Journal of Drug Design and Medicinal Chemistry

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Free Radical Scavenging Activity of Alkaloid and Flavonoid Fractions of Kalanchoe pinnata Leaves

Received: May 12, 2023    Accepted: May 29, 2023    Published: Jun. 10, 2023
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Abstract

Kalanchoe pinnata is an important medicinal plant in Nigeria with numerous pharmacological activities. The present study investigates the antioxidant activity of alkaloid and flavonoid fractions of Kalanchoe pinnata leaves. Proximate and phytochemical analyses of the plant were conducted followed by evaluation of the antioxidant activity of the alkaloid and flavonoid fractions using 1,1-diphenyl-2-picryl-hydrazyl, 2,2’-azinobis(3-ethylbenzothiazolline)-6-sulfonic acid, phosphomolybdenum, cupric ion reducing antioxidant capacity, ferric reducing antioxidant power and nitric oxide inhibition assays. The proximate composition of Kalanchoe pinnata leaves confirmed its edibility particularly in respect of moisture (86.73%) and carbohydrate (7.05%) content. The results revealed the presence of phenol (12.50 mg TAE/g), flavonoid (18.10 mg QE/g), flavanol (17.70 mg CE/g), alkaloid (26.98 mg/g), tannin (4.03 mg/g) and saponin (3.69 mg/g). Antioxidant assays indicated that both fractions exhibited antioxidant activity that is comparable to ascorbic acid and rutin with nitric oxide having the highest scavenging potential of 98.97% inhibition. GC-MS analysis of the alkaloid and flavonoid fractions revealed the presence of 18 and 22 compounds respectively. The antioxidant activity of Kalanchoe pinnata may be attributed to 5-hydroxymethyl furfural and oleic acid detected in the alkaloid and flavonoid fractions respectively. Overall, the data generated form this study portrayed Kalanchoe pinnata as a potential antioxidant agent.

DOI 10.11648/j.jddmc.20230901.12
Published in Journal of Drug Design and Medicinal Chemistry ( Volume 9, Issue 1, March 2023 )
Page(s) 7-15
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Free Radicals, Oxidative Stress, Alkaloid, Flavonoid, Antioxidants

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    Taofik Olatunde Uthman, Rabiat Yetunde Salman, Ismail Abubakar Lamorde. (2023). Free Radical Scavenging Activity of Alkaloid and Flavonoid Fractions of Kalanchoe pinnata Leaves. Journal of Drug Design and Medicinal Chemistry, 9(1), 7-15. https://doi.org/10.11648/j.jddmc.20230901.12

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    ACS Style

    Taofik Olatunde Uthman; Rabiat Yetunde Salman; Ismail Abubakar Lamorde. Free Radical Scavenging Activity of Alkaloid and Flavonoid Fractions of Kalanchoe pinnata Leaves. J. Drug Des. Med. Chem. 2023, 9(1), 7-15. doi: 10.11648/j.jddmc.20230901.12

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    AMA Style

    Taofik Olatunde Uthman, Rabiat Yetunde Salman, Ismail Abubakar Lamorde. Free Radical Scavenging Activity of Alkaloid and Flavonoid Fractions of Kalanchoe pinnata Leaves. J Drug Des Med Chem. 2023;9(1):7-15. doi: 10.11648/j.jddmc.20230901.12

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  • @article{10.11648/j.jddmc.20230901.12,
      author = {Taofik Olatunde Uthman and Rabiat Yetunde Salman and Ismail Abubakar Lamorde},
      title = {Free Radical Scavenging Activity of Alkaloid and Flavonoid Fractions of Kalanchoe pinnata Leaves},
      journal = {Journal of Drug Design and Medicinal Chemistry},
      volume = {9},
      number = {1},
      pages = {7-15},
      doi = {10.11648/j.jddmc.20230901.12},
      url = {https://doi.org/10.11648/j.jddmc.20230901.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.jddmc.20230901.12},
      abstract = {Kalanchoe pinnata is an important medicinal plant in Nigeria with numerous pharmacological activities. The present study investigates the antioxidant activity of alkaloid and flavonoid fractions of Kalanchoe pinnata leaves. Proximate and phytochemical analyses of the plant were conducted followed by evaluation of the antioxidant activity of the alkaloid and flavonoid fractions using 1,1-diphenyl-2-picryl-hydrazyl, 2,2’-azinobis(3-ethylbenzothiazolline)-6-sulfonic acid, phosphomolybdenum, cupric ion reducing antioxidant capacity, ferric reducing antioxidant power and nitric oxide inhibition assays. The proximate composition of Kalanchoe pinnata leaves confirmed its edibility particularly in respect of moisture (86.73%) and carbohydrate (7.05%) content. The results revealed the presence of phenol (12.50 mg TAE/g), flavonoid (18.10 mg QE/g), flavanol (17.70 mg CE/g), alkaloid (26.98 mg/g), tannin (4.03 mg/g) and saponin (3.69 mg/g). Antioxidant assays indicated that both fractions exhibited antioxidant activity that is comparable to ascorbic acid and rutin with nitric oxide having the highest scavenging potential of 98.97% inhibition. GC-MS analysis of the alkaloid and flavonoid fractions revealed the presence of 18 and 22 compounds respectively. The antioxidant activity of Kalanchoe pinnata may be attributed to 5-hydroxymethyl furfural and oleic acid detected in the alkaloid and flavonoid fractions respectively. Overall, the data generated form this study portrayed Kalanchoe pinnata as a potential antioxidant agent.},
     year = {2023}
    }
    

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  • TY  - JOUR
    T1  - Free Radical Scavenging Activity of Alkaloid and Flavonoid Fractions of Kalanchoe pinnata Leaves
    AU  - Taofik Olatunde Uthman
    AU  - Rabiat Yetunde Salman
    AU  - Ismail Abubakar Lamorde
    Y1  - 2023/06/10
    PY  - 2023
    N1  - https://doi.org/10.11648/j.jddmc.20230901.12
    DO  - 10.11648/j.jddmc.20230901.12
    T2  - Journal of Drug Design and Medicinal Chemistry
    JF  - Journal of Drug Design and Medicinal Chemistry
    JO  - Journal of Drug Design and Medicinal Chemistry
    SP  - 7
    EP  - 15
    PB  - Science Publishing Group
    SN  - 2472-3576
    UR  - https://doi.org/10.11648/j.jddmc.20230901.12
    AB  - Kalanchoe pinnata is an important medicinal plant in Nigeria with numerous pharmacological activities. The present study investigates the antioxidant activity of alkaloid and flavonoid fractions of Kalanchoe pinnata leaves. Proximate and phytochemical analyses of the plant were conducted followed by evaluation of the antioxidant activity of the alkaloid and flavonoid fractions using 1,1-diphenyl-2-picryl-hydrazyl, 2,2’-azinobis(3-ethylbenzothiazolline)-6-sulfonic acid, phosphomolybdenum, cupric ion reducing antioxidant capacity, ferric reducing antioxidant power and nitric oxide inhibition assays. The proximate composition of Kalanchoe pinnata leaves confirmed its edibility particularly in respect of moisture (86.73%) and carbohydrate (7.05%) content. The results revealed the presence of phenol (12.50 mg TAE/g), flavonoid (18.10 mg QE/g), flavanol (17.70 mg CE/g), alkaloid (26.98 mg/g), tannin (4.03 mg/g) and saponin (3.69 mg/g). Antioxidant assays indicated that both fractions exhibited antioxidant activity that is comparable to ascorbic acid and rutin with nitric oxide having the highest scavenging potential of 98.97% inhibition. GC-MS analysis of the alkaloid and flavonoid fractions revealed the presence of 18 and 22 compounds respectively. The antioxidant activity of Kalanchoe pinnata may be attributed to 5-hydroxymethyl furfural and oleic acid detected in the alkaloid and flavonoid fractions respectively. Overall, the data generated form this study portrayed Kalanchoe pinnata as a potential antioxidant agent.
    VL  - 9
    IS  - 1
    ER  - 

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Author Information
  • Department of Biochemistry and Chemistry, Faculty of Natural and Applied Sciences, Nile University of Nigeria, Abuja, Nigeria

  • Department of Biochemistry and Chemistry, Faculty of Natural and Applied Sciences, Nile University of Nigeria, Abuja, Nigeria

  • Department of Biochemistry and Chemistry, Faculty of Natural and Applied Sciences, Nile University of Nigeria, Abuja, Nigeria

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