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High Prevalence of Virulence Genes and in Vitro Biofilm Production in Clinical Multidrug-Resistant Escherichia coli in Dakar Senegal

Bacterial virulence is a key factor determining the outcome of each bacterial infection, and virulent bacteria are often associated with high-risk infections. Thus, this study aimed to screen for virulence genes and evaluate the in vitro biofilm formation capacity of multidrug-resistant Escherichia coli isolated in Dakar. For the 16 virulence genes identified by standard polymerase chain reaction (PCR), all 78 ExPEC isolates carried at least four virulence genes. The prevalence of virulence genes was as follows: adhesin genes fimH (98.7%), mrkD (98.7%), papC (46.2%), afaC (9%), and sfa/focDE (1.3%); iron acquisition system genes entB (98.7%), fepA (98.7%), ybtS (93.6%), fyuA (91%), iucA (91%), iucB (91%), iutA (34.6), iroB (6.4%), iroN (6.4%), and toxin genes hlyA (10.3%) and cnf (1 & 2) (10.3%). Seventy-five of the 78 isolates (96.2%) carried at least two adhesin genes and two iron capture system genes. Evaluation of the biofilm formation capacity revealed that all (29/29) hospital-acquired isolates were biofilm producers with (6/29; 20.7%) strong biofilm producers, (15/29; 51.7%) moderate biofilm producers and (8/29; 27.6%) weak biofilm producers. Hospital-acquired isolates carrying papC had a greater biofilm formation capacity than those lacking papC (p < 0.001). The deepening of this type of study on bacterial virulence and hospital bacterial biofilms could lead to improvements in infection investigation, prevention, and therapeutic protocols.

Virulence Genes, Virulence Factors, Extraintestinal Pathogenic Escherichia coli, Biofilms, Biofilm-Associated Infections

APA Style

Komla Mawunyo Dossouvi, Bissoume Sambe Ba, Gora Lo, Issa Ndiaye, Awa Ba-Diallo, et al. (2023). High Prevalence of Virulence Genes and in Vitro Biofilm Production in Clinical Multidrug-Resistant Escherichia coli in Dakar Senegal. International Journal of Microbiology and Biotechnology, 8(4), 69-81.

ACS Style

Komla Mawunyo Dossouvi; Bissoume Sambe Ba; Gora Lo; Issa Ndiaye; Awa Ba-Diallo, et al. High Prevalence of Virulence Genes and in Vitro Biofilm Production in Clinical Multidrug-Resistant Escherichia coli in Dakar Senegal. Int. J. Microbiol. Biotechnol. 2023, 8(4), 69-81. doi: 10.11648/j.ijmb.20230804.11

AMA Style

Komla Mawunyo Dossouvi, Bissoume Sambe Ba, Gora Lo, Issa Ndiaye, Awa Ba-Diallo, et al. High Prevalence of Virulence Genes and in Vitro Biofilm Production in Clinical Multidrug-Resistant Escherichia coli in Dakar Senegal. Int J Microbiol Biotechnol. 2023;8(4):69-81. doi: 10.11648/j.ijmb.20230804.11

Copyright © 2023 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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