| Peer-Reviewed

Toxoplasmosis Sero-Prevalence, Awareness and Risk Behavior Among Pregnant Women Following Antenatal Care in Asella Teaching and Referral Hospital, Asella, Ethiopia

Received: 21 August 2020     Accepted: 3 September 2020     Published: 19 September 2020
Views:       Downloads:
Abstract

Toxoplasmosis is caused by infection with the protozoan Toxoplasma gondii (T. gondii), an obligate intracellular parasite. The infections produced a wide range of clinical syndromes in humans, land and sea mammals, and various bird species. Toxoplasmosis is especially important among Immune-compromised patients and pregnant women. Infection of mothers during pregnancy by Toxoplasma gondii may have serious consequences for fetus ranging from miscarriage, central nervous system involvement, retinochoroditis, or at birth subclinical infection. Objectives: To determine Toxoplasmosis sero-prevalence, awareness and risk Behavior among pregnant women following antenatal care in Asella Teaching and Referral Hospital, Asella, Ethiopia. Methods: Institutional based quantitative cross sectional study design was conducted on sample size of 384 from December 1, 2018 to June 30, 2019. To collect the demographic and risk factor related data a pre-tested Structured, questionnaire was used. Serum sample, collected was tested for Toxoplasma Immunoglobulin G (IgG) and Toxoplasma Immunoglobulin M (IgM) antibodies using anti- IgG and IgM antibodies by Toxoplasma immune-chromatographic test (ICT) IgG-IgM test. To show association between the dependent and independent variables a bivariable and multivariable logistic regression model was applied allowing for p, 0.05 and the confidence interval 95%. Result: The overall sero- prevalence of T. gondii in the study area was 81.8%. Three hundred and fourteen (81.8%) of the pregnant women were IgG seropositive, fifty women (13%) were IgM seropositive. fifty women were positive for both IgG and IgM. None of the pregnant women were positive exclusively for IgG and IgM ani-bodies. eating raw meat (COR=3.480, 95%CI: 1.450-8.352; P=0.005, AOR=3.798, 95%CI: 1.249-11.550, P=0.19), using unpasteurized milk (COR=3.860, 95%CI: 2.118-7.037; P=0.000, AOR=3.907, 95% CI: 1.744-8.751, P=0.001), having three or more children (COR=2.194, 95%CI: 1.065-4.518; P=0.033) and Consumption of raw egg (COR=2.042, 95%CI: 1.049-3.974; P=0.036). Conclusion: The sero-prevalence of T. gondii antibodies was high among the pregnant women. Those who consumed raw meat and egg, unpasteurized milk and those who have three or more children were at higher risk of T. gondii infection. Hence, blood screening for Toxoplasmosis, health education and awareness creation among pregnant women should be done during antenatal follow up.

Published in International Journal of Biomedical Science and Engineering (Volume 8, Issue 3)
DOI 10.11648/j.ijbse.20200803.12
Page(s) 28-35
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Sero-prevalence, Pregnant Women, Risk Factors. T. gondii, Asella, Ethiopia

References
[1] Dubey JP. Toxoplasmosis of animals and humans. Boca Raton, NW: CRC Press; 2010.
[2] Hill DE, Dubey JP. Toxoplasma gondii prevalence in farm animals in the United States. Int Parasitol. 2013; 43: 107–13.
[3] Flegr J, Prandota J, Sovičková M, Israili ZH. Toxoplasmosis-a global threat. Correlation of latent toxoplasmosis with specific disease burden in a set of 88 countries. PLoS One. 2014; 9: 1–22.
[4] Tedla, Y., T. Shibre, O. Ali, G. Tadele, Y. Woldeamanuel, D. Asrat, A. Aseffa, W. Mihret, M. Abebe, A. Alem, A. Habte and G. Medhin, Serum antibodies to Toxoplasma gondii and Herpesvidae family viruses in individuals with schizophrenia and bipolar disorder: a case-control study. Ethiopian Medical Journal, 2011; 49 (3): 211-220.
[5] Aleme, H., G. Tilahun, D. Fekade, N. Berhe and G. Medhin, Sereoprevalence of Immunoglobulin-G and of Immunoglobulin-M Anti Toxoplasma gondii Antibodies in Human Immunodefiiency Virus Infection/Acquired Immunodefiiency Syndrome Patients at Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia. Journal of Infectious Disease and Therapy, 2013; 1: 119. doi: 10.4172/2332-0877.1000119.
[6] Gelaye, W., T. Kebede and A. Hailu, 2015. High prevalence of anti-toxoplasma antibodies and absence of Toxoplasma gondi infection risk factors among pregnant women attending routine antenatal care in two Hospitals of Addis Ababa, Ethiopia, International Journal of Infectious Diseases (2015), http://dx.doi.org/10.1016/j.ijid.2015.03.005.
[7] Tenter AM, Heckeroth AR, Weiss LM. Toxoplasma gondii from animals to humans. Int Journal of Parasitol. 2000; 30: 1217–1258.
[8] Holliman RE. Toxoplasmosis. In: Cook GC, Alimuddin Zumla, editors. Mansons tropical diseases, London: Saunders Elsevier; 2009. 1367–73.
[9] Zemene, E., D. Yewhalaw, S. Abera, T. Belay, A. Samuel and A. Zeynudin, Sero-prevalence of Toxoplasma gondii and associated risk factors among pregnant women in Jimma town, Southwestern Ethiopia. BMC Infectious Diseases, 2012; 12 (1): 1.
[10] Kula Jilo and Jemal Adem, Toxoplasmosis and its Current Status in Ethiopia: A Review, Global Veterinaria 17 (2): 135-141, 2016.
[11] Torgerson, PR, Mastroiacovo, P. The global burden of congenital toxoplasmosis: a systematic review. Geneva: Bull. World Health Organization; 2013.
[12] Negash, T., G. Tilahun and G. Medhin, Sero-prevalence of Toxoplasma Gondii in Adama Town, Ethiopia. East African Journal of Public Health, 2008; 5 (3): 211-214.
[13] Skariah, S., M. K. McIntyre and D. G. Mordue, Toxoplasma gondii: determinants of tachyzoite to bradyzoite Conversion. Parasitology Research, 2010; 107 (2): 253-260.
[14] Montoya JG, Remington JS. Management of Toxoplasma gondii infection during pregnancy. Clin Infect Dis. 2008; 47: 554–66.
[15] Singh S, Munawwar A, Rao S, Mehta S, Hazarika NK. Serologic prevalence of Toxoplasma gondii in Indian women of child bearing age and effects of social and environmental factors. PLoS Negl Trop Dis. 2014; 8: 1–9.
[16] Kulasena VA, Rajapakse RP, Dubey JP, Dayawansa PN, Premawansa S. Sero-prevalence of T. gondii in cats from Colombo, Sri Lanka. Journal of Parasitology. 2011; 97: 152.
[17] Dubey JP, Rajapakse RP, Ekanayake DK, Sreekumar C, Lehmann T. Isolation and molecular characterization of Toxoplasma gondii from chickens from Sri Lanka. Journal of Parasitol. 2005; 91: 1480–2.
[18] Teshale, S., A. Dumetre, M. L. Dardé, B. Merga and P. Dorchies, Serological survey of caprine toxoplasmosis in Ethiopia: prevalence and risk factors. Parasite, 2007; 14 (2): 155-159.
[19] Yohanes T, Zerdo Z, Chufamo N, Ashenafi Abossie A. Sero-prevalence and associated factors of toxoplasma gondii infection among pregnant women attending in antenatal Clinic of Arba Minch Hospital, South Ethiopia: cross sectional study. Transl Biomed. 2017; 8 (1): 105. https://doi.org/10.2167/2172-0479.1000105.
[20] Da Silva MG, Vinaud MC, de Castro AM. Prevalence of toxoplasmosis in pregnant women and vertical transmission of toxoplasma gondii in patients from basic units of health from Gurupi, Tocantins, Brazil, from 2012 to 2014. PLoS One. 2015; 10 (11): e0141700.
[21] Ayi I, Sowah AO, Blay EA, Suzuki T, Ohta N, Ayeh-Kumi PF. Toxoplasma gondii infections among pregnant women, children and HIV-seropositive persons in Accra, Ghana. Trop Med Health. 2016; 44 (1): 17.
[22] Mwambe B, Mshana SE, Kidenya BR, Massinde AN, Mazigo HD, Michael D, Majinge C, Groß U. Sero-prevalence and factors associated with toxoplasma gondii infection among pregnant women attending antenatal care in Mwanza, Tanzania. Parasit Vectors. 2013; 6 (1): 222.
[23] Iddawela et al., Sero-prevalence of toxoplasmosis and risk factors of Toxoplasma gondii infection among pregnant women in Sri Lanka: a cross sectional study. BMC Public Health (2017) 17: 930.
[24] Cong W, Dong XY, Meng QF, Zhou N, Wang XY, Huang SY, Zhu XQ, Qian AD. Toxoplasma gondii infection in pregnant women: a sero-prevalence and case-control study in Eastern China. Biomed Res Int. 2015; 2015.
[25] Alvarado-Esquivel C. del Carmen Terrones-Saldívar M, Hernández-Tinoco J, Muñoz-Terrones MD, Gallegos-González RO, et al. Seroepidemiology of toxoplasma gondii in pregnant women in Aguascalientes City, Mexico: a cross-sectional study. BMJ Open. 2016; 6 (7): e012409.
[26] Nisbet AI, Omuse G, Revathi G, Adam RD. Sero-prevalence data at a private teaching hospital in Kenya: An examination of Toxoplasma gondii, cytomegalovirus, rubella, hepatitis A, and Entamoeba histolytica. PloS one. 2018; 13 (10): e0204867.
[27] Abamecha F, Awel H. Sero-prevalence and risk factors of toxoplasma gondii infection in pregnant women following antenatal care at Mizan Aman general hospital, bench Maji zone (BMZ), Ethiopia. BMC Infect Dis. 2016; 16: 460. https://doi.org/10.1186/s12879-016-1806-6.
[28] W. Gelaye et al., High prevalence of anti-toxoplasma antibodies and absence of Toxoplasma gondii infection risk factors among pregnant women attending routine antenatal care in two Hospitals of Addis Ababa, Ethiopia. International Journal of Infectious Diseases 34 (2015) 41–45.
[29] Ouologuem DT, Djimde AA, Diallo N, Doumbo OK, Roos DS. Toxoplasma gondii sero-prevalence in Mali. Journal of Parasitol. 2013; 99: 371-4.
[30] Khademi et al.: Prevalence and Risk Factors of Toxoplasma gondii Infection among Pregnant Women in Hormozgan Province, South of Iran. Iran Journal of Parasitol: 2019; 14: 167-173.
[31] Zemene E, Yewhalaw D, Abera S, Belay T, Samuel A, Zeynudin A. Sero-prevalence of toxoplasma gondii and associated risk factors among pregnant women in Jimma town, southwestern Ethiopia. BMC Infect Dis. 2012; 12: 337.
[32] Wei Cong et al., Toxoplasma gondii Infection in Pregnant Women: A Sero-prevalence and Case-Control Study in Eastern China. BioMed Research International. Volume 2015, Article ID 170278, 6 pages http://dx.doi.org/10.1155/2015/170278.
[33] Sroka S, Bartelheimer N, Winter A, Heukelbach J, Ariza L, Ribeiro H, et al. Prevalence and risk factors of toxoplasmosis among pregnant women in Fortaleza, northeastern Brazil. Am Journal of Trop Med Hyg. 2010; 83: 528–3.
[34] VanWormer E, Fritz H, Shapiro K, Mazet JA, Conrad PA. Molecules to modeling: toxoplasma gondii oocysts at the human-animal-environment interface. Comp Immunol Microbiol Infect Dis. 2013; 36 (3): 217-31.
[35] Alsammani MA. Sero-epidemiology and risk factors for toxoplasma gondii among pregnant women in Arab and African countries. Journal of Parasit Dis. 2016; 40 (3): 569-79.
[36] Robert-Gangneux F, Dardé ML. Epidemiology of and diagnostic strategies for toxoplasmosis. Clin Microbiol Rev. 2012; 25 (2): 264-96.
[37] Fenat. Sero-prevalence of Toxoplasma gondii among pregnant women attending antenatal clinics at Hawassa University Comprehensive specialized and Yirgalem General Hospitals, in southern Ethiopia. BMC Infectious Diseases.2019; 19: 1050.
[38] Liu Q, Wei F, Gao S, Jiang L, Lian H, Yuan B, et al. Toxoplasma gondii infection in pregnant women in China. Trans R Soc Trop Med Hyg. 2009; 103: 162–6.
[39] Riyadh A. Alzaheb and Osama Al-Amer. The Sero-prevalence and Risk Factors of Toxoplasmosis among Female Undergraduate University Students in Saudi Arabia. Oman Medical Journal [2017], Vol. 32, No. 6: 486-491.
[40] Andiappan et al. Knowledge and practice on Toxoplasma infection in pregnant women from Malaysia, Philippines, and Thailand. Frontiersin Microbiology | Microbial Immunology. June 2014; Vol. 5, Article 291 | 1-8.
[41] Anubhuti, Radha Rani Roy, J. P. Mittra, Shoukat Jahan Begum. “Sero-prevalence of Toxoplasma Gondii in Spontaneous Abortions in Pregnant Women”. Journal of Evolution of Medical and Dental Sciences 2015; Vol. 4, Issue 39, May 14; Page: 6763-6768, DOI: 10.14260/jemds/2015/980.
[42] Kefale Awoke et al., Sero-prevalence and associated risk factors of Toxoplasma gondii infection among pregnant women attending antenatal care at Felege Hiwot Referral Hospital, northwest Ethiopia./Asian Pacific Journal of Tropical Medicine 2015; 8 (7): 549–554.
Cite This Article
  • APA Style

    Shimeles Adugna Elemo. (2020). Toxoplasmosis Sero-Prevalence, Awareness and Risk Behavior Among Pregnant Women Following Antenatal Care in Asella Teaching and Referral Hospital, Asella, Ethiopia. International Journal of Biomedical Science and Engineering, 8(3), 28-35. https://doi.org/10.11648/j.ijbse.20200803.12

    Copy | Download

    ACS Style

    Shimeles Adugna Elemo. Toxoplasmosis Sero-Prevalence, Awareness and Risk Behavior Among Pregnant Women Following Antenatal Care in Asella Teaching and Referral Hospital, Asella, Ethiopia. Int. J. Biomed. Sci. Eng. 2020, 8(3), 28-35. doi: 10.11648/j.ijbse.20200803.12

    Copy | Download

    AMA Style

    Shimeles Adugna Elemo. Toxoplasmosis Sero-Prevalence, Awareness and Risk Behavior Among Pregnant Women Following Antenatal Care in Asella Teaching and Referral Hospital, Asella, Ethiopia. Int J Biomed Sci Eng. 2020;8(3):28-35. doi: 10.11648/j.ijbse.20200803.12

    Copy | Download

  • @article{10.11648/j.ijbse.20200803.12,
      author = {Shimeles Adugna Elemo},
      title = {Toxoplasmosis Sero-Prevalence, Awareness and Risk Behavior Among Pregnant Women Following Antenatal Care in Asella Teaching and Referral Hospital, Asella, Ethiopia},
      journal = {International Journal of Biomedical Science and Engineering},
      volume = {8},
      number = {3},
      pages = {28-35},
      doi = {10.11648/j.ijbse.20200803.12},
      url = {https://doi.org/10.11648/j.ijbse.20200803.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbse.20200803.12},
      abstract = {Toxoplasmosis is caused by infection with the protozoan Toxoplasma gondii (T. gondii), an obligate intracellular parasite. The infections produced a wide range of clinical syndromes in humans, land and sea mammals, and various bird species. Toxoplasmosis is especially important among Immune-compromised patients and pregnant women. Infection of mothers during pregnancy by Toxoplasma gondii may have serious consequences for fetus ranging from miscarriage, central nervous system involvement, retinochoroditis, or at birth subclinical infection. Objectives: To determine Toxoplasmosis sero-prevalence, awareness and risk Behavior among pregnant women following antenatal care in Asella Teaching and Referral Hospital, Asella, Ethiopia. Methods: Institutional based quantitative cross sectional study design was conducted on sample size of 384 from December 1, 2018 to June 30, 2019. To collect the demographic and risk factor related data a pre-tested Structured, questionnaire was used. Serum sample, collected was tested for Toxoplasma Immunoglobulin G (IgG) and Toxoplasma Immunoglobulin M (IgM) antibodies using anti- IgG and IgM antibodies by Toxoplasma immune-chromatographic test (ICT) IgG-IgM test. To show association between the dependent and independent variables a bivariable and multivariable logistic regression model was applied allowing for p, 0.05 and the confidence interval 95%. Result: The overall sero- prevalence of T. gondii in the study area was 81.8%. Three hundred and fourteen (81.8%) of the pregnant women were IgG seropositive, fifty women (13%) were IgM seropositive. fifty women were positive for both IgG and IgM. None of the pregnant women were positive exclusively for IgG and IgM ani-bodies. eating raw meat (COR=3.480, 95%CI: 1.450-8.352; P=0.005, AOR=3.798, 95%CI: 1.249-11.550, P=0.19), using unpasteurized milk (COR=3.860, 95%CI: 2.118-7.037; P=0.000, AOR=3.907, 95% CI: 1.744-8.751, P=0.001), having three or more children (COR=2.194, 95%CI: 1.065-4.518; P=0.033) and Consumption of raw egg (COR=2.042, 95%CI: 1.049-3.974; P=0.036). Conclusion: The sero-prevalence of T. gondii antibodies was high among the pregnant women. Those who consumed raw meat and egg, unpasteurized milk and those who have three or more children were at higher risk of T. gondii infection. Hence, blood screening for Toxoplasmosis, health education and awareness creation among pregnant women should be done during antenatal follow up.},
     year = {2020}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Toxoplasmosis Sero-Prevalence, Awareness and Risk Behavior Among Pregnant Women Following Antenatal Care in Asella Teaching and Referral Hospital, Asella, Ethiopia
    AU  - Shimeles Adugna Elemo
    Y1  - 2020/09/19
    PY  - 2020
    N1  - https://doi.org/10.11648/j.ijbse.20200803.12
    DO  - 10.11648/j.ijbse.20200803.12
    T2  - International Journal of Biomedical Science and Engineering
    JF  - International Journal of Biomedical Science and Engineering
    JO  - International Journal of Biomedical Science and Engineering
    SP  - 28
    EP  - 35
    PB  - Science Publishing Group
    SN  - 2376-7235
    UR  - https://doi.org/10.11648/j.ijbse.20200803.12
    AB  - Toxoplasmosis is caused by infection with the protozoan Toxoplasma gondii (T. gondii), an obligate intracellular parasite. The infections produced a wide range of clinical syndromes in humans, land and sea mammals, and various bird species. Toxoplasmosis is especially important among Immune-compromised patients and pregnant women. Infection of mothers during pregnancy by Toxoplasma gondii may have serious consequences for fetus ranging from miscarriage, central nervous system involvement, retinochoroditis, or at birth subclinical infection. Objectives: To determine Toxoplasmosis sero-prevalence, awareness and risk Behavior among pregnant women following antenatal care in Asella Teaching and Referral Hospital, Asella, Ethiopia. Methods: Institutional based quantitative cross sectional study design was conducted on sample size of 384 from December 1, 2018 to June 30, 2019. To collect the demographic and risk factor related data a pre-tested Structured, questionnaire was used. Serum sample, collected was tested for Toxoplasma Immunoglobulin G (IgG) and Toxoplasma Immunoglobulin M (IgM) antibodies using anti- IgG and IgM antibodies by Toxoplasma immune-chromatographic test (ICT) IgG-IgM test. To show association between the dependent and independent variables a bivariable and multivariable logistic regression model was applied allowing for p, 0.05 and the confidence interval 95%. Result: The overall sero- prevalence of T. gondii in the study area was 81.8%. Three hundred and fourteen (81.8%) of the pregnant women were IgG seropositive, fifty women (13%) were IgM seropositive. fifty women were positive for both IgG and IgM. None of the pregnant women were positive exclusively for IgG and IgM ani-bodies. eating raw meat (COR=3.480, 95%CI: 1.450-8.352; P=0.005, AOR=3.798, 95%CI: 1.249-11.550, P=0.19), using unpasteurized milk (COR=3.860, 95%CI: 2.118-7.037; P=0.000, AOR=3.907, 95% CI: 1.744-8.751, P=0.001), having three or more children (COR=2.194, 95%CI: 1.065-4.518; P=0.033) and Consumption of raw egg (COR=2.042, 95%CI: 1.049-3.974; P=0.036). Conclusion: The sero-prevalence of T. gondii antibodies was high among the pregnant women. Those who consumed raw meat and egg, unpasteurized milk and those who have three or more children were at higher risk of T. gondii infection. Hence, blood screening for Toxoplasmosis, health education and awareness creation among pregnant women should be done during antenatal follow up.
    VL  - 8
    IS  - 3
    ER  - 

    Copy | Download

Author Information
  • Medical Laboratory Sciences Department, Collage of Health Sciences, Arsi University, Asella, Ethiopia

  • Sections