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Enzymatic Biosensor Associated with Molecularly Imprinted Polymers for Sensitive and Selective Detection of Organophosphorus Insecticides in Olive Oil

Received: 13 September 2014     Accepted: 17 September 2014     Published: 15 October 2014
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Abstract

5 different bio-tools have been developed for sensitive detection of three organophosphorus insecticides mainly used for the treatment of olive trees: Malaoxon, Omethoate and Methidathion-oxon. The systems are based on the immobilization of electric eel acetylcholinesterase on screen-printed electrode (SPE) and microtiter plates by 3 different methods of immobilization. These developed systems were optimized with laboratory samples and then tested on natural samples of olive oil after a simple liquid-liquid extraction. Among these five systems designed, the amperometric biosensor based on acetylcholinesterase immobilized by bioencapsulation in a sol-gel matrix presented the best performance in terms of operational stability, storage stability, reproducibility, and it proved to be best suited for the determination of insecticides in real samples of olive oil. This biosensor has been associated with a highly selective extraction method based on the use of Molecularly Imprinted Polymers (MIPs) to improve selectivity. The results showed that the association MIPs-biosensor can selectively detect and quantify the three organophosphorus insecticides in such a complex matrix as olive oil, with limits of detection much lower than the maximum residue limit tolerated by international regulations.

Published in Science Innovation (Volume 2, Issue 6-1)

This article belongs to the Special Issue Biosensors: Innovative Technique for Food Safety and Environmental Monitoring

DOI 10.11648/j.si.s.2014020601.11
Page(s) 1-6
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Organophosphorus Insecticides, Electric Eel Acetylcholinesterase, Molecularly Imprinted Polymer (MIP), Biosensors, Olive Oil

References
[1] Gimeno, E., Fito, M., Lamuela-Raventos, R.M., Castellote, A.I., Covas, M., Farre, M. Europ. Effect of ingestion of virgin olive oil on human low-density lipoprotein composition. J. Clin. Nut. 56, 2002, 114-120.
[2] Lentza-Rizos, Ch. Insecticides authorized for use on olive trees and the relationship between their registration and residues in olive oil. Grasas Aceites. 47, 1996, 392-396.
[3] Lentza-Rizos, C., Avramides, E.J. Pesticide residues in olive oil. Arch. Environ. Contam. Toxicol. 141, 1995, 111-134.
[4] Rastrelli, L., Totaro, K., De Simone, F. Determination of organophosphorus pesticide residues in Cilento (Campania, Italy) virgin olive oil by capillary gas chromatography. Food Chemistry. 79, 2002, 303–305.
[5] Cabras, P., Angioni, A., Garau, V.L., Melis, M., Pirisi, F.M., Karim, M., Minelli, E.V. Persistence of insecticide residues in olives and olive oil. J. Agric. Food. Chem. 45, 1997, 2244- 2247
[6] Guardia-Rubio, M., Marchal-Lopez, R.M., Ayora-Canada, M.J., Ruiz-Medina, A. Determination of pesticides in olives by gas chromatography using different detection systems. J. Chromatogr. A.1145, 2007, 195–203.
[7] Ferrer, C., Gomez, M. J., Garcia-Reyes, J. F., Ferrer, I., Thurman, E. M., Fernandez-Alba, A R. Determination of pesticide residues in olives and olive oil by matrix solid-phase dispersion followed by gas chromatography/mass spectrometry and liquid chromatography/tandem mass spectrometry. J. Chromatogr. A.1069, 2005, 183-194.
[8] Andreescu, S., Barthelmebs, L., Marty, J-L. Anal. Immobilization of acetylcholinesterase on screen-printed electrodes: comparative study between three immobilization methods and applications to the detection of organophosphorus insecticides. Chim. Acta., 464, 2002, 171–180.
[9] Ben Oujji, N., Bakas, I., Istamboulié, G., Ait-Ichou, I., Ait-Addi, E., Rouillon, R., Noguer, T. A Simple Colorimetric Enzymatic-Assay, based on immobilization of acetylcholinesterase by adsorption, for sensitive detection of organophosphorus insecticides in olive oil. Food Control. 46, 2014, 75-80.
[10] Ben Oujji, N., Bakas, I., Istamboulié, G., Ait-Ichou, I., Ait-Addi, E., Rouillon, R., Noguer, T. Acetylcholinesterase immobilized on magnetic beads for pesticides detection: application to olive oil analysis. Sensors, 12, 2012, 7893-7904.
[11] Ben Oujji, N., Bakas, I., Istamboulié, G., Ait-Ichou, I., Ait-Addi, E., Rouillon, R., Noguer, T. Sol-gel immobilization of acetylcholinesterase for the determination of organophosphate pesticides in olive oil with biosensors. Food Control, 30, 2013, 657-661.
[12] Istamboulie, G., Fournier, D., Marty, J-L., Noguer, T. Phosphotriesterase: A complementary tool for the selective detection of two organophosphate insecticides: Chlorpyrifos and chlorfenvinfos. Talanta. 77, 2009, 1627–1631.
[13] Rhouati, A., Istamboulie, G., Cortina-Puig, M., Marty, J-L., Noguer, T. Selective spectrophotometric detection of insecticides using cholinesterases, phosphotriesterase and chemometric analysis. Enzyme. Microb. Tech., 46, 2010, 212–216.
[14] Istamboulie, G., Cortina-Puig, M., Marty, J-L., Noguer, T. The use of Artificial Neural Networks for the selective detection of two organophosphate insecticides: Chlorpyrifos and chlorfenvinfos. Talanta, 2009, 79, 507–511.
[15] Alonso, G., Istamboulie, G., Ramírez-García, A., Noguer, T., Marty J-L, Munoza, R. Comput. Artificial neural network implementation in single low-cost chip for the detection of insecticides by modelling of screen-printed enzymatic sensors response. Computers and Electronics in Agriculture Electron. Agric. 74, 2010, 223-229.
[16] Lok, C. M. and Son, R. Application of molecularly imprinted polymers in food sample analysis-a perspective. Food Res. Int. 16, 2009, 127-140.
[17] Piletsky, S.A., Turner, N.W. and Laitenberger, P. Molecularly imprinted polymers in clinical diagnostics, future potential and existing problems. Med. Eng. Phys. 28, 2006, 971-977.
[18] Bakas, I., Ben Oujji, N., Moczko, E., Istamboulie, G., Piletsky, S., Piletska, E., Ait- Ichou, I., Ait-Addi, E., Noguer, T., Rouillon, R. Molecular imprinting solid phase extraction for selective detection of methidathion in olive oil. Anal. Chim. Acta, 734, 2012, 99-105.
[19] Bakas, I., Ben Oujji, N., Moczko, E., Istamboulie, G., Piletsky, S., Piletska, E., Ait- Ichou, I., Ait-Addi, E., Noguer, T., Rouillon, R. J. Computational and experimental investigation of molecular imprinted polymers for selective extraction of dimethoate and its metabolite omethoate from olive oil. Chromatogr. A. 1274, 2013, 13–18.
[20] Bakas, I., Ben Oujji, N., Istamboulie, G., Piletsky, S., Piletska, E., Ait-Addi, E., Ait-Ichou, I., Noguer, T., Rouillon, R. Molecularly imprinted polymer cartridges coupled to high performance liquid chromatography (HPLC-UV) for simple and rapid analysis of fenthion in olive oil. Talanta. 125, 2014, 313–318
[21] Ellman, G.L., Courtney, K.D., Andres, V., Featherstone, R.M. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 7 (1961) 88–92.
[22] Hayat, A., Barthelmebs, L., Marty, J.-L. A simple colorimetric enzymatic-assay for okadaic acid detection based on the immobilization of protein phosphatase 2A in sol-gel. Appl. Biochem. Biotech., 2012, 166, 47-56.
Cite This Article
  • APA Style

    Ben Oujji Najwa, Bakas Idriss, Istamboulie Georges, Ait-Ichou Ihya, Ait-Addi Elhabib, et al. (2014). Enzymatic Biosensor Associated with Molecularly Imprinted Polymers for Sensitive and Selective Detection of Organophosphorus Insecticides in Olive Oil. Science Innovation, 2(6-1), 1-6. https://doi.org/10.11648/j.si.s.2014020601.11

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    ACS Style

    Ben Oujji Najwa; Bakas Idriss; Istamboulie Georges; Ait-Ichou Ihya; Ait-Addi Elhabib, et al. Enzymatic Biosensor Associated with Molecularly Imprinted Polymers for Sensitive and Selective Detection of Organophosphorus Insecticides in Olive Oil. Sci. Innov. 2014, 2(6-1), 1-6. doi: 10.11648/j.si.s.2014020601.11

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    AMA Style

    Ben Oujji Najwa, Bakas Idriss, Istamboulie Georges, Ait-Ichou Ihya, Ait-Addi Elhabib, et al. Enzymatic Biosensor Associated with Molecularly Imprinted Polymers for Sensitive and Selective Detection of Organophosphorus Insecticides in Olive Oil. Sci Innov. 2014;2(6-1):1-6. doi: 10.11648/j.si.s.2014020601.11

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  • @article{10.11648/j.si.s.2014020601.11,
      author = {Ben Oujji Najwa and Bakas Idriss and Istamboulie Georges and Ait-Ichou Ihya and Ait-Addi Elhabib and Rouillon Régis and Noguer Thierry},
      title = {Enzymatic Biosensor Associated with Molecularly Imprinted Polymers for Sensitive and Selective Detection of Organophosphorus Insecticides in Olive Oil},
      journal = {Science Innovation},
      volume = {2},
      number = {6-1},
      pages = {1-6},
      doi = {10.11648/j.si.s.2014020601.11},
      url = {https://doi.org/10.11648/j.si.s.2014020601.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.si.s.2014020601.11},
      abstract = {5 different bio-tools have been developed for sensitive detection of three organophosphorus insecticides mainly used for the treatment of olive trees: Malaoxon, Omethoate and Methidathion-oxon. The systems are based on the immobilization of electric eel acetylcholinesterase on screen-printed electrode (SPE) and microtiter plates by 3 different methods of immobilization. These developed systems were optimized with laboratory samples and then tested on natural samples of olive oil after a simple liquid-liquid extraction. Among these five systems designed, the amperometric biosensor based on acetylcholinesterase immobilized by bioencapsulation in a sol-gel matrix presented the best performance in terms of operational stability, storage stability, reproducibility, and it proved to be best suited for the determination of insecticides in real samples of olive oil. This biosensor has been associated with a highly selective extraction method based on the use of Molecularly Imprinted Polymers (MIPs) to improve selectivity. The results showed that the association MIPs-biosensor can selectively detect and quantify the three organophosphorus insecticides in such a complex matrix as olive oil, with limits of detection much lower than the maximum residue limit tolerated by international regulations.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - Enzymatic Biosensor Associated with Molecularly Imprinted Polymers for Sensitive and Selective Detection of Organophosphorus Insecticides in Olive Oil
    AU  - Ben Oujji Najwa
    AU  - Bakas Idriss
    AU  - Istamboulie Georges
    AU  - Ait-Ichou Ihya
    AU  - Ait-Addi Elhabib
    AU  - Rouillon Régis
    AU  - Noguer Thierry
    Y1  - 2014/10/15
    PY  - 2014
    N1  - https://doi.org/10.11648/j.si.s.2014020601.11
    DO  - 10.11648/j.si.s.2014020601.11
    T2  - Science Innovation
    JF  - Science Innovation
    JO  - Science Innovation
    SP  - 1
    EP  - 6
    PB  - Science Publishing Group
    SN  - 2328-787X
    UR  - https://doi.org/10.11648/j.si.s.2014020601.11
    AB  - 5 different bio-tools have been developed for sensitive detection of three organophosphorus insecticides mainly used for the treatment of olive trees: Malaoxon, Omethoate and Methidathion-oxon. The systems are based on the immobilization of electric eel acetylcholinesterase on screen-printed electrode (SPE) and microtiter plates by 3 different methods of immobilization. These developed systems were optimized with laboratory samples and then tested on natural samples of olive oil after a simple liquid-liquid extraction. Among these five systems designed, the amperometric biosensor based on acetylcholinesterase immobilized by bioencapsulation in a sol-gel matrix presented the best performance in terms of operational stability, storage stability, reproducibility, and it proved to be best suited for the determination of insecticides in real samples of olive oil. This biosensor has been associated with a highly selective extraction method based on the use of Molecularly Imprinted Polymers (MIPs) to improve selectivity. The results showed that the association MIPs-biosensor can selectively detect and quantify the three organophosphorus insecticides in such a complex matrix as olive oil, with limits of detection much lower than the maximum residue limit tolerated by international regulations.
    VL  - 2
    IS  - 6-1
    ER  - 

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Author Information
  • University of Perpignan, IMAGES EA4218, Building S 52 Av Paul Alduy, 66860 Perpignan Cedex, France

  • University of Perpignan, IMAGES EA4218, Building S 52 Av Paul Alduy, 66860 Perpignan Cedex, France

  • University of Perpignan, IMAGES EA4218, Building S 52 Av Paul Alduy, 66860 Perpignan Cedex, France

  • University Ibn Zohr, AQUAMAR, Photocatalysis and Environment Team, Chemistry Department/Faculty of Science, BP 8106 Citè Dakhla, Agadir, Morocco

  • University Ibn Zohr, AQUAMAR, Photocatalysis and Environment Team, Chemistry Department/Faculty of Science, BP 8106 Citè Dakhla, Agadir, Morocco

  • University of Perpignan, IMAGES EA4218, Building S 52 Av Paul Alduy, 66860 Perpignan Cedex, France

  • University of Perpignan, IMAGES EA4218, Building S 52 Av Paul Alduy, 66860 Perpignan Cedex, France

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