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Determination of the Concentration of Polyamines with SPR-Based Immune Biosensor for Early Diagnostics of Breast Cancer

Received: 13 November 2016     Accepted: 3 December 2016     Published: 17 January 2017
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Abstract

The paper presents the results of research on the development of immune biosensor test system for express detection of polyamines in cells of breast cancer. Determination of polyamines was performed by using an analytical device - immune biosensor based on surface plasmon resonance (SPR), where "antigen-antibody" reaction is performed in real time on the surface of transducer, resulting in formation of immune complexes and recording the shift of resonance angle. It was performed two methods of analysis: direct, when immobilized specific antibodies interacted with appropriate antigens and competitive, when the one part of antigens was conjugated with protein and competed for binding with specific antibodies with free antigens. In relation to direct analysis it was shown the dependence of sensitivity of biosensor response from the concentration of polyamines in range of 10-100 ng /ml. Concerning the competitive method of analysis, the linear region of sensitivity was in range of 0,3-80 ng/ml and 0,01-90 ng/ml for spermine and spermidine respectively.

Published in International Journal of Clinical Oncology and Cancer Research (Volume 1, Issue 1)
DOI 10.11648/j.ijcocr.20160101.15
Page(s) 30-35
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

SPR, Polyamines, Antibody, Antigen, Surface Plasmon Resonance, Breast Cancer

References
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[2] S. P. Osynskyi Metabolic environment of cancer cells., Oncology, 2010, pp. 197–218.
[3] Qi С, Gao G, Jin G. Label-free Biosensors for Health Applications. In book “Biosensors for Health, Environment and Biosecurity”, edited by Pier Andrea Serra. InTech 2011, 550 p.
[4] N. F. Starodub. Efficiency of Biosensors in Environmental Monitoring. Book of SERIES IN SENSORS, Portable Biosensing of Food Toxicants and Environmental Pollutants, CRC Press, Taylor & Francis Croup Boca Raton London, NewYork, 2013, pp. 515-560.
[5] N. S. Sergeeva,, N. V. Marshutina. The general idea of the serologic biomarkers and their place in oncology., Practical Oncology, vol. 12, 4, 2011, pp. 147-154.
[6] V. F Suhoy, V. S. Pervyi, N. V. Suhaya. Tumor markers in clinical practice: Handbook for doctors., Dnepropetrovsk: ART Press, 2003. p. 44.
[7] A. Gugliucci. Polyamines as clinical laboratory tools., Clinica chimica acta 344.1, 2004, 23-35.
[8] M. H. Zhang, T. Caragine, HC Wang, PS Cohen, G. Botchkina, K. Soda, M. Bianchi, P. Ulrich, A. Cerami, B. Sherry, KJ Tracey J. Exp. Med., 185, 1997, p. 1759.
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[11] D. M. L. Morgan Biochem. Soc. Trans., 18, 1990, p. 1080.
[12] D. H. Russell Nat. New Biol., 233, 1971, p. 144.
[13] T. Takayama et al. Diagnostic approach to breast cancer patients based on target metabolomics in saliva by liquid chromatography with tandem mass spectrometry Clinica Chimica Acta, 452, 2016, pp. 18–26.
[14] L. D Voronchihina, V. T Demyanova, S. A Sitnikov. Polyamine concentration in the blood of healthy people. Problems of Medical Chemistry., vol 22., 2., 1986, pp. 43-45.
[15] D. H. Russell, S. H. Snyder, Amine synthesis in rapidly growing tissues: ornithine decarboxylase activity in regenerating rat liver, chick embryo, and various tumors, Proc. Natl. Acad. Sci. 60, 1968, pp. 1420–1427.
[16] J. W. Suh, S. H. Lee, B. C. Chung, J. Park, Urinary polyamine evaluation for effective diagnosis of various cancers, J Chromatogr. B 688, 1997, pp. 179–186.
[17] S. H. Lee, S. O. Kim, H. Lee, B. C. Chung, Estrogens and polyamines in breast cancer: their profiles and values in disease staging, Cancer Lett. 133, 1998, pp. 47–56.
[18] K. Hiramatsu, H. Miura, S. Kamei, K. Iwasaki, M. Kawakita, Development of a sensitive and accurate enzyme-linked immunosorbent assay (ELISA) system that can replace HPLC analysis for the determination of N1, N12-diacetylspermine in human urine, J. Biochem. 124,1998, pp. 231–236.
[19] D Bartos,., R. A Campbell,., F Bartos,., D. P Grettie,. Direct determination of polyamines in human serum by radioimmunoassay. J. Cancer Res. 35, 1975, pp. 2056-2060.
[20] Fujiwara, K et al.,. Preparation ofpolyamine antibody and its use in enzyme immunoassay of spermine and spermidine with `D-galactosidase as a label. J. Immunol. Methods 61, 1983, pp. 217-226.
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  • APA Style

    Maksym Prylutskyi, Nickolaj Starodub, Nadiia Bilko. (2017). Determination of the Concentration of Polyamines with SPR-Based Immune Biosensor for Early Diagnostics of Breast Cancer. International Journal of Clinical Oncology and Cancer Research, 1(1), 30-35. https://doi.org/10.11648/j.ijcocr.20160101.15

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    ACS Style

    Maksym Prylutskyi; Nickolaj Starodub; Nadiia Bilko. Determination of the Concentration of Polyamines with SPR-Based Immune Biosensor for Early Diagnostics of Breast Cancer. Int. J. Clin. Oncol. Cancer Res. 2017, 1(1), 30-35. doi: 10.11648/j.ijcocr.20160101.15

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    AMA Style

    Maksym Prylutskyi, Nickolaj Starodub, Nadiia Bilko. Determination of the Concentration of Polyamines with SPR-Based Immune Biosensor for Early Diagnostics of Breast Cancer. Int J Clin Oncol Cancer Res. 2017;1(1):30-35. doi: 10.11648/j.ijcocr.20160101.15

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  • @article{10.11648/j.ijcocr.20160101.15,
      author = {Maksym Prylutskyi and Nickolaj Starodub and Nadiia Bilko},
      title = {Determination of the Concentration of Polyamines with SPR-Based Immune Biosensor for Early Diagnostics of Breast Cancer},
      journal = {International Journal of Clinical Oncology and Cancer Research},
      volume = {1},
      number = {1},
      pages = {30-35},
      doi = {10.11648/j.ijcocr.20160101.15},
      url = {https://doi.org/10.11648/j.ijcocr.20160101.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcocr.20160101.15},
      abstract = {The paper presents the results of research on the development of immune biosensor test system for express detection of polyamines in cells of breast cancer. Determination of polyamines was performed by using an analytical device - immune biosensor based on surface plasmon resonance (SPR), where "antigen-antibody" reaction is performed in real time on the surface of transducer, resulting in formation of immune complexes and recording the shift of resonance angle. It was performed two methods of analysis: direct, when immobilized specific antibodies interacted with appropriate antigens and competitive, when the one part of antigens was conjugated with protein and competed for binding with specific antibodies with free antigens. In relation to direct analysis it was shown the dependence of sensitivity of biosensor response from the concentration of polyamines in range of 10-100 ng /ml. Concerning the competitive method of analysis, the linear region of sensitivity was in range of 0,3-80 ng/ml and 0,01-90 ng/ml for spermine and spermidine respectively.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Determination of the Concentration of Polyamines with SPR-Based Immune Biosensor for Early Diagnostics of Breast Cancer
    AU  - Maksym Prylutskyi
    AU  - Nickolaj Starodub
    AU  - Nadiia Bilko
    Y1  - 2017/01/17
    PY  - 2017
    N1  - https://doi.org/10.11648/j.ijcocr.20160101.15
    DO  - 10.11648/j.ijcocr.20160101.15
    T2  - International Journal of Clinical Oncology and Cancer Research
    JF  - International Journal of Clinical Oncology and Cancer Research
    JO  - International Journal of Clinical Oncology and Cancer Research
    SP  - 30
    EP  - 35
    PB  - Science Publishing Group
    SN  - 2578-9511
    UR  - https://doi.org/10.11648/j.ijcocr.20160101.15
    AB  - The paper presents the results of research on the development of immune biosensor test system for express detection of polyamines in cells of breast cancer. Determination of polyamines was performed by using an analytical device - immune biosensor based on surface plasmon resonance (SPR), where "antigen-antibody" reaction is performed in real time on the surface of transducer, resulting in formation of immune complexes and recording the shift of resonance angle. It was performed two methods of analysis: direct, when immobilized specific antibodies interacted with appropriate antigens and competitive, when the one part of antigens was conjugated with protein and competed for binding with specific antibodies with free antigens. In relation to direct analysis it was shown the dependence of sensitivity of biosensor response from the concentration of polyamines in range of 10-100 ng /ml. Concerning the competitive method of analysis, the linear region of sensitivity was in range of 0,3-80 ng/ml and 0,01-90 ng/ml for spermine and spermidine respectively.
    VL  - 1
    IS  - 1
    ER  - 

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Author Information
  • Department of Laboratory Diagnostics of Biological Systems, Center of Molecular and Cell Research of National University of Kyiv-Mohyla Academy, Kyiv, Ukraine

  • Department of Molecular Biology, Microbiology and Biosafety of National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine

  • Department of Laboratory Diagnostics of Biological Systems, Center of Molecular and Cell Research of National University of Kyiv-Mohyla Academy, Kyiv, Ukraine

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