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Mechanism of Interaction Between Cefepime and Papain by Spectroscopic Method

Received: 27 January 2018     Accepted: 16 February 2018     Published: 19 March 2018
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Abstract

The reaction mechanism of cefepime (CEF) with papain (PAPA) was investigated by both fluorescence quenching and synchronous fluorescence spectroscopy in different temperature (293, 308 and 318 K). The results demonstrated that the interaction between CEF and PAPA was taking place via static quenching. Thermodynamic parameters revealed that electrostatic interaction played major roles in the interaction. The order of magnitudes of binding constant is 104, and the number of binding site in the system was closed to 1. The binding rates of CEF to PAPA (λex = 280 nm) were 81.46%~95.95% (293K), 80.50%~95.49% (308K) and 79.77%~95.12% (318K). There was a linear relationship between ΔF and the concentrations of CEF in the range of 6.0×10-6~7.0×10-5 mol/L. And the correlation coefficient of the linear curve was 0.9973.

Published in Journal of Photonic Materials and Technology (Volume 4, Issue 1)
DOI 10.11648/j.jmpt.20180401.16
Page(s) 33-38
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Cefepime, Papain, Reaction Mechanism, Spectroscopy, Determination

References
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  • APA Style

    Li Chen, Baosheng Liu, Chundan Wang, Lihua Ma, Hongcai Zhang, et al. (2018). Mechanism of Interaction Between Cefepime and Papain by Spectroscopic Method. Journal of Photonic Materials and Technology, 4(1), 33-38. https://doi.org/10.11648/j.jmpt.20180401.16

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    ACS Style

    Li Chen; Baosheng Liu; Chundan Wang; Lihua Ma; Hongcai Zhang, et al. Mechanism of Interaction Between Cefepime and Papain by Spectroscopic Method. J. Photonic Mater. Technol. 2018, 4(1), 33-38. doi: 10.11648/j.jmpt.20180401.16

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    AMA Style

    Li Chen, Baosheng Liu, Chundan Wang, Lihua Ma, Hongcai Zhang, et al. Mechanism of Interaction Between Cefepime and Papain by Spectroscopic Method. J Photonic Mater Technol. 2018;4(1):33-38. doi: 10.11648/j.jmpt.20180401.16

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  • @article{10.11648/j.jmpt.20180401.16,
      author = {Li Chen and Baosheng Liu and Chundan Wang and Lihua Ma and Hongcai Zhang and Xu Guo},
      title = {Mechanism of Interaction Between Cefepime and Papain by Spectroscopic Method},
      journal = {Journal of Photonic Materials and Technology},
      volume = {4},
      number = {1},
      pages = {33-38},
      doi = {10.11648/j.jmpt.20180401.16},
      url = {https://doi.org/10.11648/j.jmpt.20180401.16},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jmpt.20180401.16},
      abstract = {The reaction mechanism of cefepime (CEF) with papain (PAPA) was investigated by both fluorescence quenching and synchronous fluorescence spectroscopy in different temperature (293, 308 and 318 K). The results demonstrated that the interaction between CEF and PAPA was taking place via static quenching. Thermodynamic parameters revealed that electrostatic interaction played major roles in the interaction. The order of magnitudes of binding constant is 104, and the number of binding site in the system was closed to 1. The binding rates of CEF to PAPA (λex = 280 nm) were 81.46%~95.95% (293K), 80.50%~95.49% (308K) and 79.77%~95.12% (318K). There was a linear relationship between ΔF and the concentrations of CEF in the range of 6.0×10-6~7.0×10-5 mol/L. And the correlation coefficient of the linear curve was 0.9973.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Mechanism of Interaction Between Cefepime and Papain by Spectroscopic Method
    AU  - Li Chen
    AU  - Baosheng Liu
    AU  - Chundan Wang
    AU  - Lihua Ma
    AU  - Hongcai Zhang
    AU  - Xu Guo
    Y1  - 2018/03/19
    PY  - 2018
    N1  - https://doi.org/10.11648/j.jmpt.20180401.16
    DO  - 10.11648/j.jmpt.20180401.16
    T2  - Journal of Photonic Materials and Technology
    JF  - Journal of Photonic Materials and Technology
    JO  - Journal of Photonic Materials and Technology
    SP  - 33
    EP  - 38
    PB  - Science Publishing Group
    SN  - 2469-8431
    UR  - https://doi.org/10.11648/j.jmpt.20180401.16
    AB  - The reaction mechanism of cefepime (CEF) with papain (PAPA) was investigated by both fluorescence quenching and synchronous fluorescence spectroscopy in different temperature (293, 308 and 318 K). The results demonstrated that the interaction between CEF and PAPA was taking place via static quenching. Thermodynamic parameters revealed that electrostatic interaction played major roles in the interaction. The order of magnitudes of binding constant is 104, and the number of binding site in the system was closed to 1. The binding rates of CEF to PAPA (λex = 280 nm) were 81.46%~95.95% (293K), 80.50%~95.49% (308K) and 79.77%~95.12% (318K). There was a linear relationship between ΔF and the concentrations of CEF in the range of 6.0×10-6~7.0×10-5 mol/L. And the correlation coefficient of the linear curve was 0.9973.
    VL  - 4
    IS  - 1
    ER  - 

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Author Information
  • College of Chemistry & Environmental Science, Hebei University, Baoding, P. R. China

  • College of Chemistry & Environmental Science, Hebei University, Baoding, P. R. China

  • College of Chemistry & Environmental Science, Hebei University, Baoding, P. R. China

  • College of Chemistry & Environmental Science, Hebei University, Baoding, P. R. China

  • College of Chemistry & Environmental Science, Hebei University, Baoding, P. R. China

  • College of Chemistry & Environmental Science, Hebei University, Baoding, P. R. China

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