| Peer-Reviewed

Cotton Bed Promotes Faster Growth and Higher Biomass Production of Mat-Forming Cyanobacterium Oscillatoria sp.

Received: 23 April 2022    Accepted: 9 May 2022    Published: 16 June 2022
Views:       Downloads:
Abstract

The high biomass is the fundamental requisite for harnessing the commercial potentials of cyanobacteria. The present study demonstrates the faster production of high biomass of Oscillatoria sp. in the open-tray culture system. Oscillatoria sp. requires a suitable surface for forming its mat; therefore, initially, we have screened cotton, rice husk, gravels, and mud as supporting beds for the quicker growth of the Oscillatoria sp. Based on the expansion rate, the development of the Oscillatoria mat was the fastest on the cotton bed. Based on the result, the Oscillatoria mat grown on the cotton bed was superior in thickness, biomass quantity, and ability to produce phycocyanin to the naturally growing Oscillatoria mat. The Oscillatoria mat on the cotton bed was approximately 57.0% thicker than the naturally growing mat of Oscillatoria sp. Similarly, the Oscillatorial mat on the cotton bed generates almost double biomass and 15.0% higher phycocyanin content than that of the naturally growing mat of Oscillatoria sp. Finally, we compared four different harvesting methods, e. g. wiping, squeezing, centrifugation and vortexing with centrifugation for their efficiency to harvest the Oscillatoria biomass generated on the cotton bed. These four methods harvested more than 85.0% of the biomass of Osciallatoria sp., with more than 96.0% harvesting efficiency wiping was recorded as the most efficient harvesting method followed by vortexing and centrifugation with approximately 94.0% harvesting efficiency. The present findings suggest using the cotton bed as a supporting surface for the quick generation of a high volume of biomass of Oscillatoria sp. in the open culture system.

Published in American Journal of Chemical and Biochemical Engineering (Volume 6, Issue 1)
DOI 10.11648/j.ajcbe.20220601.15
Page(s) 36-43
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Algal Culture, Biomass, Cyanobacterium, Green Energy, Oscillatoria

References
[1] Oliver RL, Ganf GG (2000) Freshwater Blooms. In: Whitton BA, Potts M (eds) The Ecology of Cyanobacteria. Springer, Dordrecht. pp 149-194.
[2] Schrader KK, Nanayakkara NPD, Tucker CS, Rimando AM, Ganzera M, Schaneberg BT (2003) Novel derivatives of 9,10-anthraquinone are selective algicides against the musty-odor cyanobacterium Oscillatoria perornata. App Environ Microbiol 69: 5319-5327.
[3] Nunnery JK, Mevers E, Gerwick WH (2010) Biologically active secondary metabolites from marine cyanobacteria, Cur Opin Biotechnol 21: 787-793.
[4] Harada KI, Suomalainen M, Uchida H, Masui H, Ohmura K, Kiviranta J, Niku-Paavola ML, Ikemoto T (2000) Insecticidal compounds against mosquito larvae from Oscillatoria agardhii strain 27. Environ Toxicol 15: 114-119.
[5] Bhuyar P, Rahim MHA, Maniam GP, Ramaraj R, Govindan N 92020) Exploration of bioactive compounds and antibacterial activity of marine blue-green microalgae (Oscillatoria sp.) isolated from coastal region of west Malaysia. SN Appl Sci 2: 1-10.
[6] Nainangu P, Antonyraj APM, Subramanian K, Kaliyaperumal S, Gopal S et al. (2020) In vitro screening of antimicrobial, antioxidant, cytotoxic activities, and characterization of bioactive substances from freshwater cyanobacteria Oscillatoria sp. SSCM01 and Phormidium sp. SSCM02. Biocatal Agric Biotechnol 29: 101772.
[7] Rajavel R, Mallika P, Rajesh V, Kumar KP, Krishna Moorthy S, Sivakumar T (2012) Antinociceptive and anti-inflammatory effects of the methanolic extract of Oscillatoria annae. Res J Chem Sci 2: 53-61.
[8] Singh V, Tripathi BN (2012) Cyanobacterial toxins: types, mechanisms of action, ecological roles and potential application as commercial compounds. In: Pandey VD, Singh SK (eds) Microbial Toxins and Toxigenic Microbes. Studium Press LLC, Houston, USA pp 395-410.
[9] Kawale HD, Kishore N (2019) Production of hydrocarbons from a green algae (Oscillatoria) with exploration of its fuel characteristics over different reaction atmospheres, Energy 178: 344-355.
[10] Bhatia SK, Mehariya S, Bhatia RK, Kumar M, Pugazhendhi A, Awasthi M. K, Atabani AE, Kumar G, Kim W, Seo, SO, Yang YH (2021) Wastewater based microalgal biorefinery for bioenergy production: Progress and challenges. Sci Total Environ 751: 141599.
[11] Kallarakkal KP, Muthukumar K, Alagarsamy A, Pugazhendhi A, Mohamed SA (2021) Enhancement of biobutanol production using mixotrophic culture of Oscillatoria sp. in cheese whey water. Fuel 284: 119008.
[12] Riskuwa-Shehu ML, Ismail HY, Maimuna Sulaiman M (2019) Biosorption of heavy metals by Oscillatoria Species. Microbiol. Res J Int 27: 1-8.
[13] Bon IC, Salvatierra LM, Lario LD, Morató J, Leonardo M, Pérez LM (2021) Prospects in cadmium-contaminated water management using free-living cyanobacteria (Oscillatoria sp.). Water 13: 542.
[14] Kumar D, Prakash B, Pandey LK, Gaur JP (2010) Sorption of paraquat and 2,4-D by an Oscillatoria Sp.-dominated cyanobacterial mat. Appl Biochem Biotechnol 160: 2475-2485.
[15] Pathak J, Maurya PK, Singh SP, Häder DP, Sinha RP (2018) Cyanobacterial farming for environment friendly sustainable agriculture practices: innovations and perspectives. Front Environ Sc 6: 1-13.
[16] Desikachary TV (1959) Cyanophyta. Indian Council of Agriculture Research, New Delhi.
[17] Hughes EO, Gorham PR, Zehnder A (1958) Toxicity of a unialgal culture of Microcystis aeruginosa. Can J Microbiol 4: 225-236.
[18] Tripathi BN, Mehta SK, Gaur JP (2003) Differential sensitivity of the cyanobacterium Anabaena doliolum to Cu and Zn in batch and semicontinuous cultures. Ecotoxicol. Environ Saf 56: 311-318.
[19] Mackinney G (1941) Absorption of light by chlorophyll solutions. J Biol Chem 140: 315-322.
[20] Zavřel T, Chmelík D, Sinetova MA, Červený J (2018) Spectrophotometric determination of phycobiliprotein content in cyanobacterium Synechocystis. J Vis Exp 139: 58076.
[21] Zhang Q, Yu Z, Jin S, Zhu L, Liu C, Zheng H, Zhou T, Liu Y, Ruan R (2019) Lignocellulosic residue as bio-carrier for algal biofilm growth: Effects of carrier physicochemical proprieties and toxicity on algal biomass production and composition. Bioresource Technology 293: 122091.
[22] Delgado A, Gómez JA (2016) The soil, physical, chemical and biological properties. In: Villalobos, F, Fereres E (eds) Principles of Agronomy for Sustainable Agriculture. Springer, Cham pp 15-26.
[23] Sterner RW (1989) Resource competition during seasonal succession toward dominance by cyanobacteria. Ecology 70: 229-245.
[24] Hassan MF, Lee HP, Lim S (2012) Effects of shear and surface roughness on reducing the attachment of Oscillatoria sp. filaments on substrates. Water Environ Res 84: 744-752.
[25] Zuorro A, Leal-Jerez AG, Morales-Rivas LK, Mogollón-Londoño SO, Sanchez-Galvis EM, García-Martínez JB, Andrés F, Barajas-Solano AF (2021) Enhancement of phycobiliprotein accumulation in thermotolerant Oscillatoria sp. through media pptimization. ACS Omega 6: 10527-10536.
Cite This Article
  • APA Style

    Vijetna Singh, Bhumi Nath Tripathi. (2022). Cotton Bed Promotes Faster Growth and Higher Biomass Production of Mat-Forming Cyanobacterium Oscillatoria sp.. American Journal of Chemical and Biochemical Engineering, 6(1), 36-43. https://doi.org/10.11648/j.ajcbe.20220601.15

    Copy | Download

    ACS Style

    Vijetna Singh; Bhumi Nath Tripathi. Cotton Bed Promotes Faster Growth and Higher Biomass Production of Mat-Forming Cyanobacterium Oscillatoria sp.. Am. J. Chem. Biochem. Eng. 2022, 6(1), 36-43. doi: 10.11648/j.ajcbe.20220601.15

    Copy | Download

    AMA Style

    Vijetna Singh, Bhumi Nath Tripathi. Cotton Bed Promotes Faster Growth and Higher Biomass Production of Mat-Forming Cyanobacterium Oscillatoria sp.. Am J Chem Biochem Eng. 2022;6(1):36-43. doi: 10.11648/j.ajcbe.20220601.15

    Copy | Download

  • @article{10.11648/j.ajcbe.20220601.15,
      author = {Vijetna Singh and Bhumi Nath Tripathi},
      title = {Cotton Bed Promotes Faster Growth and Higher Biomass Production of Mat-Forming Cyanobacterium Oscillatoria sp.},
      journal = {American Journal of Chemical and Biochemical Engineering},
      volume = {6},
      number = {1},
      pages = {36-43},
      doi = {10.11648/j.ajcbe.20220601.15},
      url = {https://doi.org/10.11648/j.ajcbe.20220601.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcbe.20220601.15},
      abstract = {The high biomass is the fundamental requisite for harnessing the commercial potentials of cyanobacteria. The present study demonstrates the faster production of high biomass of Oscillatoria sp. in the open-tray culture system. Oscillatoria sp. requires a suitable surface for forming its mat; therefore, initially, we have screened cotton, rice husk, gravels, and mud as supporting beds for the quicker growth of the Oscillatoria sp. Based on the expansion rate, the development of the Oscillatoria mat was the fastest on the cotton bed. Based on the result, the Oscillatoria mat grown on the cotton bed was superior in thickness, biomass quantity, and ability to produce phycocyanin to the naturally growing Oscillatoria mat. The Oscillatoria mat on the cotton bed was approximately 57.0% thicker than the naturally growing mat of Oscillatoria sp. Similarly, the Oscillatorial mat on the cotton bed generates almost double biomass and 15.0% higher phycocyanin content than that of the naturally growing mat of Oscillatoria sp. Finally, we compared four different harvesting methods, e. g. wiping, squeezing, centrifugation and vortexing with centrifugation for their efficiency to harvest the Oscillatoria biomass generated on the cotton bed. These four methods harvested more than 85.0% of the biomass of Osciallatoria sp., with more than 96.0% harvesting efficiency wiping was recorded as the most efficient harvesting method followed by vortexing and centrifugation with approximately 94.0% harvesting efficiency. The present findings suggest using the cotton bed as a supporting surface for the quick generation of a high volume of biomass of Oscillatoria sp. in the open culture system.},
     year = {2022}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Cotton Bed Promotes Faster Growth and Higher Biomass Production of Mat-Forming Cyanobacterium Oscillatoria sp.
    AU  - Vijetna Singh
    AU  - Bhumi Nath Tripathi
    Y1  - 2022/06/16
    PY  - 2022
    N1  - https://doi.org/10.11648/j.ajcbe.20220601.15
    DO  - 10.11648/j.ajcbe.20220601.15
    T2  - American Journal of Chemical and Biochemical Engineering
    JF  - American Journal of Chemical and Biochemical Engineering
    JO  - American Journal of Chemical and Biochemical Engineering
    SP  - 36
    EP  - 43
    PB  - Science Publishing Group
    SN  - 2639-9989
    UR  - https://doi.org/10.11648/j.ajcbe.20220601.15
    AB  - The high biomass is the fundamental requisite for harnessing the commercial potentials of cyanobacteria. The present study demonstrates the faster production of high biomass of Oscillatoria sp. in the open-tray culture system. Oscillatoria sp. requires a suitable surface for forming its mat; therefore, initially, we have screened cotton, rice husk, gravels, and mud as supporting beds for the quicker growth of the Oscillatoria sp. Based on the expansion rate, the development of the Oscillatoria mat was the fastest on the cotton bed. Based on the result, the Oscillatoria mat grown on the cotton bed was superior in thickness, biomass quantity, and ability to produce phycocyanin to the naturally growing Oscillatoria mat. The Oscillatoria mat on the cotton bed was approximately 57.0% thicker than the naturally growing mat of Oscillatoria sp. Similarly, the Oscillatorial mat on the cotton bed generates almost double biomass and 15.0% higher phycocyanin content than that of the naturally growing mat of Oscillatoria sp. Finally, we compared four different harvesting methods, e. g. wiping, squeezing, centrifugation and vortexing with centrifugation for their efficiency to harvest the Oscillatoria biomass generated on the cotton bed. These four methods harvested more than 85.0% of the biomass of Osciallatoria sp., with more than 96.0% harvesting efficiency wiping was recorded as the most efficient harvesting method followed by vortexing and centrifugation with approximately 94.0% harvesting efficiency. The present findings suggest using the cotton bed as a supporting surface for the quick generation of a high volume of biomass of Oscillatoria sp. in the open culture system.
    VL  - 6
    IS  - 1
    ER  - 

    Copy | Download

Author Information
  • Department of Biotechnology, Indira Gandhi National Tribal University, Amarkantak, India

  • Department of Biotechnology, Indira Gandhi National Tribal University, Amarkantak, India

  • Sections