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Isolation, Identification and Antibiotic Susceptibility Pattern of Bacteria Isolated from Wounds of Patients Attending at Arsho Advanced Medical Laboratory

Received: 9 March 2018     Accepted: 2 April 2018     Published: 11 May 2018
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Abstract

Wound infections cause clinical and societal consequences on the patients, but its bacteriological characteristicvaries with different factors. Therefore, effective treatment and management of wound infections in hospital and communitysetting will require detailed epidemiological knowledge of the infecting bacterial pathogens and their antibiogramunusual to theenvironment. Based on this information, we examined the frequency and antibiogram of bacterial pathogens isolated from woundinfection cases seen at ArshoAdvancedmedical laboratory over the study period. A total of 259 wound swabs/ and pus of different types of woundinfections from different anatomical sites were analyzed by standard bacteriological methods. Of the 259 clinical specimens analyzed, 177 (68.4%) yielded at least one bacterial pathogens, 6(2.3%) were polymicrobial, and 82(31.6%) yielded no bacterial growth. Overall, 20 different bacterial pathogens were identified 15 (75%) gram-negative bacteria isolates and 5(25%) gram-positive bacterial isolated. Staphylococcus aureus accounted formajority of the bacterial pathogens isolated, 86 (48.6% followed by E.coli20 (11.3%, and Citrobacterspp. 17(9.6%). The bacterial pathogens demonstrated high resistance to amoxicillin (79.7 %%), ampicillin (78.3%), and tetracycline (73.1%), in contrastto high sensitivity pattern observed with Meropinem (94.5%), Levofloxacin (87%), Amikacin (82.4%), and Ceftazidime (72.7%). Amikacin, meropenem and levofloxacin were the most effective drugs against the tested gram- positive and -negative bacteria and should be considered in empirical antibiotic selection.

Published in Science Journal of Clinical Medicine (Volume 7, Issue 2)
DOI 10.11648/j.sjcm.20180702.12
Page(s) 20-24
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Wound Infections, Bacterial Pathogens, Polymicrobial, Arsho

References
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[2] Egbe CA, Omoregie R, Igbarumah IO, Onemu S. Microbiology of wound infections and its associated risk factors among patients of a Tertiary hospital in Benin City, Nigeria. JRHS. 2011; 11(2):109-113.
[3] Bowler PG, Duerden BI and Armstrong DG. Wound microbiology and associated approaches to wound management. Clinical Microbiology Review. 2001; 14:244-269.
[4] Sibor JO, Oseni A, Eyaufe A, Osagie R. Turay A. Incidence of aerobic bacteria and Candida albicas in post operative wound infections. Afr J Microbiol Res. 2008; 2:288-291.
[5] Aynalem Mohammed, Mengistu Endris Seid, Teklay Gebrecherkos, Moges Tiruneh, and Feleke Moges, “Bacterial Isolates and Their Antimicrobial Susceptibility Patterns of Wound Infections among Inpatients and Outpatients Attending the University of Gondar Referral Hospital, Northwest Ethiopia,” International Journal of Microbiology, 2017, 2017; 10.
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[9] Bularafa Mohammed Yasidiet al.: Retrospective Analysis of Bacterial Pathogens Isolated from Wound Infections at a Tertiary Hospital in Nguru, Yobe State Nigeria.
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[13] Muluye D, Wondimeneh Y, Ferede G, Nega T, Adane K, Biadgo B, Tesfa H, Moges. Bacterial isolates and their antibiotic susceptibility patterns among patients with pus and/or wound discharge at Gondar University hospital. BMC Research Notes. 2014; 7:619.
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[15] Adane Bitew, Mesele Admassie, Tigist Getachew. Spectrum and Drug Susceptibility Profile of Bacteria Recovered from Patients with wound Infection Referred to Arsho Advanced Medical Laboratory. Clinical Medicine Research. 2018;7 (1): 8-17.
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[17] Syed AsadAli, S. M. Tahir, Abdul SattarMemon, Noshad A. Shaikh. Pattern of pathogens and their sensitivity isolated from superficial surgical site infections in a tertiary care hospital. JAyub Med Coll Abbottabad. 2009; 21(2).
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  • APA Style

    Messele Admassie, Estifanos Tsige, Meseret Chanie. (2018). Isolation, Identification and Antibiotic Susceptibility Pattern of Bacteria Isolated from Wounds of Patients Attending at Arsho Advanced Medical Laboratory. Science Journal of Clinical Medicine, 7(2), 20-24. https://doi.org/10.11648/j.sjcm.20180702.12

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    ACS Style

    Messele Admassie; Estifanos Tsige; Meseret Chanie. Isolation, Identification and Antibiotic Susceptibility Pattern of Bacteria Isolated from Wounds of Patients Attending at Arsho Advanced Medical Laboratory. Sci. J. Clin. Med. 2018, 7(2), 20-24. doi: 10.11648/j.sjcm.20180702.12

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    AMA Style

    Messele Admassie, Estifanos Tsige, Meseret Chanie. Isolation, Identification and Antibiotic Susceptibility Pattern of Bacteria Isolated from Wounds of Patients Attending at Arsho Advanced Medical Laboratory. Sci J Clin Med. 2018;7(2):20-24. doi: 10.11648/j.sjcm.20180702.12

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  • @article{10.11648/j.sjcm.20180702.12,
      author = {Messele Admassie and Estifanos Tsige and Meseret Chanie},
      title = {Isolation, Identification and Antibiotic Susceptibility Pattern of Bacteria Isolated from Wounds of Patients Attending at Arsho Advanced Medical Laboratory},
      journal = {Science Journal of Clinical Medicine},
      volume = {7},
      number = {2},
      pages = {20-24},
      doi = {10.11648/j.sjcm.20180702.12},
      url = {https://doi.org/10.11648/j.sjcm.20180702.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjcm.20180702.12},
      abstract = {Wound infections cause clinical and societal consequences on the patients, but its bacteriological characteristicvaries with different factors. Therefore, effective treatment and management of wound infections in hospital and communitysetting will require detailed epidemiological knowledge of the infecting bacterial pathogens and their antibiogramunusual to theenvironment. Based on this information, we examined the frequency and antibiogram of bacterial pathogens isolated from woundinfection cases seen at ArshoAdvancedmedical laboratory over the study period. A total of 259 wound swabs/ and pus of different types of woundinfections from different anatomical sites were analyzed by standard bacteriological methods. Of the 259 clinical specimens analyzed, 177 (68.4%) yielded at least one bacterial pathogens, 6(2.3%) were polymicrobial, and 82(31.6%) yielded no bacterial growth. Overall, 20 different bacterial pathogens were identified 15 (75%) gram-negative bacteria isolates and 5(25%) gram-positive bacterial isolated. Staphylococcus aureus accounted formajority of the bacterial pathogens isolated, 86 (48.6% followed by E.coli20 (11.3%, and Citrobacterspp. 17(9.6%). The bacterial pathogens demonstrated high resistance to amoxicillin (79.7 %%), ampicillin (78.3%), and tetracycline (73.1%), in contrastto high sensitivity pattern observed with Meropinem (94.5%), Levofloxacin (87%), Amikacin (82.4%), and Ceftazidime (72.7%). Amikacin, meropenem and levofloxacin were the most effective drugs against the tested gram- positive and -negative bacteria and should be considered in empirical antibiotic selection.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Isolation, Identification and Antibiotic Susceptibility Pattern of Bacteria Isolated from Wounds of Patients Attending at Arsho Advanced Medical Laboratory
    AU  - Messele Admassie
    AU  - Estifanos Tsige
    AU  - Meseret Chanie
    Y1  - 2018/05/11
    PY  - 2018
    N1  - https://doi.org/10.11648/j.sjcm.20180702.12
    DO  - 10.11648/j.sjcm.20180702.12
    T2  - Science Journal of Clinical Medicine
    JF  - Science Journal of Clinical Medicine
    JO  - Science Journal of Clinical Medicine
    SP  - 20
    EP  - 24
    PB  - Science Publishing Group
    SN  - 2327-2732
    UR  - https://doi.org/10.11648/j.sjcm.20180702.12
    AB  - Wound infections cause clinical and societal consequences on the patients, but its bacteriological characteristicvaries with different factors. Therefore, effective treatment and management of wound infections in hospital and communitysetting will require detailed epidemiological knowledge of the infecting bacterial pathogens and their antibiogramunusual to theenvironment. Based on this information, we examined the frequency and antibiogram of bacterial pathogens isolated from woundinfection cases seen at ArshoAdvancedmedical laboratory over the study period. A total of 259 wound swabs/ and pus of different types of woundinfections from different anatomical sites were analyzed by standard bacteriological methods. Of the 259 clinical specimens analyzed, 177 (68.4%) yielded at least one bacterial pathogens, 6(2.3%) were polymicrobial, and 82(31.6%) yielded no bacterial growth. Overall, 20 different bacterial pathogens were identified 15 (75%) gram-negative bacteria isolates and 5(25%) gram-positive bacterial isolated. Staphylococcus aureus accounted formajority of the bacterial pathogens isolated, 86 (48.6% followed by E.coli20 (11.3%, and Citrobacterspp. 17(9.6%). The bacterial pathogens demonstrated high resistance to amoxicillin (79.7 %%), ampicillin (78.3%), and tetracycline (73.1%), in contrastto high sensitivity pattern observed with Meropinem (94.5%), Levofloxacin (87%), Amikacin (82.4%), and Ceftazidime (72.7%). Amikacin, meropenem and levofloxacin were the most effective drugs against the tested gram- positive and -negative bacteria and should be considered in empirical antibiotic selection.
    VL  - 7
    IS  - 2
    ER  - 

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Author Information
  • Department of Microbiology, Arsho Advanced Medical Laboratory, Addis Ababa, Ethiopia

  • Department of Microbiology, Arsho Advanced Medical Laboratory, Addis Ababa, Ethiopia

  • Department of Microbiology, Arsho Advanced Medical Laboratory, Addis Ababa, Ethiopia

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