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The Decoction of Cymbopogon citratus (Poaceae) Exerts Anxiolytic and Antioxidant Effects in Mice on Stress Paradigm Tests

Received: 7 February 2023     Accepted: 1 April 2023     Published: 10 May 2023
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Abstract

Cymbopogon citratus has many medicinal properties: it is used to treat headaches, cough, malaria and anxiety or fear in the national dielet. The interest of this study was to evaluate the anxiolytic properties and acute toxicity of the decoction of Cymbopogon citratus leaves. C. citratus was harvested in the Wouri division, Littoral Region of Cameroon. The identification of the families of secondary metabolites was carried out on the leaves decoction through staining and precipitation tests. The animal material consisted of male and female Mus musculus Swiss mice of 18 g at 30 g over aged about 9 weeks and not previously tested. They were used for acute toxicity assessment by administering a single dose of 5000mg/kg. Then, the anxiolytic activity assessment of the decoction on basic and chronic anxiety was done using the following devices: the elevated plus maze, the open field, the hole board and the restrictor. Finally, some oxidative stress parameters such catalase, sulphoxide dismutase, reduced glutathione and malondialdehyde were measured. The extraction yield of the C. citratus decoction was 15.12%. No signs of toxicity were observed at the dose of 5000mg/kg, 14 days after treatment. The Screening of the decoction revealed the presence of saponins, phenolic compounds and sterols. The anxiolytic activity assessment of the decoction on basic and chronic anxiety through the different devices shows that the most effective dose of the C. citratus decoction was 151 mg/kg. In the case of the elevated plus maze test, there was an increase of the number of entries into the open arms, the time spent in the open arms and their respective percentages. However, there was a decrease of the number of entries into the closed arms, the time spent in the closed arms and their percentages. The number of rearing and the mass of stool produced were significantly reduced in the maze, indicating a decrease of the level of anxiety in these mice. The open field and hole board tests also showed that, the C. citratus decoction would have anxiolytic properties. This could be justified may be by the presence of saponins and phenolic compounds. The determination of oxidative stress markers showed that, mice given the decoction and diazepam had elevated catalase, sulphoxide dismutase and reduced glutathione, but their malondialdehyde was low. The results of the study showed that the decoction of Cymbopogon citratus has anxiolytic properties.

Published in International Journal of Biomedical Engineering and Clinical Science (Volume 9, Issue 2)
DOI 10.11648/j.ijbecs.20230902.11
Page(s) 14-23
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

Cymbopogon citratus, Decoction, Acute Toxicity, Anxiolytic

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Cite This Article
  • APA Style

    Jean Pierre Omam Omam, Andre Hamadou, Antoine Kavaye Kandeda, Juliette Koube, Djibrilla Mana Moussa, et al. (2023). The Decoction of Cymbopogon citratus (Poaceae) Exerts Anxiolytic and Antioxidant Effects in Mice on Stress Paradigm Tests. International Journal of Biomedical Engineering and Clinical Science, 9(2), 14-23. https://doi.org/10.11648/j.ijbecs.20230902.11

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    ACS Style

    Jean Pierre Omam Omam; Andre Hamadou; Antoine Kavaye Kandeda; Juliette Koube; Djibrilla Mana Moussa, et al. The Decoction of Cymbopogon citratus (Poaceae) Exerts Anxiolytic and Antioxidant Effects in Mice on Stress Paradigm Tests. Int. J. Biomed. Eng. Clin. Sci. 2023, 9(2), 14-23. doi: 10.11648/j.ijbecs.20230902.11

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    AMA Style

    Jean Pierre Omam Omam, Andre Hamadou, Antoine Kavaye Kandeda, Juliette Koube, Djibrilla Mana Moussa, et al. The Decoction of Cymbopogon citratus (Poaceae) Exerts Anxiolytic and Antioxidant Effects in Mice on Stress Paradigm Tests. Int J Biomed Eng Clin Sci. 2023;9(2):14-23. doi: 10.11648/j.ijbecs.20230902.11

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  • @article{10.11648/j.ijbecs.20230902.11,
      author = {Jean Pierre Omam Omam and Andre Hamadou and Antoine Kavaye Kandeda and Juliette Koube and Djibrilla Mana Moussa and Brice Kevin Tchoupe Djoumsie and Mireille Delphine Minkoulou Ze and Roger Sébastien Ndjock and Elisabeth Ngo Bum},
      title = {The Decoction of Cymbopogon citratus (Poaceae) Exerts Anxiolytic and Antioxidant Effects in Mice on Stress Paradigm Tests},
      journal = {International Journal of Biomedical Engineering and Clinical Science},
      volume = {9},
      number = {2},
      pages = {14-23},
      doi = {10.11648/j.ijbecs.20230902.11},
      url = {https://doi.org/10.11648/j.ijbecs.20230902.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbecs.20230902.11},
      abstract = {Cymbopogon citratus has many medicinal properties: it is used to treat headaches, cough, malaria and anxiety or fear in the national dielet. The interest of this study was to evaluate the anxiolytic properties and acute toxicity of the decoction of Cymbopogon citratus leaves. C. citratus was harvested in the Wouri division, Littoral Region of Cameroon. The identification of the families of secondary metabolites was carried out on the leaves decoction through staining and precipitation tests. The animal material consisted of male and female Mus musculus Swiss mice of 18 g at 30 g over aged about 9 weeks and not previously tested. They were used for acute toxicity assessment by administering a single dose of 5000mg/kg. Then, the anxiolytic activity assessment of the decoction on basic and chronic anxiety was done using the following devices: the elevated plus maze, the open field, the hole board and the restrictor. Finally, some oxidative stress parameters such catalase, sulphoxide dismutase, reduced glutathione and malondialdehyde were measured. The extraction yield of the C. citratus decoction was 15.12%. No signs of toxicity were observed at the dose of 5000mg/kg, 14 days after treatment. The Screening of the decoction revealed the presence of saponins, phenolic compounds and sterols. The anxiolytic activity assessment of the decoction on basic and chronic anxiety through the different devices shows that the most effective dose of the C. citratus decoction was 151 mg/kg. In the case of the elevated plus maze test, there was an increase of the number of entries into the open arms, the time spent in the open arms and their respective percentages. However, there was a decrease of the number of entries into the closed arms, the time spent in the closed arms and their percentages. The number of rearing and the mass of stool produced were significantly reduced in the maze, indicating a decrease of the level of anxiety in these mice. The open field and hole board tests also showed that, the C. citratus decoction would have anxiolytic properties. This could be justified may be by the presence of saponins and phenolic compounds. The determination of oxidative stress markers showed that, mice given the decoction and diazepam had elevated catalase, sulphoxide dismutase and reduced glutathione, but their malondialdehyde was low. The results of the study showed that the decoction of Cymbopogon citratus has anxiolytic properties.},
     year = {2023}
    }
    

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  • TY  - JOUR
    T1  - The Decoction of Cymbopogon citratus (Poaceae) Exerts Anxiolytic and Antioxidant Effects in Mice on Stress Paradigm Tests
    AU  - Jean Pierre Omam Omam
    AU  - Andre Hamadou
    AU  - Antoine Kavaye Kandeda
    AU  - Juliette Koube
    AU  - Djibrilla Mana Moussa
    AU  - Brice Kevin Tchoupe Djoumsie
    AU  - Mireille Delphine Minkoulou Ze
    AU  - Roger Sébastien Ndjock
    AU  - Elisabeth Ngo Bum
    Y1  - 2023/05/10
    PY  - 2023
    N1  - https://doi.org/10.11648/j.ijbecs.20230902.11
    DO  - 10.11648/j.ijbecs.20230902.11
    T2  - International Journal of Biomedical Engineering and Clinical Science
    JF  - International Journal of Biomedical Engineering and Clinical Science
    JO  - International Journal of Biomedical Engineering and Clinical Science
    SP  - 14
    EP  - 23
    PB  - Science Publishing Group
    SN  - 2472-1301
    UR  - https://doi.org/10.11648/j.ijbecs.20230902.11
    AB  - Cymbopogon citratus has many medicinal properties: it is used to treat headaches, cough, malaria and anxiety or fear in the national dielet. The interest of this study was to evaluate the anxiolytic properties and acute toxicity of the decoction of Cymbopogon citratus leaves. C. citratus was harvested in the Wouri division, Littoral Region of Cameroon. The identification of the families of secondary metabolites was carried out on the leaves decoction through staining and precipitation tests. The animal material consisted of male and female Mus musculus Swiss mice of 18 g at 30 g over aged about 9 weeks and not previously tested. They were used for acute toxicity assessment by administering a single dose of 5000mg/kg. Then, the anxiolytic activity assessment of the decoction on basic and chronic anxiety was done using the following devices: the elevated plus maze, the open field, the hole board and the restrictor. Finally, some oxidative stress parameters such catalase, sulphoxide dismutase, reduced glutathione and malondialdehyde were measured. The extraction yield of the C. citratus decoction was 15.12%. No signs of toxicity were observed at the dose of 5000mg/kg, 14 days after treatment. The Screening of the decoction revealed the presence of saponins, phenolic compounds and sterols. The anxiolytic activity assessment of the decoction on basic and chronic anxiety through the different devices shows that the most effective dose of the C. citratus decoction was 151 mg/kg. In the case of the elevated plus maze test, there was an increase of the number of entries into the open arms, the time spent in the open arms and their respective percentages. However, there was a decrease of the number of entries into the closed arms, the time spent in the closed arms and their percentages. The number of rearing and the mass of stool produced were significantly reduced in the maze, indicating a decrease of the level of anxiety in these mice. The open field and hole board tests also showed that, the C. citratus decoction would have anxiolytic properties. This could be justified may be by the presence of saponins and phenolic compounds. The determination of oxidative stress markers showed that, mice given the decoction and diazepam had elevated catalase, sulphoxide dismutase and reduced glutathione, but their malondialdehyde was low. The results of the study showed that the decoction of Cymbopogon citratus has anxiolytic properties.
    VL  - 9
    IS  - 2
    ER  - 

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Author Information
  • Department of Biological Sciences, High Teacher Training College, University of Yaounde I, Yaounde, Cameroon

  • National Institute of Youth and Sport, Yaounde, Cameroon

  • Department of Animal Biology and Physiology, Faculty of Sciences, University of Yaounde I, Yaounde, Cameroon

  • Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Douala, Cameroon

  • Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Douala, Cameroon

  • Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Douala, Cameroon

  • Department of Animal Biology and Physiology, Faculty of Sciences, University of Yaounde I, Yaounde, Cameroon

  • Department of Biological Sciences, Faculty of Sciences, University of Ngaoundere, Ngaoundere, Cameroon

  • Department of Biological Sciences, Faculty of Sciences, University of Ngaoundere, Ngaoundere, Cameroon

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