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Identification of Antibiotic Resistant Gram-Negative Bacteria in a Popular Street-Food Item (Chatpati) in Dhaka University Campus, Bangladesh

Received: 7 March 2018     Accepted: 29 March 2018     Published: 7 May 2018
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Abstract

Presence of antibiotic resistant bacteria in street-foods has become a global health concern. In this study, we have thoroughly investigated the bacteriological quality of Chatpati (CHT) samples collected from 7 different locations of Dhaka University campus. Various selective and differential media, Gram staining and biochemical tests were carried out for isolation, enumeration and characterization of the isolates. The total bacterial count varied in samples from 7 locations ranging from 1.1x103 to 4.1x106 CFU/g. Klebsiella spp., Escherichia coli (E. coli), Enterobacter spp., Vibrio spp., Proteus spp., Pseudomonas aeruginosa and Aeromonas spp. were identified in the collected CHT samples. Isolates were then subjected to antibiotic susceptibility test. For antibiogram profiling, Ampicillin, Colistin, Chloramphenicol, Ceftriaxone, Ciprofloxacin and Nitrofurantoin were employed in this study. Multiple antibiotic resistance index (MRI%) of the isolates were found to be ranged from 16.67 to 66.67%. Majority of the isolates showed resistance against Ampicillin and Nitrofurantoin. Therefore, it may be concluded that the consumption of CHT from this area may be severely detrimental to health due to the presence of antibiotic resistant bacteria.

Published in Frontiers in Environmental Microbiology (Volume 4, Issue 2)
DOI 10.11648/j.fem.20180402.15
Page(s) 75-80
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Antibiotic Resistant, Street-Food, Chatpati, Bacterial Count, Antibiogram

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Cite This Article
  • APA Style

    Moumita Dey, Mansura Mokbul, Ila Ismail, Sharmin Rumi Alim. (2018). Identification of Antibiotic Resistant Gram-Negative Bacteria in a Popular Street-Food Item (Chatpati) in Dhaka University Campus, Bangladesh. Frontiers in Environmental Microbiology, 4(2), 75-80. https://doi.org/10.11648/j.fem.20180402.15

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    ACS Style

    Moumita Dey; Mansura Mokbul; Ila Ismail; Sharmin Rumi Alim. Identification of Antibiotic Resistant Gram-Negative Bacteria in a Popular Street-Food Item (Chatpati) in Dhaka University Campus, Bangladesh. Front. Environ. Microbiol. 2018, 4(2), 75-80. doi: 10.11648/j.fem.20180402.15

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    AMA Style

    Moumita Dey, Mansura Mokbul, Ila Ismail, Sharmin Rumi Alim. Identification of Antibiotic Resistant Gram-Negative Bacteria in a Popular Street-Food Item (Chatpati) in Dhaka University Campus, Bangladesh. Front Environ Microbiol. 2018;4(2):75-80. doi: 10.11648/j.fem.20180402.15

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  • @article{10.11648/j.fem.20180402.15,
      author = {Moumita Dey and Mansura Mokbul and Ila Ismail and Sharmin Rumi Alim},
      title = {Identification of Antibiotic Resistant Gram-Negative Bacteria in a Popular Street-Food Item (Chatpati) in Dhaka University Campus, Bangladesh},
      journal = {Frontiers in Environmental Microbiology},
      volume = {4},
      number = {2},
      pages = {75-80},
      doi = {10.11648/j.fem.20180402.15},
      url = {https://doi.org/10.11648/j.fem.20180402.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20180402.15},
      abstract = {Presence of antibiotic resistant bacteria in street-foods has become a global health concern. In this study, we have thoroughly investigated the bacteriological quality of Chatpati (CHT) samples collected from 7 different locations of Dhaka University campus. Various selective and differential media, Gram staining and biochemical tests were carried out for isolation, enumeration and characterization of the isolates. The total bacterial count varied in samples from 7 locations ranging from 1.1x103 to 4.1x106 CFU/g. Klebsiella spp., Escherichia coli (E. coli), Enterobacter spp., Vibrio spp., Proteus spp., Pseudomonas aeruginosa and Aeromonas spp. were identified in the collected CHT samples. Isolates were then subjected to antibiotic susceptibility test. For antibiogram profiling, Ampicillin, Colistin, Chloramphenicol, Ceftriaxone, Ciprofloxacin and Nitrofurantoin were employed in this study. Multiple antibiotic resistance index (MRI%) of the isolates were found to be ranged from 16.67 to 66.67%. Majority of the isolates showed resistance against Ampicillin and Nitrofurantoin. Therefore, it may be concluded that the consumption of CHT from this area may be severely detrimental to health due to the presence of antibiotic resistant bacteria.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Identification of Antibiotic Resistant Gram-Negative Bacteria in a Popular Street-Food Item (Chatpati) in Dhaka University Campus, Bangladesh
    AU  - Moumita Dey
    AU  - Mansura Mokbul
    AU  - Ila Ismail
    AU  - Sharmin Rumi Alim
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    DO  - 10.11648/j.fem.20180402.15
    T2  - Frontiers in Environmental Microbiology
    JF  - Frontiers in Environmental Microbiology
    JO  - Frontiers in Environmental Microbiology
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    PB  - Science Publishing Group
    SN  - 2469-8067
    UR  - https://doi.org/10.11648/j.fem.20180402.15
    AB  - Presence of antibiotic resistant bacteria in street-foods has become a global health concern. In this study, we have thoroughly investigated the bacteriological quality of Chatpati (CHT) samples collected from 7 different locations of Dhaka University campus. Various selective and differential media, Gram staining and biochemical tests were carried out for isolation, enumeration and characterization of the isolates. The total bacterial count varied in samples from 7 locations ranging from 1.1x103 to 4.1x106 CFU/g. Klebsiella spp., Escherichia coli (E. coli), Enterobacter spp., Vibrio spp., Proteus spp., Pseudomonas aeruginosa and Aeromonas spp. were identified in the collected CHT samples. Isolates were then subjected to antibiotic susceptibility test. For antibiogram profiling, Ampicillin, Colistin, Chloramphenicol, Ceftriaxone, Ciprofloxacin and Nitrofurantoin were employed in this study. Multiple antibiotic resistance index (MRI%) of the isolates were found to be ranged from 16.67 to 66.67%. Majority of the isolates showed resistance against Ampicillin and Nitrofurantoin. Therefore, it may be concluded that the consumption of CHT from this area may be severely detrimental to health due to the presence of antibiotic resistant bacteria.
    VL  - 4
    IS  - 2
    ER  - 

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Author Information
  • Department of Food Technology and Nutrition Science, Noakhali Science and Technology University, Noakhali, Bangladesh

  • Department of Food Technology and Nutrition Science, Noakhali Science and Technology University, Noakhali, Bangladesh

  • Institute of Nutrition and Food Science, University of Dhaka, Dhaka, Bangladesh

  • Institute of Nutrition and Food Science, University of Dhaka, Dhaka, Bangladesh

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