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Antiproliferative Effects of Ferula assa-foetida’s Extract on PC12 and MCF7 Cancer Cells

Received: 3 August 2020     Accepted: 17 August 2020     Published: 30 October 2020
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Abstract

Background: Ferula assa-foetida is a herbaceous plant of the Umbelliferae family having a broad spectrum of biological activities such as antiseptic, antibacterial, anti-inflammation, and, anti-tumor activity against a wide range of cancers. Among these features, antitumor activity has become more important in recent years and it still demands more investigations to address the underlying mechanisms. Purpose: This current study was conducted to evaluate the anti-proliferative effect of Ferula assa-foetida on PC12 and MCF7 cells as well as examining its mechanisms. Materials and Methods: Cells were cultured in DMEM medium with 10% fetal bovine serum, 100 units/ml penicillin, and 100 µg/ml streptomycin. Cells incubated with different concentrations of the ethanolic extract of Ferula assa-foetida. Notably, cytotoxicity and apoptosis assay were measured by MTT and PI staining, respectively. Results: The MTT results showed that the ethanolic extract of Ferula assa-foetida in concentrations of 10, 7, 5, and 2.5 μM on both PC12 and MCF7 cells had a significant effect in cell viability and apoptosis induction in comparison to control group. Conclusion: In this study, it was determined that Ferula assa-fetida through the induction of apoptosis prevented the growth of PC12 and MCF7 cells and made a reduction in cell viability with different concentrations in a time- and dose-dependent manner. However, more studies are needed to reveal the mechanisms of Ferula assa-foetida’s extract in apoptosis induction.

Published in International Journal of Biomedical Engineering and Clinical Science (Volume 6, Issue 3)
DOI 10.11648/j.ijbecs.20200603.12
Page(s) 60-67
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Ferula assa-foetida, Apoptosis, Cytotoxicity, Cancer, MCF7, PC12

References
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Cite This Article
  • APA Style

    Mina Abroudi, Amir Ganji Fard, Ghazaleh Dadashizadeh, Omid Gholami, Davood Mahdian. (2020). Antiproliferative Effects of Ferula assa-foetida’s Extract on PC12 and MCF7 Cancer Cells. International Journal of Biomedical Engineering and Clinical Science, 6(3), 60-67. https://doi.org/10.11648/j.ijbecs.20200603.12

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    ACS Style

    Mina Abroudi; Amir Ganji Fard; Ghazaleh Dadashizadeh; Omid Gholami; Davood Mahdian. Antiproliferative Effects of Ferula assa-foetida’s Extract on PC12 and MCF7 Cancer Cells. Int. J. Biomed. Eng. Clin. Sci. 2020, 6(3), 60-67. doi: 10.11648/j.ijbecs.20200603.12

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    AMA Style

    Mina Abroudi, Amir Ganji Fard, Ghazaleh Dadashizadeh, Omid Gholami, Davood Mahdian. Antiproliferative Effects of Ferula assa-foetida’s Extract on PC12 and MCF7 Cancer Cells. Int J Biomed Eng Clin Sci. 2020;6(3):60-67. doi: 10.11648/j.ijbecs.20200603.12

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  • @article{10.11648/j.ijbecs.20200603.12,
      author = {Mina Abroudi and Amir Ganji Fard and Ghazaleh Dadashizadeh and Omid Gholami and Davood Mahdian},
      title = {Antiproliferative Effects of Ferula assa-foetida’s Extract on PC12 and MCF7 Cancer Cells},
      journal = {International Journal of Biomedical Engineering and Clinical Science},
      volume = {6},
      number = {3},
      pages = {60-67},
      doi = {10.11648/j.ijbecs.20200603.12},
      url = {https://doi.org/10.11648/j.ijbecs.20200603.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbecs.20200603.12},
      abstract = {Background: Ferula assa-foetida is a herbaceous plant of the Umbelliferae family having a broad spectrum of biological activities such as antiseptic, antibacterial, anti-inflammation, and, anti-tumor activity against a wide range of cancers. Among these features, antitumor activity has become more important in recent years and it still demands more investigations to address the underlying mechanisms. Purpose: This current study was conducted to evaluate the anti-proliferative effect of Ferula assa-foetida on PC12 and MCF7 cells as well as examining its mechanisms. Materials and Methods: Cells were cultured in DMEM medium with 10% fetal bovine serum, 100 units/ml penicillin, and 100 µg/ml streptomycin. Cells incubated with different concentrations of the ethanolic extract of Ferula assa-foetida. Notably, cytotoxicity and apoptosis assay were measured by MTT and PI staining, respectively. Results: The MTT results showed that the ethanolic extract of Ferula assa-foetida in concentrations of 10, 7, 5, and 2.5 μM on both PC12 and MCF7 cells had a significant effect in cell viability and apoptosis induction in comparison to control group. Conclusion: In this study, it was determined that Ferula assa-fetida through the induction of apoptosis prevented the growth of PC12 and MCF7 cells and made a reduction in cell viability with different concentrations in a time- and dose-dependent manner. However, more studies are needed to reveal the mechanisms of Ferula assa-foetida’s extract in apoptosis induction.},
     year = {2020}
    }
    

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  • TY  - JOUR
    T1  - Antiproliferative Effects of Ferula assa-foetida’s Extract on PC12 and MCF7 Cancer Cells
    AU  - Mina Abroudi
    AU  - Amir Ganji Fard
    AU  - Ghazaleh Dadashizadeh
    AU  - Omid Gholami
    AU  - Davood Mahdian
    Y1  - 2020/10/30
    PY  - 2020
    N1  - https://doi.org/10.11648/j.ijbecs.20200603.12
    DO  - 10.11648/j.ijbecs.20200603.12
    T2  - International Journal of Biomedical Engineering and Clinical Science
    JF  - International Journal of Biomedical Engineering and Clinical Science
    JO  - International Journal of Biomedical Engineering and Clinical Science
    SP  - 60
    EP  - 67
    PB  - Science Publishing Group
    SN  - 2472-1301
    UR  - https://doi.org/10.11648/j.ijbecs.20200603.12
    AB  - Background: Ferula assa-foetida is a herbaceous plant of the Umbelliferae family having a broad spectrum of biological activities such as antiseptic, antibacterial, anti-inflammation, and, anti-tumor activity against a wide range of cancers. Among these features, antitumor activity has become more important in recent years and it still demands more investigations to address the underlying mechanisms. Purpose: This current study was conducted to evaluate the anti-proliferative effect of Ferula assa-foetida on PC12 and MCF7 cells as well as examining its mechanisms. Materials and Methods: Cells were cultured in DMEM medium with 10% fetal bovine serum, 100 units/ml penicillin, and 100 µg/ml streptomycin. Cells incubated with different concentrations of the ethanolic extract of Ferula assa-foetida. Notably, cytotoxicity and apoptosis assay were measured by MTT and PI staining, respectively. Results: The MTT results showed that the ethanolic extract of Ferula assa-foetida in concentrations of 10, 7, 5, and 2.5 μM on both PC12 and MCF7 cells had a significant effect in cell viability and apoptosis induction in comparison to control group. Conclusion: In this study, it was determined that Ferula assa-fetida through the induction of apoptosis prevented the growth of PC12 and MCF7 cells and made a reduction in cell viability with different concentrations in a time- and dose-dependent manner. However, more studies are needed to reveal the mechanisms of Ferula assa-foetida’s extract in apoptosis induction.
    VL  - 6
    IS  - 3
    ER  - 

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Author Information
  • Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran

  • Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran

  • Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran

  • Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran

  • Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran

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