In Vitro antisickling and Free Radical Scavenging Activities of Kigelia africana (LAM) Stem Bark
Tatiana Kangah Mireille Kple,
Joel Akakpo-Akue,
Koffi Adou Mathieu Kra,
N’Guessan Bra Yvette Fofie,
Koffi Julien Golly,
Ibrahime Sanogo,
Antoinette Claire Chiaye Yapo-Crezoit
Issue:
Volume 7, Issue 1, March 2021
Pages:
1-5
Received:
12 December 2020
Accepted:
30 December 2020
Published:
30 January 2021
Abstract: Kigelia africana dried bark has been recommended in the management of sickle cell anemia by traditional practitioners. The aim of this study was to evaluate in vitro antioxidant and sickling inhibitory activities of Kigelia africana. Quantitative estimation of phenolic compounds was performed using colorimetric method in hydro-ethanolic extract (70%) and decocted. The sickling inhibitory activity was determinate according to Emmel method and the evaluation of antioxidant properties was carried out using the method of scavenging the free radical DPPH. The obtained results indicated that Kigelia africana species contains flavonoids (8.61±1.08 mg QE and 9.78±1.14 mg QE/g of dry weight) and total phenols (9.48±0.19b mg GAE/g and 11.11±0.22 mg GAE /g of dry weight) in decocted and hydroethanolic extract respectively. The IC50 values of the ethanolic extract and the decocted were 0.320±0.01 and 0.468±0.04 mg/mL respectively. The in vitro sickle-formation inhibition test indicated the value of 89% and 82.36% for the ethanolic extract and the decocted at 10mg/mL respectively. Those values were higher than 80% which was the phenylalanine value. Both extracts showed antioxidant and sickling inhibitory activities. Overall, there could have a correlation between these activities and phenolic compound content in this studied plant extracts. These results would justify the use of this plant in rural environment.
Abstract: Kigelia africana dried bark has been recommended in the management of sickle cell anemia by traditional practitioners. The aim of this study was to evaluate in vitro antioxidant and sickling inhibitory activities of Kigelia africana. Quantitative estimation of phenolic compounds was performed using colorimetric method in hydro-ethanolic extract (70...
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Traditional Healing Methods: Focus on the Medicinal Plants Against Coronavirus (COVID-19) Infection
Yusuf-Babatunde Moruf Ademola,
Quadri Jelili Akorede,
Solaja Olatunde Odutola,
Kuku Mukaila Akorede
Issue:
Volume 7, Issue 1, March 2021
Pages:
6-13
Received:
23 December 2020
Accepted:
8 January 2021
Published:
9 February 2021
Abstract: Background: In African health care system, medicinal plants are major components and most assorted of all therapeutic systems. In major parts of rural Africa, traditional healers prescribing medicinal plants are the most easily accessible and affordable health resource available to the local community and at times the only therapy that subsists. Coronavirus diseases (COVID-19) is an acute virus (SARs-coV-2) which has caused a global pandemic. This paper aims to emphasize the importance of herbal medicine as a possible alternative and effective immune system booster against coronavirus disease in Ogun state, Nigeria. Materials and Methods: Ethnobotanical survey was carried out using 80 randomly selected respondents among the villagers, herbalists, herb sellers, traditional medical practitioners, students, community leaders, by oral interviews and semi-structured questionnaire. The data were analyzed using frequency counts and percentages. Results: A total of 81 plant species from 35 families which were mostly represented by Euphorbiaceae, Moraceae, Apocynaceae and Rubiaceae, and 61 botanicals were used singly while 21 other botanicals were combination of two or more plants in a single herbal preparations reported in the study area are used for management and alternative therapy against ailments such as fever, cough, body pain, flu, cold and shortness of breath that are associated to symptoms of COVID-19. Conclusion: Herbal medicines provide an alternative therapy for boosting immunity system to help fight coronavirus. Traditional healers are still very relevant to African healthcare system.
Abstract: Background: In African health care system, medicinal plants are major components and most assorted of all therapeutic systems. In major parts of rural Africa, traditional healers prescribing medicinal plants are the most easily accessible and affordable health resource available to the local community and at times the only therapy that subsists. Co...
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Chemical Characterization and Antibacterial Efficacy of Essential Oils of Three Lamiaceae Species Growing in Cameroon
Helene Gueaba Mbuntcha,
Hervet Paulin Dogmo Fogang,
Verlaine Woguem,
Patrick Sonkoue,
Pamela Kenfack,
Kelly Djeuga,
Christine Schippa,
Elisabet Dunach,
Jackson Armel Seukep,
Stephen Tamekou Lacmata,
Theophile Fonkou,
Leon Azefack Tapondjou,
Hilaire Macaire Womeni
Issue:
Volume 7, Issue 1, March 2021
Pages:
14-21
Received:
31 January 2021
Accepted:
8 February 2021
Published:
27 February 2021
Abstract: Essential oils (EOs) have aroused attention among the naturally-occurring therapeutic compounds with anti-infective properties. This study examined the chemical composition and the antibacterial potential of EOs from three Lamiaceae species including Ocimum gratissimum (leaves), Plecthrantus epilithicus (leaves), and Satureja robusta (leaves and flowers). EOs were obtained by hydrodistillation using a Clevenger-type apparatus, followed by characterization by gas chromatography-mass spectrometry (GC-MS) and flame ionization detector (GC/FID). The antibacterial efficacy of EOs was screened using the microdilution method, against a panel of eight foodborne bacteria isolates namely Enterobacter cloacae, Yersinia enterocolotica, Klebsiella pneumoniae, Salmonella typhi, Escherichia coli, Citrobacter freundii, Shigella flexneri, and Enterococcus faecalis. GC-MS and GC/FID analysis led to the identification of 53 components from EOs of P. epilithicus, while around forty compounds have been characterized from the EOs of O. gratissimum (43), leaves of Satureja robusta (43), and flowers of S. robusta (44). The nature of identified compounds varied according to the species. The most prevalent volatile compounds identified in the EOs of P. epilithicus were piperitenone oxide (23.65%) and two isomers piperitone oxide 2/2 (16.15%) and piperitone oxide 1/2 (7.24%). The major constituents in the EOs of leaves of O. gratissimum were elemicin (33.474%) and eugenol (30.258%). Piperitone oxide, piperitone, and germacrene D were identified as major constituents in both the EOs of leaves of S. robusta (28.3%, 15.14%, and 9.42% respectively) and flowers (45.6%, 11.55%, and 3.94% respectively). The oils displayed selective antibacterial potential, with the recorded minimal inhibitory concentration (MIC) values ranging from 0.0156 to 1% (v/v). EOs of P. epilithicus as well as that of the leaves and flowers of S. robusta acted against all studied bacteria. Most of the MIC values were below 0.25% (v/v), indicating a strong inhibitory potential of studied EOs. The present study provides a strong baseline for consideration of the EOs from O. gratissimum, S. robusta, and P. epilithicus in the control of bacterial foodborne infections.
Abstract: Essential oils (EOs) have aroused attention among the naturally-occurring therapeutic compounds with anti-infective properties. This study examined the chemical composition and the antibacterial potential of EOs from three Lamiaceae species including Ocimum gratissimum (leaves), Plecthrantus epilithicus (leaves), and Satureja robusta (leaves and fl...
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Anti-arthritic Activity of the Aqueous Extract of the Aerial Parts of Mitracarpus scaber (Rubiaceae) Zucc in Rats Wistar
Georges Abizi,
Ouattara-Soro Fatou Scherazade,
Ernest Zougrou N’guessan,
John Kouadio Kouakou,
Emile Begbin Kouassi,
Sévérin Koffi,
Jean-Jacques Kablan Kassi,
Alassane Koné
Issue:
Volume 7, Issue 1, March 2021
Pages:
22-29
Received:
22 February 2021
Accepted:
8 March 2021
Published:
26 March 2021
Abstract: Arthritis is an autoimmune disease characterized by joint inflammation, the treatment of this disease requires the use of non-steroidal or steroidal anti-inflammatory drugs. Given the many side effects associated with conventional anti-inflammatory drugs, the use of herbal medicines would benefit patients. Mitracarpus scaber (Rubiaceae), is a herb whose leaves are used to treat skin conditions and relieve pain in the limbs. To elucidate the traditional use of the aerial parts of M. scaber, the present study was conducted on arthritic rats induced by Freund's complete adjuvant. Arthritis was induced in Wistar rats by injecting Freund's complete adjuvant solution into the right hind leg. Diclofenac and Celebrex were chosen as reference molecules. Administration of Diclofenac (5 mg/kg body weight) and Celebrex (10 mg/kg body weight) and the aqueous extract of the aerial parts of M. scaber at doses of 200; 400 and 800 mg/kg body weight began the day after injection and continued for 20 days. Leg swelling, biochemical and hematological parameters as well as fibrinogen levels were evaluated. Oral administration of the extract significantly suppressed inflammatory responses in the joints of arthritic rats. It did not cause liver damage in the rats during the twenty (20) days of treatment. These results indicate that the aqueous extract of the aerial parts of M. scaber prevents the pathological development of arthritis in rats. The active ingredients present in the extract could be potential agents for the treatment of inflammation and arthritis.
Abstract: Arthritis is an autoimmune disease characterized by joint inflammation, the treatment of this disease requires the use of non-steroidal or steroidal anti-inflammatory drugs. Given the many side effects associated with conventional anti-inflammatory drugs, the use of herbal medicines would benefit patients. Mitracarpus scaber (Rubiaceae), is a herb ...
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