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Antibiotic Susceptibility Profile of Bacteria Isolated from Door Handles of University of Benin Teaching Hospital, Benin City, Edo State, Nigeria

Received: 6 January 2018     Accepted: 16 February 2018     Published: 23 March 2018
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Abstract

In recent times, surfaces of hospital environment, constantly exposed to activities of patients and health-care workers, have been identified as potential routes for horizontal dissemination of drug resistant microorganisms of public health significance. This study was conducted to investigate the antibiotic resistance profile of bacteria isolated from samples collected from door handles of various units in University of Benin Teaching Hospital (UBTH), Benin City, Edo State, Nigeria. Out of the 66 door handles sampled and analysed bacteriologically, 68 predominant bacteria distributed among five (5) species of Escherichia coli (30.9%), Staphylococcusaureus (26.5%), Pseudomonas aeruginosa (17.6%), Streptococcus pneumonia (13.2%) and Bacillus subtilis (11.8%) were isolated. Kirby Bauer techniques of disk diffusion antibiotic susceptibility test on the isolates using 9 commercial antibiotics according to Clinical Laboratory Standards Institute (CLSI) procedure showed resistance of P. aeruginosa to five (5) drugs (Sulfamethoxazole-Trimethoprim, Streptomycin, Ampicillin, Cephalexin and Nalidixic acid), E. coli to five (5) drugs (Ofloxacin, Sulfamethoxazole-Trimethoprim, Ampicillin, Cephalexin and Nalidixic acid), S. pneumoniaeto two drugs (Ampicilin and Nalidixic acid) and S aureus to only Nalidixic acid. Agarose gel electrophoresis carried out on their plasmid DNA revealed detectable fragments in P. aeruginosa and E. coli. After plasmid curing, the isolates became sensitive to Streptomycin, Cephalexin,Ofloxacin, Sulfamethoxazole-Trimethoprim, Ampicillin and Ciprofloxacin. Findings from this study suggest the presence of plasmid mediated multi-drug resistant P. aeruginosa and E. coli on surfaces of hospital door handles. Hence, public education on proper personal hygiene and disinfection techniques are recommended in hospitals.

Published in Journal of Health and Environmental Research (Volume 4, Issue 1)
DOI 10.11648/j.jher.20180401.15
Page(s) 35-41
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Antibiotics, Door Handles, Plasmid, Bacteria

References
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Cite This Article
  • APA Style

    Augustine Brian Odigie, Frederick Osaro Ekhaise, Paul Ikechukwu Orjiakor, Eze Chibuzor Nwadibe, Odeyemi Adebowale Toba, et al. (2018). Antibiotic Susceptibility Profile of Bacteria Isolated from Door Handles of University of Benin Teaching Hospital, Benin City, Edo State, Nigeria. Journal of Health and Environmental Research, 4(1), 35-41. https://doi.org/10.11648/j.jher.20180401.15

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    ACS Style

    Augustine Brian Odigie; Frederick Osaro Ekhaise; Paul Ikechukwu Orjiakor; Eze Chibuzor Nwadibe; Odeyemi Adebowale Toba, et al. Antibiotic Susceptibility Profile of Bacteria Isolated from Door Handles of University of Benin Teaching Hospital, Benin City, Edo State, Nigeria. J. Health Environ. Res. 2018, 4(1), 35-41. doi: 10.11648/j.jher.20180401.15

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    AMA Style

    Augustine Brian Odigie, Frederick Osaro Ekhaise, Paul Ikechukwu Orjiakor, Eze Chibuzor Nwadibe, Odeyemi Adebowale Toba, et al. Antibiotic Susceptibility Profile of Bacteria Isolated from Door Handles of University of Benin Teaching Hospital, Benin City, Edo State, Nigeria. J Health Environ Res. 2018;4(1):35-41. doi: 10.11648/j.jher.20180401.15

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  • @article{10.11648/j.jher.20180401.15,
      author = {Augustine Brian Odigie and Frederick Osaro Ekhaise and Paul Ikechukwu Orjiakor and Eze Chibuzor Nwadibe and Odeyemi Adebowale Toba and Odoh Chuks Kenneth},
      title = {Antibiotic Susceptibility Profile of Bacteria Isolated from Door Handles of University of Benin Teaching Hospital, Benin City, Edo State, Nigeria},
      journal = {Journal of Health and Environmental Research},
      volume = {4},
      number = {1},
      pages = {35-41},
      doi = {10.11648/j.jher.20180401.15},
      url = {https://doi.org/10.11648/j.jher.20180401.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jher.20180401.15},
      abstract = {In recent times, surfaces of hospital environment, constantly exposed to activities of patients and health-care workers, have been identified as potential routes for horizontal dissemination of drug resistant microorganisms of public health significance. This study was conducted to investigate the antibiotic resistance profile of bacteria isolated from samples collected from door handles of various units in University of Benin Teaching Hospital (UBTH), Benin City, Edo State, Nigeria. Out of the 66 door handles sampled and analysed bacteriologically, 68 predominant bacteria distributed among five (5) species of Escherichia coli (30.9%), Staphylococcusaureus (26.5%), Pseudomonas aeruginosa (17.6%), Streptococcus pneumonia (13.2%) and Bacillus subtilis (11.8%) were isolated. Kirby Bauer techniques of disk diffusion antibiotic susceptibility test on the isolates using 9 commercial antibiotics according to Clinical Laboratory Standards Institute (CLSI) procedure showed resistance of P. aeruginosa to five (5) drugs (Sulfamethoxazole-Trimethoprim, Streptomycin, Ampicillin, Cephalexin and Nalidixic acid), E. coli to five (5) drugs (Ofloxacin, Sulfamethoxazole-Trimethoprim, Ampicillin, Cephalexin and Nalidixic acid), S. pneumoniaeto two drugs (Ampicilin and Nalidixic acid) and S aureus to only Nalidixic acid. Agarose gel electrophoresis carried out on their plasmid DNA revealed detectable fragments in P. aeruginosa and E. coli. After plasmid curing, the isolates became sensitive to Streptomycin, Cephalexin,Ofloxacin, Sulfamethoxazole-Trimethoprim, Ampicillin and Ciprofloxacin. Findings from this study suggest the presence of plasmid mediated multi-drug resistant P. aeruginosa and E. coli on surfaces of hospital door handles. Hence, public education on proper personal hygiene and disinfection techniques are recommended in hospitals.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Antibiotic Susceptibility Profile of Bacteria Isolated from Door Handles of University of Benin Teaching Hospital, Benin City, Edo State, Nigeria
    AU  - Augustine Brian Odigie
    AU  - Frederick Osaro Ekhaise
    AU  - Paul Ikechukwu Orjiakor
    AU  - Eze Chibuzor Nwadibe
    AU  - Odeyemi Adebowale Toba
    AU  - Odoh Chuks Kenneth
    Y1  - 2018/03/23
    PY  - 2018
    N1  - https://doi.org/10.11648/j.jher.20180401.15
    DO  - 10.11648/j.jher.20180401.15
    T2  - Journal of Health and Environmental Research
    JF  - Journal of Health and Environmental Research
    JO  - Journal of Health and Environmental Research
    SP  - 35
    EP  - 41
    PB  - Science Publishing Group
    SN  - 2472-3592
    UR  - https://doi.org/10.11648/j.jher.20180401.15
    AB  - In recent times, surfaces of hospital environment, constantly exposed to activities of patients and health-care workers, have been identified as potential routes for horizontal dissemination of drug resistant microorganisms of public health significance. This study was conducted to investigate the antibiotic resistance profile of bacteria isolated from samples collected from door handles of various units in University of Benin Teaching Hospital (UBTH), Benin City, Edo State, Nigeria. Out of the 66 door handles sampled and analysed bacteriologically, 68 predominant bacteria distributed among five (5) species of Escherichia coli (30.9%), Staphylococcusaureus (26.5%), Pseudomonas aeruginosa (17.6%), Streptococcus pneumonia (13.2%) and Bacillus subtilis (11.8%) were isolated. Kirby Bauer techniques of disk diffusion antibiotic susceptibility test on the isolates using 9 commercial antibiotics according to Clinical Laboratory Standards Institute (CLSI) procedure showed resistance of P. aeruginosa to five (5) drugs (Sulfamethoxazole-Trimethoprim, Streptomycin, Ampicillin, Cephalexin and Nalidixic acid), E. coli to five (5) drugs (Ofloxacin, Sulfamethoxazole-Trimethoprim, Ampicillin, Cephalexin and Nalidixic acid), S. pneumoniaeto two drugs (Ampicilin and Nalidixic acid) and S aureus to only Nalidixic acid. Agarose gel electrophoresis carried out on their plasmid DNA revealed detectable fragments in P. aeruginosa and E. coli. After plasmid curing, the isolates became sensitive to Streptomycin, Cephalexin,Ofloxacin, Sulfamethoxazole-Trimethoprim, Ampicillin and Ciprofloxacin. Findings from this study suggest the presence of plasmid mediated multi-drug resistant P. aeruginosa and E. coli on surfaces of hospital door handles. Hence, public education on proper personal hygiene and disinfection techniques are recommended in hospitals.
    VL  - 4
    IS  - 1
    ER  - 

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Author Information
  • Department of Microbiology, Faculty of Life Sciences, University of Benin, Edo State, Nigeria

  • Department of Microbiology, Faculty of Life Sciences, University of Benin, Edo State, Nigeria

  • Department of Microbiology, Faculty of Sciences, Ekiti State University, Ekiti State, Nigeria

  • Department of Microbiology, Faculty of Biological Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria

  • Department of Microbiology, Faculty of Sciences, Ekiti State University, Ekiti State, Nigeria

  • Department of Microbiology, Faculty of Biological Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria

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