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Genetic Diversity of the N’Dama Breed in Mali Using SSR Markers

Received: 22 September 2018     Accepted: 15 October 2018     Published: 13 November 2018
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Abstract

The N'Dama race, trypanotolerant and well adapted to the climatic conditions of Mali, is threatened of disappearance by the introduction of other genes by means of the artificial insemination with the exotic races or zebus. In order to adopt preservation and conservation strategies, it is important to study the genetic characteristics of the race across the country. In this study, carried out on the race in its cradle (Yanfolila district, Sikasso region), the genetic diversity of 119 N'Dama race, from the Madina-Diassa Center for Preservation, Multiplication and Dissemination of Endemic Ruminant Livestock, was evaluated with 9 microsatellite markers (SSR). A total of 60 alleles were obtained. The number of alleles varied from 2 (BM 1824) to 12 (INRA 37) with an average of 6.67 per locus. The PIC ranked from 0.39 (BM 1824) to 0.9183 (INRA 37) with an average of 0.6605. Genetic diversity ranged from 0.4293 (BM 1824) to 0.9228 (INRA) with an average of 0.6908. The 119 N'Dama races were classified into two groups I and J according to the genetic similarities revealed by the 9 SSR markers using the UPGMA method. Group J was formed with the majority (85%) of individuals and composed of four (4) clusters J1, J2, J3 and J4. The 69 Nd, 71 Nd and 72 Nd individuals showed strong dissimilar compared to other individuals in group J and formed cluster J1. Moreover, one cluster, with 15% of individuals, was belonged to Group I. The results of this study will contribute to the application of molecular tools and strengthen strategies for conservation, preservation and genetic improvement of the N’Dama race in Mali.

Published in International Journal of Animal Science and Technology (Volume 2, Issue 3)
DOI 10.11648/j.ijast.20180203.11
Page(s) 30-35
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Genetic Diversity, N'Dama, SSR Marker, Mali

References
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Cite This Article
  • APA Style

    Drissa Konaté, Diakaridia Traoré, Sognan Dao, Rokiatou Fané, Oumar Ouattara, et al. (2018). Genetic Diversity of the N’Dama Breed in Mali Using SSR Markers. International Journal of Animal Science and Technology, 2(3), 30-35. https://doi.org/10.11648/j.ijast.20180203.11

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    ACS Style

    Drissa Konaté; Diakaridia Traoré; Sognan Dao; Rokiatou Fané; Oumar Ouattara, et al. Genetic Diversity of the N’Dama Breed in Mali Using SSR Markers. Int. J. Anim. Sci. Technol. 2018, 2(3), 30-35. doi: 10.11648/j.ijast.20180203.11

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    AMA Style

    Drissa Konaté, Diakaridia Traoré, Sognan Dao, Rokiatou Fané, Oumar Ouattara, et al. Genetic Diversity of the N’Dama Breed in Mali Using SSR Markers. Int J Anim Sci Technol. 2018;2(3):30-35. doi: 10.11648/j.ijast.20180203.11

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  • @article{10.11648/j.ijast.20180203.11,
      author = {Drissa Konaté and Diakaridia Traoré and Sognan Dao and Rokiatou Fané and Oumar Ouattara and Ramata Diop and Amadou Hamadoun Babana},
      title = {Genetic Diversity of the N’Dama Breed in Mali Using SSR Markers},
      journal = {International Journal of Animal Science and Technology},
      volume = {2},
      number = {3},
      pages = {30-35},
      doi = {10.11648/j.ijast.20180203.11},
      url = {https://doi.org/10.11648/j.ijast.20180203.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijast.20180203.11},
      abstract = {The N'Dama race, trypanotolerant and well adapted to the climatic conditions of Mali, is threatened of disappearance by the introduction of other genes by means of the artificial insemination with the exotic races or zebus. In order to adopt preservation and conservation strategies, it is important to study the genetic characteristics of the race across the country. In this study, carried out on the race in its cradle (Yanfolila district, Sikasso region), the genetic diversity of 119 N'Dama race, from the Madina-Diassa Center for Preservation, Multiplication and Dissemination of Endemic Ruminant Livestock, was evaluated with 9 microsatellite markers (SSR). A total of 60 alleles were obtained. The number of alleles varied from 2 (BM 1824) to 12 (INRA 37) with an average of 6.67 per locus. The PIC ranked from 0.39 (BM 1824) to 0.9183 (INRA 37) with an average of 0.6605. Genetic diversity ranged from 0.4293 (BM 1824) to 0.9228 (INRA) with an average of 0.6908. The 119 N'Dama races were classified into two groups I and J according to the genetic similarities revealed by the 9 SSR markers using the UPGMA method. Group J was formed with the majority (85%) of individuals and composed of four (4) clusters J1, J2, J3 and J4. The 69 Nd, 71 Nd and 72 Nd individuals showed strong dissimilar compared to other individuals in group J and formed cluster J1. Moreover, one cluster, with 15% of individuals, was belonged to Group I. The results of this study will contribute to the application of molecular tools and strengthen strategies for conservation, preservation and genetic improvement of the N’Dama race in Mali.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Genetic Diversity of the N’Dama Breed in Mali Using SSR Markers
    AU  - Drissa Konaté
    AU  - Diakaridia Traoré
    AU  - Sognan Dao
    AU  - Rokiatou Fané
    AU  - Oumar Ouattara
    AU  - Ramata Diop
    AU  - Amadou Hamadoun Babana
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    PY  - 2018
    N1  - https://doi.org/10.11648/j.ijast.20180203.11
    DO  - 10.11648/j.ijast.20180203.11
    T2  - International Journal of Animal Science and Technology
    JF  - International Journal of Animal Science and Technology
    JO  - International Journal of Animal Science and Technology
    SP  - 30
    EP  - 35
    PB  - Science Publishing Group
    SN  - 2640-1312
    UR  - https://doi.org/10.11648/j.ijast.20180203.11
    AB  - The N'Dama race, trypanotolerant and well adapted to the climatic conditions of Mali, is threatened of disappearance by the introduction of other genes by means of the artificial insemination with the exotic races or zebus. In order to adopt preservation and conservation strategies, it is important to study the genetic characteristics of the race across the country. In this study, carried out on the race in its cradle (Yanfolila district, Sikasso region), the genetic diversity of 119 N'Dama race, from the Madina-Diassa Center for Preservation, Multiplication and Dissemination of Endemic Ruminant Livestock, was evaluated with 9 microsatellite markers (SSR). A total of 60 alleles were obtained. The number of alleles varied from 2 (BM 1824) to 12 (INRA 37) with an average of 6.67 per locus. The PIC ranked from 0.39 (BM 1824) to 0.9183 (INRA 37) with an average of 0.6605. Genetic diversity ranged from 0.4293 (BM 1824) to 0.9228 (INRA) with an average of 0.6908. The 119 N'Dama races were classified into two groups I and J according to the genetic similarities revealed by the 9 SSR markers using the UPGMA method. Group J was formed with the majority (85%) of individuals and composed of four (4) clusters J1, J2, J3 and J4. The 69 Nd, 71 Nd and 72 Nd individuals showed strong dissimilar compared to other individuals in group J and formed cluster J1. Moreover, one cluster, with 15% of individuals, was belonged to Group I. The results of this study will contribute to the application of molecular tools and strengthen strategies for conservation, preservation and genetic improvement of the N’Dama race in Mali.
    VL  - 2
    IS  - 3
    ER  - 

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Author Information
  • Department of Biology, Faculty of Sciences & Techniques, University of Sciences, Techniques and Technologies, Bamako, Mali

  • Department of Biology, Faculty of Sciences & Techniques, University of Sciences, Techniques and Technologies, Bamako, Mali

  • Department of Biology, Faculty of Sciences & Techniques, University of Sciences, Techniques and Technologies, Bamako, Mali

  • Department of Biology, Faculty of Sciences & Techniques, University of Sciences, Techniques and Technologies, Bamako, Mali

  • Department of Biology, Faculty of Sciences & Techniques, University of Sciences, Techniques and Technologies, Bamako, Mali

  • Department of Biology, Faculty of Sciences & Techniques, University of Sciences, Techniques and Technologies, Bamako, Mali

  • Department of Biology, Faculty of Sciences & Techniques, University of Sciences, Techniques and Technologies, Bamako, Mali

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