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Cornea Confocal Microscopy Study of Patients with Preclinical Keratoconus

Received: 15 February 2022     Accepted: 4 March 2022     Published: 12 March 2022
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Abstract

Purpose: To report the difference of corneal confocal microscopy examination in patients with preclinical keratoconus or clinical keratoconus. Methods: 8 unilateral keratoconus patients were examined by confocal microscopy before they had cross-linking surgery. As control groups, 23 patients with myopia and 13 patients with keratoconus in both eyes were also examined by confocal microscopy. Then we used Image J to compared the density of epithelium cell, length of cornea nerve fiber, density of Langerhans cell, density of keratocyte in shallow, middle, deep stroma, and the width and grayscale value of fold near Descement membrane for each group. Result: There was differences in corneal epithelial cell count between three groups (4358.27 ± 635.14 cells/mm2 versus 4057.81 ± 316.29 cells/mm2 versus 3522.65 ± 978.10 cells/mm2). The density of keratocyte in stroma showed a tendency to decrease with increasing depth. The density of keratocyte in keratoconus was less than that in normal eyes. The fold showed much wider and daker in keratoconus eyes than in normal eyes. The density of Langerhans cell was more in keratoconus group than it in normal group. Conclusion: We first report the differences in corneal epithelial cell count and fold near Descement membrane. It might provide a new way to diagnose early keratoconus. The difference of density of Langerhans cell suggested us the possibility of inflammation in keratoconus.

Published in International Journal of Ophthalmology & Visual Science (Volume 7, Issue 1)
DOI 10.11648/j.ijovs.20220701.17
Page(s) 40-44
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Confocal, Keratoconus, Epithelial Cell, Fold

References
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[2] Weed KH, MacEwen CJ, Cox A, McGhee CN: Quantitative analysis of corneal microstructure in keratoconus utilising in vivo confocal microscopy. Eye (Lond) 2007, 21 (5): 614-623.
[3] Mocan MC, Yilmaz PT, Irkec M, Orhan M: In vivo confocal microscopy for the evaluation of corneal microstructure in keratoconus. Curr Eye Res 2008, 33 (11): 933-939.
[4] Alvani A, Hashemi H, Pakravan M, Mahbod M, Amanzadeh K, Seyedian MA, Yaseri M, Jafarzadehpur E, Fotouhi A: Dynamic corneal biomechanics in different cell layers: in keratoconus and normal eyes. Ophthalmic Physiol Opt 2021, 41 (2): 414-423.
[5] Bitirgen G, Ozkagnici A, Malik RA, Oltulu R: Evaluation of contact lens-induced changes in keratoconic corneas using in vivo confocal microscopy. Invest Ophthalmol Vis Sci 2013, 54 (8): 5385-5391.
[6] Bitirgen G, Ozkagnici A, Bozkurt B, Malik RA: In vivo corneal confocal microscopic analysis in patients with keratoconus. Int J Ophthalmol 2015, 8 (3): 534-539.
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Cite This Article
  • APA Style

    Jing Yang, Minhui Wu, Yi Wu, Runzhang He, Yating Nong, et al. (2022). Cornea Confocal Microscopy Study of Patients with Preclinical Keratoconus. International Journal of Ophthalmology & Visual Science, 7(1), 40-44. https://doi.org/10.11648/j.ijovs.20220701.17

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    ACS Style

    Jing Yang; Minhui Wu; Yi Wu; Runzhang He; Yating Nong, et al. Cornea Confocal Microscopy Study of Patients with Preclinical Keratoconus. Int. J. Ophthalmol. Vis. Sci. 2022, 7(1), 40-44. doi: 10.11648/j.ijovs.20220701.17

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    AMA Style

    Jing Yang, Minhui Wu, Yi Wu, Runzhang He, Yating Nong, et al. Cornea Confocal Microscopy Study of Patients with Preclinical Keratoconus. Int J Ophthalmol Vis Sci. 2022;7(1):40-44. doi: 10.11648/j.ijovs.20220701.17

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  • @article{10.11648/j.ijovs.20220701.17,
      author = {Jing Yang and Minhui Wu and Yi Wu and Runzhang He and Yating Nong and Chun Zhang and Sheng Zhou},
      title = {Cornea Confocal Microscopy Study of Patients with Preclinical Keratoconus},
      journal = {International Journal of Ophthalmology & Visual Science},
      volume = {7},
      number = {1},
      pages = {40-44},
      doi = {10.11648/j.ijovs.20220701.17},
      url = {https://doi.org/10.11648/j.ijovs.20220701.17},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijovs.20220701.17},
      abstract = {Purpose: To report the difference of corneal confocal microscopy examination in patients with preclinical keratoconus or clinical keratoconus. Methods: 8 unilateral keratoconus patients were examined by confocal microscopy before they had cross-linking surgery. As control groups, 23 patients with myopia and 13 patients with keratoconus in both eyes were also examined by confocal microscopy. Then we used Image J to compared the density of epithelium cell, length of cornea nerve fiber, density of Langerhans cell, density of keratocyte in shallow, middle, deep stroma, and the width and grayscale value of fold near Descement membrane for each group. Result: There was differences in corneal epithelial cell count between three groups (4358.27 ± 635.14 cells/mm2 versus 4057.81 ± 316.29 cells/mm2 versus 3522.65 ± 978.10 cells/mm2). The density of keratocyte in stroma showed a tendency to decrease with increasing depth. The density of keratocyte in keratoconus was less than that in normal eyes. The fold showed much wider and daker in keratoconus eyes than in normal eyes. The density of Langerhans cell was more in keratoconus group than it in normal group. Conclusion: We first report the differences in corneal epithelial cell count and fold near Descement membrane. It might provide a new way to diagnose early keratoconus. The difference of density of Langerhans cell suggested us the possibility of inflammation in keratoconus.},
     year = {2022}
    }
    

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  • TY  - JOUR
    T1  - Cornea Confocal Microscopy Study of Patients with Preclinical Keratoconus
    AU  - Jing Yang
    AU  - Minhui Wu
    AU  - Yi Wu
    AU  - Runzhang He
    AU  - Yating Nong
    AU  - Chun Zhang
    AU  - Sheng Zhou
    Y1  - 2022/03/12
    PY  - 2022
    N1  - https://doi.org/10.11648/j.ijovs.20220701.17
    DO  - 10.11648/j.ijovs.20220701.17
    T2  - International Journal of Ophthalmology & Visual Science
    JF  - International Journal of Ophthalmology & Visual Science
    JO  - International Journal of Ophthalmology & Visual Science
    SP  - 40
    EP  - 44
    PB  - Science Publishing Group
    SN  - 2637-3858
    UR  - https://doi.org/10.11648/j.ijovs.20220701.17
    AB  - Purpose: To report the difference of corneal confocal microscopy examination in patients with preclinical keratoconus or clinical keratoconus. Methods: 8 unilateral keratoconus patients were examined by confocal microscopy before they had cross-linking surgery. As control groups, 23 patients with myopia and 13 patients with keratoconus in both eyes were also examined by confocal microscopy. Then we used Image J to compared the density of epithelium cell, length of cornea nerve fiber, density of Langerhans cell, density of keratocyte in shallow, middle, deep stroma, and the width and grayscale value of fold near Descement membrane for each group. Result: There was differences in corneal epithelial cell count between three groups (4358.27 ± 635.14 cells/mm2 versus 4057.81 ± 316.29 cells/mm2 versus 3522.65 ± 978.10 cells/mm2). The density of keratocyte in stroma showed a tendency to decrease with increasing depth. The density of keratocyte in keratoconus was less than that in normal eyes. The fold showed much wider and daker in keratoconus eyes than in normal eyes. The density of Langerhans cell was more in keratoconus group than it in normal group. Conclusion: We first report the differences in corneal epithelial cell count and fold near Descement membrane. It might provide a new way to diagnose early keratoconus. The difference of density of Langerhans cell suggested us the possibility of inflammation in keratoconus.
    VL  - 7
    IS  - 1
    ER  - 

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Author Information
  • State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China

  • State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China

  • State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China

  • State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China

  • State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China

  • State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China

  • State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China

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