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Integrated Effect of Metal Accumulation, Oxidative Stress Responses and DNA Damage in Venerupis Decussata Gills Collected From Two Coast Tunisian Lagoons

Received: 12 October 2018     Accepted: 26 October 2018     Published: 16 November 2018
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Abstract

The bivalve Venerupis decussata has been proposed as a sentinel species for assessment of lagoon water. Our study aimed to evaluate the spatial and temporal variation of oxidative stress biomarkers, metal content and DNA damage in Venerupis decussata digestive gland collected seasonally from contaminated (BOUGHRARA “L2”) and comparatively cleaner ( GHAR EL MELH “L1”) lagoons. Trace metal contents (Cu, Pb and Cd) in Venerupis decussata collected at polluted site were 1-2 folds higher compared to the control site and showed maximum variation especially during summer and spring seasons. The current findings indicate a seasonal increase of malondialdehyde (MDA), protein carbonyl (PCO), glutathione (GSH) and metallothioneins (MT) levels in relation to trace element accumulation in Venerupis decussata digestive gland from BOUGHRARA sampling lagoon compared to those from GHAR EL MELH. We found an increase in glutathione-S-transfers (GST) and glutathione peroxides (GPx) activities in clams collected from BOUGHRARA lagoon. A random DNA degradation was observed mostly in digestive gland from the polluted site. The principal component examination of the physiological parameters showed a clear separation between Venerupis decussata collected from the polluted lagoon (L2) and those from the clean one (L1). Our study delivers basic information on the toxicological effects of environmental pollutants in clam through the combination of metabolic and physiological methodologies.

Published in Journal of Chemical, Environmental and Biological Engineering (Volume 2, Issue 2)
DOI 10.11648/j.jcebe.20180202.12
Page(s) 44-51
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Antioxidants Defense, Bioaccumulation, Calms, DNA Damage, Gills, Lagoons

References
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Cite This Article
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    Safa Bejaoui, Khaoula Telahigue, Imene Chetoui, Imen Rabeh, Chaima Fouzai, et al. (2018). Integrated Effect of Metal Accumulation, Oxidative Stress Responses and DNA Damage in Venerupis Decussata Gills Collected From Two Coast Tunisian Lagoons. Journal of Chemical, Environmental and Biological Engineering, 2(2), 44-51. https://doi.org/10.11648/j.jcebe.20180202.12

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    ACS Style

    Safa Bejaoui; Khaoula Telahigue; Imene Chetoui; Imen Rabeh; Chaima Fouzai, et al. Integrated Effect of Metal Accumulation, Oxidative Stress Responses and DNA Damage in Venerupis Decussata Gills Collected From Two Coast Tunisian Lagoons. J. Chem. Environ. Biol. Eng. 2018, 2(2), 44-51. doi: 10.11648/j.jcebe.20180202.12

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    AMA Style

    Safa Bejaoui, Khaoula Telahigue, Imene Chetoui, Imen Rabeh, Chaima Fouzai, et al. Integrated Effect of Metal Accumulation, Oxidative Stress Responses and DNA Damage in Venerupis Decussata Gills Collected From Two Coast Tunisian Lagoons. J Chem Environ Biol Eng. 2018;2(2):44-51. doi: 10.11648/j.jcebe.20180202.12

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  • @article{10.11648/j.jcebe.20180202.12,
      author = {Safa Bejaoui and Khaoula Telahigue and Imene Chetoui and Imen Rabeh and Chaima Fouzai and Wafa Trabelsi and Ines Houas-Gharsallah and M’hamed El Cafsi and Nejla Soudani},
      title = {Integrated Effect of Metal Accumulation, Oxidative Stress Responses and DNA Damage in Venerupis Decussata Gills Collected From Two Coast Tunisian Lagoons},
      journal = {Journal of Chemical, Environmental and Biological Engineering},
      volume = {2},
      number = {2},
      pages = {44-51},
      doi = {10.11648/j.jcebe.20180202.12},
      url = {https://doi.org/10.11648/j.jcebe.20180202.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jcebe.20180202.12},
      abstract = {The bivalve Venerupis decussata has been proposed as a sentinel species for assessment of lagoon water. Our study aimed to evaluate the spatial and temporal variation of oxidative stress biomarkers, metal content and DNA damage in Venerupis decussata digestive gland collected seasonally from contaminated (BOUGHRARA “L2”) and comparatively cleaner ( GHAR EL MELH “L1”) lagoons. Trace metal contents (Cu, Pb and Cd) in Venerupis decussata collected at polluted site were 1-2 folds higher compared to the control site and showed maximum variation especially during summer and spring seasons. The current findings indicate a seasonal increase of malondialdehyde (MDA), protein carbonyl (PCO), glutathione (GSH) and metallothioneins (MT) levels in relation to trace element accumulation in Venerupis decussata digestive gland from BOUGHRARA sampling lagoon compared to those from GHAR EL MELH. We found an increase in glutathione-S-transfers (GST) and glutathione peroxides (GPx) activities in clams collected from BOUGHRARA lagoon. A random DNA degradation was observed mostly in digestive gland from the polluted site. The principal component examination of the physiological parameters showed a clear separation between Venerupis decussata collected from the polluted lagoon (L2) and those from the clean one (L1). Our study delivers basic information on the toxicological effects of environmental pollutants in clam through the combination of metabolic and physiological methodologies.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Integrated Effect of Metal Accumulation, Oxidative Stress Responses and DNA Damage in Venerupis Decussata Gills Collected From Two Coast Tunisian Lagoons
    AU  - Safa Bejaoui
    AU  - Khaoula Telahigue
    AU  - Imene Chetoui
    AU  - Imen Rabeh
    AU  - Chaima Fouzai
    AU  - Wafa Trabelsi
    AU  - Ines Houas-Gharsallah
    AU  - M’hamed El Cafsi
    AU  - Nejla Soudani
    Y1  - 2018/11/16
    PY  - 2018
    N1  - https://doi.org/10.11648/j.jcebe.20180202.12
    DO  - 10.11648/j.jcebe.20180202.12
    T2  - Journal of Chemical, Environmental and Biological Engineering
    JF  - Journal of Chemical, Environmental and Biological Engineering
    JO  - Journal of Chemical, Environmental and Biological Engineering
    SP  - 44
    EP  - 51
    PB  - Science Publishing Group
    SN  - 2640-267X
    UR  - https://doi.org/10.11648/j.jcebe.20180202.12
    AB  - The bivalve Venerupis decussata has been proposed as a sentinel species for assessment of lagoon water. Our study aimed to evaluate the spatial and temporal variation of oxidative stress biomarkers, metal content and DNA damage in Venerupis decussata digestive gland collected seasonally from contaminated (BOUGHRARA “L2”) and comparatively cleaner ( GHAR EL MELH “L1”) lagoons. Trace metal contents (Cu, Pb and Cd) in Venerupis decussata collected at polluted site were 1-2 folds higher compared to the control site and showed maximum variation especially during summer and spring seasons. The current findings indicate a seasonal increase of malondialdehyde (MDA), protein carbonyl (PCO), glutathione (GSH) and metallothioneins (MT) levels in relation to trace element accumulation in Venerupis decussata digestive gland from BOUGHRARA sampling lagoon compared to those from GHAR EL MELH. We found an increase in glutathione-S-transfers (GST) and glutathione peroxides (GPx) activities in clams collected from BOUGHRARA lagoon. A random DNA degradation was observed mostly in digestive gland from the polluted site. The principal component examination of the physiological parameters showed a clear separation between Venerupis decussata collected from the polluted lagoon (L2) and those from the clean one (L1). Our study delivers basic information on the toxicological effects of environmental pollutants in clam through the combination of metabolic and physiological methodologies.
    VL  - 2
    IS  - 2
    ER  - 

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Author Information
  • Department of Biology, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia

  • Department of Biology, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia

  • Department of Biology, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia

  • Department of Biology, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia

  • Department of Biology, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia

  • Department of Biology, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia

  • Higher Institute of Marine Sciences and Technology, La Goulette Center, Tunis, Tunisia

  • Department of Biology, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia

  • Department of Biology, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia

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