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Analysis of Antibacterial Activity of Antibiotics to Anaerobes of Female Genital Tract in Vitro

Received: 14 February 2016     Accepted: 10 March 2016     Published: 24 March 2016
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Abstract

This study to investigate predominant anaerobic bacteria and their sensitivity spectrum to antibiotics in female patients with genital tract infections from the local and thus to provide scientific basis for the appropriate use of antibiotics. Anaerobic culture method was used to culture and isolate anaerobes in female patients with genital tract infections. Anaerobes were identified by using micro-biochemistry reacting technique and antibiotic-disc susceptibility test. Antibacterial activity of antibiotics to anaerobes was analyzed by means of Kirby-Bauer testing and broth micro-dilution method. The experiment results showed that 72 strains of obligate anaerobes were isolated from 103 samples, positive rate of 69.90%. The predominant anaerobic bacterial were Gram-negative non-spore bacteroides (28 strains, 38.89%) and anaerobic peptostreptococcus (18 strains, 25%). Sensitivity rate of anaerobic cocci, bacteroides and veillonella to metronidazole was 90.91%, 85.71% and 80% respectively. The drug resistance rates of the three isolated anaerobes were all higher than 60% to amikacin, erythromycin and clindamycin. Comparing the MIC50 and MIC90 values of metronidazole (MTZ), penicillin G (PCG) and lincomycin (LCM) on the isolated anaerobes, MTZ had the lowest MIC50 and MIC90 value. The study suggested that anaerobic infection has become a major pathogenic bacterium of infectious diseases in obstetrics and gynecology. Although MTZ has shown strong antibacterial activity in vitro, a few drug resistant strains appeared in the clinically isolated anaerobes and there is an upward trend of the MIC values of MTZ at different rates.

Published in European Journal of Preventive Medicine (Volume 4, Issue 2)
DOI 10.11648/j.ejpm.20160402.14
Page(s) 44-49
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Antibiotics, Anaerobic Infection, Female Genital Tract, Antibacterial Activity

References
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Cite This Article
  • APA Style

    Xuejun Zhan, Daze Xie, Xiangrong Shu, You Tang, Nanjin Zhou, et al. (2016). Analysis of Antibacterial Activity of Antibiotics to Anaerobes of Female Genital Tract in Vitro. European Journal of Preventive Medicine, 4(2), 44-49. https://doi.org/10.11648/j.ejpm.20160402.14

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    ACS Style

    Xuejun Zhan; Daze Xie; Xiangrong Shu; You Tang; Nanjin Zhou, et al. Analysis of Antibacterial Activity of Antibiotics to Anaerobes of Female Genital Tract in Vitro. Eur. J. Prev. Med. 2016, 4(2), 44-49. doi: 10.11648/j.ejpm.20160402.14

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    AMA Style

    Xuejun Zhan, Daze Xie, Xiangrong Shu, You Tang, Nanjin Zhou, et al. Analysis of Antibacterial Activity of Antibiotics to Anaerobes of Female Genital Tract in Vitro. Eur J Prev Med. 2016;4(2):44-49. doi: 10.11648/j.ejpm.20160402.14

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  • @article{10.11648/j.ejpm.20160402.14,
      author = {Xuejun Zhan and Daze Xie and Xiangrong Shu and You Tang and Nanjin Zhou and Ge Dai},
      title = {Analysis of Antibacterial Activity of Antibiotics to Anaerobes of Female Genital Tract in Vitro},
      journal = {European Journal of Preventive Medicine},
      volume = {4},
      number = {2},
      pages = {44-49},
      doi = {10.11648/j.ejpm.20160402.14},
      url = {https://doi.org/10.11648/j.ejpm.20160402.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejpm.20160402.14},
      abstract = {This study to investigate predominant anaerobic bacteria and their sensitivity spectrum to antibiotics in female patients with genital tract infections from the local and thus to provide scientific basis for the appropriate use of antibiotics. Anaerobic culture method was used to culture and isolate anaerobes in female patients with genital tract infections. Anaerobes were identified by using micro-biochemistry reacting technique and antibiotic-disc susceptibility test. Antibacterial activity of antibiotics to anaerobes was analyzed by means of Kirby-Bauer testing and broth micro-dilution method. The experiment results showed that 72 strains of obligate anaerobes were isolated from 103 samples, positive rate of 69.90%. The predominant anaerobic bacterial were Gram-negative non-spore bacteroides (28 strains, 38.89%) and anaerobic peptostreptococcus (18 strains, 25%). Sensitivity rate of anaerobic cocci, bacteroides and veillonella to metronidazole was 90.91%, 85.71% and 80% respectively. The drug resistance rates of the three isolated anaerobes were all higher than 60% to amikacin, erythromycin and clindamycin. Comparing the MIC50 and MIC90 values of metronidazole (MTZ), penicillin G (PCG) and lincomycin (LCM) on the isolated anaerobes, MTZ had the lowest MIC50 and MIC90 value. The study suggested that anaerobic infection has become a major pathogenic bacterium of infectious diseases in obstetrics and gynecology. Although MTZ has shown strong antibacterial activity in vitro, a few drug resistant strains appeared in the clinically isolated anaerobes and there is an upward trend of the MIC values of MTZ at different rates.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - Analysis of Antibacterial Activity of Antibiotics to Anaerobes of Female Genital Tract in Vitro
    AU  - Xuejun Zhan
    AU  - Daze Xie
    AU  - Xiangrong Shu
    AU  - You Tang
    AU  - Nanjin Zhou
    AU  - Ge Dai
    Y1  - 2016/03/24
    PY  - 2016
    N1  - https://doi.org/10.11648/j.ejpm.20160402.14
    DO  - 10.11648/j.ejpm.20160402.14
    T2  - European Journal of Preventive Medicine
    JF  - European Journal of Preventive Medicine
    JO  - European Journal of Preventive Medicine
    SP  - 44
    EP  - 49
    PB  - Science Publishing Group
    SN  - 2330-8230
    UR  - https://doi.org/10.11648/j.ejpm.20160402.14
    AB  - This study to investigate predominant anaerobic bacteria and their sensitivity spectrum to antibiotics in female patients with genital tract infections from the local and thus to provide scientific basis for the appropriate use of antibiotics. Anaerobic culture method was used to culture and isolate anaerobes in female patients with genital tract infections. Anaerobes were identified by using micro-biochemistry reacting technique and antibiotic-disc susceptibility test. Antibacterial activity of antibiotics to anaerobes was analyzed by means of Kirby-Bauer testing and broth micro-dilution method. The experiment results showed that 72 strains of obligate anaerobes were isolated from 103 samples, positive rate of 69.90%. The predominant anaerobic bacterial were Gram-negative non-spore bacteroides (28 strains, 38.89%) and anaerobic peptostreptococcus (18 strains, 25%). Sensitivity rate of anaerobic cocci, bacteroides and veillonella to metronidazole was 90.91%, 85.71% and 80% respectively. The drug resistance rates of the three isolated anaerobes were all higher than 60% to amikacin, erythromycin and clindamycin. Comparing the MIC50 and MIC90 values of metronidazole (MTZ), penicillin G (PCG) and lincomycin (LCM) on the isolated anaerobes, MTZ had the lowest MIC50 and MIC90 value. The study suggested that anaerobic infection has become a major pathogenic bacterium of infectious diseases in obstetrics and gynecology. Although MTZ has shown strong antibacterial activity in vitro, a few drug resistant strains appeared in the clinically isolated anaerobes and there is an upward trend of the MIC values of MTZ at different rates.
    VL  - 4
    IS  - 2
    ER  - 

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Author Information
  • Key Laboratory of Jiangxi Medical Immunity and Biotherapy, Jiangxi Academy of Medical Science, Nanchang, China

  • Key Laboratory of Jiangxi Medical Immunity and Biotherapy, Jiangxi Academy of Medical Science, Nanchang, China

  • Department of Cinical Laboratory, Jiangxi Maternal and Children’s Health Care Hospital, Nanchang, China

  • Department of Clinical Laboratory, Pingxiang Hospital of Nanchang University, Pingxiang, China

  • Key Laboratory of Jiangxi Medical Immunity and Biotherapy, Jiangxi Academy of Medical Science, Nanchang, China

  • Key Laboratory of Jiangxi Medical Immunity and Biotherapy, Jiangxi Academy of Medical Science, Nanchang, China

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