International Journal of Genetics and Genomics

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Identification and Genetic Characterisation of Populations of Tuta absoluta (Meyrick, 1917; Lepidoptera: Gelechidae) Destroyer of Tomato Farming in Senegal

Received: Aug. 18, 2023    Accepted: Sep. 04, 2023    Published: Sep. 14, 2023
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Abstract

In Senegal tomato jeopardiser Tuta absoluta (Meyrick 1917; Lepidoptera: Gelechiidae) causes serious losses in tomato production. This species can feed on all the green parts of the tomato and can cause up to 100% damage if not controlled. Currently, the most effective method of pest management is the use of chemicals. However, as it is generally noticed, excessive and unconscious use of chemicals leads to very harmful environmental pollution. The objective of this study is to identify the haplotypes of Tuta absoluta but also to see if agro-ecological zones and localities have contributed to genetically structure its populations. To achieve this goal, we adopted the PCR-Sequencing technique of the mitochondrial gene Cytochrome B of three populations of T. absoluta from three different localities in Senegal. The study has revealed the presence of 8 haplotypes and a population of Tuta absoluta in demographic balance. No significant genetic structuring has been noted between agro-ecological zones or localities. Moreover, phylogenetic reconstructions show no clustering either according to agro-ecological zones (Niayes and the Senegal River Valley), or according to localities. Nevertheless two clades were revealed by the latter, suggesting a multiple introduction of T. absoluta followed by an expansion to different geographical regions of the country by diffusion after its introduction.

DOI 10.11648/j.ijgg.20231103.17
Published in International Journal of Genetics and Genomics ( Volume 11, Issue 3, September 2023 )
Page(s) 104-111
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Agro Ecological Zones, Genetic Structuring, Localities, Haplotype, Jeopardiser

References
[1] Abdul-Rassoul MS. A. (2014). New Host Record for Tomato Leaf Miner Tuta Absoluta (Meyrick, 1917) in Baghdad Province, Iraq. Bull. Iraq Nat. Hist. Mus. 13(1): 15-18.
[2] Aroun M. E., Bentafat A. & Djazouli Z. (2014) Effet comparé phytosanitaire d'un jus de lombricompost Fermenté sur poudre de vesce avoine et d'une huile Essentielle du thym sur une culture Sous serre de tomate v. Marmande, Revue Agrobiologia 6: 60-66.
[3] Asma C., Glaucia M., Wiem H., Sabrine B. A., Axel H. & Kaouthar L. G. (2017). Some remarks on the genetic uniformity of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae). J. Entomol Zool Stud. 5: 1380-1382.
[4] Avise J. C. (2000). Phylogeography: the history and formation of species. Harvard University Press, Cambridge, MA.
[5] Bandelt H. J., Forster P. & Röhl A. (1999). Median-joining networks for inferring intraspecific phylogenies. Mol. Biol. Evol. 16: 37–48.
[6] Barry D., Kafom A. C, Diome T., Ndiaye N. P, Thiaw C., Ndiaye S. & Sembène M. (2017). Impact of Agro-Ecological Zones on Genetic Structuration of Callosobruchus maculatus F (Coleoptera: Bruchidae) the Major Pest Weevil in West Africa. International Journal of Science and Research Ecologique, 8(2): 121-139.
[7] Berkani A. & Badaoui M. I. (2008). La mineuse de la tomate Tuta absoluta Meyrick 1917 (Lepidoptera: Gelechiidae). Annales de l’Institut National de la Recherche Agronomique d'Algérie 16 p.
[8] Bettaibi A., Mezghani K. M., Bouktila D., Makni H. & Makni M. (2012). Genetic variability of the tomato leaf miner (Tuta absoluta Meyrick; Lepidoptera: Gelechiidae), in Tunisia, inferred from RAPD-PCR, Chilean J. Agric. Res. 2: 212–216.
[9] Chougar S. (2011). Bioécologie de la mineuse de la Tomate Tuta absoluta (Meyrick, 1917). Thèse de Doctorat, Universite Mouloud Mammeri de Tizi-ouzou 169p.
[10] Carvalho G. A, Reis P. R, Rocha L. C. D, Moraes J. C., Fuini L. C. & Ecole CC. (2003). Side effects of insecticides used in tomato fields on Trichogramma pretiosum (Hy menoptera: Trichogrammatida£). Acta Scientiarum. Agronomy. Maringá, 25(2): 275-279.
[11] Cifuentes D, Chynoweth R, Bielza P. (2011). Genetic study of Mediterranean and South American populations of tomato leaf miner Tuta absoluta (Povolny, 1994) (Lepidoptera: Gelechiidae) using ribosomal and mitochondrial markers. Pest Manag Sci. 67: 1155-1162.
[12] Dia C. A. K. M., Sarr A. G. R. J., Kafom A., Ngom D., Diome T., Thiaw C., Ndiaye S. & Sembène M. (2018). Morphological identification of trophic Tribolium castaneum populations herbst (Coleoptera, Tenebrionidae) in West Africa. International Journal of Advanced Research 6(1): 203-216.
[13] Diome T., Thiaw C., Guèye A. C., Ndong A., Sarr M., Diop M. & Sembène M. (2015). Demographic and live cycle development comparison of three genetic populations of Tribolium castaneum Herbst (Coleoptera, Tenebrionidae). Journal of Applied Biosciences 93: 8727-8735.
[14] Excoffier L. & Heckel G. (2006). Computer programs for population genetics data analysis: a survival guide. Nat Rev Genet. 7: 745–758.
[15] Flores L. V., Gilardon E. & Gardenal, C. N. (2003). Genetic structure of populations of Tuta absoluta Meyrick (Lepidoptera: Gelechiidae). J. Basic Appl. Genet. 15: 29–32.
[16] Fu Y. X. (1997). Statistical tests of neutrality of mutation against population growth, hitch-hiking and background selection. Genetics 147(2): 915-925.
[17] Galarza J. (1984). Laboratory assessment of some solanaceous plants as possible food plants of the tomato moth Scrobipalpula absoluta. IDIANos421/424: 30-32.
[18] Guillemaud T., Blin A., Le Goff I., Desneux N., Reyes M., Tabone E., Tsagkarakou A., Nino L. & Lombaert E. (2015). The tomato borer, Tuta absoluta, invading the Mediterranean Basin, originates from a single introduction from Central Chile. Sci Rep. 5: 8371. https://doi.org/10.1038/ srep08371 PMid: 25667134 PMCid: PMC4322357.
[19] Hadapad A. B. & Hire R. S. (2019). Molecular characterisation of tomato leaf miner Tuta absoluta populations obtained from different geographical locations of India. Journal of Biological Control, 33(2): 147-154. DOI: 10.18311/jbc/2019/23115.
[20] Hall T. BioEdit version 5.0.6. Département of Micribiology (2001). North Carolina State University.
[21] Kafom A. C., Barry D., Diome T., Sarr A. G. R. J. & Sembène M. (2017). Agro-Ecological areas in Senegal affect the genetic structure of Callosobruchus maculatus F. The major pest of cowpea. International Journal of Biosciences 11(6): 116-129.
[22] Kiliç T. (2010). First record of Tuta absoluta in Turkey, Turkish Ministary of griculture and Rural Affairs, Plants Protection Rechearch Institute. Gençlik Caddesi no 6, Bornova 35040, Izmir, Turley, Phytoparasitica: 2p.
[23] Librado P. & Rozas J. (2009). DnaSP version v5: A software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25: 1451-1452.
[24] Naika S., De Jeude J. V. L., De Goffau M., Hilmi M., Van Dam B. & Florijn A. (2005). La culture de la tomate, production, transformation et commercialisation, cinquième édition révisée, Agromisa Foundation, coll. « Agrodok », Wageningen: 105 p.
[25] Ndiaye M. R. & Sembène M. (2018). Genetic structure and phylogeographic evolution of the West African populations of Sitophilus zeamais (Coleoptera, Curculionidae), Journal of Stored Products Research 77: 135-143.
[26] Notz A. P. (1992). Distribution of eggs and larvae of Scrobipalpula absoluta in potato plants. Revista de la Facultad de Agronomía (Maracay) 18: 425–432.
[27] Pfeiffer D. G., Muniappan R., Sall D., Diatta P., Diongue A. & Dieng E. O. (2013). First Record of Tuta Absoluta (lepidoptera: Gelechiidae) in Senegal. Fla. Entomol. 96: 661–662.
[28] Ramel J. M. & Oudard E. (2008). Tuta absoluta (Meyrick, 1917) éléments de reconnaissance. Fiche technique L. N. P. V. et S. R. P. V. Avignon Décembre 2008: 2p.
[29] Razuri V. & Vargas E. (1975). Biologiae comportamiento descrobical Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) en Tomate. Entomology. Peruana rev. 18: 84-89.
[30] Rozas J., Librado P., Sánchez-Del B. J. C., Messeguer X. & Rozas R. (2010). DnaSP Version 5 Help Contents [Help File], available with the program at http://www.ub.edu/dnasp/.
[31] Saitou N. & Nei M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology Evolution 4: 406-425.
[32] Shashank P. R, Twinkle S., Chandrashekar K., Meshram N. M., Suroshe, S. S. & Bajracharya, A. S. (2018). Genetic homogeneity in South American tomato pinworm, Tuta absoluta: a new invasive pest to oriental region. 3 Biotech 8(350): 1-7. https://doi.org/10.1007/s13205-018-1374-0
[33] Sembène M. (2000). Variabilité de l’Espaceur Interne Transcrit (ITS) de l’ADN ribosomique et polymorphisme des locus microsatellites chez la bruche Caryedon serratus (Olivier): différenciation en races hôtes et infestation de l’arachide au Sénégal. Thèse de Doctorat d’Etat ès Sciences, Université Cheikh Anta Diop de Dakar (Sénégal): 180p.
[34] Tamura K., Peterson D., Peterson N., Stecher G., Nei M. & Kumar S. (2011). Molecular Biology and Evolution. 28(10): 2731-2739.
[35] Trichpoulou A. & Lagio P. (1997). Healthy traditional Mediterranean diet: an expression culture, history and lifesly: 65p.
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    Mamecor Faye, Toffène Diome, Adja Rokhaya Diallo, Mbacké Sembène. (2023). Identification and Genetic Characterisation of Populations of Tuta absoluta (Meyrick, 1917; Lepidoptera: Gelechidae) Destroyer of Tomato Farming in Senegal. International Journal of Genetics and Genomics, 11(3), 104-111. https://doi.org/10.11648/j.ijgg.20231103.17

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    ACS Style

    Mamecor Faye; Toffène Diome; Adja Rokhaya Diallo; Mbacké Sembène. Identification and Genetic Characterisation of Populations of Tuta absoluta (Meyrick, 1917; Lepidoptera: Gelechidae) Destroyer of Tomato Farming in Senegal. Int. J. Genet. Genomics 2023, 11(3), 104-111. doi: 10.11648/j.ijgg.20231103.17

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    AMA Style

    Mamecor Faye, Toffène Diome, Adja Rokhaya Diallo, Mbacké Sembène. Identification and Genetic Characterisation of Populations of Tuta absoluta (Meyrick, 1917; Lepidoptera: Gelechidae) Destroyer of Tomato Farming in Senegal. Int J Genet Genomics. 2023;11(3):104-111. doi: 10.11648/j.ijgg.20231103.17

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  • @article{10.11648/j.ijgg.20231103.17,
      author = {Mamecor Faye and Toffène Diome and Adja Rokhaya Diallo and Mbacké Sembène},
      title = {Identification and Genetic Characterisation of Populations of Tuta absoluta (Meyrick, 1917; Lepidoptera: Gelechidae) Destroyer of Tomato Farming in Senegal},
      journal = {International Journal of Genetics and Genomics},
      volume = {11},
      number = {3},
      pages = {104-111},
      doi = {10.11648/j.ijgg.20231103.17},
      url = {https://doi.org/10.11648/j.ijgg.20231103.17},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijgg.20231103.17},
      abstract = {In Senegal tomato jeopardiser Tuta absoluta (Meyrick 1917; Lepidoptera: Gelechiidae) causes serious losses in tomato production. This species can feed on all the green parts of the tomato and can cause up to 100% damage if not controlled. Currently, the most effective method of pest management is the use of chemicals. However, as it is generally noticed, excessive and unconscious use of chemicals leads to very harmful environmental pollution. The objective of this study is to identify the haplotypes of Tuta absoluta but also to see if agro-ecological zones and localities have contributed to genetically structure its populations. To achieve this goal, we adopted the PCR-Sequencing technique of the mitochondrial gene Cytochrome B of three populations of T. absoluta from three different localities in Senegal. The study has revealed the presence of 8 haplotypes and a population of Tuta absoluta in demographic balance. No significant genetic structuring has been noted between agro-ecological zones or localities. Moreover, phylogenetic reconstructions show no clustering either according to agro-ecological zones (Niayes and the Senegal River Valley), or according to localities. Nevertheless two clades were revealed by the latter, suggesting a multiple introduction of T. absoluta followed by an expansion to different geographical regions of the country by diffusion after its introduction.},
     year = {2023}
    }
    

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  • TY  - JOUR
    T1  - Identification and Genetic Characterisation of Populations of Tuta absoluta (Meyrick, 1917; Lepidoptera: Gelechidae) Destroyer of Tomato Farming in Senegal
    AU  - Mamecor Faye
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    T2  - International Journal of Genetics and Genomics
    JF  - International Journal of Genetics and Genomics
    JO  - International Journal of Genetics and Genomics
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    EP  - 111
    PB  - Science Publishing Group
    SN  - 2376-7359
    UR  - https://doi.org/10.11648/j.ijgg.20231103.17
    AB  - In Senegal tomato jeopardiser Tuta absoluta (Meyrick 1917; Lepidoptera: Gelechiidae) causes serious losses in tomato production. This species can feed on all the green parts of the tomato and can cause up to 100% damage if not controlled. Currently, the most effective method of pest management is the use of chemicals. However, as it is generally noticed, excessive and unconscious use of chemicals leads to very harmful environmental pollution. The objective of this study is to identify the haplotypes of Tuta absoluta but also to see if agro-ecological zones and localities have contributed to genetically structure its populations. To achieve this goal, we adopted the PCR-Sequencing technique of the mitochondrial gene Cytochrome B of three populations of T. absoluta from three different localities in Senegal. The study has revealed the presence of 8 haplotypes and a population of Tuta absoluta in demographic balance. No significant genetic structuring has been noted between agro-ecological zones or localities. Moreover, phylogenetic reconstructions show no clustering either according to agro-ecological zones (Niayes and the Senegal River Valley), or according to localities. Nevertheless two clades were revealed by the latter, suggesting a multiple introduction of T. absoluta followed by an expansion to different geographical regions of the country by diffusion after its introduction.
    VL  - 11
    IS  - 3
    ER  - 

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Author Information
  • Laboratory of Parasitology, Department of Animal Biology, Faculty of Sciences and Techniques, Cheikh Anta Diop University of Dakar, Senegal

  • Genetic Team and Population Management, Department of Animal Biology, Faculty of Sciences and Techniques, Cheikh Anta Diop University of Dakar, Senegal

  • Genetic Team and Population Management, Department of Animal Biology, Faculty of Sciences and Techniques, Cheikh Anta Diop University of Dakar, Senegal

  • Genetic Team and Population Management, Department of Animal Biology, Faculty of Sciences and Techniques, Cheikh Anta Diop University of Dakar, Senegal

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