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Marek’s Disease in Broiler Farms, Iran, 2021: The Phylogenetic Study

Marek's disease virus (MDV) is a highly cell-associated oncogenic alphaherpesvirus that causes chicken T-cell lymphoma. MD is currently controlled by vaccination; however, MDV strains tend to develop increased virulence. Distinct diversity and point mutations are present in the Meq proteins, the major oncoproteins of MDV, suggesting that changes in protein function induced by amino acid substitutions might affect MDV virulence. This study sampled 30 commercial broiler flocks from different Provinces (10 spleens from each flock) at slaughter. Gallid alphaherpesvirus 2 was identified in PCR (using gb primer) of spleen samples of 11 flocks (36.67%). Two provinces, Azerbaijan Gharbi (44%) and Golestan (25%), recorded the highest and lowest infection rates. The oncogene Meq of some positive samples was amplified by PCR and sequenced. MDV strains detected in this study could be put in three branches, with molecular features consistent with virulent and very virulent previously identified MDV. UT-PCR9303 was located with an Iraqi isolate. UT-PCR-9231 had high homology with an Iranian MDV sequence detected from a layer farm with MD. UT-PCR-9380 was located with vv MDV from Japan and Colombia. Therefore, the relatively high rate of Meq in the unvaccinated broiler farms constitutes support for vaccination. These findings provide the basis for molecular surveillance and further study of MDV mutants and strategies for managing MD in Iran.

Marek Disease, Iran, Broiler, Phylogenetic Analysis, Meq

APA Style

Rima Morshed, Hossein Hosseini, Naser Sadri, Zahra Ziafati Kafi, Soroush Sarmadi, et al. (2023). Marek’s Disease in Broiler Farms, Iran, 2021: The Phylogenetic Study. International Journal of Microbiology and Biotechnology, 8(3), 48-53.

ACS Style

Rima Morshed; Hossein Hosseini; Naser Sadri; Zahra Ziafati Kafi; Soroush Sarmadi, et al. Marek’s Disease in Broiler Farms, Iran, 2021: The Phylogenetic Study. Int. J. Microbiol. Biotechnol. 2023, 8(3), 48-53. doi: 10.11648/j.ijmb.20230803.11

AMA Style

Rima Morshed, Hossein Hosseini, Naser Sadri, Zahra Ziafati Kafi, Soroush Sarmadi, et al. Marek’s Disease in Broiler Farms, Iran, 2021: The Phylogenetic Study. Int J Microbiol Biotechnol. 2023;8(3):48-53. doi: 10.11648/j.ijmb.20230803.11

Copyright © 2023 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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