The research was about characterization of extraceluller chitinase produced by Bacillus licheniformis JP2 from Penen Hot Springs, North Sumatera. Precipitation of crude chitinase was performed with different levels of ammonium sulphate. Chitinase activity was determined colorimetrically by detecting the amount of N-acetylglucosamine (GlcNAc) released from colloidal chitin substrate with N-acetylglucosamine. Characterization of chitinase activity was measured at different pH, different temperature, Km and Vmax values. The optimum activity of chitinase was produced 4 days after incubation with chitinase activity of 0.0063 U/ml. Optimum chitinase activity was higher after 50% ammonium sulphate precipitation with activity of 0.0087 U/ml followed by decreasing in activity supernatant with activity of 0.0012 U/ml. Chitinase activity increased at pH 6 and at 60°C by 0.05066 U/ml with specific activity 3.8113 U/mg after dialysis. Km and Vmax values were 0.321 mg/ml and 71.429 µg, respectively.
| Published in | Frontiers in Environmental Microbiology (Volume 2, Issue 5) |
| DOI | 10.11648/j.fem.20160205.11 |
| Page(s) | 24-27 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2016. Published by Science Publishing Group |
Bacillus licheniformis, Chitinase, Thermophilic
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APA Style
Jane Melita Keliat, Dwi Suryanto, Erman Munir. (2016). Characterization of Extraceluller Chitinase Produced by Bacillus licheniformis JP2 from Penen Hot Springs, North Sumatera. Frontiers in Environmental Microbiology, 2(5), 24-27. https://doi.org/10.11648/j.fem.20160205.11
ACS Style
Jane Melita Keliat; Dwi Suryanto; Erman Munir. Characterization of Extraceluller Chitinase Produced by Bacillus licheniformis JP2 from Penen Hot Springs, North Sumatera. Front. Environ. Microbiol. 2016, 2(5), 24-27. doi: 10.11648/j.fem.20160205.11
AMA Style
Jane Melita Keliat, Dwi Suryanto, Erman Munir. Characterization of Extraceluller Chitinase Produced by Bacillus licheniformis JP2 from Penen Hot Springs, North Sumatera. Front Environ Microbiol. 2016;2(5):24-27. doi: 10.11648/j.fem.20160205.11
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Download@article{10.11648/j.fem.20160205.11,
author = {Jane Melita Keliat and Dwi Suryanto and Erman Munir},
title = {Characterization of Extraceluller Chitinase Produced by Bacillus licheniformis JP2 from Penen Hot Springs, North Sumatera},
journal = {Frontiers in Environmental Microbiology},
volume = {2},
number = {5},
pages = {24-27},
doi = {10.11648/j.fem.20160205.11},
url = {https://doi.org/10.11648/j.fem.20160205.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20160205.11},
abstract = {The research was about characterization of extraceluller chitinase produced by Bacillus licheniformis JP2 from Penen Hot Springs, North Sumatera. Precipitation of crude chitinase was performed with different levels of ammonium sulphate. Chitinase activity was determined colorimetrically by detecting the amount of N-acetylglucosamine (GlcNAc) released from colloidal chitin substrate with N-acetylglucosamine. Characterization of chitinase activity was measured at different pH, different temperature, Km and Vmax values. The optimum activity of chitinase was produced 4 days after incubation with chitinase activity of 0.0063 U/ml. Optimum chitinase activity was higher after 50% ammonium sulphate precipitation with activity of 0.0087 U/ml followed by decreasing in activity supernatant with activity of 0.0012 U/ml. Chitinase activity increased at pH 6 and at 60°C by 0.05066 U/ml with specific activity 3.8113 U/mg after dialysis. Km and Vmax values were 0.321 mg/ml and 71.429 µg, respectively.},
year = {2016}
}
TY - JOUR T1 - Characterization of Extraceluller Chitinase Produced by Bacillus licheniformis JP2 from Penen Hot Springs, North Sumatera AU - Jane Melita Keliat AU - Dwi Suryanto AU - Erman Munir Y1 - 2016/11/08 PY - 2016 N1 - https://doi.org/10.11648/j.fem.20160205.11 DO - 10.11648/j.fem.20160205.11 T2 - Frontiers in Environmental Microbiology JF - Frontiers in Environmental Microbiology JO - Frontiers in Environmental Microbiology SP - 24 EP - 27 PB - Science Publishing Group SN - 2469-8067 UR - https://doi.org/10.11648/j.fem.20160205.11 AB - The research was about characterization of extraceluller chitinase produced by Bacillus licheniformis JP2 from Penen Hot Springs, North Sumatera. Precipitation of crude chitinase was performed with different levels of ammonium sulphate. Chitinase activity was determined colorimetrically by detecting the amount of N-acetylglucosamine (GlcNAc) released from colloidal chitin substrate with N-acetylglucosamine. Characterization of chitinase activity was measured at different pH, different temperature, Km and Vmax values. The optimum activity of chitinase was produced 4 days after incubation with chitinase activity of 0.0063 U/ml. Optimum chitinase activity was higher after 50% ammonium sulphate precipitation with activity of 0.0087 U/ml followed by decreasing in activity supernatant with activity of 0.0012 U/ml. Chitinase activity increased at pH 6 and at 60°C by 0.05066 U/ml with specific activity 3.8113 U/mg after dialysis. Km and Vmax values were 0.321 mg/ml and 71.429 µg, respectively. VL - 2 IS - 5 ER -
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