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Effect of Cooking Time on Biochemical Parameters and Some Functional Properties of Lima Bean Seed Flours (White, Red and Black) Consumed in Côte d'Ivoire

Meeting the world's food needs requires efforts to promote the cultivation and use of underutilized and neglected plants, which have the potential to improve food and nutritional security. Phaseolus lunatus (L.), is one of them can serve as a promising potential food crop. This study aims to contribute to food security throught he valorization of P. lunatus (L.) beans seeds, with a view to their more rational use indifferent diets. Seeds obtained at stage 4 (52days) of maturity after harvesting were cooked for 45, 60 and 75 minutes at 100°C. The results showed an increase in moisture content (29.68 to 36,27%), carbohydrates (64.16 to 71.56%) and fiber (4.62 to 6.05%), followed by a reduction in protein (4.62 to 6.05%), ash (4.62 to 6.05%) and lipids (4.62 to 6.05%). The results also showed a non-significant decrease in essential and non-essential aminoacids during cooking. Similarly, cooking increased water absorption capacity (WAC) (230.00 to 322.60%), oil absorption capacity (OAC) (190.36 to 250.36%), swelling power (SP) (2.26 to 23.33gwater/gDM) and solubility (1.80 to19.90%) at temperatures ranging from 50 to 90°C. The study provides useful information for consumers and food manufacturers that lima bean flour has great potential to increase the nutritional value of foods.

Cooking, Neglected Plants, Underutilized, Food Security, Food Manufacturers

APA Style

Tchumou Messou, Oupoh Bada Bedos, Dembele Syndoux, Tano Kablan. (2023). Effect of Cooking Time on Biochemical Parameters and Some Functional Properties of Lima Bean Seed Flours (White, Red and Black) Consumed in Côte d'Ivoire. American Journal of BioScience, 11(5), 111-121.

ACS Style

Tchumou Messou; Oupoh Bada Bedos; Dembele Syndoux; Tano Kablan. Effect of Cooking Time on Biochemical Parameters and Some Functional Properties of Lima Bean Seed Flours (White, Red and Black) Consumed in Côte d'Ivoire. Am. J. BioScience 2023, 11(5), 111-121. doi: 10.11648/j.ajbio.20231105.11

AMA Style

Tchumou Messou, Oupoh Bada Bedos, Dembele Syndoux, Tano Kablan. Effect of Cooking Time on Biochemical Parameters and Some Functional Properties of Lima Bean Seed Flours (White, Red and Black) Consumed in Côte d'Ivoire. Am J BioScience. 2023;11(5):111-121. doi: 10.11648/j.ajbio.20231105.11

Copyright © 2023 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

1. Amini. R., Pezhgan. h. and Mohammadinasab. A. D. (2013). Effect of weeds compétition on some growth parameters of red, white and pinto bean (Phaseolus vulgaris L.). Journal of Biodiversity and Environnemental Sciences (JBES). 3.86-93.
2. Corte's AJ, Monserrate FA, Ram1-'rez-Villegas J, Madrin- a'n S, Blair MW. (2013). Drought Tolerance in Wild Plant Populations: The Case of Cornmon Beans (Phaseolus vulgaris L.). PLoS ONE 8 (4): e62898. doi: l0.1371/journal.pone.0062898.
3. Sparvoli F, Laureati M, Pilu R, Pagliarini E, Toschi 1, Giuberti G, Fortunati P, Daminati MG, Cominelli E and Bollini R. (2016). Exploitation of Common Bean Flours with Low Antinutrient Content for Making Nutritionally Enhanced Biscuits. Front. Plant Sei. 7: 928.
4. Kizito Iheanacho. M. E. (2010). Comparative studies of the nutritional composition of soybean (Glycine max) and Lima bean (Phaseolus lunatus). Scientia Africana, 9: 29-35.
5. Ezeagu E. I. & Ibegbu M. D. (2010). Biochemical composition and nutritional potential of ukpa: a variety of tropical Lima beans (Phaseolus lunatus) from Nigeria. Polish Journal Food Nutrition Sciences, 60 (3): 231-235.
6. Gardner C. D. I., Chatterjee C., Rigby A., Spiller G. & Farquhar J. W. (2005). Effects of a plant- based diet on plasma lipids in hypercholesterolemic Adults. Annals International Med, 142: 725-1155.
7. Andriamamonjy N. (2000). Valeur nutritionnelle des graines sèches de 7 variétés de haricot et de 2 variétés d’Ambérique (Mémoire de DEA de Biochimie appliquée aux sciences de l’alimentation et à la nutrition). Faculté des sciences: Université d’Antananarivo, 53p.
8. Tchumou. (2017): Ethnobotanical survey and physicochemical characterization of bean seeds, Phaseolus lunatus (Fabaceae) consumed in the South and East of Côte d'Ivoire according to maturity level and cooking time.
9. Yellavila, S. B., J. K. Agbenorhevi, J. Y. Asibuo and G. O. Sampson. (2015). Proximate composition, minerals content and functional properties of five lima bean accessions. J. Food Secur. 3: 69-74.
10. AOAC. (1990). Official methods of analysis of the Association of Official Analytical Chemists, 15th ed, Washington DC, 1230p.
11. Bernfeld. (1955). Amylase β and α, In: method in enzymology 1, Colowick S. P. and Kaplan N. O., Academic Press, pp149-154.
12. FAO/INFOODS. (2015). FAO/INFOODS Guidelines for verifying food composition data before publication of a user table/database-Version 1.0. FAO, Rome.
13. Fatima Ismail., Farah N., Talpur. & Memon A. N. (2013). Determination of Water Soluble Vitamin in Fruits and Vegetables Marketed in Sindh Pakistan. Pakistan Journal Nutrition, 12: 197-199.
14. Oshodi A. A. (1992). Proximate composition, nutritionally valuable minerals and functional properties of Adenopus breviflorus benth seed flour and protein concentrate. Food.
15. Phillips R. D., Chinnan M. S., Branch A. L., Miller J. & Mcwatters K. H. (1988). Effects of pre-treatment on functional and nutritional properties of cowpea meal. Journal of Food Sciences, 53: 805-809.
16. Eke O. S. & Akobundu E. N. T. (1993). Functional properties of African yam bean (Sphenostylis stenocarpa) seed flour as affected by processing. Food Chemical, 48: 337-340.
17. Adebooye O. C. & Singh V. (2008). Physico-chemical properties of the flours and starches of two cowpea varieties (Vigna unguiculata L.) Walp). Innovative Food Science and Emerging Technologies, 9: 92-100.
18. Rehman A. R. & I. A. Khalil. (1988). Retention of selected nutrients in cooked kidney beans (Phaseolus vulgaris. L.). Journal of Agriculture of Food Chemistry, 32: 342-346.
19. Bjorck I., ASP N. G., Birkhe D. & Lunquist I. (1984). Effects of processing on avaibility of starch digestion in vitro and in vivo. Extrusion cooking of wheat flours and starch. Journal of Cereal Science, 2: 91-103.
20. Alajaji S. A. & El-Adawy T. A. (2006). Nutritional composition of chickpea (Cicer arietinum L.) as affected by microwave cooking and other traditional cooking methods. Journal of Food Composition and Analysis, 19: 806-812.
21. Gupta V. & Nagar R. (2008). Physico-chemical and acceptability of rabadi (a fermented soya flour product) as affected by cooking and fermentation time. International Journal of Food Science and Technology, 43: 939-943.
22. Abeke F. O., Ogundipe S. O., Sokoni A. A., Dafwang I. I., Adeyinka I. A., Oni O. O. & Abeke A. (2007). Growth and subsequent egg production performance of shika-brown pullets fed graded levels of cooked lablab purpureus beans. Pakistan Journal of Biological Sciences, 10: 1051-1061.
23. Ocheme O. B., Oludamilola O. O. & Gladys M. E. (2010). Effect of lime soaking and cooking (nixtamalization) on the proximate functional and some anti-nutritional properties of millet flour. Assumption University Journal of Technology, 14: 131- 138.
24. FAO / WHO (Food & Agriculture Organization & the Word Health Organization). (2007). Protein and amino acid requirements in human nutrition. Peport of a Joint WHO / FAO /UNU Expert Technical Report Series 935. Cholé-Doc N°111.
25. Okaka J. C., Akobundu E. N. T. & Okaka A. N. C. (1992). Human Nutrition – An Intergrated Approach. Obio Press Ltd., Enugu, Pp. 182-220.
26. Vadivel V. & Pugalenthi M. (2009). Effect of soaking in sodium bicarbonate solution followed by autoclaving on the nutritional and antinutritional properties of velvet bean seeds. Journal Food Process Preservation, 33: 60-73.
27. Nzewi D. & Egbuonu A. C. C. (2011). Effect of boiling and roasting on the proximate properties of asparagus bean (Vigna Sesquipedalis). African Journal of Biotechnology, 10: 11239-11244.
28. Aletor V. A. (1993). Allelochemicals in plant foods and feeding Stuffs. Part I. Nutritional, Biochemical and Physiopathological aspects in animal production. Vetenary and Human Toxicology, 35: 57-67.
29. Bressani T. (1993). Grain quality of common beans. Food Review International 9: 237–297.
30. Slavin J. L. (1987). Dietary fiber, and body weight. Journal of American Dietetic Association, 87: 1164-1168.
31. Lintas C &. Cappelloni M. (1998). Dietary fiber, resistant starch and in vitro starch digestibility of cereal meals. Food Science and Nutrition, 42: 117-124.
32. FAO. (2001). La nutrition dans les pays en voie de développement. FAO Ed, Genève, 515 pp.
33. Ezeocha V. C. & Ojimelukwe P. C. (2012). The impact of cooking on the proximate composition and anti-nutritional factors of water yam (Dioscorea alata). Journal of Stored Products and Postharvest Research, 3: 172 - 176.
34. Butte N. F. (1996). Energy requirements of infants. European Journal of Clinical Nutrition 50: 24-36.
35. Tran G. & Skiba F. (2005). Variabilité inter et intra matière première de la teneur en phosphore total et phytique et de l’activité phytasique. INRA Productions Animales, 18: 159- 168.
36. Brune M., Hallberg L. & Skanberg A. B. (1991). Determination of iron-binding phenolic groups in foods. Journal of Food Science, 56: 131-137.
37. Alonso R., Rubio L. A., Muzquiz M. & Marzo F. (2001). The effect of extrusion cooking on mineral bioavailability in pea and kidney bean seed meals. Animal Feed Sciences and Technology, 94: 1-13.
38. Anigo I. A., Ameh D. A., Ibrahim S. & Danbauchi S. S. (2009). Nutrient composition of commonly used complimentary foods in north western Nigeria. African Journal of Biotechnology 8: 4211- 4216.
39. Ewald C., Fjelkner-Modig S., Johansson K., Sjoholm I. & Akesson B. (1999). Effect of processing on major flavonoids in processed onions, green beans, and peas. Food Chemistry, 64: 231-235.
40. Nieman D. C., Butterworth. & Nieman C. N. (1992). Nutrition, WmC. Brown publishers. Dubugue, USA, 237-312pp.
41. Siddhuraju P. & Becker K. (2001). Effect of various domestic processing methods on antinutrients and in vitro-protein and starch digestibility of two indigenous varieties of Indian pulses, Mucuna pruries var utilis. Journal Agriculture of Food Chemistry, (49): 3058-3067.
42. Hefnawy. T. H. (2011). Effect of processing methods on nutritional composition and antinutritional factors in lentils (Lens culinaris), 56: 57-61.
43. Khalil A. H. & Mansour E. H. (1995). The effect of cooking, autoclaving and germination on the nutritional quality of faba beans. Food Chemistry, 54: 177–182.
44. Fagbemi T. N. & Olaofe O. (2000). The chemical composition and functional properties of raw and precooked Taro (Cococasia esculenta) flour. Journal of Biology Physical and Science, 1: 98-103.
45. Abulude F. O. (2004). Effects of processing on nutritional composition, phytate and functional properties of rice (Oryza sativa) flour. Nigerian Food Journal, 22: 97-104.
46. Dengate H. N. (1984). Swelling, pasting and gellin of wheat starch, in Advances in Cereal and Technology (eds). By Pomeranz Y. AACC Eagan Press, St Paul, MN, 2: 49-84.
47. Olaofe O., Adeyemi F. O. & Adediran G. O. (1994). Amino acid and mineral and functional properties of some Oilseeds. Journal of Agricultural and Food Chemistry, 42: 878-881.
48. Wolf W. J. (1970). Soybean proteins: their functional, chemical and physical properties. Journal of Agriculture and Food Chemistry, 18: 965-969.
49. Mbofung C. M. F., Aboubakar N. Y., Njintang A., Bouba A. & Balaam F. (2006). Physicochemical and functional properties of six varieties of taro (Colocasia esculenta L.) flours. Journal of Food Technology, 4: 135-142.
50. Altan A., McCarthy K. L. & Maskan M. (2009). Effect of extrusion coking on functional properties and in vitro starch digestibility of barley-based extrudates from fruit and vegetable by-products. Journal of Food Science, 74: 77- 86.
51. Hsu C. L., Chen W., Weng Y. M. & Tseng C. Y. (2003). Chemical composition, physical properties, and antioxidant activities of yam flours as affected by different drying methods. Food Chemistry, 83: 85-92.
52. Aremu M. O., Olonisakin A; Atolaye B. O. & Ogbu C. F. (2006). Some nutritional and functional studies of Prosopis africana. Electronic Journal of Environmental, Agricultural and Food Chemistry, 5: 1640-1648.
53. Ubbor S. C. & Akobundu E. N. T. (2009). Quality Characteristics of Cookies from Composite Flours of Watermelon Seed, Cassava and Wheat. Pakistan Journal of Nutrition, 8: 1097-1102.
54. El-Adawy T. A. (2000). Functional properties and nutritional quality of acetylated succinylated mung bean protein isolate. Food Chemisty, 70: 83-91.
55. Moure A., Sineiro J., Dominguez H. & Parajo J. C. (2006). Fonctionality of oil seed protein product: A review. Food Resaerch International, 38: 945-963.
56. Odoemelam S. A. (2005). Functional properties of raw and heat processed jackfruit (Artocarpus heterophyllus) flour. Pakistan Journal of Nutrition, 4: 366-370.
57. Ukpabi U. J. (2010). Farmstead bread making potential of lesser yam (Dioscorea esculenta) flour in Nigeria. Australian Journal of Crop Science, 4: 68-73.
58. James P. C. (1980). Pulp and Paper Chemistry and Chemical Technology, John Wiley, New York, 3ème edition, 3: 1475-1508.
59. Ogawa Y., Glenn G. M., Orts W. J. & Wood D. F. (2003). Histological structures of cooked rice grain. Journal of Agriculture and Food Chemistry, 51: 7019-7023.
60. Okezie B. O. & Bello A. B. (1988). Physicochemical and functional properties of winged bean flours and isolate compared with soy isolate. Journal of Food Science, 53: 445 - 450.
61. Yu S., Ying M., Menager L. & Da-Wen S. (2012). Physicochemical Properties of Starch and Flour from Different Rice Cultivars. Food Bioprocess Technology, 5: 626-637.
62. Loos P. J., Hood L. F. & Graham H. D. (1981). Isolation and characterization of starch from breadfruit. Cereal Chemistry, 58: 282-286.
63. Baafi E. & Safo-Kantanka O. (2007). Effect of genotype, age and location on cassava flour yield and quality. Journal of Plant Science, 2: 607-612.