Mishra
    AU  - Srijita Basumallick
    Y1  - 2015/10/20
    PY  - 2015
    N1  - https://doi.org/10.11648/j.abb.20150306.11
    DO  - 10.11648/j.abb.20150306.11
    T2  - Advances in Bioscience and Bioengineering
    JF  - Advances in Bioscience and Bioengineering
    JO  - Advances in Bioscience and Bioengineering
    SP  - 56
    EP  - 58
    PB  - Science Publishing Group
    SN  - 2330-4162
    UR  - https://doi.org/10.11648/j.abb.20150306.11
    AB  - Chitosan is a bio-degradable polymer obtained easily from natural polymer chitin which is abundantly found in the outer hard skeleton of shellfishes like crab, lobster, and shrimp. It has immense pharmaceutical uses as anti-oxidant and anti-bacterial material. We have presented here one-step hydrothermal method of preparation of depolymerized water dispersible chitosan particles using citric acid as a depolymerizer and ionic cross linker and their stabilizing effect on cow milk proteins from fluorimetric and differential scanning calorimetric studies. We report for the first time protein stabilizing effect of depolymerized chitosan from thermal denaturation of milk protein. This has been explained by carbohydrate like structural skeleton of chitosan.
    VL  - 3
    IS  - 6
    ER  - 

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Author Information
  • Department of Chemistry, National Institute of Technology, Agartala, India

  • Department of Chemistry, National Institute of Technology, Agartala, India

  • Department of Chemistry, National Institute of Technology, Agartala, India

  • Department of Chemistry, National Institute of Technology, Agartala, India

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