Culture based diversity of Rhizobacteria associated with Sorghum wild relatives were identified by this study. Rhizobacteria, which include rhizosphere bacteria, rhizoplane bacteria and endophytic bacteria were isolated and identified. The isolates were also evaluated and screened for their ability to solubilize phosphates on Pikovskaya’s agar plates. One hundred twenty-six rhizobacteria were isolated on nutrient agar from the samples. Out of these Rhizobacterial isolates, 21 of them were endophyte bacteria, 40 of them were rhizosphere bacteria and 65 of them were rhizoplane bacteria. The 126 isolated rhizobacteria were clustered into 36 similar representative Morphotype groups. And, they were identified using GEN III Biolog microbial identification system. Gram negative rhizobacteria were more predominant than Gram positive rhizobacteria. Genus Pseudomonas was found as the most dominant Rhizobacterial taxa among the identified P-solubilizing Rhizobacteria and Non-P-solubilizing Rhizobacteria isolates. Forty seven percent (47.2%) of the clustered rhizobacterial isolates showed clearly visible haloes (>0.50 cm) around their colonies on Pikovskaya’s agar after seven days of incubation at 30 ± 2°C. They showed a significant difference of solubilization index (SI) [p<0.05] and ranged from 1.3 to 5.3 with a mean value of 2.4. Gram positive bacteria (Bacillus cibi) produced the largest solubilisation index when compared with the Gram-negative isolates. This study indicated that P-solubilizing rhizobacteria associated with Sorghum Wild Relative have very large phosphate solubilization index. Thus, it is recommended to include them in research programs that intend to screen P-solubilizing bacteria for microbial inoculant development.
| Published in | Frontiers in Environmental Microbiology (Volume 7, Issue 2) |
| DOI | 10.11648/j.fem.20210702.13 |
| Page(s) | 63-68 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2021. Published by Science Publishing Group |
Rhizobacterial Diversity, Sorghum Wild Relatives, P-solubilizing Rhizobacteria, Biolog
| [1] | Alawiye TT and Babalola OO. (2019). Review: Bacterial Diversity and Community Structure in Typical Plant Rhizosphere. Diversity. 11: 179. |
| [2] | Babalola O. O. (2010). Beneficial bacteria of agricultural importance. Biotechnol. Lett. 32: 1559–1570. |
| [3] | Babalola O. O., Kirby B. M., Roes-Hill L., Cook A. E., Cary S. C., Burton S. G., and Cowan D. A. (2009). Phylogenetic analysis of actinobacterial populations associated with Antarctic Dry Valley mineral soils. Environ. Microbiol. 11: 566–576. |
| [4] | Biolog’s Microbial Identification Systems User’s Guide. (2008). GEN III Microplate: Instruction for use. Biolog, Inc. Available online at www.biolog.com. |
| [5] | Costa R, Götz M, Mrotzek N, Lottmann J, Berg G, Smalla K. (2005). Effects of site and plant species on rhizosphere community structure as revealed by molecular analysis of microbial guilds. FEMS Microbiology and Ecology. 56: 236–249. |
| [6] | Edi-Premono M., Moawad A. M., Vleck P. L. G. (1996) Effect of phosphate solubilizing Pseudomonas putida on the growth of maize and its survival in the rhizosphere, Indones. J. Crop Sci. 11 (1996) 13e23. |
| [7] | Fanin N. and Bertrand I. (2016). Above ground litter quality is a better predictor than below ground microbial communities when estimating carbon mineralization along a land-use gradient. Soil Biol. Biochem. 94: 48–60. |
| [8] | Getahun A., Kiros S., Muleta D., and Assefa F. (2020). Genetic and metabolic diversities of rhizobacteria isolated from degraded soil of Ethiopia. Heliyon. 6 (2020) e05697. |
| [9] | Gupta S, Meena MK and Datta S (2014). Isolation, characterization of plant growth promoting bacteria from the plant Chlorophytum borivilianum and in-vitro screening for activity of nitrogen fixation, phospthate solubilization and IAA production. 3 (7): 1082-1090 ISSN: 2319-7706. |
| [10] | Hart MM & Trevors JT. (2005). Microbe management: Application of mycorrhyzal fungi in sustainable agriculture. Frontiers in Ecology & the Environment. 310: 533-539. |
| [11] | Hijmans R. J., Guarino L., and Mathur P. (2012). DIVA-GIS Version 7.5 Manual. LizardTech, Inc. USA. Available online at website: http://www.diva-gis.org. |
| [12] | IBM SPSS Statistics 25 Core System User’s Guide (2017). IBM Corporation 2011 Inc., Chicago, IL, USA, Available online at http://www.ibm.com/spss. |
| [13] | Jime´nez-Salgado T, Fuentes-Ramrez LE, Tapia-Herna´ ndez A, Mascarua-Esparza MA, Martnez-Romero E, Caballero-Mellado J. (1997). Coffea arabica L., a new host plant for Acetobacter diazotrophicus and isolation of nitrogen-fixing acetobacteria. Applied Environmental Microbiology. 63 (9): 3676–3683. |
| [14] | Lucy, M., Reed, E. & Glick, B. R. (2004). Applications of free living plant growth-promoting rhizobacteria. Antonie van Leeuwenhoek. 86: 1-25. |
| [15] | Midekssa M. J., Löscher C. R., Schmitz R. A. and Assefa F. (2016) Phosphate solubilization and multiple plant growth promoting properties of rhizobacteria isolated from chickpea (Cicer aeritinum L.) producing areas of Ethiopia. Vol. 15 (35): 1899-1912, 31 DOI: 10.5897/AJB2015.15172. |
| [16] | Monk J, Gerard E, Young S, Widdup K and O’Callaghan M (2009). Isolation and identification of plant growth-promoting bacteria. Proceedings of the New Zealand Grassland Association. 7: 211-216. |
| [17] | Muleta D., Fassil A., Börjesson E., and Granhall U. (January 2013). Phosphate-solubilising rhizobacteria associated with Coffea arabica L. in natural coffee forests of southwestern Ethiopia Journal of the Saudi Society of Agricultural Sciences. 12 (1): 73-84. |
| [18] | Muleta D., Assefa F., Hjort K., Roos S. and Granhall U. (April 2009). Characterization of Rhizobacteria Isolated from Wild Coffea arabica L. Available online at https://doi.org/10.1002/elsc.200700031 |
| [19] | Philippot L., Raaijmakers J., Lemanceau P., and Van Der Putten W. (2013). Going back to the roots: The microbial ecology of the rhizosphere. Nat. Rev. Microbiol. 11: 789. |
| [20] | Pikovskaya, R. I. (1948). Mobilization of phosphorus in soil connection with the vital activity of some microbial species. Microbiologiya. 17: 362–370. |
| [21] | Preece C. and Penuelas J. (2016). Rhizo-deposition under drought and consequences for soil communities and ecosystem resilience. Plant Soil. 409: 1–17. |
| [22] | Raj DP and Cherian N (2013) Isolation and characterization of rhizobacteria from forest soils for in vitro study on crop growth enhancement. INT J CURR SCI E. 9: 19-23. |
| [23] | Rodríguez H, Fraga R, Gonzalez T & Bashan Y. (2006). Genetics of phosphate solubilization and its potential applications for improving plant growth-promoting bacteria. Plant & Soil. 287: 15-21. |
| [24] | Shukla A. K. (2019). Ecology and diversity of plant growth promoting rhizobacteria in agricultural landscape: Chapter 1 PGPR Amelioration Sustainable Agriculture ISNB 978- 0-12-815879-1. https://doi.org/10.1016/B978-0-12-815879-1.00001-X. |
| [25] | Teshome B., Abatineh E and Ayinalem E. (2019). In vitro Screening and Identification of P-Solubilizing Rhizobacteria Associated with Sorghum bicolor L. Agri Res & Tech: Open Access J. 20 (2): 556122. |
| [26] | Thanh DTN and Diep CN. (2014). Isolation and identification of rhizospheric bacteria in Acrisols of maize (Zea mays L.) in the eastern of South Vietnam. American Journal of Life Sciences. 2 (2): 82-89 |
| [27] | Vessey JK. (2003). Plant growth promoting rhizobacteria as biofertilizers. Plant & Soil. 255: 571-586. |
| [28] | Yamaoka-Yano DM, Amorim Epr, Valarini PJ, Melo IS, Kosako Y. (1988). Characterization of rhizobacteria from Citrus rhizosphere to control Phytophthora nicotinae var. parasitica and P. citrophthora. Nature Biotechnology. 6 (3): 282-286. |
| [29] | Zhang X., Zhang R., Gao J., Wang X., Fan F., Ma X., Yin H., Zhang C., Feng K., and Deng Y. (2017). Thirty-one years of rice-rice-green manure rotations shape the rhizosphere microbial community and enrich beneficial bacteria. Soil Biol. Biochem. 104: 208–217. |
APA Style
Eleni Belay, Befekadu Teshome. (2021). Diversity of Rhizobacteria Associated with Sorghum Wild Relatives in Assosa Zone of Ethiopia and Their Ability to Solubilize Phosphate. Frontiers in Environmental Microbiology, 7(2), 63-68. https://doi.org/10.11648/j.fem.20210702.13
ACS Style
Eleni Belay; Befekadu Teshome. Diversity of Rhizobacteria Associated with Sorghum Wild Relatives in Assosa Zone of Ethiopia and Their Ability to Solubilize Phosphate. Front. Environ. Microbiol. 2021, 7(2), 63-68. doi: 10.11648/j.fem.20210702.13
AMA Style
Eleni Belay, Befekadu Teshome. Diversity of Rhizobacteria Associated with Sorghum Wild Relatives in Assosa Zone of Ethiopia and Their Ability to Solubilize Phosphate. Front Environ Microbiol. 2021;7(2):63-68. doi: 10.11648/j.fem.20210702.13
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.fem.20210702.13,
author = {Eleni Belay and Befekadu Teshome},
title = {Diversity of Rhizobacteria Associated with Sorghum Wild Relatives in Assosa Zone of Ethiopia and Their Ability to Solubilize Phosphate},
journal = {Frontiers in Environmental Microbiology},
volume = {7},
number = {2},
pages = {63-68},
doi = {10.11648/j.fem.20210702.13},
url = {https://doi.org/10.11648/j.fem.20210702.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20210702.13},
abstract = {Culture based diversity of Rhizobacteria associated with Sorghum wild relatives were identified by this study. Rhizobacteria, which include rhizosphere bacteria, rhizoplane bacteria and endophytic bacteria were isolated and identified. The isolates were also evaluated and screened for their ability to solubilize phosphates on Pikovskaya’s agar plates. One hundred twenty-six rhizobacteria were isolated on nutrient agar from the samples. Out of these Rhizobacterial isolates, 21 of them were endophyte bacteria, 40 of them were rhizosphere bacteria and 65 of them were rhizoplane bacteria. The 126 isolated rhizobacteria were clustered into 36 similar representative Morphotype groups. And, they were identified using GEN III Biolog microbial identification system. Gram negative rhizobacteria were more predominant than Gram positive rhizobacteria. Genus Pseudomonas was found as the most dominant Rhizobacterial taxa among the identified P-solubilizing Rhizobacteria and Non-P-solubilizing Rhizobacteria isolates. Forty seven percent (47.2%) of the clustered rhizobacterial isolates showed clearly visible haloes (>0.50 cm) around their colonies on Pikovskaya’s agar after seven days of incubation at 30 ± 2°C. They showed a significant difference of solubilization index (SI) [p<0.05] and ranged from 1.3 to 5.3 with a mean value of 2.4. Gram positive bacteria (Bacillus cibi) produced the largest solubilisation index when compared with the Gram-negative isolates. This study indicated that P-solubilizing rhizobacteria associated with Sorghum Wild Relative have very large phosphate solubilization index. Thus, it is recommended to include them in research programs that intend to screen P-solubilizing bacteria for microbial inoculant development.},
year = {2021}
}
TY - JOUR T1 - Diversity of Rhizobacteria Associated with Sorghum Wild Relatives in Assosa Zone of Ethiopia and Their Ability to Solubilize Phosphate AU - Eleni Belay AU - Befekadu Teshome Y1 - 2021/05/21 PY - 2021 N1 - https://doi.org/10.11648/j.fem.20210702.13 DO - 10.11648/j.fem.20210702.13 T2 - Frontiers in Environmental Microbiology JF - Frontiers in Environmental Microbiology JO - Frontiers in Environmental Microbiology SP - 63 EP - 68 PB - Science Publishing Group SN - 2469-8067 UR - https://doi.org/10.11648/j.fem.20210702.13 AB - Culture based diversity of Rhizobacteria associated with Sorghum wild relatives were identified by this study. Rhizobacteria, which include rhizosphere bacteria, rhizoplane bacteria and endophytic bacteria were isolated and identified. The isolates were also evaluated and screened for their ability to solubilize phosphates on Pikovskaya’s agar plates. One hundred twenty-six rhizobacteria were isolated on nutrient agar from the samples. Out of these Rhizobacterial isolates, 21 of them were endophyte bacteria, 40 of them were rhizosphere bacteria and 65 of them were rhizoplane bacteria. The 126 isolated rhizobacteria were clustered into 36 similar representative Morphotype groups. And, they were identified using GEN III Biolog microbial identification system. Gram negative rhizobacteria were more predominant than Gram positive rhizobacteria. Genus Pseudomonas was found as the most dominant Rhizobacterial taxa among the identified P-solubilizing Rhizobacteria and Non-P-solubilizing Rhizobacteria isolates. Forty seven percent (47.2%) of the clustered rhizobacterial isolates showed clearly visible haloes (>0.50 cm) around their colonies on Pikovskaya’s agar after seven days of incubation at 30 ± 2°C. They showed a significant difference of solubilization index (SI) [p<0.05] and ranged from 1.3 to 5.3 with a mean value of 2.4. Gram positive bacteria (Bacillus cibi) produced the largest solubilisation index when compared with the Gram-negative isolates. This study indicated that P-solubilizing rhizobacteria associated with Sorghum Wild Relative have very large phosphate solubilization index. Thus, it is recommended to include them in research programs that intend to screen P-solubilizing bacteria for microbial inoculant development. VL - 7 IS - 2 ER -
Information
Email: