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In vitro Evaluation of the Anti-scavenging and Anthelmintic Activities of Artocarpus heterophyllus LAM Leaves (Moraceae) in the Democratic Republic of Congo

Received: 17 August 2019     Accepted: 6 September 2019     Published: 24 September 2019
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Abstract

The extracts of Arthocarpus heterophyllus Lam. leaves were evaluated in vitro for their anthelmintic activity. Benhamia rosea and B. itoleisis were used as animal models and Albendazole as reference product (positive control). After calculating the yield, it appears that the ethanol extracts had given a better yield (0.70%) compared to the organic extracts. The result of the phytochemical screening by TLC (thin layer chromatography) showed the presence of phenolic compounds including anthocyanins, coumarins, anthraquinones, phenol acids and terpenoids. From this study, it appears that A. heterophyllus Lam. contains various secondary metabolites such as flavonoids (2.63±0.007mg EQ/100g MS), phenolic acids, coumarins, anthraquinones, terpenoids and anthocyanins (10.46±1.05 mg/100 MS) and total polyphenols (27.33±9.34 mg EAG/100 g MS). The organic/terpenic acids extract showed very high antioxidant activity against the ABTS radical (IC50: 0.97 ± 0.13 µg/ml). The ethanolic and organic acid extracts from the leaves of this plant species have an anthelminthic activity, but this activity is dose dependent. However, at the lowest concentration (0.625 mg/mL), ethanolic extract showed better activity with a paralysis time of 67.3±1.8 minutes compared to 76±2.1 minutes for the organic extract. But the mortality rate at the lowest concentration was higher for organic extracts, at 62.7% compared to 33.3% for ethanol extracts. It is therefore desirable to test bioactive extracts on gastrointestinal parasites of farm animals in order to confirm the results obtained. Ongoing in-depth phytochemical studies will identify the chemical compound (s) and active principle (s) for the formulation of anthelminthic phytomedicine for managing pathologies due to helminthes in farm animals.

Published in International Journal of Biomedical Engineering and Clinical Science (Volume 5, Issue 2)
DOI 10.11648/j.ijbecs.20190502.11
Page(s) 7-15
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Anthelminthic Activity, Earthworm, Phytomedicine, Arthocarpus heterophyllus

References
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Cite This Article
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    Lengbiye Moke Emmanuel, Koto-te-Nyiwa Ngbolua, Lin Marcelin Messi, Mbembo wa Mbembo Blaise, Gédéon Ngiala Bongo, et al. (2019). In vitro Evaluation of the Anti-scavenging and Anthelmintic Activities of Artocarpus heterophyllus LAM Leaves (Moraceae) in the Democratic Republic of Congo. International Journal of Biomedical Engineering and Clinical Science, 5(2), 7-15. https://doi.org/10.11648/j.ijbecs.20190502.11

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    ACS Style

    Lengbiye Moke Emmanuel; Koto-te-Nyiwa Ngbolua; Lin Marcelin Messi; Mbembo wa Mbembo Blaise; Gédéon Ngiala Bongo, et al. In vitro Evaluation of the Anti-scavenging and Anthelmintic Activities of Artocarpus heterophyllus LAM Leaves (Moraceae) in the Democratic Republic of Congo. Int. J. Biomed. Eng. Clin. Sci. 2019, 5(2), 7-15. doi: 10.11648/j.ijbecs.20190502.11

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    AMA Style

    Lengbiye Moke Emmanuel, Koto-te-Nyiwa Ngbolua, Lin Marcelin Messi, Mbembo wa Mbembo Blaise, Gédéon Ngiala Bongo, et al. In vitro Evaluation of the Anti-scavenging and Anthelmintic Activities of Artocarpus heterophyllus LAM Leaves (Moraceae) in the Democratic Republic of Congo. Int J Biomed Eng Clin Sci. 2019;5(2):7-15. doi: 10.11648/j.ijbecs.20190502.11

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  • @article{10.11648/j.ijbecs.20190502.11,
      author = {Lengbiye Moke Emmanuel and Koto-te-Nyiwa Ngbolua and Lin Marcelin Messi and Mbembo wa Mbembo Blaise and Gédéon Ngiala Bongo and Mutwale Kapepula Paulin and Ngombe Kabamba Nadège and Joséphine Ngo Mbing and Dieudonné Emmanuel Pegnyemb and Pius Tshimankinda Mpiana},
      title = {In vitro Evaluation of the Anti-scavenging and Anthelmintic Activities of Artocarpus heterophyllus LAM Leaves (Moraceae) in the Democratic Republic of Congo},
      journal = {International Journal of Biomedical Engineering and Clinical Science},
      volume = {5},
      number = {2},
      pages = {7-15},
      doi = {10.11648/j.ijbecs.20190502.11},
      url = {https://doi.org/10.11648/j.ijbecs.20190502.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbecs.20190502.11},
      abstract = {The extracts of Arthocarpus heterophyllus Lam. leaves were evaluated in vitro for their anthelmintic activity. Benhamia rosea and B. itoleisis were used as animal models and Albendazole as reference product (positive control). After calculating the yield, it appears that the ethanol extracts had given a better yield (0.70%) compared to the organic extracts. The result of the phytochemical screening by TLC (thin layer chromatography) showed the presence of phenolic compounds including anthocyanins, coumarins, anthraquinones, phenol acids and terpenoids. From this study, it appears that A. heterophyllus Lam. contains various secondary metabolites such as flavonoids (2.63±0.007mg EQ/100g MS), phenolic acids, coumarins, anthraquinones, terpenoids and anthocyanins (10.46±1.05 mg/100 MS) and total polyphenols (27.33±9.34 mg EAG/100 g MS). The organic/terpenic acids extract showed very high antioxidant activity against the ABTS radical (IC50: 0.97 ± 0.13 µg/ml). The ethanolic and organic acid extracts from the leaves of this plant species have an anthelminthic activity, but this activity is dose dependent. However, at the lowest concentration (0.625 mg/mL), ethanolic extract showed better activity with a paralysis time of 67.3±1.8 minutes compared to 76±2.1 minutes for the organic extract. But the mortality rate at the lowest concentration was higher for organic extracts, at 62.7% compared to 33.3% for ethanol extracts. It is therefore desirable to test bioactive extracts on gastrointestinal parasites of farm animals in order to confirm the results obtained. Ongoing in-depth phytochemical studies will identify the chemical compound (s) and active principle (s) for the formulation of anthelminthic phytomedicine for managing pathologies due to helminthes in farm animals.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - In vitro Evaluation of the Anti-scavenging and Anthelmintic Activities of Artocarpus heterophyllus LAM Leaves (Moraceae) in the Democratic Republic of Congo
    AU  - Lengbiye Moke Emmanuel
    AU  - Koto-te-Nyiwa Ngbolua
    AU  - Lin Marcelin Messi
    AU  - Mbembo wa Mbembo Blaise
    AU  - Gédéon Ngiala Bongo
    AU  - Mutwale Kapepula Paulin
    AU  - Ngombe Kabamba Nadège
    AU  - Joséphine Ngo Mbing
    AU  - Dieudonné Emmanuel Pegnyemb
    AU  - Pius Tshimankinda Mpiana
    Y1  - 2019/09/24
    PY  - 2019
    N1  - https://doi.org/10.11648/j.ijbecs.20190502.11
    DO  - 10.11648/j.ijbecs.20190502.11
    T2  - International Journal of Biomedical Engineering and Clinical Science
    JF  - International Journal of Biomedical Engineering and Clinical Science
    JO  - International Journal of Biomedical Engineering and Clinical Science
    SP  - 7
    EP  - 15
    PB  - Science Publishing Group
    SN  - 2472-1301
    UR  - https://doi.org/10.11648/j.ijbecs.20190502.11
    AB  - The extracts of Arthocarpus heterophyllus Lam. leaves were evaluated in vitro for their anthelmintic activity. Benhamia rosea and B. itoleisis were used as animal models and Albendazole as reference product (positive control). After calculating the yield, it appears that the ethanol extracts had given a better yield (0.70%) compared to the organic extracts. The result of the phytochemical screening by TLC (thin layer chromatography) showed the presence of phenolic compounds including anthocyanins, coumarins, anthraquinones, phenol acids and terpenoids. From this study, it appears that A. heterophyllus Lam. contains various secondary metabolites such as flavonoids (2.63±0.007mg EQ/100g MS), phenolic acids, coumarins, anthraquinones, terpenoids and anthocyanins (10.46±1.05 mg/100 MS) and total polyphenols (27.33±9.34 mg EAG/100 g MS). The organic/terpenic acids extract showed very high antioxidant activity against the ABTS radical (IC50: 0.97 ± 0.13 µg/ml). The ethanolic and organic acid extracts from the leaves of this plant species have an anthelminthic activity, but this activity is dose dependent. However, at the lowest concentration (0.625 mg/mL), ethanolic extract showed better activity with a paralysis time of 67.3±1.8 minutes compared to 76±2.1 minutes for the organic extract. But the mortality rate at the lowest concentration was higher for organic extracts, at 62.7% compared to 33.3% for ethanol extracts. It is therefore desirable to test bioactive extracts on gastrointestinal parasites of farm animals in order to confirm the results obtained. Ongoing in-depth phytochemical studies will identify the chemical compound (s) and active principle (s) for the formulation of anthelminthic phytomedicine for managing pathologies due to helminthes in farm animals.
    VL  - 5
    IS  - 2
    ER  - 

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Author Information
  • Department of Biology, University of Kinshasa, Kinshasa, Democratic Republic of the Congo

  • Department of Biology, University of Kinshasa, Kinshasa, Democratic Republic of the Congo

  • Department of Organic Chemistry, University of Yaounde I, Yaounde, Cameroun

  • Department of Biology, University of Kinshasa, Kinshasa, Democratic Republic of the Congo

  • Department of Biology, University of Kinshasa, Kinshasa, Democratic Republic of the Congo

  • Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of the Congo

  • Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of the Congo

  • Department of Organic Chemistry, University of Yaounde I, Yaounde, Cameroun

  • Department of Organic Chemistry, University of Yaounde I, Yaounde, Cameroun

  • Department of Chemistry, University of Kinshasa, Kinshasa, Democratic Republic of the Congo

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