In recent times, surfaces of hospital environment, constantly exposed to activities of patients and health-care workers, have been identified as potential routes for horizontal dissemination of drug resistant microorganisms of public health significance. This study was conducted to investigate the antibiotic resistance profile of bacteria isolated from samples collected from door handles of various units in University of Benin Teaching Hospital (UBTH), Benin City, Edo State, Nigeria. Out of the 66 door handles sampled and analysed bacteriologically, 68 predominant bacteria distributed among five (5) species of Escherichia coli (30.9%), Staphylococcusaureus (26.5%), Pseudomonas aeruginosa (17.6%), Streptococcus pneumonia (13.2%) and Bacillus subtilis (11.8%) were isolated. Kirby Bauer techniques of disk diffusion antibiotic susceptibility test on the isolates using 9 commercial antibiotics according to Clinical Laboratory Standards Institute (CLSI) procedure showed resistance of P. aeruginosa to five (5) drugs (Sulfamethoxazole-Trimethoprim, Streptomycin, Ampicillin, Cephalexin and Nalidixic acid), E. coli to five (5) drugs (Ofloxacin, Sulfamethoxazole-Trimethoprim, Ampicillin, Cephalexin and Nalidixic acid), S. pneumoniaeto two drugs (Ampicilin and Nalidixic acid) and S aureus to only Nalidixic acid. Agarose gel electrophoresis carried out on their plasmid DNA revealed detectable fragments in P. aeruginosa and E. coli. After plasmid curing, the isolates became sensitive to Streptomycin, Cephalexin,Ofloxacin, Sulfamethoxazole-Trimethoprim, Ampicillin and Ciprofloxacin. Findings from this study suggest the presence of plasmid mediated multi-drug resistant P. aeruginosa and E. coli on surfaces of hospital door handles. Hence, public education on proper personal hygiene and disinfection techniques are recommended in hospitals.
| Published in | Journal of Health and Environmental Research (Volume 4, Issue 1) |
| DOI | 10.11648/j.jher.20180401.15 |
| Page(s) | 35-41 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2018. Published by Science Publishing Group |
Antibiotics, Door Handles, Plasmid, Bacteria
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APA Style
Augustine Brian Odigie, Frederick Osaro Ekhaise, Paul Ikechukwu Orjiakor, Eze Chibuzor Nwadibe, Odeyemi Adebowale Toba, et al. (2018). Antibiotic Susceptibility Profile of Bacteria Isolated from Door Handles of University of Benin Teaching Hospital, Benin City, Edo State, Nigeria. Journal of Health and Environmental Research, 4(1), 35-41. https://doi.org/10.11648/j.jher.20180401.15
ACS Style
Augustine Brian Odigie; Frederick Osaro Ekhaise; Paul Ikechukwu Orjiakor; Eze Chibuzor Nwadibe; Odeyemi Adebowale Toba, et al. Antibiotic Susceptibility Profile of Bacteria Isolated from Door Handles of University of Benin Teaching Hospital, Benin City, Edo State, Nigeria. J. Health Environ. Res. 2018, 4(1), 35-41. doi: 10.11648/j.jher.20180401.15
AMA Style
Augustine Brian Odigie, Frederick Osaro Ekhaise, Paul Ikechukwu Orjiakor, Eze Chibuzor Nwadibe, Odeyemi Adebowale Toba, et al. Antibiotic Susceptibility Profile of Bacteria Isolated from Door Handles of University of Benin Teaching Hospital, Benin City, Edo State, Nigeria. J Health Environ Res. 2018;4(1):35-41. doi: 10.11648/j.jher.20180401.15
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author = {Augustine Brian Odigie and Frederick Osaro Ekhaise and Paul Ikechukwu Orjiakor and Eze Chibuzor Nwadibe and Odeyemi Adebowale Toba and Odoh Chuks Kenneth},
title = {Antibiotic Susceptibility Profile of Bacteria Isolated from Door Handles of University of Benin Teaching Hospital, Benin City, Edo State, Nigeria},
journal = {Journal of Health and Environmental Research},
volume = {4},
number = {1},
pages = {35-41},
doi = {10.11648/j.jher.20180401.15},
url = {https://doi.org/10.11648/j.jher.20180401.15},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jher.20180401.15},
abstract = {In recent times, surfaces of hospital environment, constantly exposed to activities of patients and health-care workers, have been identified as potential routes for horizontal dissemination of drug resistant microorganisms of public health significance. This study was conducted to investigate the antibiotic resistance profile of bacteria isolated from samples collected from door handles of various units in University of Benin Teaching Hospital (UBTH), Benin City, Edo State, Nigeria. Out of the 66 door handles sampled and analysed bacteriologically, 68 predominant bacteria distributed among five (5) species of Escherichia coli (30.9%), Staphylococcusaureus (26.5%), Pseudomonas aeruginosa (17.6%), Streptococcus pneumonia (13.2%) and Bacillus subtilis (11.8%) were isolated. Kirby Bauer techniques of disk diffusion antibiotic susceptibility test on the isolates using 9 commercial antibiotics according to Clinical Laboratory Standards Institute (CLSI) procedure showed resistance of P. aeruginosa to five (5) drugs (Sulfamethoxazole-Trimethoprim, Streptomycin, Ampicillin, Cephalexin and Nalidixic acid), E. coli to five (5) drugs (Ofloxacin, Sulfamethoxazole-Trimethoprim, Ampicillin, Cephalexin and Nalidixic acid), S. pneumoniaeto two drugs (Ampicilin and Nalidixic acid) and S aureus to only Nalidixic acid. Agarose gel electrophoresis carried out on their plasmid DNA revealed detectable fragments in P. aeruginosa and E. coli. After plasmid curing, the isolates became sensitive to Streptomycin, Cephalexin,Ofloxacin, Sulfamethoxazole-Trimethoprim, Ampicillin and Ciprofloxacin. Findings from this study suggest the presence of plasmid mediated multi-drug resistant P. aeruginosa and E. coli on surfaces of hospital door handles. Hence, public education on proper personal hygiene and disinfection techniques are recommended in hospitals.},
year = {2018}
}
TY - JOUR T1 - Antibiotic Susceptibility Profile of Bacteria Isolated from Door Handles of University of Benin Teaching Hospital, Benin City, Edo State, Nigeria AU - Augustine Brian Odigie AU - Frederick Osaro Ekhaise AU - Paul Ikechukwu Orjiakor AU - Eze Chibuzor Nwadibe AU - Odeyemi Adebowale Toba AU - Odoh Chuks Kenneth Y1 - 2018/03/23 PY - 2018 N1 - https://doi.org/10.11648/j.jher.20180401.15 DO - 10.11648/j.jher.20180401.15 T2 - Journal of Health and Environmental Research JF - Journal of Health and Environmental Research JO - Journal of Health and Environmental Research SP - 35 EP - 41 PB - Science Publishing Group SN - 2472-3592 UR - https://doi.org/10.11648/j.jher.20180401.15 AB - In recent times, surfaces of hospital environment, constantly exposed to activities of patients and health-care workers, have been identified as potential routes for horizontal dissemination of drug resistant microorganisms of public health significance. This study was conducted to investigate the antibiotic resistance profile of bacteria isolated from samples collected from door handles of various units in University of Benin Teaching Hospital (UBTH), Benin City, Edo State, Nigeria. Out of the 66 door handles sampled and analysed bacteriologically, 68 predominant bacteria distributed among five (5) species of Escherichia coli (30.9%), Staphylococcusaureus (26.5%), Pseudomonas aeruginosa (17.6%), Streptococcus pneumonia (13.2%) and Bacillus subtilis (11.8%) were isolated. Kirby Bauer techniques of disk diffusion antibiotic susceptibility test on the isolates using 9 commercial antibiotics according to Clinical Laboratory Standards Institute (CLSI) procedure showed resistance of P. aeruginosa to five (5) drugs (Sulfamethoxazole-Trimethoprim, Streptomycin, Ampicillin, Cephalexin and Nalidixic acid), E. coli to five (5) drugs (Ofloxacin, Sulfamethoxazole-Trimethoprim, Ampicillin, Cephalexin and Nalidixic acid), S. pneumoniaeto two drugs (Ampicilin and Nalidixic acid) and S aureus to only Nalidixic acid. Agarose gel electrophoresis carried out on their plasmid DNA revealed detectable fragments in P. aeruginosa and E. coli. After plasmid curing, the isolates became sensitive to Streptomycin, Cephalexin,Ofloxacin, Sulfamethoxazole-Trimethoprim, Ampicillin and Ciprofloxacin. Findings from this study suggest the presence of plasmid mediated multi-drug resistant P. aeruginosa and E. coli on surfaces of hospital door handles. Hence, public education on proper personal hygiene and disinfection techniques are recommended in hospitals. VL - 4 IS - 1 ER -
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