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The Potential Roles of CDO1 and JAM3 Methylation in the Occurrence of Nasopharyngeal Carcinoma

Received: 22 June 2023     Accepted: 10 July 2023     Published: 20 July 2023
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Abstract

Background: Nasopharyngeal carcinoma (NPC) is a highly malignant tumor derived from nasopharyngeal epithelial tissue. Due to their strong capacity for hidden lesions and unobvious early symptoms, Given the critical problem for clinical diagnosis, NPC has a high rate of misdiagnosed and missed, which seriously threatens human health. The underlying mechanism of the occurrence and development of nasopharyngeal carcinoma is still not elucidated. In recent years, increasing attention has been paid to the study of some tumor-related genes, especially the epigenetic regulation of genes. Objective: To investigate the role of CDO1 and JAM3 gene methylation in the occurrence of nasopharyngeal carcinoma. Methods: Methylation-specific PCR and pyrosequencing were used to detect the difference in methylation rates of CDO1 and JAM3 in nasopharyngeal carcinoma and nasopharyngeal chronic inflammation tissues. The abundance of protein expression and mRNA for CDO1 and JAM3 were detected by fluorescence quantitative PCR and Western Blot in nasopharyngeal carcinoma and chronic nasopharyngitis tissues. Results: Pyrosequencing indicated that the methylation rates of CDO1 and JAM3 in nasopharyngeal carcinoma tissues were remarkably increased, compared with nasopharyngeal chronic inflammation tissues. Furthermore, the qPCR and Western Blot suggested that the expression of CDO1 and JAM3 in nasopharyngeal carcinoma tissues was significantly lower than that in nasopharyngeal chronic inflammation tissues. Conclusion: The methylation of the CpG island in the promoter region of CDO1 and JAM3 genes leads to a decrease in their gene expression, which may be related to the occurrence of nasopharyngeal carcinoma.

Published in International Journal of Otorhinolaryngology (Volume 9, Issue 2)
DOI 10.11648/j.ijo.20230902.12
Page(s) 20-25
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

Nasopharyngeal Carcinoma, Cdo1, Jam3, Methylation

References
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[2] Yoshizaki, T., et al., Pathogenic role of Epstein-Barr virus latent membrane protein-1 in the development of nasopharyngeal carcinoma. Cancer Lett, 2013, 37 (1): p. 1-7.
[3] Xu FH, Xiong D, Xu YF, et al. An epidemiological and molecular study of the relationship between smoking, risk of nasopharyngeal carcinoma, and Epstein-Barr virus activation [J]. J Natl Cancer Inst, 2012, 104 (18): 1396-1410.
[4] Brait M, Ling S, Nagpal J K, Chang X et al. Cysteine Dioxygenase1 Is a Tumor Suppressor Gene Silenced by Promoter Methylation in Multiple Human Cancers. PLOS ONE (2012), 7 (9), e44951.
[5] Zhou D, Tang W, Zhang Y, An HX. JAM3 functions as a novel tumor suppressor and is inactivated by DNA methylation in colorectal cancer. Cancer Management and Research 2019: 11 2457–2470.
[6] Kojima K, Nakamura T, Ohbu M, Katoh H, Ooizumi Y, Igarashi K, Ishii S. et al. Cysteine dioxygenase type 1 (CDO1) gene promoter methylation during the adenomacarcinoma sequence in colorectal cancer [J]. PLoS ONE. 2018; 13 (5): e0194785.
[7] Igarashi K, Yamashita K, Katoh H, Kojima K, Ooizumi Y, Nishizawa N, et al. Prognostic significance of promoter DNA hypermethylation of the cysteine dioxygenase 1 (CDO1) gene in primary gallbladder cancer and gallbladder disease. PLoS One. 2017; 12 (11): e0188178.
[8] Ushiku H, Yamashita K, Katoh H, Ema A, Minatani N, Kikuchi M, et al. Promoter DNA methylation of CDO1 gene and its clinical significance in esophageal squamous cell carcinoma. Dis Esophagus. 2017; 30 (2): 1–9.
[9] Yang Y, Fan YC, Gao S, Dou CY, et al. methylated cysteine dioxygenase-1 gene promoter in the serum is a potential biomarker for hepatitis B virus-related hepatocellular carcinoma. Tohoku J Exp Med, 2014; 232 (3): 187-94.
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[11] Chen CP, Wang LK, Chen CY, et al. Decreased junctional adhesion molecule 3 expression induces reactive oxygen species production and apoptosis in trophoblasts. Biol Reprod. 2022 Nov 14; 107 (5): 1264-1278.
[12] Kong WJ, Zhang S, Guo C, Zhang S, Wang Y, Zhang D, et al. Methylation- associated silencing of death- protein kinase gene in laryngeal squamous cell cancer. Laryngoscope 2005; 115: 1395-1401.
[13] Kong WJ, Zhang S, Guo CK, Wang YJ, Chen X, Zhang SL, Zhang D, Liu Z and Kong W. Effect of methylation-associated silencing of the deathassociated protein kinase gene on nasopharyngeal carcinoma. Anti-Cancer Drugs, 2006, 17 (3): 251-259.
[14] Zhang S, Li S and Gao JL. Promoter methylation status of the tumor suppressor gene SOX11 is associated with cell growth and invasion in nasopharyngeal carcinoma. Cancer Cell International, 2013, 13: 109.
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  • APA Style

    Zhang, S., Sai, C., Lin, Y., Tang, J., Yuan, H. (2023). The Potential Roles of CDO1 and JAM3 Methylation in the Occurrence of Nasopharyngeal Carcinoma. International Journal of Otorhinolaryngology, 9(2), 20-25. https://doi.org/10.11648/j.ijo.20230902.12

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    ACS Style

    Zhang, S.; Sai, C.; Lin, Y.; Tang, J.; Yuan, H. The Potential Roles of CDO1 and JAM3 Methylation in the Occurrence of Nasopharyngeal Carcinoma. Int. J. Otorhinolaryngol. 2023, 9(2), 20-25. doi: 10.11648/j.ijo.20230902.12

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    AMA Style

    Zhang S, Sai C, Lin Y, Tang J, Yuan H. The Potential Roles of CDO1 and JAM3 Methylation in the Occurrence of Nasopharyngeal Carcinoma. Int J Otorhinolaryngol. 2023;9(2):20-25. doi: 10.11648/j.ijo.20230902.12

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  • @article{10.11648/j.ijo.20230902.12,
      author = {Song Zhang and Chong Sai and Yi-Ling Lin and Jia-Hui Tang and Hong-Yan Yuan},
      title = {The Potential Roles of CDO1 and JAM3 Methylation in the Occurrence of Nasopharyngeal Carcinoma},
      journal = {International Journal of Otorhinolaryngology},
      volume = {9},
      number = {2},
      pages = {20-25},
      doi = {10.11648/j.ijo.20230902.12},
      url = {https://doi.org/10.11648/j.ijo.20230902.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijo.20230902.12},
      abstract = {Background: Nasopharyngeal carcinoma (NPC) is a highly malignant tumor derived from nasopharyngeal epithelial tissue. Due to their strong capacity for hidden lesions and unobvious early symptoms, Given the critical problem for clinical diagnosis, NPC has a high rate of misdiagnosed and missed, which seriously threatens human health. The underlying mechanism of the occurrence and development of nasopharyngeal carcinoma is still not elucidated. In recent years, increasing attention has been paid to the study of some tumor-related genes, especially the epigenetic regulation of genes. Objective: To investigate the role of CDO1 and JAM3 gene methylation in the occurrence of nasopharyngeal carcinoma. Methods: Methylation-specific PCR and pyrosequencing were used to detect the difference in methylation rates of CDO1 and JAM3 in nasopharyngeal carcinoma and nasopharyngeal chronic inflammation tissues. The abundance of protein expression and mRNA for CDO1 and JAM3 were detected by fluorescence quantitative PCR and Western Blot in nasopharyngeal carcinoma and chronic nasopharyngitis tissues. Results: Pyrosequencing indicated that the methylation rates of CDO1 and JAM3 in nasopharyngeal carcinoma tissues were remarkably increased, compared with nasopharyngeal chronic inflammation tissues. Furthermore, the qPCR and Western Blot suggested that the expression of CDO1 and JAM3 in nasopharyngeal carcinoma tissues was significantly lower than that in nasopharyngeal chronic inflammation tissues. Conclusion: The methylation of the CpG island in the promoter region of CDO1 and JAM3 genes leads to a decrease in their gene expression, which may be related to the occurrence of nasopharyngeal carcinoma.},
     year = {2023}
    }
    

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  • TY  - JOUR
    T1  - The Potential Roles of CDO1 and JAM3 Methylation in the Occurrence of Nasopharyngeal Carcinoma
    AU  - Song Zhang
    AU  - Chong Sai
    AU  - Yi-Ling Lin
    AU  - Jia-Hui Tang
    AU  - Hong-Yan Yuan
    Y1  - 2023/07/20
    PY  - 2023
    N1  - https://doi.org/10.11648/j.ijo.20230902.12
    DO  - 10.11648/j.ijo.20230902.12
    T2  - International Journal of Otorhinolaryngology
    JF  - International Journal of Otorhinolaryngology
    JO  - International Journal of Otorhinolaryngology
    SP  - 20
    EP  - 25
    PB  - Science Publishing Group
    SN  - 2472-2413
    UR  - https://doi.org/10.11648/j.ijo.20230902.12
    AB  - Background: Nasopharyngeal carcinoma (NPC) is a highly malignant tumor derived from nasopharyngeal epithelial tissue. Due to their strong capacity for hidden lesions and unobvious early symptoms, Given the critical problem for clinical diagnosis, NPC has a high rate of misdiagnosed and missed, which seriously threatens human health. The underlying mechanism of the occurrence and development of nasopharyngeal carcinoma is still not elucidated. In recent years, increasing attention has been paid to the study of some tumor-related genes, especially the epigenetic regulation of genes. Objective: To investigate the role of CDO1 and JAM3 gene methylation in the occurrence of nasopharyngeal carcinoma. Methods: Methylation-specific PCR and pyrosequencing were used to detect the difference in methylation rates of CDO1 and JAM3 in nasopharyngeal carcinoma and nasopharyngeal chronic inflammation tissues. The abundance of protein expression and mRNA for CDO1 and JAM3 were detected by fluorescence quantitative PCR and Western Blot in nasopharyngeal carcinoma and chronic nasopharyngitis tissues. Results: Pyrosequencing indicated that the methylation rates of CDO1 and JAM3 in nasopharyngeal carcinoma tissues were remarkably increased, compared with nasopharyngeal chronic inflammation tissues. Furthermore, the qPCR and Western Blot suggested that the expression of CDO1 and JAM3 in nasopharyngeal carcinoma tissues was significantly lower than that in nasopharyngeal chronic inflammation tissues. Conclusion: The methylation of the CpG island in the promoter region of CDO1 and JAM3 genes leads to a decrease in their gene expression, which may be related to the occurrence of nasopharyngeal carcinoma.
    VL  - 9
    IS  - 2
    ER  - 

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Author Information
  • Department of Otolaryngology, University of Chinese Academy of Science-Shenzhen Hospital, Shenzhen, China

  • Department of Otolaryngology, University of Chinese Academy of Science-Shenzhen Hospital, Shenzhen, China

  • Department of Otolaryngology, University of Chinese Academy of Science-Shenzhen Hospital, Shenzhen, China

  • Department of Otolaryngology, University of Chinese Academy of Science-Shenzhen Hospital, Shenzhen, China

  • Department of Otolaryngology, University of Chinese Academy of Science-Shenzhen Hospital, Shenzhen, China

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