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Isolation of Mycotoxigenic Fungi, Detection and Quantification of Ochratoxin A from Coffee (Coffea arabica L.)

Received: 30 December 2020     Accepted: 12 January 2021     Published: 22 January 2021
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Abstract

Introduction. Successful prevention of mother-to-child transmission (PMTCT) of the human immunodeficiency virus (HIV) requires early diagnosis, consistent access to antiretroviral therapy (ART), and regular health care during and after pregnancy. This study assessed the determinants of early HIV diagnosis by children born to HIV-positive (HIV+) mothers in the Kongo Central Province, Democratic Republic of the Congo (DRC). Methodology. Data from 230 HIV+ mothers screened under the PMTCT program between July 2015 and December 2017 were extracted from the databases of 31 Health Zones (HZ) of Kongo Central province. Data detailing laboratory and anthropometric findings, morbidity, and mortality were collected from each exposed child. The determinants of inaccessibility to early diagnosis were identified using logistic regression. Results. The mothers' mean age was 32.4 years, and 90.9% were on ART; 68.8% of children were older than 12 months at diagnosis. Diagnosis during the first 6 months of the child’s life was critical. Malnutrition was found in almost 90% of the children. Determinants of non-access to an early diagnosis were mothers who attained a primary education level and did not disclose their HIV status. Conclusion. All possible interventions should be considered to prevent mother-to-child HIV transmission. To ensure a child’s survival to their first birthday, early diagnosis should be performed soon after birth to an HIV+ mother and ART should be initiated.

Published in Journal of Drug Design and Medicinal Chemistry (Volume 6, Issue 4)
DOI 10.11648/j.jddmc.20200604.12
Page(s) 47-52
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Coffee, Ethiopia, Mycotoxigenic Fungi, Ochratoxin A

References
[1] Davis, A. P., Gole, T. W., Baena, S. and Moat, J., 2012. The impact of climate change on indigenous arabica coffee (Coffea arabica): predicting future trends and identifying priorities. PloS one, 7 (11). https://journals.plos.org/plosone/article/file?type=printable&id=10.1371/journal.pone.0047981.
[2] Esquivel, P. and Jiménez, V. M., 2012. Functional properties of coffee and coffee by-products. Food Research International, 46 (2), pp. 488-495. https://www.sciencedirect.com/science/article/abs/pii/S0963996911003449.
[3] International Coffee Genomics Network (ICGN). (2017). Report 10thCoffee Genomics Workshop held at the XXV Plant and Animal Genome (PAG) Meeting San Diego, California. January 14-18.
[4] USDA, N. (2012). The plants database. National plant data team, Greensboro, NC 27401-4901 USA.
[5] Foreign Agricultural Service (FAS). (2019). Coffee Annual Report. GAIN Report: ET1904.
[6] ICO (International Coffee Organization). 2016. Historical Data on the Global Coffee Trade. Available on http://www.ico.org/new storical.asp (2016). Accessed date on January, 2020. http://www.ico.org/new_historical.asp.
[7] Malaker, P. K., Mian, I. H., Bhuiyan, K. A., Akanda, A. M. and Reza, M. M. A. (2008). Effect of storage containers and time on seed quality of wheat. Bangladesh Journal of Agricultural Research, 33: 469-477. https://www.banglajol.info/index.php/BJAR/article/view/1606.
[8] Taniwaki, M. H., Pitt, J. I., Teixeira, A. A. and Iamanaka, B. T. (2003). The source of ochratoxin A in Brazilian coffee and its formation in relation to processing methods. Int J Food Microbiol. 82: 173-179. https://www.sciencedirect.com/science/article/abs/pii/S0168160502003100.
[9] Almeida, A. P.; Alaburda, J.; Shundo, L.; Ruvieri, V.; Navas, S. A.; Lamardo, L. C. A. M. (2007). Ochratoxin A in Brazilian instant coffee. Braz. Microb, 3: 300-303. https://doi.org/10.1590/S1517-83822007000200022.
[10] Amézqueta, S., González-Peñas, E., Murillo-Arbizu, M. and de Cerain, A. L. (2009). Ochratoxin Adecontamination: Areview. Foodcontrol, 20: 326-333. https://www.sciencedirect.com/science/article/abs/pii/S0956713508001631.
[11] Bueno, D., Muñoz, R. and Marty, J. L., 2014. Common methods to detect mycotoxins: a review with particular emphasis on electrochemical detection. Sensing in Electroanalysis. (K. Kalcher, R. Metelka, I. Švancara, K. Vytřas; Eds.). 2013/2014, Volume 8. https://dk.upce.cz/handle/10195/58390.
[12] Joint FAO/WHO Expert Committee on Food Additives. Meeting, 2001. Safety evaluation of certain mycotoxins in food (No. 74). Food & Agriculture Org. Control, 69: 65-73.
[13] IARC, International Agency for Research on Cancer. (1993). Some naturally occurring substance: Food items and constituents, heterocyclic aromatic amines, and Mycotoxins: IARC Monographs on the Evaluation of carcinogenic Risk of chemicals to Humans. pp. 571. https://www.cabdirect.org/cabdirect/abstract/19952006807.
[14] EC. (2006). Maximum limits for ochratoxin A. Regulation EC official Journal of Europian Union, 25: 3 https://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2006:364:0005:0024:EN:PDF.
[15] Raper, K. B. and Fennell, D. I. (1965). The genus Aspergillus. The genus Aspergillus.
[16] Klich, M. A. (2002). Identification of Common Aspergillus species. Netherlands Centraal bureau voor Schimmel culture.
[17] McClenny, N. (2005). Laboratory detection and identification of Aspergillus species by microscopic observation and culture: the traditional approach. Medical mycology, 43 (sup 1), pp. 125-128. https://doi.org/10.1080/13693780500052222.
[18] Pandit, P., Panta, O. P. and Karki, T. B., (2014). Isolation of Aspergillus ochraceus and Production of Ochratoxin in Coffee Samples. Nepal Journal of Science and Technology, 15: 133-138. https://www.nepjol.info/index.php/NJST/article/view/12030.
[19] Geremew, T., Abate, D., Landschoot, S., Haesaert, G., & Audenaert, K. (2016). Occurrence of toxigenic fungi and ochratoxinA in Ethiopian coffee for local consumption. Food Control, 69: 65-73. https://www.sciencedirect.com/science/article/abs/pii/S0956713516301943.
[20] Urbano, G. R., Taniwaki, M. H., Leitao, M. F. D. F. & Vicentini, M. C. (2001). Occurrence of ochratoxin A producingfungi in raw Brazilian coffee. Journal of Food Protection, 68, 1226–1230. https://meridian.allenpress.com/jfp/article/64/8/1226/170043/Occurrence-of-Ochratoxin-A-Producing-Fungi-in-Raw.
[21] CAST (Council for Agricultural Science Technology) (2003) Mycotoxins: Risks in plant, animal, human systems. Task Force Report, Ames, Iowa, No. 139, 1-199.
[22] Culliao, A. G. L.; Barcelo, J. M (2015). Fungal and mycotoxin contamination of coffee beans in Benguet province, Philippines. Food Addit. Contam. Part A 32, 250–260. https://www.tandfonline.com/doi/abs/10.1080/19440049.2014.1001796.
[23] Romani, S.; Sacchetti, G.; López, C. C.; Pinnavaia, G. G.; Dalla Rosa, M (2000). Screening on the occurrence of ochratoxinA in green coffee beans of different origins and types. J. Agric. Food Chem. 48: 3616–3619. https://pubs.acs.org/doi/abs/10.1021/jf990783b.
Cite This Article
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    Legese Hagos, Nagassa Dechassa, Kifle Belachew, Demelash Teferi, Solomon Yilma, et al. (2021). Isolation of Mycotoxigenic Fungi, Detection and Quantification of Ochratoxin A from Coffee (Coffea arabica L.). Journal of Drug Design and Medicinal Chemistry, 6(4), 47-52. https://doi.org/10.11648/j.jddmc.20200604.12

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    ACS Style

    Legese Hagos; Nagassa Dechassa; Kifle Belachew; Demelash Teferi; Solomon Yilma, et al. Isolation of Mycotoxigenic Fungi, Detection and Quantification of Ochratoxin A from Coffee (Coffea arabica L.). J. Drug Des. Med. Chem. 2021, 6(4), 47-52. doi: 10.11648/j.jddmc.20200604.12

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    AMA Style

    Legese Hagos, Nagassa Dechassa, Kifle Belachew, Demelash Teferi, Solomon Yilma, et al. Isolation of Mycotoxigenic Fungi, Detection and Quantification of Ochratoxin A from Coffee (Coffea arabica L.). J Drug Des Med Chem. 2021;6(4):47-52. doi: 10.11648/j.jddmc.20200604.12

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  • @article{10.11648/j.jddmc.20200604.12,
      author = {Legese Hagos and Nagassa Dechassa and Kifle Belachew and Demelash Teferi and Solomon Yilma and Gabisa Gidisa},
      title = {Isolation of Mycotoxigenic Fungi, Detection and Quantification of Ochratoxin A from Coffee (Coffea arabica L.)},
      journal = {Journal of Drug Design and Medicinal Chemistry},
      volume = {6},
      number = {4},
      pages = {47-52},
      doi = {10.11648/j.jddmc.20200604.12},
      url = {https://doi.org/10.11648/j.jddmc.20200604.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jddmc.20200604.12},
      abstract = {Introduction. Successful prevention of mother-to-child transmission (PMTCT) of the human immunodeficiency virus (HIV) requires early diagnosis, consistent access to antiretroviral therapy (ART), and regular health care during and after pregnancy. This study assessed the determinants of early HIV diagnosis by children born to HIV-positive (HIV+) mothers in the Kongo Central Province, Democratic Republic of the Congo (DRC). Methodology. Data from 230 HIV+ mothers screened under the PMTCT program between July 2015 and December 2017 were extracted from the databases of 31 Health Zones (HZ) of Kongo Central province. Data detailing laboratory and anthropometric findings, morbidity, and mortality were collected from each exposed child. The determinants of inaccessibility to early diagnosis were identified using logistic regression. Results. The mothers' mean age was 32.4 years, and 90.9% were on ART; 68.8% of children were older than 12 months at diagnosis. Diagnosis during the first 6 months of the child’s life was critical. Malnutrition was found in almost 90% of the children. Determinants of non-access to an early diagnosis were mothers who attained a primary education level and did not disclose their HIV status. Conclusion. All possible interventions should be considered to prevent mother-to-child HIV transmission. To ensure a child’s survival to their first birthday, early diagnosis should be performed soon after birth to an HIV+ mother and ART should be initiated.},
     year = {2021}
    }
    

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  • TY  - JOUR
    T1  - Isolation of Mycotoxigenic Fungi, Detection and Quantification of Ochratoxin A from Coffee (Coffea arabica L.)
    AU  - Legese Hagos
    AU  - Nagassa Dechassa
    AU  - Kifle Belachew
    AU  - Demelash Teferi
    AU  - Solomon Yilma
    AU  - Gabisa Gidisa
    Y1  - 2021/01/22
    PY  - 2021
    N1  - https://doi.org/10.11648/j.jddmc.20200604.12
    DO  - 10.11648/j.jddmc.20200604.12
    T2  - Journal of Drug Design and Medicinal Chemistry
    JF  - Journal of Drug Design and Medicinal Chemistry
    JO  - Journal of Drug Design and Medicinal Chemistry
    SP  - 47
    EP  - 52
    PB  - Science Publishing Group
    SN  - 2472-3576
    UR  - https://doi.org/10.11648/j.jddmc.20200604.12
    AB  - Introduction. Successful prevention of mother-to-child transmission (PMTCT) of the human immunodeficiency virus (HIV) requires early diagnosis, consistent access to antiretroviral therapy (ART), and regular health care during and after pregnancy. This study assessed the determinants of early HIV diagnosis by children born to HIV-positive (HIV+) mothers in the Kongo Central Province, Democratic Republic of the Congo (DRC). Methodology. Data from 230 HIV+ mothers screened under the PMTCT program between July 2015 and December 2017 were extracted from the databases of 31 Health Zones (HZ) of Kongo Central province. Data detailing laboratory and anthropometric findings, morbidity, and mortality were collected from each exposed child. The determinants of inaccessibility to early diagnosis were identified using logistic regression. Results. The mothers' mean age was 32.4 years, and 90.9% were on ART; 68.8% of children were older than 12 months at diagnosis. Diagnosis during the first 6 months of the child’s life was critical. Malnutrition was found in almost 90% of the children. Determinants of non-access to an early diagnosis were mothers who attained a primary education level and did not disclose their HIV status. Conclusion. All possible interventions should be considered to prevent mother-to-child HIV transmission. To ensure a child’s survival to their first birthday, early diagnosis should be performed soon after birth to an HIV+ mother and ART should be initiated.
    VL  - 6
    IS  - 4
    ER  - 

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Author Information
  • Ethiopian Institute of Agricultural Research, Jimma Agricultural Research Center, Jimma, Ethiopia

  • Ethiopian Institute of Agricultural Research, Ambo Plant Protection Research Center, Ambo, Ethiopia

  • Ethiopian Institute of Agricultural Research, Jimma Agricultural Research Center, Jimma, Ethiopia

  • Ethiopian Institute of Agricultural Research, Jimma Agricultural Research Center, Jimma, Ethiopia

  • Ethiopian Institute of Agricultural Research, Ambo Plant Protection Research Center, Ambo, Ethiopia

  • Ethiopian Institute of Agricultural Research, Jimma Agricultural Research Center, Jimma, Ethiopia

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