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Microbiological Assessment of Ready-to-Eat Food Sold in Urban Primary Schools, Douala, Littoral Region, Cameroon

Received: 15 June 2020     Accepted: 29 June 2020     Published: 21 May 2021
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Abstract

Ready-to-eat (RTE) food sold to school going children at school premises can not only provide essential nutrient and energy but also a route for foodborne diseases (FBD). However, there is a paucity of data on microbiological assessment of RTE food sold in primary schools in Douala. The aims of this study was to investigate the microbiological quality of food sold in primary schools and to evaluated the food safety knowledge and practice of vendors to promote a safer school-based food for a better health and well-being of our children. Socio-demographic characteristics and the hygienic practices of 60 RTE food vendors were collected using structured questionnaire. A total of 60 food samples from three different food items (beans, spaghetti and meat) were analyzed for the presence of bacterial and fungal pathogens. Twenty-five grams of each food sample was transferred in to 225 ml of buffered peptone water and homogenized. The homogenates were serially dilute and a volume of 0.1 ml dilution was spread on solid media and incubated at 35-37°C for 24 hours and 5 days. Antibiotic susceptibility testing was done for isolated species using Muller Hinton agar and data was entered in excel and exported to SPSS version 20.0. For analysis. The overall prevalence of bacterial pathogens was 23.1% (26/60) and the total mean enterobacteriaceae count (MEC) was 8.203333x104 CFU/ml in which the value ranged from 3.4 x104–2.06 x 105 CFU/ml. The total mean fungal count (MFC) was 1.0341x105 CFU/g which varied from 0 –2.8x105 CFU/ml. Of the total of 60 samples examined, 93.3% (56/60) were found positive for S. aureus of which 15% (09/60) of isolates were contaminated. Citrobacter freundii was isolated in 23.4% (14/60) of RTE food. The greatest number of S. aureus was found in beans and the lowest number in spaghetti. These findings indicated that, the ready-to-eat food sold to primary school children in Douala metropolis represent an important potential health risk to school going children. There is a need for Public Health authority to establish guidelines and standards in order to safeguard the wellbeing of the school going children.

Published in International Journal of Food Science and Biotechnology (Volume 6, Issue 2)
DOI 10.11648/j.ijfsb.20210602.13
Page(s) 36-44
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Ready-to-eat, Microbiological Assessment, Food Vendors, Antibiotic Susceptibility, Douala

References
[1] World Health Organization. “WHO estimates of the global burden of foodborne diseases: foodborne disease burden epidemiology reference group 2007-2015”. 2015.
[2] Daniyan, S. Y., and Nwokwu, O. E, “Enumeration of microorganisms associated with the different stages of bread production in Futmin bakery”, International Research of Pharmacy, (2). 88-91. 2011.
[3] Awol, N., Nigusse, D., and Ali, M, “Prevalence and antimicrobial susceptibility profile of Salmonella and Shigella among food handlers working in food establishment at Hawassa city, Southern Ethiopia,” BMC research Notes, (12). 7-12. 2019.
[4] Health Protection Agency. “Guidelines for Assessing the Microbiological Safety of Ready-to-Eat Foods”, London: Health Protection Agency, 2009.
[5] Addis, M., Sisay, D, “A Review on Major Food Borne Bacterial Illnesses”, Journal of Tropical Diseases, (3). 176. 2015.
[6] Jéssica, A. F. F. F., Wilma, S. G. V. B., Daniele, F. M., Deborah, H. M. B., and Uelinton, M. P, “Overview of Foodborne Disease Outbreaks in Brazil from 2000 to 2018”, Foo5s, (8), 434, 2019.
[7] Shonhiwa, A. M., Ntshoe, G., Essel, V., Thomas, J., McCarthy, K, “A review of foodborne diseases outbreaks reported to the outbreak response unit, national institute for communicable diseases, South Africa, 2013–2017” Abstracts / International Journal of Infectious Diseases, 79 (S1), 1–150. 2019.
[8] Food and Agriculture Organization of the United Nations “Nutrition guidelines and standards for school meals A report from 33 low and middle-income countries” Rome, 2019.
[9] Pouokam, G. B., Foudjo, B. U. S., Samuel, C., Yamgai, P. F., Silapeux, A. K., Sando, J. T., Atonde, G. F. and Frazzoli, C, “Contaminants in Foods of Animal Origin in Cameroon: A One Health Vision for Risk Management “from Farm to Fork” ”. frontiers in Public Health, (5). 197. 2017.
[10] Assob, J. C. N., Nde P. F., Nsagha, D. S., Njimoh, D. L., Nfor, O., Njunda, A. L., Kamga, H. L. F, “The incidence of feco-oral parasites in street-food vendors in Buea, south-west region Cameroon” African Health Sciences, (12), 2012.
[11] Akoachere, J F. T. K., Tatsinkou, B. F., and Nkengfack, J. M, “Bacterial and parasitic contaminants of salad vegetables sold in markets in Fako Division, Cameroon and evaluation of hygiene and handling practices of vendors” BMC Research Notes, (11).100. 2018.
[12] Kabiru, M. R., And Madaki, H. M, “Assessment of the microbiological quality of some locally made candies sold at some primary schools at Sharada, Bayero” Journal of Pure and Applied Sciences, (1). 10. 285-289. 2017.
[13] Bergey’s manual of determinative bacteriology, 9th edition
[14] Sharma, I. and Mazumdar, J. A, “Assessment of bacteriological quality of ready to eat food vended in streets of Silchar city, Assam, India”, Indian journal of medical microbiology, 32 (2), 169-171. 2014.
[15] World Health Organization “Food Safety: What you should know”. Regional Office for South-East Asia, Indraprastha Estate, Mahatma Gandhi Marg, New Delhi 110 002, India, 2015.
[16] Galgamuwa, LS., Iddawela, D., Dharmaratne, S. D, “Knowledge and practices of food hygiene among food handlers in plantation sector, Sri Lanka” International Journal of Scientific Reports, 2 (12), 304-311. 2016.
[17] Amare, A., Worku, T., Ashagirie, B., Adugna, M., Getaneh, A, “Bacteriological profile, antimicrobial susceptibility patterns of the isolates among street vended foods and hygienic practice of vendors in Gondar town, Northwest Ethiopia: a cross sectional study” BMC Microbiology, (19), 120. 2019.
[18] Tuladhar, R. and Singh A, “Bacterial Analysis Food of Kathmandu” Journal Natural History Museum, (26), 1-9 2012.
[19] Afolabi, O. R., Oloyede, A. R., Obuotor, T. M., Adegoke, L. M., Adeleke, A. O., Komonibo, T. O, “Microbiological Safety of Ready-to-eat Foods Sold in Primary Schools in Abeokuta, South-West Nigeria”, Journal of Science & Sustainable Development, (5), 65-73. 2012.
[20] Bereda, T. W., Emerie, Y. M., Reta, M. A., Asfaw, H. S, “Microbiological safety of street vended foods in Jigjiga City, eastern Ethiopia”. Ethiopian Journal of Health Science. 26 (2): 163– 72. 2016.
[21] Sileshi, S, “Bacteriological quality of food in Arba Minch University, Abaya Campus Student’s Cafeteria”. International Research Journal of biological science. 7 (1), 6-14, 2018.
[22] International Commission for Microbiological Specification in Food (ICMSF) “Microorganisms in foods. 7. Microbiological Testing in Food Safety Management”. Kluwer Academic/Plenum Publishers, New York, USA. 20-40. 2002.
[23] Reis, T. V. M., Coutinho de Oliveira, T. L., Andrade Boari, C. and Hilsdorf P. R, “Isolamento de coliformes, estafilococos e enterococos de leite cru provenientes de tanques de refrigeração por expansão comunitários: identificação, ação lipolítica e proteolítica”. Ciência e Tecnologia de Alimentos, 28 (3), 753-760. 2008.
[24] Githaiga GM. “Microbial quality, strain distribution and Enterotoxigenicity of selected food borne pathogens in relation to the hygienic practices in industrial area, Nairobi, Kenya”: Faculty of Agriculture, College of Agriculture and Veterinary Sciences, University of Nairobi; 2012.
[25] Akusu, O., Kiin-Kabari, D., Wemedo, S, “Microbiological quality of selected street vended foods in Port Harcourt metropolis, Rivers state, Nigeria”. Sky Journal of Food Science, 5 (2). 08–11.2016.
[26] Kwiri, R., Winini, C., Tongonya, J., Gwala, W., Mpofu, E., Mujuru, F, “Microbiological safety of cooked vended foods in an urban informal market: a case study of Mbare Msika, Harare, Zimbabwe”. 2014.
[27] Wang, J. T, Chang, S. C., Chen, Y. C., and Luh, K. T, “Comparison of antimicrobial susceptibility of Citrobacter freundii isolates in two different time periods.” The Journal of Microbiology, Immunology and Infection. 33 (4): 258-262. 2000.
[28] Chen, H., Wang, Y., Zhang, J., Yunsheng, C., Minglin, W, “Isolation and identifiation of Citrobacter spp. from the intestine of Procambarus clarkii”. Journal of Fisheries Research, 2 (1): 1-6. 2017.
[29] Bai, L., Xia, S., Lan, R., Lui, L., Yel, C., Wang, Y, Isolation and characterization of cytotoxic, aggregative Citrobacter freundii. PLoS One, 7 (3): e33054. 2012.
[30] Trofa, D., Ga´cser, A., and Nosanchuk, J. D, “Candida parapsilosis, an Emerging Fungal Pathogen”, Clinical Microbiology Reviews, 606–625, 2008.
[31] Garedew-Kifelew, L., Wondafrash, N., Feleke, A, “Identifcation of drug-resistant Salmonella from food handlers at the University of Gondar, Ethiopia”, BMC Research Notes. (7), 545. 2014.
[32] Mohanty, S., Singhal, R., Sood, S., Dhawan, B., Kapil, A., Das, B. K, “Citrobacter infections in a tertiary care hospital in Northern India. Journal of Infectious”, 54 (1).58e64. 2007.
[33] Liu, L-H., Wang, N-Y., A Ying-Jung, A. W., Lin, C-C., Lee, C-M., Liu, C-P, “Citrobacter freundii bacteremia: Risk factors of mortality and prevalence of resistance genes”, Journal of Microbiology, Immunology and Infection, (51), 565e572. 2018.
[34] Liu, L., Lan, R., Liu, L., Wang, Y., Zhang, Y., Wang, Y. and Xu, J. “Antimicrobial Resistance and Cytotoxicity of Citrobacter spp. in Maanshan Anhui Province, China”. Frontiers in Microbiology, (8). 1357. 2017.
Cite This Article
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    Ariane Laure Wounang Ngueugang, Bonaventure Tientche, Smith Asaah, Henri Lucien Fouamno Kamga, Gertrude Bsadjo Tchamba. (2021). Microbiological Assessment of Ready-to-Eat Food Sold in Urban Primary Schools, Douala, Littoral Region, Cameroon. International Journal of Food Science and Biotechnology, 6(2), 36-44. https://doi.org/10.11648/j.ijfsb.20210602.13

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    ACS Style

    Ariane Laure Wounang Ngueugang; Bonaventure Tientche; Smith Asaah; Henri Lucien Fouamno Kamga; Gertrude Bsadjo Tchamba. Microbiological Assessment of Ready-to-Eat Food Sold in Urban Primary Schools, Douala, Littoral Region, Cameroon. Int. J. Food Sci. Biotechnol. 2021, 6(2), 36-44. doi: 10.11648/j.ijfsb.20210602.13

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    AMA Style

    Ariane Laure Wounang Ngueugang, Bonaventure Tientche, Smith Asaah, Henri Lucien Fouamno Kamga, Gertrude Bsadjo Tchamba. Microbiological Assessment of Ready-to-Eat Food Sold in Urban Primary Schools, Douala, Littoral Region, Cameroon. Int J Food Sci Biotechnol. 2021;6(2):36-44. doi: 10.11648/j.ijfsb.20210602.13

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  • @article{10.11648/j.ijfsb.20210602.13,
      author = {Ariane Laure Wounang Ngueugang and Bonaventure Tientche and Smith Asaah and Henri Lucien Fouamno Kamga and Gertrude Bsadjo Tchamba},
      title = {Microbiological Assessment of Ready-to-Eat Food Sold in Urban Primary Schools, Douala, Littoral Region, Cameroon},
      journal = {International Journal of Food Science and Biotechnology},
      volume = {6},
      number = {2},
      pages = {36-44},
      doi = {10.11648/j.ijfsb.20210602.13},
      url = {https://doi.org/10.11648/j.ijfsb.20210602.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijfsb.20210602.13},
      abstract = {Ready-to-eat (RTE) food sold to school going children at school premises can not only provide essential nutrient and energy but also a route for foodborne diseases (FBD). However, there is a paucity of data on microbiological assessment of RTE food sold in primary schools in Douala. The aims of this study was to investigate the microbiological quality of food sold in primary schools and to evaluated the food safety knowledge and practice of vendors to promote a safer school-based food for a better health and well-being of our children. Socio-demographic characteristics and the hygienic practices of 60 RTE food vendors were collected using structured questionnaire. A total of 60 food samples from three different food items (beans, spaghetti and meat) were analyzed for the presence of bacterial and fungal pathogens. Twenty-five grams of each food sample was transferred in to 225 ml of buffered peptone water and homogenized. The homogenates were serially dilute and a volume of 0.1 ml dilution was spread on solid media and incubated at 35-37°C for 24 hours and 5 days. Antibiotic susceptibility testing was done for isolated species using Muller Hinton agar and data was entered in excel and exported to SPSS version 20.0. For analysis. The overall prevalence of bacterial pathogens was 23.1% (26/60) and the total mean enterobacteriaceae count (MEC) was 8.203333x104 CFU/ml in which the value ranged from 3.4 x104–2.06 x 105 CFU/ml. The total mean fungal count (MFC) was 1.0341x105 CFU/g which varied from 0 –2.8x105 CFU/ml. Of the total of 60 samples examined, 93.3% (56/60) were found positive for S. aureus of which 15% (09/60) of isolates were contaminated. Citrobacter freundii was isolated in 23.4% (14/60) of RTE food. The greatest number of S. aureus was found in beans and the lowest number in spaghetti. These findings indicated that, the ready-to-eat food sold to primary school children in Douala metropolis represent an important potential health risk to school going children. There is a need for Public Health authority to establish guidelines and standards in order to safeguard the wellbeing of the school going children.},
     year = {2021}
    }
    

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  • TY  - JOUR
    T1  - Microbiological Assessment of Ready-to-Eat Food Sold in Urban Primary Schools, Douala, Littoral Region, Cameroon
    AU  - Ariane Laure Wounang Ngueugang
    AU  - Bonaventure Tientche
    AU  - Smith Asaah
    AU  - Henri Lucien Fouamno Kamga
    AU  - Gertrude Bsadjo Tchamba
    Y1  - 2021/05/21
    PY  - 2021
    N1  - https://doi.org/10.11648/j.ijfsb.20210602.13
    DO  - 10.11648/j.ijfsb.20210602.13
    T2  - International Journal of Food Science and Biotechnology
    JF  - International Journal of Food Science and Biotechnology
    JO  - International Journal of Food Science and Biotechnology
    SP  - 36
    EP  - 44
    PB  - Science Publishing Group
    SN  - 2578-9643
    UR  - https://doi.org/10.11648/j.ijfsb.20210602.13
    AB  - Ready-to-eat (RTE) food sold to school going children at school premises can not only provide essential nutrient and energy but also a route for foodborne diseases (FBD). However, there is a paucity of data on microbiological assessment of RTE food sold in primary schools in Douala. The aims of this study was to investigate the microbiological quality of food sold in primary schools and to evaluated the food safety knowledge and practice of vendors to promote a safer school-based food for a better health and well-being of our children. Socio-demographic characteristics and the hygienic practices of 60 RTE food vendors were collected using structured questionnaire. A total of 60 food samples from three different food items (beans, spaghetti and meat) were analyzed for the presence of bacterial and fungal pathogens. Twenty-five grams of each food sample was transferred in to 225 ml of buffered peptone water and homogenized. The homogenates were serially dilute and a volume of 0.1 ml dilution was spread on solid media and incubated at 35-37°C for 24 hours and 5 days. Antibiotic susceptibility testing was done for isolated species using Muller Hinton agar and data was entered in excel and exported to SPSS version 20.0. For analysis. The overall prevalence of bacterial pathogens was 23.1% (26/60) and the total mean enterobacteriaceae count (MEC) was 8.203333x104 CFU/ml in which the value ranged from 3.4 x104–2.06 x 105 CFU/ml. The total mean fungal count (MFC) was 1.0341x105 CFU/g which varied from 0 –2.8x105 CFU/ml. Of the total of 60 samples examined, 93.3% (56/60) were found positive for S. aureus of which 15% (09/60) of isolates were contaminated. Citrobacter freundii was isolated in 23.4% (14/60) of RTE food. The greatest number of S. aureus was found in beans and the lowest number in spaghetti. These findings indicated that, the ready-to-eat food sold to primary school children in Douala metropolis represent an important potential health risk to school going children. There is a need for Public Health authority to establish guidelines and standards in order to safeguard the wellbeing of the school going children.
    VL  - 6
    IS  - 2
    ER  - 

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Author Information
  • Estuary Academic and Strategic Institute (IUEs/INSAM), Douala, Cameroon

  • Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon

  • Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon

  • Department of Medical Laboratory Science, University of Bamenda, Bambili, Cameroon

  • Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon

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