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Molecular Characterization of Imazaquin Tolerant and Sensitive Cowpea Genotypes

Received: 20 March 2016     Accepted: 1 April 2016     Published: 16 April 2016
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Abstract

In this paper, to investigate the tolerance of some cowpea genotypes to imazaquin, seeds of 30 cowpea genotypes were treated with imazaquin at 0.06 kg active ingredient ha-1 and control were treated with distilled water. Treated seeds were planted in plastic pots and raised for 3 weeks in a green house. Samples leaves were detached for DNA isolation at 2 weeks after sowing. Phenotypic result revealed that Hen-me and Hodi were the most tolerant (with 12.5% mortality rate) to imazaquin while Maptwapa and many others (with 100% mortality rate) were highly sensitive to imazaquin. However, there were no sequences differences between tolerant and sensitive genotypes in amplified region of the annotated portion of AHAS (acetohydroxy-acid synthase) from cowpea. It can be concluded that the major significant difference between imazaquin tolerance and sensitive cowpea is as a result of rapid metabolic detoxification of herbicides in tolerant cowpea. It is therefore imperative that enzymology involve in the differential metabolism of this herbicide in this crop needs urgent and necessary attention.

Published in International Journal of Genetics and Genomics (Volume 4, Issue 2)
DOI 10.11648/j.ijgg.20160402.11
Page(s) 5-10
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

AHAS Enzyme, Imazaquin, Herbicide Tolerance and Cowpea Genotypes

References
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[3] Barrett M. (1989). Reduction of imazaquin injury to corn (Zea mays) and sorghum (Sorghum bicolor) with antidotes. Weed Science, 37: 34-41.
[4] Congleton W. F., Vancantfort A. M. and Lignowski E. (1987). Imazaquin (scepter): A new soybean herbicide. Weed Technology, 1 (2): 186-188.
[5] Chen X. Laudeman T. W. Rushton P. J. Spraggins T. A. and Timko M. P. (2007). CGKB: An annotation knowledge base for cowpea (Vigna unguiculata L.) Methylation filtered genomic gene space sequences. BMC Bioinformatic, http://ww.biomedcentral.com/1471 21058/129.
[6] Li C., Fatokun C. A., Ubi B., Singh B. B. and Scoles G. J. (2001). Determining genetic similarities and relationships among cowpea breeding lines and cultivars by microsatellite markers. Crop Science, 31(1): 189-197.
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[8] Riskey, M. A. and Lawrence O. L (1991). Efficacy of Imazaquin on various weed species. Weed Science 39 (2): 243-250.
[9] Tecle, B. Cunha D. A. and Shaner L. D. (1993). Differential routes of metabolism of imidazolinones: Basis for soybean (Glycine max) selectivity. Pesticide Biochemistry and Physiology 46: 120-130.
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[16] Berner D. K., Award A. E. and Aigbokhan E. I. (1994). Potentials of imazaquin seed treatments for control of Striga gesnerioides and Alectra vogelii in cowpea (Vigna unguiculata). Plant Disease, 7: 18-23.
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Cite This Article
  • APA Style

    Abdulrahman Lado, Muhammad Auwal Hussaini, Alpha Yaya Kamara. (2016). Molecular Characterization of Imazaquin Tolerant and Sensitive Cowpea Genotypes. International Journal of Genetics and Genomics, 4(2), 5-10. https://doi.org/10.11648/j.ijgg.20160402.11

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    ACS Style

    Abdulrahman Lado; Muhammad Auwal Hussaini; Alpha Yaya Kamara. Molecular Characterization of Imazaquin Tolerant and Sensitive Cowpea Genotypes. Int. J. Genet. Genomics 2016, 4(2), 5-10. doi: 10.11648/j.ijgg.20160402.11

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    AMA Style

    Abdulrahman Lado, Muhammad Auwal Hussaini, Alpha Yaya Kamara. Molecular Characterization of Imazaquin Tolerant and Sensitive Cowpea Genotypes. Int J Genet Genomics. 2016;4(2):5-10. doi: 10.11648/j.ijgg.20160402.11

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  • @article{10.11648/j.ijgg.20160402.11,
      author = {Abdulrahman Lado and Muhammad Auwal Hussaini and Alpha Yaya Kamara},
      title = {Molecular Characterization of Imazaquin Tolerant and Sensitive Cowpea Genotypes},
      journal = {International Journal of Genetics and Genomics},
      volume = {4},
      number = {2},
      pages = {5-10},
      doi = {10.11648/j.ijgg.20160402.11},
      url = {https://doi.org/10.11648/j.ijgg.20160402.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20160402.11},
      abstract = {In this paper, to investigate the tolerance of some cowpea genotypes to imazaquin, seeds of 30 cowpea genotypes were treated with imazaquin at 0.06 kg active ingredient ha-1 and control were treated with distilled water. Treated seeds were planted in plastic pots and raised for 3 weeks in a green house. Samples leaves were detached for DNA isolation at 2 weeks after sowing. Phenotypic result revealed that Hen-me and Hodi were the most tolerant (with 12.5% mortality rate) to imazaquin while Maptwapa and many others (with 100% mortality rate) were highly sensitive to imazaquin. However, there were no sequences differences between tolerant and sensitive genotypes in amplified region of the annotated portion of AHAS (acetohydroxy-acid synthase) from cowpea. It can be concluded that the major significant difference between imazaquin tolerance and sensitive cowpea is as a result of rapid metabolic detoxification of herbicides in tolerant cowpea. It is therefore imperative that enzymology involve in the differential metabolism of this herbicide in this crop needs urgent and necessary attention.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - Molecular Characterization of Imazaquin Tolerant and Sensitive Cowpea Genotypes
    AU  - Abdulrahman Lado
    AU  - Muhammad Auwal Hussaini
    AU  - Alpha Yaya Kamara
    Y1  - 2016/04/16
    PY  - 2016
    N1  - https://doi.org/10.11648/j.ijgg.20160402.11
    DO  - 10.11648/j.ijgg.20160402.11
    T2  - International Journal of Genetics and Genomics
    JF  - International Journal of Genetics and Genomics
    JO  - International Journal of Genetics and Genomics
    SP  - 5
    EP  - 10
    PB  - Science Publishing Group
    SN  - 2376-7359
    UR  - https://doi.org/10.11648/j.ijgg.20160402.11
    AB  - In this paper, to investigate the tolerance of some cowpea genotypes to imazaquin, seeds of 30 cowpea genotypes were treated with imazaquin at 0.06 kg active ingredient ha-1 and control were treated with distilled water. Treated seeds were planted in plastic pots and raised for 3 weeks in a green house. Samples leaves were detached for DNA isolation at 2 weeks after sowing. Phenotypic result revealed that Hen-me and Hodi were the most tolerant (with 12.5% mortality rate) to imazaquin while Maptwapa and many others (with 100% mortality rate) were highly sensitive to imazaquin. However, there were no sequences differences between tolerant and sensitive genotypes in amplified region of the annotated portion of AHAS (acetohydroxy-acid synthase) from cowpea. It can be concluded that the major significant difference between imazaquin tolerance and sensitive cowpea is as a result of rapid metabolic detoxification of herbicides in tolerant cowpea. It is therefore imperative that enzymology involve in the differential metabolism of this herbicide in this crop needs urgent and necessary attention.
    VL  - 4
    IS  - 2
    ER  - 

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Author Information
  • Department of Agronomy, Bayero University, Kano, Nigeria

  • Department of Agronomy, Bayero University, Kano, Nigeria

  • International Institute of Tropical Agriculture, Kano Station, Nigeria

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