Plant-microbe interactions in petroleum product-contaminated soils were examined under field conditions, to analyze the effects of environmental factors on rhizoremediation. Different plant types (elephant grass, cassava, carpet grass, Indian bamboo, fern, palm and a mixture of these plants) were considered. The abundance of effective indigenous microorganisms (EIM) and remediation time were also considered. Eight soil samples were collected from crude oil-contaminated sites of Ogoni, Rivers State, Nigeria. Total viable counts were taken using spread plate methods. Hydrocarbon utilizing microorganisms using mineral salt agar with crude oil vapor phase gave counts in the range of 102 – 108. Gas Chromatographic – FID methods gave results of 9 field samples for TPH to range from 0.96 mg/kg for control to 4,650 mg/kg for oil well 3 without vegetation. Polyaromatic hydrocarbon (PAH) of 9 field samples ranged from 0.26 mg/kg for control to 30.066 mg/kg depending on the extent of pollution of the site. Mixed plant population showed abundance of EIM (108) and greater TPH and PAH removal. The effect of time on the concentration of TPH and PAH in the different samples show that significant difference exist, P – value = 0.420. Microbial counts are significantly different over time, also microbial counts are significantly different in the different samples (F-value = 3.15, 2.76, 2.53, 2.1, 1.92 and 1.7) for rows, columns and layers. Local Nigerian plants could promote rhizoremediation of crude oil polluted soils.
| Published in | Frontiers in Environmental Microbiology (Volume 5, Issue 2) |
| DOI | 10.11648/j.fem.20190502.11 |
| Page(s) | 36-47 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2019. Published by Science Publishing Group |
Rhizoremediation, Microorganisms, TPH, PAH, Microbial Counts, Gas Chromatographic, Remediation
| [1] | Ayotamuno, M. J., Kogbara, R. B. and Ogaji, S. O. T. (2008). Bioremediation of crude oil polluted agricultural soil at Port Harcourt, Nigeria. Applied Energy 8 (11): 1249-1257. |
| [2] | Aprill, W. and Sims, R. C. (1990). Evaluation of the use of prairie grasses for Stimulating Polycyclic aromatic hydrocarbon treatment in soil. Chemosphere 20 (1-2): 253-265. |
| [3] | APHA (1998). Standard Methods for Examination of Water and Waste Water. 20th ed. APHA-AWWA/WPCP. Washington D C. |
| [4] | Bogan, B. W. Lahner, L. M., Sullivan, W. R. and Patere, K. J. R (2003). Degradation of straight chain aliphatic and high molecular weight polycyclic aromatic hydrocarbons by a strain of Mycobacterium austroafricarium. Journal of Applied Microbiology 94: 230-239. |
| [5] | Brand, J., Lugtenberg, B. J. J., Glandorf, D. C. M., Bakker, P. A. H. M., Schippers, B., and de Weger, L. A. (1990). Isolation and characterization of a superior potato root-colonizing Pseudomonas strain. In: Plant Growth Promoting Rhizobacteria-Progress and Prospects. C. Keel, B. Koller, and G. De’fago, eds. IOBC/WPRS Bull. XIV-8. Interlaken, Switzerland. Pp 350-354. |
| [6] | Cheesbrough, M (2000). Biochemical testing of Microorganisms In: Medical Laboratory Manual for Tropical Countries. Vol 11: Microbiology. Pp 58 – 69. |
| [7] | Chiapusio, G., Pujol, S., Toussaint, M. L., Badot, P. M., and Binet, P. (2007). Phenanthrene toxicity and dissipation in rhizosphere of grassland plants (Loliumperenne L. and Trifoliumpratense L.) in three spiked soils. Plant Soil 294:103-112. |
| [8] | Chikere, C. B., Okpokwasili, G. C. and Chikere, B. O. (2009). Bacterial diversity in a tropical crude oil polluted soil undergoing bioremediation. African Journal of Biotechnology. 8 (11): 2535-2540. |
| [9] | Daane, L. L., Harjono. I., Zylstra, G. J. and Häggblom, M. M. (2001). Isolation and characterization of polycyclic aromatic hydrocarbon-degrading bacteria associated with the rhizosphere of salt marsh plants. Applied Environmetal Microbioliology 67: 2683-2691. |
| [10] | Hamamura, N., Oslon, S. H., Ward, D. M. and Inskeep W. P (2006) Microbial population dynamics- associated with crude oil biodegadation in diverse soils. Applied Enviromental Microbiology. 72: 6316- 6324. |
| [11] | Jobson, A., McLauglin, M., Cook, F. D. and Westlake, D. W. S. (1974). Effects of amendments on the microbial utilization of oil appliced to soil. Applied Microbiology, 27: 166-171. |
| [12] | Kirk, J. L., Beaudette, L. A., Hart, M., Moutoglis, P., Klironomos, J. N., Lee, H. and Trevors, J. T. (2004). Methods of studying soil microbial diversity. Journal of Microbiological Methods 58: 169-188. |
| [13] | Kirk, J. L., Klironomos, J. N., Lee, H. and Tervors, J. T. (2005). The effects of perennial ryegrass and alfalfa on microbial abundance and diversity in petroleum contaminated soil. Environmental Pollution 133: 455-465. |
| [14] | Kiyohara, H., and Nagao, K. (1977). Enzymatic conversion of 1-hydroxyl -z-napthoate in phenanthere-grown Aeromonas sp. 545PI. Agricultural. Biolology and Chemistry 41:705. |
| [15] | Kuiper, I., Kravchenko, L. V., Bloemberg, G. V., and Lugtenberg, B. J. J. (2003). Pseudomonas putida Strain PCL 1444, selected for efficient root colonizatioand naphthalene degradation, effectively utilizes root exudate components. Molecular Plant Microbe, 15:734-741. |
| [16] | Liste, H. H. and Prutz, I. (2006). Plant performance, dioxygenase-expressing rhizosphere bacteria, and biodegradation of weathered hydrocarbons in contaiminated soil. Chemosphere 62: 1411 – 1420. |
| [17] | Macnaughton, S. J., Stephen, J. R., venos a A. O., Davis, G. A., Chang, Y. J. and White, D. C (1999). Microbial population changes during biovemediation of an experimentally oil spill. Applied Environmental Microbiology. 65: 3566-3574. |
| [18] | Macnaughton, S. J., Stephen, J. R., venos a A. O., Davis, G. A., Chang, Y. J. and White, D. C (1999). Microbial population changes during biovemediation of an experimentally oil spill. Applied Environmental Microbiology. 65: 3566-3574. |
| [19] | Margesim, R and Schinner, F. (2001). Bioremediation (natural attenuation) and Biostimulation) of diesel oil-contaminated soil in an alpine glacier sking area. Applied Environmental Microbiology. 67(7): 3127. |
| [20] | Margesim, R and Schinner, F. (2001). Bioremediation (natural attenuation) and Biostimulation) of diesel oil-contaminated soil in an alpine glacier sking area. Applied Environmental Microbiology. 67(7): 3127. |
| [21] | Mills, A. L., Breuil, C., and R. R. Colwell (1978). Enumeration of petroleum degrading marine and estuarine microorganisms by the most probable number method. Canadian Journal of Microbiology. 24: 552 – 557. |
| [22] | Okpokwasili, G. C. and Odokuma, L. O. (1994). Tolerance of Nitrobacter to toxicity of some Nigerian crude oils. Bulletin of Environmental Contamination and Toxicology, 52: 388-395. |
| [23] | Okpokwasili, G. S. C. (2006). Microbes and the environmental challenge. University of Port Harcourt Press, Port Harcourt. Inaugural lecture series No. 53. |
| [24] | Okpokwasili, G. S. C. and Okorie, B. B. (1988). Biodegradation potentials of microorganisms isolated from car engine lubricating oil – Trib International. 21: 215 – 220. |
| [25] | Philip, L. A. (2008). The relationship between plants and their root-associated microbial communities in hydrocarbon phytoremediation systems. University of Saskatchewan, Saskatcheian, S7N 5A8. Ph.D thesis. |
| [26] | Quatrini, P., Scaglione, G., De Pasquale, C., Rella, S. and Puglia, A. M. (2008). Isolation of Gram-positive n- alkane degrade from hydrocarbon contaminated mediterevenean shore line. Journal of Applied Microbiology. 104: 251- 259. |
| [27] | Reddy, B. R. and Sethumathan, N. (1994). Mineralization of p-nitrophenol in the rhizosphere of rice. Agricultural Ecosystem Environvironment 47: 313-317. |
| [28] | Rehman, K., Noll, H. T., Steinberg, C. E. and Kettrup, A. A. (2003). Pyrene Degradation by Mycobacterium sp. Strain KR2. Chemosphere 36: 2977. |
| [29] | Reilley, K. A., Banks, M. K. and Schwab, A. P. (1996). Organic chemicals in the environment: dissipation of polycyclic aromatic hydrocarbons in the rhizosphere. Journal of Environmental Quality. 25: 212-219. |
| [30] | Siciliano, S. D., Germida, J. J. and Greer, C. W. (2003). Changes in microbial community composition and function during polyaromatic hydrocarbon phytoremediation field trial. Applied and Environmental Microbiology, 69:483-489. |
| [31] | Sugiura, K., Ishihara, M., Shimauchi, T. and Harayama, S. (1997) Physiochemical properties and biodegradability of crude oil. Environmental Science and Technolnology 31: 45-51. |
| [32] | Talaro, K. P. and Talaro, A. (1998). Foundations in Microbiology. ISBN – 10:0697354520, ISBN – 13-9780697354525McGrawhillCompannies. |
| [33] | U.S. Environmental Protection Agency (2007). New test methods online. Extraction of Solid and Aqueus Samples for Chemical Agents. July, 2007. www.usepa.govhttp//www.epa.gov/osw/hazard/testmethods/sw846/new_meth.htm#3572 |
| [34] | Umeaku, C. N. (2008). Biochemical Testing of Microorganisms In: Laboratory Practice for Tertiary Institutions. Odus Press, Enugu. ISBN 978-38379-7-4. |
| [35] | Van Aken, B., Peres, C., Doty, S., Yoon, L. and Schnoor, J. (2004). Methylobacteriumpopuli specie, a novel aerobic, pink-pigmented, facultative methylotrophic, methane-utilising bacterium isolated from poplar trees (Populus deltoids x nigra DN 34). Evolution Microbiology 54: 1191-1196. |
| [36] | White Jr, P. M., Wolf, D. C., Thoma, G. J. and Reynolds, C. M. (2002). Phytoremediation of alkylated polycyclic aromatic hydrocarbons in a crude oil-contaminated soil. Water, Air, Soil Pollution 169: 207-220. |
| [37] | Yateem, A., M. T. Balba, A. S. El-Nawawy and N. Al-Awadhi, (2000). Plants-associated Microflora and the remediation of oil contaminated soil. International Journal of Phytoremediation 2: 183-191. |
APA Style
Umeaku Chinyelu Nkiru, Okpokwasili Gideon Chijioke, Iwuala Moses, Nwigwe Harriet Chinyelu, Chris-Umeaku Chiamaka Ijeoma. (2019). Role of Plant-Microbe Interactions in Rhizoremediation of Petroleum Product-Polluted Nigerian Soils. Frontiers in Environmental Microbiology, 5(2), 36-47. https://doi.org/10.11648/j.fem.20190502.11
ACS Style
Umeaku Chinyelu Nkiru; Okpokwasili Gideon Chijioke; Iwuala Moses; Nwigwe Harriet Chinyelu; Chris-Umeaku Chiamaka Ijeoma. Role of Plant-Microbe Interactions in Rhizoremediation of Petroleum Product-Polluted Nigerian Soils. Front. Environ. Microbiol. 2019, 5(2), 36-47. doi: 10.11648/j.fem.20190502.11
AMA Style
Umeaku Chinyelu Nkiru, Okpokwasili Gideon Chijioke, Iwuala Moses, Nwigwe Harriet Chinyelu, Chris-Umeaku Chiamaka Ijeoma. Role of Plant-Microbe Interactions in Rhizoremediation of Petroleum Product-Polluted Nigerian Soils. Front Environ Microbiol. 2019;5(2):36-47. doi: 10.11648/j.fem.20190502.11
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.fem.20190502.11,
author = {Umeaku Chinyelu Nkiru and Okpokwasili Gideon Chijioke and Iwuala Moses and Nwigwe Harriet Chinyelu and Chris-Umeaku Chiamaka Ijeoma},
title = {Role of Plant-Microbe Interactions in Rhizoremediation of Petroleum Product-Polluted Nigerian Soils},
journal = {Frontiers in Environmental Microbiology},
volume = {5},
number = {2},
pages = {36-47},
doi = {10.11648/j.fem.20190502.11},
url = {https://doi.org/10.11648/j.fem.20190502.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20190502.11},
abstract = {Plant-microbe interactions in petroleum product-contaminated soils were examined under field conditions, to analyze the effects of environmental factors on rhizoremediation. Different plant types (elephant grass, cassava, carpet grass, Indian bamboo, fern, palm and a mixture of these plants) were considered. The abundance of effective indigenous microorganisms (EIM) and remediation time were also considered. Eight soil samples were collected from crude oil-contaminated sites of Ogoni, Rivers State, Nigeria. Total viable counts were taken using spread plate methods. Hydrocarbon utilizing microorganisms using mineral salt agar with crude oil vapor phase gave counts in the range of 102 – 108. Gas Chromatographic – FID methods gave results of 9 field samples for TPH to range from 0.96 mg/kg for control to 4,650 mg/kg for oil well 3 without vegetation. Polyaromatic hydrocarbon (PAH) of 9 field samples ranged from 0.26 mg/kg for control to 30.066 mg/kg depending on the extent of pollution of the site. Mixed plant population showed abundance of EIM (108) and greater TPH and PAH removal. The effect of time on the concentration of TPH and PAH in the different samples show that significant difference exist, P – value = 0.420. Microbial counts are significantly different over time, also microbial counts are significantly different in the different samples (F-value = 3.15, 2.76, 2.53, 2.1, 1.92 and 1.7) for rows, columns and layers. Local Nigerian plants could promote rhizoremediation of crude oil polluted soils.},
year = {2019}
}
TY - JOUR T1 - Role of Plant-Microbe Interactions in Rhizoremediation of Petroleum Product-Polluted Nigerian Soils AU - Umeaku Chinyelu Nkiru AU - Okpokwasili Gideon Chijioke AU - Iwuala Moses AU - Nwigwe Harriet Chinyelu AU - Chris-Umeaku Chiamaka Ijeoma Y1 - 2019/04/12 PY - 2019 N1 - https://doi.org/10.11648/j.fem.20190502.11 DO - 10.11648/j.fem.20190502.11 T2 - Frontiers in Environmental Microbiology JF - Frontiers in Environmental Microbiology JO - Frontiers in Environmental Microbiology SP - 36 EP - 47 PB - Science Publishing Group SN - 2469-8067 UR - https://doi.org/10.11648/j.fem.20190502.11 AB - Plant-microbe interactions in petroleum product-contaminated soils were examined under field conditions, to analyze the effects of environmental factors on rhizoremediation. Different plant types (elephant grass, cassava, carpet grass, Indian bamboo, fern, palm and a mixture of these plants) were considered. The abundance of effective indigenous microorganisms (EIM) and remediation time were also considered. Eight soil samples were collected from crude oil-contaminated sites of Ogoni, Rivers State, Nigeria. Total viable counts were taken using spread plate methods. Hydrocarbon utilizing microorganisms using mineral salt agar with crude oil vapor phase gave counts in the range of 102 – 108. Gas Chromatographic – FID methods gave results of 9 field samples for TPH to range from 0.96 mg/kg for control to 4,650 mg/kg for oil well 3 without vegetation. Polyaromatic hydrocarbon (PAH) of 9 field samples ranged from 0.26 mg/kg for control to 30.066 mg/kg depending on the extent of pollution of the site. Mixed plant population showed abundance of EIM (108) and greater TPH and PAH removal. The effect of time on the concentration of TPH and PAH in the different samples show that significant difference exist, P – value = 0.420. Microbial counts are significantly different over time, also microbial counts are significantly different in the different samples (F-value = 3.15, 2.76, 2.53, 2.1, 1.92 and 1.7) for rows, columns and layers. Local Nigerian plants could promote rhizoremediation of crude oil polluted soils. VL - 5 IS - 2 ER -
Information
Email: