| Peer-Reviewed

Influence of Adding Manganese to Tris Extender on some Post-Cryopreservation Semen Attributes of Holstein Bulls

Received: 14 May 2015     Accepted: 31 May 2015     Published: 1 June 2015
Views:       Downloads:
Abstract

This study was conducted to explore the adding effect of manganese (Mn+2) to Tris extender on post cooling and cryopreservation semen attributes of Holstein bulls for different periods. Seven Holstein bulls of 3.5-4.5 years old were used in this study. Pooled semen was divided into three groups. The first group was diluted with Tris only (control group, G1). While the 2nd (G2) and 3rd (G3) groups were added 0.7 and 0.9 mM Mn+2respectively. The effect of these additions on Holstein bulls semen quality was studied during different periods (cooling at 5 Celsius, 72 hrs.,1st , 2nd and 3rd post cryopreservation, PC). Addition of Mn+2 led to significant increases of total (×106) plasma membrane integrity of sperms and total function sperm fraction as compared with control groups during cooling and PC periods. It can be concluded that the addition two levels of acid Mn+2 led to enhanced PC semen quality of bulls. This will in turn improved fertility rate of cows and owner's economic income.

Published in International Journal of Applied Agricultural Sciences (Volume 1, Issue 2)
DOI 10.11648/j.ijaas.20150102.12
Page(s) 26-30
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Mn+2, Cryopreservation, Holstein Bulls Semen

References
[1] A. Kumaresan, M.R. Ansari, and A. Garg, Modulation of post-thaw sperm functions with oviductal proteins in buffaloes. Anim. Reprod. Sci. 2006,90: 73–84.
[2] S. Andrabi. Fundamental principles of cryopreservation of Bos taurus and Bos indicus bull spermatozoa. Mini review. Int. J. Agri. and Biol., 2007. 9:367-369.
[3] A. Ollero, R.D. Powers, and J. G. Alvarez, Variation of docosahexaenoic acid content in subsets of human spermatozoa at different stages of maturation: Implications for sperm lipoperoxidative damage. Mol. Reprod. Dev., 2000, 55: 326-334.
[4] K. Schuh, E.J. Cartwright, E. Jankevics, K. Bundschu, J. Liebermann, J. C. Williams, A. L. Armesilla, M. Emerson, D. Oceandy, K. P. Knobeloch and L. Neyses, Plasma membrane Ca+2 ATPase 4 is required for sperm motility and male fertility. J. Biol. Chem., 2004, 279: 28220-28226.
[5] J.F. Bilodeau, S. Chatterjee, M. A. Sired and C. Gagnon, Levels of antioxidant defenses are decreased in bovine spermatozoa after a cycle of freezing and thawing. Mol. Reprod. Dev., 2000,55:282-288.
[6] S. M. Eidan, O. H. Al-Zaidi, F.F. Ibrahim, B. A. R. Al-Timimi, W. Y. Lateef, Effect of adding catalase and glutathione reduce to Tris extender on freezing ability of Holstein bulls following different cryopreservation periods. 2015. Iraqi J. Vet. Med., 39.1n press.
[7] M. Fraczek, P. Szumala-Kakola, Jedrzejezak, M. Kamieniczna, and M. Kurpisz, Bacteria trigger oxygen radical release and sperm lipid peroxidation in in vitro model of semen inflammation. Fertil. Steril., 2007, 88: 1076-1085.
[8] J. M. Gallardo, Evaluation of antioxidant system in normal semen. Rev. Invest. Clin., 2007, 59: 42-47.
[9] Y. G. Aboua, S. S. Du Plessis, P. Reichgelt, and W. Brooks, The in vitro effects of superoxide some commercially available antioxidants and red palm oil on sperm motility. Asian J. Androl., 2009, 11: 695-702.
[10] L. A. Macmillan-Crow, and D. L. Cruthirds, Manganese superoxide dismutase in disease. Free Rad. Res., 2001,34:325-36.
[11] L. Campanella, T. Gatta and O. Ravera, Relationship between antioxidant capacity and manganese accumulation in the soft tissues of two freshwater molluscs: Unio pictorum mancus (Lamellibranchia, Unionidae) and Viviparous ater (Gastropoda, Prosobranchia). J. Limnol., 2005, 64:153-8.
[12] A. K. Bansal, Effects of antioxidants on crossbred cattle bull spermatozoa under oxidative stress. 2006, Ph. D Thesis, Punjab Agric. Univ., Ludhiana, India.
[13] S. Lapointe, I. Ahmad and M. M. Buhr, Modulation of post thaw motility, survival, calcium uptake, and fertility of bovine sperm by magnesium and manganese. J. Dairy Sci., 1996, 12: 2163–2169.
[14] A.K. Bansal, R. S. Cheema, and V. K, Gandotra. 2011. Antioxidant effect of Mn2+on capacitation and acrosome reaction of fresh and chilled cattle bull semen. Vet. Sci. Dev., 1: 69-72.
[15] S. M. Eidan, T.A. Abdulkareem, and O.A. A. Sultan, Effect of adding manganese to Tris extender on some post-cryopreservation semen characteristics of Holstein bulls. June 3rd - 6th 2015. [7th Balkan Scientific Animal Science Conference, BALNIMALCON. Sarajevo].
[16] S. Salamon, and W. M. C. Maxwell, Storage of ram semen .Anim. Reprod. Sci., 2000, 62: 77-111.
[17] R.S. Jeyendran, H. H. Van der Ven, M. Perez-Pelaez, B.G. Crab and L.J.D. Zaneveld, Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to the other semen characteristics. J. Reprod .Fertile., 1984, 70:219-225.
[18] P.M. Zavos, Hypoosmotic swelling test (HOS)/functional integrity of sperm membrane. J Ass. Reprod. Technol. Androl., 1990, 2:215-216.
[19] R. C. Johnston, G.N. Clarke, D. Yi Liu, and H. W. Gordon Baker, Assessment of the sperm quality analyzer. Fertile. Sterile., 1995, 63:1071-1076.
[20] SAS. SAS\STAT User’s Guide for Personal Computers. Release 9.1 SAS Institute Inc., Cary, N. C., USA. 2010.
[21] D. B. Duncan, Multiple range and multiple F. Tests. Biometrics., 1955, 11:1-42.
[22] R.S. Cheema, A. K. Bansal and G. S. Bilaspuri, Manganese provides antioxidant protection for sperm cryopreservation that may offer new consideration for clinical fertility. Oxidative Medicine and Cellular Longevity, 2009, 2:152-159.
[23] S. M. Eidan, and O.A. A. Sultan, Ibrahim, F. F., Hurish, K. F and Naseer, H. H. 2015. Effect of adding co-enzymes (α-lipoic acid and Q10) and manganese on post-cryopreservation semen quality characteristics of Holstein bulls. Iraqi J. Vet. Med., 39.1n press.
[24] S. M. Eidan, and O.A. A. Sultan, Effect of adding manganese on post-cryopreservation semen quality characteristics of Holstein bulls. unpublished.
[25] M. Coassin, F. Ursini, and A. Bindoli, Antioxidant effect of manganese. Arch. Biochem. Biophys., 1992, 299: 330-333.
[26] J. D. Aguirre, and V. C. Culotta, Battles with iron: Manganese in oxidative stress protection. J. Biol. Chem., 2012, 287:13541-13548.
[27] A. K. Bansal, and R. J. K. Anand, Cooperative functions of manganese and thiol redox system against oxidative stress in human spermatozoa. J. Hum. Reprod. Sci., 2009, 2:75-79.
[28] A. K. Bansal, Manganese: A potent antioxidant in semen. I. J. A. S., 2013, 3: 217-221.
[29] T. Braun, The effect of divalent cations on bovine spermatozoa Adenylate cyclase activity. J. Cycl. Nucleo. Res., 1975,1: 271-281.
[30] T. Mann, and C. L. Mann, Male Reproductive Function and Semen. Springer Verlag, New York. 1981,pp.269-336.
[31] E. M. Eddy, and D. A. O’Brien, The spermatozoon. In: The Physiology of Reproduction. E. Knobil and J. D. Neil (Eds.), Raven Press, New York. 1994, pp. 29–78.
[32] A. R. Reddi, L. T. Jensen, A. Naranuntarat, L. Rosenfeld, E. Leung, R. Shah, and V. C. Culotta, The overlapping roles of manganese and Cu\ Zn SOD in oxidative stress protection. Free Radic. Biol. Med., 2009, 46: 154-162.
[33] L. Cavallini, M. Valente, and A. Bindoli, On the mechanism of inhibition of lipid peroxidation by manganese. Inorg. Chim. Act., 1984, 91: 117-120.
[34] A. K. Bansal, and G. S. Bilaspuri, Mn2+: a potent antioxidant and stimulator of sperm capacitation and acrosome reaction in crossbred cattle bulls. Archiv fur Tierzucht, 2008, 51:149–156.
[35] G.S. Bilaspuri, and A.K. Bansal, Mn2+: A potent antioxidant and stimulator of sperm capacitation and acrosome reaction in crossbred cattle bulls. Arch. Tierz., 2008, 51: 149-158.
Cite This Article
  • APA Style

    Sajeda Mahdi Eidan, Talal Anwer Abdulkareem, Omer Amer Abd Sultan. (2015). Influence of Adding Manganese to Tris Extender on some Post-Cryopreservation Semen Attributes of Holstein Bulls. International Journal of Applied Agricultural Sciences, 1(2), 26-30. https://doi.org/10.11648/j.ijaas.20150102.12

    Copy | Download

    ACS Style

    Sajeda Mahdi Eidan; Talal Anwer Abdulkareem; Omer Amer Abd Sultan. Influence of Adding Manganese to Tris Extender on some Post-Cryopreservation Semen Attributes of Holstein Bulls. Int. J. Appl. Agric. Sci. 2015, 1(2), 26-30. doi: 10.11648/j.ijaas.20150102.12

    Copy | Download

    AMA Style

    Sajeda Mahdi Eidan, Talal Anwer Abdulkareem, Omer Amer Abd Sultan. Influence of Adding Manganese to Tris Extender on some Post-Cryopreservation Semen Attributes of Holstein Bulls. Int J Appl Agric Sci. 2015;1(2):26-30. doi: 10.11648/j.ijaas.20150102.12

    Copy | Download

  • @article{10.11648/j.ijaas.20150102.12,
      author = {Sajeda Mahdi Eidan and Talal Anwer Abdulkareem and Omer Amer Abd Sultan},
      title = {Influence of Adding Manganese to Tris Extender on some Post-Cryopreservation Semen Attributes of Holstein Bulls},
      journal = {International Journal of Applied Agricultural Sciences},
      volume = {1},
      number = {2},
      pages = {26-30},
      doi = {10.11648/j.ijaas.20150102.12},
      url = {https://doi.org/10.11648/j.ijaas.20150102.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijaas.20150102.12},
      abstract = {This study was conducted to explore the adding effect of manganese (Mn+2) to Tris extender on post cooling and cryopreservation semen attributes of Holstein bulls for different periods. Seven Holstein bulls of 3.5-4.5 years old were used in this study. Pooled semen was divided into three groups. The first group was diluted with Tris only (control group, G1). While the 2nd (G2) and 3rd (G3) groups were added 0.7 and 0.9 mM Mn+2respectively. The effect of these additions on Holstein bulls semen quality was studied during different periods (cooling at 5 Celsius, 72 hrs.,1st , 2nd and 3rd post cryopreservation, PC). Addition of Mn+2 led to significant increases of total (×106) plasma membrane integrity of sperms and total function sperm fraction as compared with control groups during cooling and PC periods. It can be concluded that the addition two levels of acid Mn+2 led to enhanced PC semen quality of bulls. This will in turn improved fertility rate of cows and owner's economic income.},
     year = {2015}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Influence of Adding Manganese to Tris Extender on some Post-Cryopreservation Semen Attributes of Holstein Bulls
    AU  - Sajeda Mahdi Eidan
    AU  - Talal Anwer Abdulkareem
    AU  - Omer Amer Abd Sultan
    Y1  - 2015/06/01
    PY  - 2015
    N1  - https://doi.org/10.11648/j.ijaas.20150102.12
    DO  - 10.11648/j.ijaas.20150102.12
    T2  - International Journal of Applied Agricultural Sciences
    JF  - International Journal of Applied Agricultural Sciences
    JO  - International Journal of Applied Agricultural Sciences
    SP  - 26
    EP  - 30
    PB  - Science Publishing Group
    SN  - 2469-7885
    UR  - https://doi.org/10.11648/j.ijaas.20150102.12
    AB  - This study was conducted to explore the adding effect of manganese (Mn+2) to Tris extender on post cooling and cryopreservation semen attributes of Holstein bulls for different periods. Seven Holstein bulls of 3.5-4.5 years old were used in this study. Pooled semen was divided into three groups. The first group was diluted with Tris only (control group, G1). While the 2nd (G2) and 3rd (G3) groups were added 0.7 and 0.9 mM Mn+2respectively. The effect of these additions on Holstein bulls semen quality was studied during different periods (cooling at 5 Celsius, 72 hrs.,1st , 2nd and 3rd post cryopreservation, PC). Addition of Mn+2 led to significant increases of total (×106) plasma membrane integrity of sperms and total function sperm fraction as compared with control groups during cooling and PC periods. It can be concluded that the addition two levels of acid Mn+2 led to enhanced PC semen quality of bulls. This will in turn improved fertility rate of cows and owner's economic income.
    VL  - 1
    IS  - 2
    ER  - 

    Copy | Download

Author Information
  • Department of Animal Resource, College of Agriculture, University of Baghdad, Baghdad, Iraq

  • Department of Animal Resource, College of Agriculture, University of Baghdad, Baghdad, Iraq

  • Department of Animal Resource, College of Agriculture, University of Baghdad, Baghdad, Iraq

  • Sections