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Transcriptome Changes Associated with Protective Immunity in T and B Cell-Deficient Rag1-/- Mutant Zebrafish

Received: 22 January 2017     Accepted: 21 February 2017     Published: 25 March 2017
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Abstract

To elucidate the basis of protective immunity in T and B cell deficient rag1-/- mutant zebrafish, we conducted microarray analysis of 15,617 genes from rag1-/- mutant zebrafish 48 hours after a primary response and 48 hours after a secondary response. Following primary exposure, the highest fold expression differences (3.8 to 4.95) were genes for serum amyloid A, chemokine CCL-C5a (CCL-19a), signal transducer and activator of transcription (STAT) 1b, interferon regulatory factor 11, and myxovirus resistance A. Strong induction of these genes demonstrated that primary immune responses and innate immune cells were not impaired in T and B cell deficient mutant zebrafish. Following bacterial re-exposure, the highest fold expression differences (2 to 3 fold) were in chemokine CCL-C5a (CCL-19a), myomegalin, bone morphogenetic protein 4, and relaxin 3a. These genes are involved in the immune response and cell proliferation. Genes for cell receptor activation and signal transduction, cell proliferation and cytotoxic functions were also up-regulated. These findings suggest receptor activation and expansion of a cell population. Increased ifnγ expression at 48 hpi was associated with both primary and secondary immune responses.

Published in International Journal of Immunology (Volume 5, Issue 2)
DOI 10.11648/j.iji.20170502.11
Page(s) 20-36
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

rag1-/- Mutant Zebrafish, Edwardsiella ictaluri, Protective Immunity, Transcriptome, Cell-Mediated Immunity

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    Aparna Krishnavajhala, Preeti J. Muire, Larry Hanson, Henry Wan, Fiona McCarthy, et al. (2017). Transcriptome Changes Associated with Protective Immunity in T and B Cell-Deficient Rag1-/- Mutant Zebrafish. International Journal of Immunology, 5(2), 20-36. https://doi.org/10.11648/j.iji.20170502.11

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    Aparna Krishnavajhala; Preeti J. Muire; Larry Hanson; Henry Wan; Fiona McCarthy, et al. Transcriptome Changes Associated with Protective Immunity in T and B Cell-Deficient Rag1-/- Mutant Zebrafish. Int. J. Immunol. 2017, 5(2), 20-36. doi: 10.11648/j.iji.20170502.11

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    AMA Style

    Aparna Krishnavajhala, Preeti J. Muire, Larry Hanson, Henry Wan, Fiona McCarthy, et al. Transcriptome Changes Associated with Protective Immunity in T and B Cell-Deficient Rag1-/- Mutant Zebrafish. Int J Immunol. 2017;5(2):20-36. doi: 10.11648/j.iji.20170502.11

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  • @article{10.11648/j.iji.20170502.11,
      author = {Aparna Krishnavajhala and Preeti J. Muire and Larry Hanson and Henry Wan and Fiona McCarthy and Alan Zhou and Lora Petrie-Hanson},
      title = {Transcriptome Changes Associated with Protective Immunity in T and B Cell-Deficient Rag1-/-  Mutant Zebrafish},
      journal = {International Journal of Immunology},
      volume = {5},
      number = {2},
      pages = {20-36},
      doi = {10.11648/j.iji.20170502.11},
      url = {https://doi.org/10.11648/j.iji.20170502.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.iji.20170502.11},
      abstract = {To elucidate the basis of protective immunity in T and B cell deficient rag1-/- mutant zebrafish, we conducted microarray analysis of 15,617 genes from rag1-/- mutant zebrafish 48 hours after a primary response and 48 hours after a secondary response. Following primary exposure, the highest fold expression differences (3.8 to 4.95) were genes for serum amyloid A, chemokine CCL-C5a (CCL-19a), signal transducer and activator of transcription (STAT) 1b, interferon regulatory factor 11, and myxovirus resistance A. Strong induction of these genes demonstrated that primary immune responses and innate immune cells were not impaired in T and B cell deficient mutant zebrafish. Following bacterial re-exposure, the highest fold expression differences (2 to 3 fold) were in chemokine CCL-C5a (CCL-19a), myomegalin, bone morphogenetic protein 4, and relaxin 3a. These genes are involved in the immune response and cell proliferation. Genes for cell receptor activation and signal transduction, cell proliferation and cytotoxic functions were also up-regulated. These findings suggest receptor activation and expansion of a cell population. Increased ifnγ expression at 48 hpi was associated with both primary and secondary immune responses.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Transcriptome Changes Associated with Protective Immunity in T and B Cell-Deficient Rag1-/-  Mutant Zebrafish
    AU  - Aparna Krishnavajhala
    AU  - Preeti J. Muire
    AU  - Larry Hanson
    AU  - Henry Wan
    AU  - Fiona McCarthy
    AU  - Alan Zhou
    AU  - Lora Petrie-Hanson
    Y1  - 2017/03/25
    PY  - 2017
    N1  - https://doi.org/10.11648/j.iji.20170502.11
    DO  - 10.11648/j.iji.20170502.11
    T2  - International Journal of Immunology
    JF  - International Journal of Immunology
    JO  - International Journal of Immunology
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    EP  - 36
    PB  - Science Publishing Group
    SN  - 2329-1753
    UR  - https://doi.org/10.11648/j.iji.20170502.11
    AB  - To elucidate the basis of protective immunity in T and B cell deficient rag1-/- mutant zebrafish, we conducted microarray analysis of 15,617 genes from rag1-/- mutant zebrafish 48 hours after a primary response and 48 hours after a secondary response. Following primary exposure, the highest fold expression differences (3.8 to 4.95) were genes for serum amyloid A, chemokine CCL-C5a (CCL-19a), signal transducer and activator of transcription (STAT) 1b, interferon regulatory factor 11, and myxovirus resistance A. Strong induction of these genes demonstrated that primary immune responses and innate immune cells were not impaired in T and B cell deficient mutant zebrafish. Following bacterial re-exposure, the highest fold expression differences (2 to 3 fold) were in chemokine CCL-C5a (CCL-19a), myomegalin, bone morphogenetic protein 4, and relaxin 3a. These genes are involved in the immune response and cell proliferation. Genes for cell receptor activation and signal transduction, cell proliferation and cytotoxic functions were also up-regulated. These findings suggest receptor activation and expansion of a cell population. Increased ifnγ expression at 48 hpi was associated with both primary and secondary immune responses.
    VL  - 5
    IS  - 2
    ER  - 

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  • Department of Pediatrics, National School of Tropical Medicine, Baylor College of Medicine, Houston, Texas, USA

  • Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, USA

  • Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, USA

  • Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, USA

  • School of Animal and Comparative Biomedical Sciences, University of Arizona, Tucson, USA

  • Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, USA

  • Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, USA

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