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Factors Responsible for the Success of Anther Culture in Pepper Plant: A Review

Following the first successful plant regeneration from pepper in 1973, numerous research projects have been carried out to determine the factors influencing the induction of pepper plant regeneration and for the optimization of an anther culture in pepper. However, a variety of factors, are responsible for successful production of embryos. Critical factors for embryo production and plantlet regeneration are growing condition, donor plants growth conditions, selection of flower buds with microspores at the proper time and stage, and etc. This paper reviewed findings on three important factors responsible for the success of anther culture which is valuable for future research works and to understand factors in the development of haploid plants aimed at accelerating the breeding programs of pepper plants. One of the factors influencing the growth of anther culture is the effects of the growing season. The highest embryogenic yields were recorded with a genotype of growing in the summer season than that of winter season. The genotype of the donor plant is the second most crucial factor affecting androgenic potential, and it is essential to know the developmental time of pollen that can enhance anther culture efficiency. The breeding process for the development of plant varieties with high yield, disease resistance, and better quality attributes can be sped up by using a highly reproducible anther culture technique with optimized culture variables. In addition to the aforementioned variables, other factors would be taken into consideration for the effective development of another culture in pepper.

Anther Culture, Donor Plant Age, Growth Conditions, Pepper

APA Style

Ousman Yimer, Mohammed Abu. (2023). Factors Responsible for the Success of Anther Culture in Pepper Plant: A Review. Advances in Bioscience and Bioengineering, 11(3), 72-75.

ACS Style

Ousman Yimer; Mohammed Abu. Factors Responsible for the Success of Anther Culture in Pepper Plant: A Review. Adv. BioSci. Bioeng. 2023, 11(3), 72-75. doi: 10.11648/

AMA Style

Ousman Yimer, Mohammed Abu. Factors Responsible for the Success of Anther Culture in Pepper Plant: A Review. Adv BioSci Bioeng. 2023;11(3):72-75. doi: 10.11648/

Copyright © 2023 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

1. Abak, K. (1983). Study on the anther culture in vitro of pepper (Capsicum annuum). Capsicum Newslett. 2, 66–67. annuum L.) breeding. Acta Agron Hung 58(3): 259–266.
2. Arnedo Andres M. S, Garcel Claver A, Esteban Chapapria J, Peiro Abris JL, Palazon C, Luis Arteaga M and Gil Ortega R. (2004). Application of anther culture and molecular markers to a pepper breeding program for diseases resistance. Capsicum Eggplant Newsl 23: 105–108.
3. Barbary A, Palloix A, Fazari A, Marteu N, Castagnone-Sereno P, Djian-Caporalino C. (2014). The plant genetic background affects the efficiency of the pepper major nematode resistance genes Me1 and Me3. Theor Appl Genet 127: 499-507.
4. Başay, S., & Ellialtioglu, S. S. (2013). Effect of genotypical factors on the effectiveness of anther culture in eggplant (Solanum melongena L.). Turkish Journal of Biology. 37: 499-505.
5. Ciner, D. O. and Tipirdamaz, R. (2002). The effects of cold treatment and charcoal on the in vitro androgenesis of pepper (Capsicum annuum L.). Turkish Journal of Botany, 26(3), pp. 131-139.
6. Dumas de Vaulx R. (1990). Haploid and pepper breeding: a review. Capsicum Newsl 8–9: 13–17. eggplant, Antalya, Turkey, pp 142–145.
7. Ellialtioglu S, Kaplan E. and Abak K. (2001). The effect of carrot extract and activated charcoal on the androgenesis of pepper. In: XIth EUCARPIA meeting on genetics and breeding of capsicum and Carbohydrate source and antioxidants. Biotech Studies, 30(2), pp. 92-97.
8. Ercan, N., Sensoy, F. A. and Sensoy, A. S. (2006). Influence of growing season and donor plant age on anther culture response of some pepper cultivars (Capsicum annuum L.). Scientia horticulturae, 110(1), pp. 16-20.
9. Gemesne Juhász A, Petus M, Venczel G, Zatykó L, Gyulai G, Cséplö M. (2001). Genetic variability of anther donor versus spontaneous doubled haploid descendants and colchicines induced doubled haploid sweet pepper (Capsicum annuum L.) lines. Acta Hortic 560: 149-152.
10. George, L. and Narayanaswamy, S. (1973). Haploid Capsicum through experimental androgenesis. Protoplasma, 78(4), 467-470.
11. Guha, S. and Maheshwari, S. C. (1964). In vitro production of embryos from anthers of Datura. Nature, Vol. 204, No. 4957, (October 1964), pp. 497, ISSN 0028-0836.
12. Gyulai G, Gemesne Juhasz A, Sagi Z, Zatyko L, Heszky L. and Venczel G. (2000). Doubled haploid development and PCR-analysis of F hybrid derived DH—R2 paprika (Capsicum annuum L.) lines. J Plant Physiol 156: 168–174.
13. Hayati, B. A. T., Altindag, F. N., Yigit, M. A., Ellialtioglu, S. S. and Cmlekcioglu, N. (2022). Genotype Effect as one of the Affecting Factors on the Success of Anther Culture in Eggplant (Solanum melongena L.). Horticultural Studies, pp. 41-47.
14. Irikova, T., Grozeva, S. and Rodeva, V. (2011). Anther culture in pepper (Capsicum annuum L.) in vitro. Acta physiologiae plantarum, 33, pp. 1559-1570.
15. Kristiansen, K. and Andersen, S. B. (1993). Effects of donor plant temperature, photoperiod, and age on anther culture response of Capsicum annuum L. Euphytica, 67, pp. 105-109.
16. Mangal, M. and Srivasatava, A., (2019). Exploitation of morphological features of bud and anther development for prediction of stages of microsporogenesis and microgametogenesis in pepper.
17. Nervo G, Azzimonti MT, Bonelly A, and Tamietti G. (2007). Application of in vitro anther culture methods to a pepper breeding program for disease resistance. In: Proceeding of the 51st Italian Society of Agricultural Genetics Annual Congress Riva del Granda, Italy 23–26 September, Poster Abstract S 2. 03.
18. Niklas-Nowak, A., Olszewska, D., Kisiała, A. and Nowaczyk, P. (2012). Study of individual plant responsiveness in anther cultures of selected pepper (spp.) genotypes. Folia Horticulturae, 24(2), pp. 141-146.
19. Novak F. (1974). Induction of a haploid callus in anther cultures of Capsicum sp. Z Pflanzenzücht 72: 46-54.
20. Nowaczyk, P., Olszewska, D. and Kisiała, A. (2009). Individual reaction of Capsicum F 2 hybrid genotypes in anther cultures. Euphytica, 168, pp. 225-233.
21. Ouyang, J. W., He, D. G., Feng, G. H. and Jia, S. E. (1987). The response of anther culture to culture temperature varies with growth conditions of anther-donor plants. Plant Sci. 49, 145–148.
22. Pauk J, Lantos C, Somogyi G, Vagi P, Abraham TZ, Gemes JA, ihaly R, Kristof Z, Somogyi N, and Timar Z. (2010). Tradition, quality and biotechnology in Hungarian spice pepper (Capsicum annuum L.) breeding. Acta Agron Hung 58(3): 259–266.
23. Segui-Simarro, J. M. and Nuez, F. (2008). How microspores transform into haploid embryos: changes associated with embryogenesis induction and microspore derived embryogenesis. Physiologia Plantarum, 134(1), 1–12.
24. Shariatpanahi, M. E., Bal, U., Heberle-Bors E. and Touraev, A. (2006). Stresses applied for the re-programming of plant microspores towards in vitro embryogenesis. Physiologia Plantarum, 127(4), 519–534.
25. Sibi M, Dumas de Vaulx R. and Chambonnet D. (1979). Obtaining haploid plants by in vitro androgenesis in red pepper (Capsicum annuum L.). Annales de l’Amelioration des Plantes 29: 583–606.
26. Takahata, Y., Brown, D. C. W., & Keller, W. A. (1991). Effect of donor plant age and inflorescence age on microspore culture of Brassica napus L. Euphytica, 58, 51-55.
27. Vagera, J. (1990). Pepper (Capsicum spp.): in vitro induction of haploids. Biotechnol. Agric. For. 12, 374–392.
28. Wang, Y. Y., Sun, C. S., Wang, C. C., and Chien, N. F. (1973). The induction of the pollen plantlets of triticale and Capsicum annuum L. from anther culture. Science Sinica, 16, 147-151.