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Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum In Vitro by Thiamin and Quinine

Received: 5 July 2018     Accepted: 16 July 2018     Published: 22 October 2018
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Abstract

Previously, the effects of several bitter substances have been investigated in the contraction of the murine jejunum and ileum, reporting that these independently suppress the rhythm generation of the interstitial cells of Cajal. Recently, it was reported that thiamin, which binds to a bitter taste receptor, modifies the early phase of the ileum contraction, whereas the physiological effects on the rhythm and amplitude of jejunum and ileum contractions remain unclear. In this study, it was investigated the physiological effects of thiamin and quinine on the in vitro contraction of the murine jejunum and ileum using mice for all experiments. the periodic contraction of the jejunum was observed before the administration of acetylcholine (Ach) and other substances, and the tonic amplitudes induced by the substances. These bitter substances variably affect the Ach-induced rhythmic contraction of the jejunum and ileum in vitro. In addition, quinine hydrochloride (Qui) and thiamin hydrochloride (Thi) variably affect the Ach-induced rhythmic contraction of the jejunum and ileum in vitro. Both Qui and Thi markedly increase the rhythmic contraction in the jejunum. Although Thi does not change the rhythmic contraction in the ileum, it gradually reduces the amplitude in the jejunum. Conversely, Qui gradually reduces the amplitude and almost inhibits the contraction in the jejunum. Furthermore, an antagonist of the adrenalin-beta3 receptor, SR59230A, enhances the Qui-induced inhibition of the contraction in the jejunum.

Published in Journal of Food and Nutrition Sciences (Volume 6, Issue 5)
DOI 10.11648/j.jfns.20180605.11
Page(s) 115-122
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Thiamin, Quinine, Small Intestine, Mouse

References
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Cite This Article
  • APA Style

    Atsuko Yamashita, Nana Shimamoto, Kyoko Morita, Hasumi Sugiyama, Shiho Tadakuma, et al. (2018). Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum In Vitro by Thiamin and Quinine. Journal of Food and Nutrition Sciences, 6(5), 115-122. https://doi.org/10.11648/j.jfns.20180605.11

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    ACS Style

    Atsuko Yamashita; Nana Shimamoto; Kyoko Morita; Hasumi Sugiyama; Shiho Tadakuma, et al. Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum In Vitro by Thiamin and Quinine. J. Food Nutr. Sci. 2018, 6(5), 115-122. doi: 10.11648/j.jfns.20180605.11

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    AMA Style

    Atsuko Yamashita, Nana Shimamoto, Kyoko Morita, Hasumi Sugiyama, Shiho Tadakuma, et al. Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum In Vitro by Thiamin and Quinine. J Food Nutr Sci. 2018;6(5):115-122. doi: 10.11648/j.jfns.20180605.11

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  • @article{10.11648/j.jfns.20180605.11,
      author = {Atsuko Yamashita and Nana Shimamoto and Kyoko Morita and Hasumi Sugiyama and Shiho Tadakuma and Maki Kato and Mari Kimoto and Kazuo Toda and Masato Ota},
      title = {Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum In Vitro by Thiamin and Quinine},
      journal = {Journal of Food and Nutrition Sciences},
      volume = {6},
      number = {5},
      pages = {115-122},
      doi = {10.11648/j.jfns.20180605.11},
      url = {https://doi.org/10.11648/j.jfns.20180605.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jfns.20180605.11},
      abstract = {Previously, the effects of several bitter substances have been investigated in the contraction of the murine jejunum and ileum, reporting that these independently suppress the rhythm generation of the interstitial cells of Cajal. Recently, it was reported that thiamin, which binds to a bitter taste receptor, modifies the early phase of the ileum contraction, whereas the physiological effects on the rhythm and amplitude of jejunum and ileum contractions remain unclear. In this study, it was investigated the physiological effects of thiamin and quinine on the in vitro contraction of the murine jejunum and ileum using mice for all experiments. the periodic contraction of the jejunum was observed before the administration of acetylcholine (Ach) and other substances, and the tonic amplitudes induced by the substances. These bitter substances variably affect the Ach-induced rhythmic contraction of the jejunum and ileum in vitro. In addition, quinine hydrochloride (Qui) and thiamin hydrochloride (Thi) variably affect the Ach-induced rhythmic contraction of the jejunum and ileum in vitro. Both Qui and Thi markedly increase the rhythmic contraction in the jejunum. Although Thi does not change the rhythmic contraction in the ileum, it gradually reduces the amplitude in the jejunum. Conversely, Qui gradually reduces the amplitude and almost inhibits the contraction in the jejunum. Furthermore, an antagonist of the adrenalin-beta3 receptor, SR59230A, enhances the Qui-induced inhibition of the contraction in the jejunum.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum In Vitro by Thiamin and Quinine
    AU  - Atsuko Yamashita
    AU  - Nana Shimamoto
    AU  - Kyoko Morita
    AU  - Hasumi Sugiyama
    AU  - Shiho Tadakuma
    AU  - Maki Kato
    AU  - Mari Kimoto
    AU  - Kazuo Toda
    AU  - Masato Ota
    Y1  - 2018/10/22
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    DO  - 10.11648/j.jfns.20180605.11
    T2  - Journal of Food and Nutrition Sciences
    JF  - Journal of Food and Nutrition Sciences
    JO  - Journal of Food and Nutrition Sciences
    SP  - 115
    EP  - 122
    PB  - Science Publishing Group
    SN  - 2330-7293
    UR  - https://doi.org/10.11648/j.jfns.20180605.11
    AB  - Previously, the effects of several bitter substances have been investigated in the contraction of the murine jejunum and ileum, reporting that these independently suppress the rhythm generation of the interstitial cells of Cajal. Recently, it was reported that thiamin, which binds to a bitter taste receptor, modifies the early phase of the ileum contraction, whereas the physiological effects on the rhythm and amplitude of jejunum and ileum contractions remain unclear. In this study, it was investigated the physiological effects of thiamin and quinine on the in vitro contraction of the murine jejunum and ileum using mice for all experiments. the periodic contraction of the jejunum was observed before the administration of acetylcholine (Ach) and other substances, and the tonic amplitudes induced by the substances. These bitter substances variably affect the Ach-induced rhythmic contraction of the jejunum and ileum in vitro. In addition, quinine hydrochloride (Qui) and thiamin hydrochloride (Thi) variably affect the Ach-induced rhythmic contraction of the jejunum and ileum in vitro. Both Qui and Thi markedly increase the rhythmic contraction in the jejunum. Although Thi does not change the rhythmic contraction in the ileum, it gradually reduces the amplitude in the jejunum. Conversely, Qui gradually reduces the amplitude and almost inhibits the contraction in the jejunum. Furthermore, an antagonist of the adrenalin-beta3 receptor, SR59230A, enhances the Qui-induced inhibition of the contraction in the jejunum.
    VL  - 6
    IS  - 5
    ER  - 

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Author Information
  • Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Tokyo, Japan

  • Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Tokyo, Japan

  • Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Tokyo, Japan

  • Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Tokyo, Japan

  • Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Tokyo, Japan

  • Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Tokyo, Japan

  • Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Tokyo, Japan

  • Integrative Sensory Physiology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan

  • Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Tokyo, Japan

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