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Ampicillin Resistance in Haemophilus influenzae Isolated from Acute Respiratory Infections in Pediatrics

Received: 20 February 2019     Accepted: 10 April 2019     Published: 16 October 2019
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Abstract

Haemophilus influenzae is a bacterium that can cause severe infections, occurring mostly in infants and children younger than five years of age. Antibiotic treatment may cause the emergence of resistant H. influenzae strains, particularly ampicillin-resistant strains. Antimicrobial resistance is a public health threat worldwide, particularly in the developing world. H. influenzae strains have been isolated from broncho-alveolar lavages (BALs), nasopharyngeal swabs, and otitis media from children in two paediatric centers at Dakar, Senegal. Antibiotic susceptibility testing was carried out using strips E Test ®t method that provides the ability to precisely determine the minimum inhibitory concentration (MIC). A total of 16 H. influenzae strains have been isolated and identified, including 16.7% of ampicillin-resistant patterns (all β-lactamase-negative), 9.4% of the isolates were resistant to cefaclor (MIC90 = 16 µg/ml) while 100% were susceptible to cefixime to (MIC90 = 0.38 µg/ml). Interestingly, fluoroquinolones were fully active with very low MIC90. Macrolide were still active against H. influenzae isoles although with higher MIC azitrhomycin MIC90= 3µg/ml, clarithromycin MIC90= 12µg/ml. Ampicillin-resistance has become increasingly reported in H. influenzae, suggesting a continuous laboratory based surveillance for antimicrobial resistance pattern for a better management of acute respiratory infections, particularly in low incomes settings.

Published in International Journal of Biomedical Engineering and Clinical Science (Volume 5, Issue 3)
DOI 10.11648/j.ijbecs.20190503.12
Page(s) 40-44
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Respiratory Tract Infections, Haemophilus influenzae, Ampicillin Resistance

References
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Cite This Article
  • APA Style

    Abdoulaye Diop, Assane Dieng, Abdoulaye Seck, Amary Fall, Amadou Diop, et al. (2019). Ampicillin Resistance in Haemophilus influenzae Isolated from Acute Respiratory Infections in Pediatrics. International Journal of Biomedical Engineering and Clinical Science, 5(3), 40-44. https://doi.org/10.11648/j.ijbecs.20190503.12

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    ACS Style

    Abdoulaye Diop; Assane Dieng; Abdoulaye Seck; Amary Fall; Amadou Diop, et al. Ampicillin Resistance in Haemophilus influenzae Isolated from Acute Respiratory Infections in Pediatrics. Int. J. Biomed. Eng. Clin. Sci. 2019, 5(3), 40-44. doi: 10.11648/j.ijbecs.20190503.12

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    AMA Style

    Abdoulaye Diop, Assane Dieng, Abdoulaye Seck, Amary Fall, Amadou Diop, et al. Ampicillin Resistance in Haemophilus influenzae Isolated from Acute Respiratory Infections in Pediatrics. Int J Biomed Eng Clin Sci. 2019;5(3):40-44. doi: 10.11648/j.ijbecs.20190503.12

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  • @article{10.11648/j.ijbecs.20190503.12,
      author = {Abdoulaye Diop and Assane Dieng and Abdoulaye Seck and Amary Fall and Amadou Diop and Djibril Boiro and Jean Baptisse Niokhor Diouf and Modou Gueye and Mbayame Niang and Makhtar Camara and Cheikh Saad Bouh Boye},
      title = {Ampicillin Resistance in Haemophilus influenzae Isolated from Acute Respiratory Infections in Pediatrics},
      journal = {International Journal of Biomedical Engineering and Clinical Science},
      volume = {5},
      number = {3},
      pages = {40-44},
      doi = {10.11648/j.ijbecs.20190503.12},
      url = {https://doi.org/10.11648/j.ijbecs.20190503.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbecs.20190503.12},
      abstract = {Haemophilus influenzae is a bacterium that can cause severe infections, occurring mostly in infants and children younger than five years of age. Antibiotic treatment may cause the emergence of resistant H. influenzae strains, particularly ampicillin-resistant strains. Antimicrobial resistance is a public health threat worldwide, particularly in the developing world. H. influenzae strains have been isolated from broncho-alveolar lavages (BALs), nasopharyngeal swabs, and otitis media from children in two paediatric centers at Dakar, Senegal. Antibiotic susceptibility testing was carried out using strips E Test ®t method that provides the ability to precisely determine the minimum inhibitory concentration (MIC). A total of 16 H. influenzae strains have been isolated and identified, including 16.7% of ampicillin-resistant patterns (all β-lactamase-negative), 9.4% of the isolates were resistant to cefaclor (MIC90 = 16 µg/ml) while 100% were susceptible to cefixime to (MIC90 = 0.38 µg/ml). Interestingly, fluoroquinolones were fully active with very low MIC90. Macrolide were still active against H. influenzae isoles although with higher MIC azitrhomycin MIC90= 3µg/ml, clarithromycin MIC90= 12µg/ml. Ampicillin-resistance has become increasingly reported in H. influenzae, suggesting a continuous laboratory based surveillance for antimicrobial resistance pattern for a better management of acute respiratory infections, particularly in low incomes settings.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Ampicillin Resistance in Haemophilus influenzae Isolated from Acute Respiratory Infections in Pediatrics
    AU  - Abdoulaye Diop
    AU  - Assane Dieng
    AU  - Abdoulaye Seck
    AU  - Amary Fall
    AU  - Amadou Diop
    AU  - Djibril Boiro
    AU  - Jean Baptisse Niokhor Diouf
    AU  - Modou Gueye
    AU  - Mbayame Niang
    AU  - Makhtar Camara
    AU  - Cheikh Saad Bouh Boye
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    DO  - 10.11648/j.ijbecs.20190503.12
    T2  - International Journal of Biomedical Engineering and Clinical Science
    JF  - International Journal of Biomedical Engineering and Clinical Science
    JO  - International Journal of Biomedical Engineering and Clinical Science
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    EP  - 44
    PB  - Science Publishing Group
    SN  - 2472-1301
    UR  - https://doi.org/10.11648/j.ijbecs.20190503.12
    AB  - Haemophilus influenzae is a bacterium that can cause severe infections, occurring mostly in infants and children younger than five years of age. Antibiotic treatment may cause the emergence of resistant H. influenzae strains, particularly ampicillin-resistant strains. Antimicrobial resistance is a public health threat worldwide, particularly in the developing world. H. influenzae strains have been isolated from broncho-alveolar lavages (BALs), nasopharyngeal swabs, and otitis media from children in two paediatric centers at Dakar, Senegal. Antibiotic susceptibility testing was carried out using strips E Test ®t method that provides the ability to precisely determine the minimum inhibitory concentration (MIC). A total of 16 H. influenzae strains have been isolated and identified, including 16.7% of ampicillin-resistant patterns (all β-lactamase-negative), 9.4% of the isolates were resistant to cefaclor (MIC90 = 16 µg/ml) while 100% were susceptible to cefixime to (MIC90 = 0.38 µg/ml). Interestingly, fluoroquinolones were fully active with very low MIC90. Macrolide were still active against H. influenzae isoles although with higher MIC azitrhomycin MIC90= 3µg/ml, clarithromycin MIC90= 12µg/ml. Ampicillin-resistance has become increasingly reported in H. influenzae, suggesting a continuous laboratory based surveillance for antimicrobial resistance pattern for a better management of acute respiratory infections, particularly in low incomes settings.
    VL  - 5
    IS  - 3
    ER  - 

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Author Information
  • Bacteriology and Virology Laboratory, Le Dantec Teaching Hospital, Dakar, Senegal

  • Bacteriology and Virology Laboratory, Le Dantec Teaching Hospital, Dakar, Senegal

  • Medical Virology Unit, Institute Pasteur, Dakar, Senegal

  • Medical Virology Unit, Institute Pasteur, Dakar, Senegal

  • Bacteriology and Virology Laboratory, Le Dantec Teaching Hospital, Dakar, Senegal

  • Paediatric Unit, Abass NDAO Teaching Hospital, Dakar, Senegal

  • Paediatric Unit, Roi Baudouin Hospital, Dakar, Senegal

  • Paediatric Unit, Abass NDAO Teaching Hospital, Dakar, Senegal

  • Medical Virology Unit, Institute Pasteur, Dakar, Senegal

  • Bacteriology and Virology Laboratory, Le Dantec Teaching Hospital, Dakar, Senegal

  • Bacteriology and Virology Laboratory, Le Dantec Teaching Hospital, Dakar, Senegal

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