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Compare the Chlorophyll Amount in Three Brown Algae Species of the Persian Gulf by Using Three Solvents and Applying Two Formulas

Received: 14 October 2017     Accepted: 26 October 2017     Published: 22 November 2017
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Abstract

Chlorophyll is the green pigment in plants that helps to absorb sunlight and converts it into energy. It is believed that it is beneficial for the human body. Therefore in this study, the chlorophyll amount in three brown algae species (Sirophysalis trinodis, Polycladia myrica and Colpomenia sinuosa) of the Persian Gulf by using three solvents and applying two formulas was compared. Acetone, diethyl ether and methanol were used as solvents. Results showed that the chlorophyll amount in different species dependent on solvent type and cell wall structure of algae. The highest chlorophyll a and x+c contents were observed in S. trinodis species. Also, the highest content of chlorophyll c1+c2 was showed in C. sinuosa by the extraction with diethyl ether solvent. In both formulas was no significant difference between the same solvents in the amount of chlorophyll a. In general, the aim of this study due to the diversity and dispersion of algae and also their potential use in the food industry, cosmetics and pharmaceutical goods was to choose a suitable solvent for better extraction of chlorophyll as an active ingredient. Hence, in this study, the acetone was a better solvent.

Published in International Journal of Biochemistry, Biophysics & Molecular Biology (Volume 2, Issue 6)
DOI 10.11648/j.ijbbmb.20170206.14
Page(s) 77-79
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Chlorophyll, Brown Algae, Solvent, Formula, Persian Gulf

References
[1] Christaki E, Bonos E, Giannenas I, Florou-Paneri P (2013) Functional properties of carotenoids originating from algae. J Sci Food Agri 93: 5–11.
[2] Dere S, Gunes T, Sivaci R (1998) Spectrophotometric Determination of Chlorophyll - A, B and Total Carotenoid Contents of Some Algae Species Using Different Solvents. Turk J Botany 22: 13-17.
[3] Hsu CY, Chao PY, Hu SP, Yang CM (2013) The antioxidant and free radical scavenging activities of chlorophylls and pheophytins. Food Nut Sci 4: 1-8.
[4] Huang TL, Cong HB (2007) A New Method for Determination of Chlorophylls in Freshwater Algae. Environ Monit Assess 129: 1–7.
[5] Inanc AL (2011) Chlorophyll: Structural Properties, Health Benefits and Its Occurrence in Virgin Olive Oils. Akademik Gıda 9: 26-32.
[6] Jeffrey SW, Humphrey GF (1975) New spectrophotometric equations for determining chlorophylls a, b, C1 and C2 in higher plants, algae and natural phytoplankton. Biochem physiol Pflanzen 167: 191-194.
[7] Lenore SC, Amold EG, Aadrew DE (2001) Standard Methods for the Examination of Water and Wastewater. American Public Health Association, American Water Works Association, Water Environment Federation. p. 235–255.
[8] Lee RE (2008) Phycology. 4th ed. Cambridge, England, Cambridge University press.
[9] Sakthivel R, Devi KP (2015) Evaluation of physicochemical properties, proximate and nutritional composition of Gracilaria edulis collected from Palk Bay. J Food Chem 174: 68–74.
[10] Sartoryl DP, Grobbelaas JU (1984) Extraction of chlorophyll a from freshwater phytoplankton for spectrophotometric analysis. Hydrobiologia 114: 177-187.
[11] Wellburn AR (1994) The Spectral Determination of Chlorophylls a and b, as well as Total Carotenoids, Using Various Solvents with Spectrophotometers of Different Resolution. J Plant Physiol 144: 307-31.
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    Yasaman Etemadian, Bahareh Shabanpour, Vida Ghaemi, Moazameh Kordjazi. (2017). Compare the Chlorophyll Amount in Three Brown Algae Species of the Persian Gulf by Using Three Solvents and Applying Two Formulas. International Journal of Biochemistry, Biophysics & Molecular Biology, 2(6), 77-79. https://doi.org/10.11648/j.ijbbmb.20170206.14

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    ACS Style

    Yasaman Etemadian; Bahareh Shabanpour; Vida Ghaemi; Moazameh Kordjazi. Compare the Chlorophyll Amount in Three Brown Algae Species of the Persian Gulf by Using Three Solvents and Applying Two Formulas. Int. J. Biochem. Biophys. Mol. Biol. 2017, 2(6), 77-79. doi: 10.11648/j.ijbbmb.20170206.14

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    AMA Style

    Yasaman Etemadian, Bahareh Shabanpour, Vida Ghaemi, Moazameh Kordjazi. Compare the Chlorophyll Amount in Three Brown Algae Species of the Persian Gulf by Using Three Solvents and Applying Two Formulas. Int J Biochem Biophys Mol Biol. 2017;2(6):77-79. doi: 10.11648/j.ijbbmb.20170206.14

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  • @article{10.11648/j.ijbbmb.20170206.14,
      author = {Yasaman Etemadian and Bahareh Shabanpour and Vida Ghaemi and Moazameh Kordjazi},
      title = {Compare the Chlorophyll Amount in Three Brown Algae Species of the Persian Gulf by Using Three Solvents and Applying Two Formulas},
      journal = {International Journal of Biochemistry, Biophysics & Molecular Biology},
      volume = {2},
      number = {6},
      pages = {77-79},
      doi = {10.11648/j.ijbbmb.20170206.14},
      url = {https://doi.org/10.11648/j.ijbbmb.20170206.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbbmb.20170206.14},
      abstract = {Chlorophyll is the green pigment in plants that helps to absorb sunlight and converts it into energy. It is believed that it is beneficial for the human body. Therefore in this study, the chlorophyll amount in three brown algae species (Sirophysalis trinodis, Polycladia myrica and Colpomenia sinuosa) of the Persian Gulf by using three solvents and applying two formulas was compared. Acetone, diethyl ether and methanol were used as solvents. Results showed that the chlorophyll amount in different species dependent on solvent type and cell wall structure of algae. The highest chlorophyll a and x+c contents were observed in S. trinodis species. Also, the highest content of chlorophyll c1+c2 was showed in C. sinuosa by the extraction with diethyl ether solvent. In both formulas was no significant difference between the same solvents in the amount of chlorophyll a. In general, the aim of this study due to the diversity and dispersion of algae and also their potential use in the food industry, cosmetics and pharmaceutical goods was to choose a suitable solvent for better extraction of chlorophyll as an active ingredient. Hence, in this study, the acetone was a better solvent.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Compare the Chlorophyll Amount in Three Brown Algae Species of the Persian Gulf by Using Three Solvents and Applying Two Formulas
    AU  - Yasaman Etemadian
    AU  - Bahareh Shabanpour
    AU  - Vida Ghaemi
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    DO  - 10.11648/j.ijbbmb.20170206.14
    T2  - International Journal of Biochemistry, Biophysics & Molecular Biology
    JF  - International Journal of Biochemistry, Biophysics & Molecular Biology
    JO  - International Journal of Biochemistry, Biophysics & Molecular Biology
    SP  - 77
    EP  - 79
    PB  - Science Publishing Group
    SN  - 2575-5862
    UR  - https://doi.org/10.11648/j.ijbbmb.20170206.14
    AB  - Chlorophyll is the green pigment in plants that helps to absorb sunlight and converts it into energy. It is believed that it is beneficial for the human body. Therefore in this study, the chlorophyll amount in three brown algae species (Sirophysalis trinodis, Polycladia myrica and Colpomenia sinuosa) of the Persian Gulf by using three solvents and applying two formulas was compared. Acetone, diethyl ether and methanol were used as solvents. Results showed that the chlorophyll amount in different species dependent on solvent type and cell wall structure of algae. The highest chlorophyll a and x+c contents were observed in S. trinodis species. Also, the highest content of chlorophyll c1+c2 was showed in C. sinuosa by the extraction with diethyl ether solvent. In both formulas was no significant difference between the same solvents in the amount of chlorophyll a. In general, the aim of this study due to the diversity and dispersion of algae and also their potential use in the food industry, cosmetics and pharmaceutical goods was to choose a suitable solvent for better extraction of chlorophyll as an active ingredient. Hence, in this study, the acetone was a better solvent.
    VL  - 2
    IS  - 6
    ER  - 

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Author Information
  • Faculty of Fisheries Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

  • Faculty of Fisheries Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

  • Department of Fisheries, Faculty of Natural Resources, Zabol University, Zabol, Iran

  • Faculty of Fisheries Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

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