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Analysis of Peroxidase Activity and Total Phenol from Spathoglottis plicata Bl Plantlet Toward to Fusarium oxysporum

Received: 25 July 2016     Accepted: 14 September 2016     Published: 14 October 2016
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Abstract

Ground orchid (Spathoglottis plicata Bl) is an ornamental plant widely appreciated by the public because it has a beautiful shape, colour and flower formation. One of trigger decrease of S. plicata production caused by Fusarium wilt which is caused the Fusarium oxysporum. Using resistant S. plicata cultivars are expected to be an alternative to control the disease. A resistant S. plicatta plantlet to Fo has been initiated by in vitro selection on medium countaining fusaric acid on different concentrations. This study aims to determine the peroxidase activity and total phenol of S. plicata plantlet. Research conducted at the in vitro Laboratory, Department of Biology, Faculty of Mathematics and Natural of Sciences, University of Lampung. The study design was Completely Randomized. Data analyzed using Analysis of Variance and if significantly different followed by Least Significant Difference (LSD) test 5% significance level. The results showed that increasing the concentration of fusaric acid, it also increases the peroxidase enzyme activity and total phenol in plantlet that resistant to F. oxysporum. At a concentration of 40 ppm, peroxidase activity and the highest total phenol content are 0.536 unit/mg/second and 10.33%.

Published in International Journal of Applied Agricultural Sciences (Volume 2, Issue 6)
DOI 10.11648/j.ijaas.20160206.11
Page(s) 79-82
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Spathoglottis plicata, Induced Resistance, Fusarium oxysporum, Peroxidase Activity, Total Phenol

References
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[2] Arai M and Takeuchi M. 1993. Influence of Fusarium Wilt toxin (s) on Carnation cell. Plant Cells, Tissue and Organ Culture (34): 287–293.
[3] Bacon CW, Porter JK, Norred WP dan Leslie JF. 1996. Production of Fusaric Acid by Fusarium sp. Applied and Enviromental Microbiology. 62 (11): 4039-4043.
[4] Bouizgarne B, Bouteau HEM, Frankart C, Reboutier D, Madiona K, Pennarun AM, Monestiez M, Trouverie J, Amiar Z, Briand J, Brault M, Rona JP, Ouhdouch Y, & Hadramu EI. 2006. Early Physiological Responses of Arabidopsis thaliana Cells to Fusaric Acid: Toxic and Signallling Effects. New Phytologist 169: 209–218.
[5] Goodman RN, Zoltan K, and Milton Z, 1986. The Biochemistry and Physiology of Plant Disease. D. Van Nostrand Company, Inc. New Jersey, Toronto, London, Melbourne.
[6] Hadisutrisno, B. 1995. Taktik dan Strategi Perlindungan Tanaman Menghadapi Gangguan Penyakit Layu Fusarium. Makalah Simposium Nasional I di Purwokerto, 2-3 Maret.
[7] Khan I. A, Alam S. S, Haq A, & Jabbar A. 2005. Biochemistry of Resistance in Chicpea Against Wilt Disease Caused by Fusarium oxysporum f. sp. ciceris. Pak. J. Bot. 37: 97-104.
[8] Landa BB, Cachinero-Diaz JM, Lemanceu P, Jimenez-Diaz RM & Alabouvette C. 2002. Effect of fusaric acid and phytoanticipans on growth of rhizobacteria and Fusarium oxysporum. Canadian Journal of Microbiology 48: 971-985.
[9] Nurcahyani E, Sumardi I, Hadisutrisno B, & Suharyanto E. 2012. Supression of Development of Vanilla Foot Rot Disease (Fusarium oxysporum f. sp. vanillae) Throught In Vitro Fusaric Acid Selection. Journal of Tropical Plant Pests and Diseases. Acreditation of DIKTI No.110/DIKTI/Kep/2009. ISSN: 1411-7525. Vol. 12 /No. 1: 12-22.
[10] Palmer GD. 2011. The control of orchids. http://www.ebow.com/info_8525784_control_fusarium-wilt-orchids html. Accesed on January 20, 2016.
[11] Remotti PC, Lofler HJM & Lotten-Doting LV. 1997. Selection of cell lines and regeneration of plants resistance to fusaric acid from Gladiolus x gradiflorus c.v ‘Peteer Pear’. Euphytica 96: 237-245.
[12] Ruzin, SE. 1999. Plant Microtechnique and Microscopy. Oxford University Press, New York.
[13] Saravanan T, Bhaskaran R, and Muthusamy M. 2004. Pseudomonas fluorescens Induced Enzymological Changes in Banana Roots (cv. Rasthali) against Fusarium Wilt Disease. Plant Pathology Journal. 3: 72-80.
[14] Sticher L, Mauch-Mani B and Metraux JP. 1997. Systemic acquired resistance. Annual Review Phytopathology 35: 235-270.
[15] Toyoda H, Hasyashi H and Yamamoto K. 1984. Selection of resistant tomato calli to fusaric acid. Ann. Phytopathol. Soc. Japan 50: 538–540.
[16] Umamaheswari R and Mohanan K. V. 2009. A Study of the Asociation of Agronomic Characters in Vanilla planifolia Andrews. International Journal of Plant Breeding and Genetics 5 (1): 53-58.
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  • APA Style

    Endang Nurcahyani, Rochmah Agustrina, Erdi Suroso, Gardis Andari. (2016). Analysis of Peroxidase Activity and Total Phenol from Spathoglottis plicata Bl Plantlet Toward to Fusarium oxysporum. International Journal of Applied Agricultural Sciences, 2(6), 79-82. https://doi.org/10.11648/j.ijaas.20160206.11

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    ACS Style

    Endang Nurcahyani; Rochmah Agustrina; Erdi Suroso; Gardis Andari. Analysis of Peroxidase Activity and Total Phenol from Spathoglottis plicata Bl Plantlet Toward to Fusarium oxysporum. Int. J. Appl. Agric. Sci. 2016, 2(6), 79-82. doi: 10.11648/j.ijaas.20160206.11

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    AMA Style

    Endang Nurcahyani, Rochmah Agustrina, Erdi Suroso, Gardis Andari. Analysis of Peroxidase Activity and Total Phenol from Spathoglottis plicata Bl Plantlet Toward to Fusarium oxysporum. Int J Appl Agric Sci. 2016;2(6):79-82. doi: 10.11648/j.ijaas.20160206.11

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  • @article{10.11648/j.ijaas.20160206.11,
      author = {Endang Nurcahyani and Rochmah Agustrina and Erdi Suroso and Gardis Andari},
      title = {Analysis of Peroxidase Activity and Total Phenol from Spathoglottis plicata Bl Plantlet Toward to Fusarium oxysporum},
      journal = {International Journal of Applied Agricultural Sciences},
      volume = {2},
      number = {6},
      pages = {79-82},
      doi = {10.11648/j.ijaas.20160206.11},
      url = {https://doi.org/10.11648/j.ijaas.20160206.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijaas.20160206.11},
      abstract = {Ground orchid (Spathoglottis plicata Bl) is an ornamental plant widely appreciated by the public because it has a beautiful shape, colour and flower formation. One of trigger decrease of S. plicata production caused by Fusarium wilt which is caused the Fusarium oxysporum. Using resistant S. plicata cultivars are expected to be an alternative to control the disease. A resistant S. plicatta plantlet to Fo has been initiated by in vitro selection on medium countaining fusaric acid on different concentrations. This study aims to determine the peroxidase activity and total phenol of S. plicata plantlet. Research conducted at the in vitro Laboratory, Department of Biology, Faculty of Mathematics and Natural of Sciences, University of Lampung. The study design was Completely Randomized. Data analyzed using Analysis of Variance and if significantly different followed by Least Significant Difference (LSD) test 5% significance level. The results showed that increasing the concentration of fusaric acid, it also increases the peroxidase enzyme activity and total phenol in plantlet that resistant to F. oxysporum. At a concentration of 40 ppm, peroxidase activity and the highest total phenol content are 0.536 unit/mg/second and 10.33%.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - Analysis of Peroxidase Activity and Total Phenol from Spathoglottis plicata Bl Plantlet Toward to Fusarium oxysporum
    AU  - Endang Nurcahyani
    AU  - Rochmah Agustrina
    AU  - Erdi Suroso
    AU  - Gardis Andari
    Y1  - 2016/10/14
    PY  - 2016
    N1  - https://doi.org/10.11648/j.ijaas.20160206.11
    DO  - 10.11648/j.ijaas.20160206.11
    T2  - International Journal of Applied Agricultural Sciences
    JF  - International Journal of Applied Agricultural Sciences
    JO  - International Journal of Applied Agricultural Sciences
    SP  - 79
    EP  - 82
    PB  - Science Publishing Group
    SN  - 2469-7885
    UR  - https://doi.org/10.11648/j.ijaas.20160206.11
    AB  - Ground orchid (Spathoglottis plicata Bl) is an ornamental plant widely appreciated by the public because it has a beautiful shape, colour and flower formation. One of trigger decrease of S. plicata production caused by Fusarium wilt which is caused the Fusarium oxysporum. Using resistant S. plicata cultivars are expected to be an alternative to control the disease. A resistant S. plicatta plantlet to Fo has been initiated by in vitro selection on medium countaining fusaric acid on different concentrations. This study aims to determine the peroxidase activity and total phenol of S. plicata plantlet. Research conducted at the in vitro Laboratory, Department of Biology, Faculty of Mathematics and Natural of Sciences, University of Lampung. The study design was Completely Randomized. Data analyzed using Analysis of Variance and if significantly different followed by Least Significant Difference (LSD) test 5% significance level. The results showed that increasing the concentration of fusaric acid, it also increases the peroxidase enzyme activity and total phenol in plantlet that resistant to F. oxysporum. At a concentration of 40 ppm, peroxidase activity and the highest total phenol content are 0.536 unit/mg/second and 10.33%.
    VL  - 2
    IS  - 6
    ER  - 

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Author Information
  • Dept. of Biology, Faculty of Mathematics and Natural of Science, University of Lampung, Bandar Lampung, Indonesia

  • Dept. of Biology, Faculty of Mathematics and Natural of Science, University of Lampung, Bandar Lampung, Indonesia

  • Dept. of Agricultural Technology, Faculty of Agriculture, University of Lampung, Bandar Lampung, Indonesia

  • Dept. of Biology, Faculty of Mathematics and Natural of Science, University of Lampung, Bandar Lampung, Indonesia

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