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Molecular Screening of Glucose-6-Phosphate Dehydrogenase Among Deficient Children Aged 0-5 Years with Plasmodium falciparum Malaria in Katsina State, Nigeria

Received: 18 November 2016     Accepted: 7 January 2017     Published: 3 February 2017
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Abstract

G6PD deficiency is one of the most common human enzyme deficiencies in the world. It is particularly common in populations living in malaria-endemic areas. The aim of this study is to molecularly screen G6PD enzyme among deficient children (aged 0-5 years) with Plasmodium falciparum malaria in Katsina State, North-western Nigeria, and a region with no any molecular information on G6PD enzyme. A total of 200 blood samples were collected from children with Plasmodium falciparum malaria attending six selected hospitals located across the three senatorial zones of the state from March 2015 to May 2015. G6PD deficiency was detected qualitatively using G6PD screening test. Polymerase Chain Reaction (PCR) of 7 samples (6 deficient and 1 control) shows the presence of G202A mutation in all the samples. The nucleotides sequence obtained from sequencing reaction of one deficient and one control samples are 97% homologous to other G6PD genes of different strains. This study has indicated a high prevalence of G6PD deficiency among the study population. Based on the results obtained, there is a need for the routine screening of children for G6PD deficiency in our environment and training of paediatricians in order to avoid cases of drug-induced anaemia, particularly in the treatment of malaria.

Published in International Journal of Biochemistry, Biophysics & Molecular Biology (Volume 1, Issue 2)
DOI 10.11648/j.ijbbmb.20160102.13
Page(s) 46-49
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Glucose-6-phosphate Dehydrogenase, Plasmodium falciparum, Malaria, Children, Katsina State, Nigeria

References
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[3] C. Ruwende,S. C. Khoo and R. W. Snow, “Natural selection of hemi- and heterozygotes for G6PD deficiency in Africa by resistance to severe malaria,” Nature, 1995, vol. 376, pp. 246-249.
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[5] T. G. Clark,A. E. Fry and S. Auburn, “Allelic heterogeneity of G6PD deficiency in West Africa and severe malaria susceptibility,” European Journal of Human Genetics, 2009, vol. 17, pp. 1080–5.
[6] E. Beutler,“G6PD Population genetics and clinical manifestation,”Blood Reviews, 1996, vol. 10, pp. 45-52.
[7] L. Luzzatto,“Glucose-6-phosphate dehydrogenase deficiency from genotype to phenotype, Haematologica, 2006, vol. 91, pp. 1303–1306
[8] T. Z. Lui, T. F. Lin, I. J. Hung,J. S. Wei and D. T. Y. Chiu, “Enhanced Susceptibility of Erythrocytes Deficient in Glucose-6-Phosphate Dehydrogenase in Alloxan, GSH-induced decrease in Red Cell Deformability,” Life Science, 1994, vol. 55, pp. l55-160.
[9] S. Udomsak, K. Srivucha, T. Sombat, W. Polsat, C. Kobsiri, Y. M. Hla, M. Pannamas, J. W. Nicholas, R. Victor, M. B. Gary, andL. Sornchai, “Clinical Trial of Oral Artesunate with or without High Dose Primaquine for the Treatment of vivax malaria in Thailand,” American Journal of Tropical Medical Hygiene, 2003, vol. 6, pp. 14-18.
[10] P. J. Mason, J. M. Bautista and F. Gilsanz, G6PD deficiency: “The genotype phenotype association,” Blood Reviews., 2007, vol. 21, pp. 267-83.
[11] M. D. Cappellini and G. Fiorelli, “Glucose-6-phosphate dehydrogenase deficiency,” Lancet, 2008, vol. 371 pp. 64-74.
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[13] D. R. Miller and R. l. Baehner, “Blood Diseases of Infancy and Childhood,” 7thed., Mosby, St., Louis: MO, 1995, pp. 866-923.
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[15] Database resources of the National Center for Biotechnology Information, Oxford University Press on behalf of Nucleic Acids Research 2015.
[16] I. Z. Isaac, A. S. Mainasara, O. Erhabor, S. T. Omojuyigbe, M. K. Dallatu and L. S. Bilbis,“Glucose-6-phosphate dehydrogenase deficiency among children attending the Emergency Paediatric Unit of Usmanu Danfodiyo University Teaching Hospital, Sokoto, Nigeria,” International Journal of General Medicine, 2013, vol.6, pp. 557-562.
[17] E. O. Akanni, B. S. A. Oseni and V. O. Agbona, “Glucose-6-phosphate dehydrogenase deficiency in blood donors and jaundiced neonates in Oshogbo, Nigeria,” Journal of Medical Laboratory Diagnostics, 2010, vol. 1 pp. 1–4.
[18] D. Egesie, D. E. Joseph, I. Isiguzoro and U. G. Egesie, “Glucose-6-phosphate dehydrogenase activity and deficiency in a population of Nigerian male residents in Jos,” Nigerian Journal of Physiological Science, 2008, vol. 1–2, pp. 9–11.
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    Bello Ibrahim, Abdulsalami Mohammed Sani, Bulus Timothy, Mukhtar Gambo Lawal, Abdullahi Mohammed, et al. (2017). Molecular Screening of Glucose-6-Phosphate Dehydrogenase Among Deficient Children Aged 0-5 Years with Plasmodium falciparum Malaria in Katsina State, Nigeria. International Journal of Biochemistry, Biophysics & Molecular Biology, 1(2), 46-49. https://doi.org/10.11648/j.ijbbmb.20160102.13

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    ACS Style

    Bello Ibrahim; Abdulsalami Mohammed Sani; Bulus Timothy; Mukhtar Gambo Lawal; Abdullahi Mohammed, et al. Molecular Screening of Glucose-6-Phosphate Dehydrogenase Among Deficient Children Aged 0-5 Years with Plasmodium falciparum Malaria in Katsina State, Nigeria. Int. J. Biochem. Biophys. Mol. Biol. 2017, 1(2), 46-49. doi: 10.11648/j.ijbbmb.20160102.13

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    AMA Style

    Bello Ibrahim, Abdulsalami Mohammed Sani, Bulus Timothy, Mukhtar Gambo Lawal, Abdullahi Mohammed, et al. Molecular Screening of Glucose-6-Phosphate Dehydrogenase Among Deficient Children Aged 0-5 Years with Plasmodium falciparum Malaria in Katsina State, Nigeria. Int J Biochem Biophys Mol Biol. 2017;1(2):46-49. doi: 10.11648/j.ijbbmb.20160102.13

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  • @article{10.11648/j.ijbbmb.20160102.13,
      author = {Bello Ibrahim and Abdulsalami Mohammed Sani and Bulus Timothy and Mukhtar Gambo Lawal and Abdullahi Mohammed and Abdurrahman Siraj Abubakar and Ahmad Mohammad},
      title = {Molecular Screening of Glucose-6-Phosphate Dehydrogenase Among Deficient Children Aged 0-5 Years with Plasmodium falciparum Malaria in Katsina State, Nigeria},
      journal = {International Journal of Biochemistry, Biophysics & Molecular Biology},
      volume = {1},
      number = {2},
      pages = {46-49},
      doi = {10.11648/j.ijbbmb.20160102.13},
      url = {https://doi.org/10.11648/j.ijbbmb.20160102.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbbmb.20160102.13},
      abstract = {G6PD deficiency is one of the most common human enzyme deficiencies in the world. It is particularly common in populations living in malaria-endemic areas. The aim of this study is to molecularly screen G6PD enzyme among deficient children (aged 0-5 years) with Plasmodium falciparum malaria in Katsina State, North-western Nigeria, and a region with no any molecular information on G6PD enzyme. A total of 200 blood samples were collected from children with Plasmodium falciparum malaria attending six selected hospitals located across the three senatorial zones of the state from March 2015 to May 2015. G6PD deficiency was detected qualitatively using G6PD screening test. Polymerase Chain Reaction (PCR) of 7 samples (6 deficient and 1 control) shows the presence of G202A mutation in all the samples. The nucleotides sequence obtained from sequencing reaction of one deficient and one control samples are 97% homologous to other G6PD genes of different strains. This study has indicated a high prevalence of G6PD deficiency among the study population. Based on the results obtained, there is a need for the routine screening of children for G6PD deficiency in our environment and training of paediatricians in order to avoid cases of drug-induced anaemia, particularly in the treatment of malaria.},
     year = {2017}
    }
    

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    T1  - Molecular Screening of Glucose-6-Phosphate Dehydrogenase Among Deficient Children Aged 0-5 Years with Plasmodium falciparum Malaria in Katsina State, Nigeria
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    AB  - G6PD deficiency is one of the most common human enzyme deficiencies in the world. It is particularly common in populations living in malaria-endemic areas. The aim of this study is to molecularly screen G6PD enzyme among deficient children (aged 0-5 years) with Plasmodium falciparum malaria in Katsina State, North-western Nigeria, and a region with no any molecular information on G6PD enzyme. A total of 200 blood samples were collected from children with Plasmodium falciparum malaria attending six selected hospitals located across the three senatorial zones of the state from March 2015 to May 2015. G6PD deficiency was detected qualitatively using G6PD screening test. Polymerase Chain Reaction (PCR) of 7 samples (6 deficient and 1 control) shows the presence of G202A mutation in all the samples. The nucleotides sequence obtained from sequencing reaction of one deficient and one control samples are 97% homologous to other G6PD genes of different strains. This study has indicated a high prevalence of G6PD deficiency among the study population. Based on the results obtained, there is a need for the routine screening of children for G6PD deficiency in our environment and training of paediatricians in order to avoid cases of drug-induced anaemia, particularly in the treatment of malaria.
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Author Information
  • Bioresources Development Centre Katsina, National Biotechnology Development Agency, Abuja, Nigeria

  • Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria

  • Department of Biochemistry, Kaduna State University, Kaduna, Nigeria

  • Department of Microbiology, Umaru Musa Yar’adua University, Katsina, Nigeria

  • Bioresources Development Centre Katsina, National Biotechnology Development Agency, Abuja, Nigeria

  • Bioresources Development Centre Katsina, National Biotechnology Development Agency, Abuja, Nigeria

  • Bioresources Development Centre Katsina, National Biotechnology Development Agency, Abuja, Nigeria

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