European Journal of Clinical and Biomedical Sciences

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Activity of Lysosomal Enzymes During Triple Combination Antihypertensive Treatment of Arterial Hypertension

Received: Apr. 13, 2023    Accepted: Jun. 21, 2023    Published: Sep. 13, 2023
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Abstract

Summary: The aim of the study has been to study the biochemical parameters of blood, the activity of lysosomal enzymes in models of arterial hypertension (AH) and assess the effectiveness of three-component antihypertensive treatment. Research materials and methods: The experiment has included 23 rabbits of Chinchilla breed weighing 2.5-3.0kg, which the AH model has been created in. Of these, 8 rabbits which have not received antihypertensive therapy (AHT) have made up the control group. After 4 weeks of model creation, AHT has been started in 15 rabbits of the main group within 1 month. Over the course of 8 weeks, systolic blood pressure (SBP) and diastolic blood pressure (DBP) have been measured in rabbits using a veterinary electronic tonometer. Along with this, the level of creatinine, urea, residual nitrogen in the blood plasma, the activity of the enzymes lactate dehydrogenase (LDH) and the MB fraction of creatine phosphokinase (CPK-MB) have been determined, ECG studies have been performed. All rabbits have been prescribed valsartan/amlodipine/hydrochlorthiazide (80/12.5/5mg and 160/25/10mg), choosing an individual dose depending on blood pressure once a day per os. Results: During the study, along with an increase in blood pressure, an increase in the level of creatinine, residual nitrogen and urine in the blood plasma has been revealed when creating a model of hypertension, which has been accompanied by an increase during the month. A decrease in the activity of the enzyme LDH and CPK-MB has been also noted. After the therapy hold for 1 month, positive dynamics have been found both in the level of blood pressure and indicators of biochemical analysis. Since creatinine concentration has decreased by 28.5%, residual nitrogen – by 36%, urea – by 40%, LDH activity has increased by 30%, CPK-MB by 40%. Conclusion: Thus, along with an increase in blood pressure and indicators of biochemical analysis, a decrease in the activity of lysosomal enzymes is noted in AP models. An increase in the activity of the enzymes LDH and CPK-MB during the three-component AHT shows that the components of this combination affect various parts of the cellular metabolism.

DOI 10.11648/j.ejcbs.20230904.11
Published in European Journal of Clinical and Biomedical Sciences ( Volume 9, Issue 4, August 2023 )
Page(s) 47-50
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Experimental Models, Arterial Hypertension, Lysosomal Enzymes, Fixed Drug Combination

References
[1] Drozdova G. A., Rumyantseva E. G., Mikheev M. S., Mustyatsa V. F. Clinical indicators and protein spectrum of Azova M. M, Blagonravov M. L, Frolov V. A. Apoptosis of cardiomyocytes in vasorenal arterial hypertension as a result of disturbance of the energy balance of cells // Kazan medical journal, 2013, volume 94, No. 1, p. 68-70.
[2] Sattar M., Yusof A., Gan E. Et. al Acute renal failure in 2K2C Goldblatt hypertensive rats during antihypertensive therapy: comparison of an angiotensin AT1 receptor antagonist and clonidine analogues // Journal of Autonomic Pharmacology, 2000, p. 297-304.
[3] Yamamoto E., Kataoka K., Dong Y. Et al. Calcium Channel Blockers, More than Diuretics, Enhance Vascular Protective Effects of Angiotensin Receptor Blockers in Salt-Loaded Hypertensive Rats // www.plosone.org June 2012, v 7, Issue 6, e. 391-62.
[4] Mubarashkina O. A., Somova M. N. Modern approaches to the use of triple combinations in the treatment of arterial hypertension // ConsiliumMedicum, 2017, №10, p. 39-42.
[5] Blagonravov M. L., Azova M. M., Frolov V. A. Biochemical study of apoptosis of myocardial cells in acute overload of the left ventricle in the experiment //Issues of biological, medical and pharmaceutical chemistryjournal, 2010, No. 8, p. 49–53.
[6] Goldblatt H., Kahn J. R. Experimental hypertension: constriction of the aorta at various levels // JAMA. — 1938. — Vol. 9. — P. 685.
[7] Drozdova G. A., Rumyantseva E. G., Mikheev M. S., Mustyatsa V. F. Clinical indicators and protein spectrum of blood in arterial renovascular hypertension in the experiment // Bulletin of Peoples Friendship University of Russia (VESTNIK RUDN), 2003, №2, p. 12-18.
[8] Mustyats V. F. Functional and structural features of the myocardium during the use of antihypertensive drugs in conditions of experimental arterial hypertension. Abstract of the dissertation for the degree of Doctor of Medical Sciences / Моscow, 2005, p. 12-15.
[9] Miesel A., Müller-Fielitz H., Jöhren O. Et. al Double blockade of angiotensin II (AT1) receptors and ACE does not improve weight gain and glucose homeostasis better than single-drug treatments in obese rats // British Journal of Pharmacology, 2012, 165, p. 2721–2735.
[10] Mills, Katherine T., Andrei Stefanescu, and Jiang He. "The global epidemiology of hypertension." Nature Reviews Nephrology 16.4 (2020): 223-237.
[11] Waisman G. Hipertensión arterial en el anciano [Arterial hypertension in the elderly]. Hipertens Riesgo Vasc. 2017 Apr-Jun; 34 (2): 61-64. Spanish. doi: 10.1016/j.hipert.2017.03.004. PMID: 28433228.
[12] Omboni S, Volpe M. Management of arterial hypertension with angiotensin receptor blockers: Current evidence and the role of olmesartan. Cardiovasc Ther. 2018 Dec; 36 (6): e12471. doi: 10.1111/1755-5922.12471. Epub 2018 Nov 13. PMID: 30358114; PMCID: PMC6587798.
[13] Brouwers, Sofie, et al. "Arterial hypertension." The Lancet (2021).
[14] Morozova, T. E., and I. Y. Yudina. "Triple combinations in the treatment of hypertension are a real way to improve blood pressure control." Consilium Medicum 19.1 (2017).
[15] Di Giosia P, Giorgini P, Stamerra CA, Petrarca M, Ferri C, Sahebkar A. Gender Differences in Epidemiology, Pathophysiology, and Treatment of Hypertension. Curr Atheroscler Rep. 2018 Feb 14; 20 (3): 13. doi: 10.1007/s11883-018-0716-z. PMID: 29445908.
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    Azayeva Nurana Rafail Gizi. (2023). Activity of Lysosomal Enzymes During Triple Combination Antihypertensive Treatment of Arterial Hypertension. European Journal of Clinical and Biomedical Sciences, 9(4), 47-50. https://doi.org/10.11648/j.ejcbs.20230904.11

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    Azayeva Nurana Rafail Gizi. Activity of Lysosomal Enzymes During Triple Combination Antihypertensive Treatment of Arterial Hypertension. Eur. J. Clin. Biomed. Sci. 2023, 9(4), 47-50. doi: 10.11648/j.ejcbs.20230904.11

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    AMA Style

    Azayeva Nurana Rafail Gizi. Activity of Lysosomal Enzymes During Triple Combination Antihypertensive Treatment of Arterial Hypertension. Eur J Clin Biomed Sci. 2023;9(4):47-50. doi: 10.11648/j.ejcbs.20230904.11

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  • @article{10.11648/j.ejcbs.20230904.11,
      author = {Azayeva Nurana Rafail Gizi},
      title = {Activity of Lysosomal Enzymes During Triple Combination Antihypertensive Treatment of Arterial Hypertension},
      journal = {European Journal of Clinical and Biomedical Sciences},
      volume = {9},
      number = {4},
      pages = {47-50},
      doi = {10.11648/j.ejcbs.20230904.11},
      url = {https://doi.org/10.11648/j.ejcbs.20230904.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ejcbs.20230904.11},
      abstract = {Summary: The aim of the study has been to study the biochemical parameters of blood, the activity of lysosomal enzymes in models of arterial hypertension (AH) and assess the effectiveness of three-component antihypertensive treatment. Research materials and methods: The experiment has included 23 rabbits of Chinchilla breed weighing 2.5-3.0kg, which the AH model has been created in. Of these, 8 rabbits which have not received antihypertensive therapy (AHT) have made up the control group. After 4 weeks of model creation, AHT has been started in 15 rabbits of the main group within 1 month. Over the course of 8 weeks, systolic blood pressure (SBP) and diastolic blood pressure (DBP) have been measured in rabbits using a veterinary electronic tonometer. Along with this, the level of creatinine, urea, residual nitrogen in the blood plasma, the activity of the enzymes lactate dehydrogenase (LDH) and the MB fraction of creatine phosphokinase (CPK-MB) have been determined, ECG studies have been performed. All rabbits have been prescribed valsartan/amlodipine/hydrochlorthiazide (80/12.5/5mg and 160/25/10mg), choosing an individual dose depending on blood pressure once a day per os. Results: During the study, along with an increase in blood pressure, an increase in the level of creatinine, residual nitrogen and urine in the blood plasma has been revealed when creating a model of hypertension, which has been accompanied by an increase during the month. A decrease in the activity of the enzyme LDH and CPK-MB has been also noted. After the therapy hold for 1 month, positive dynamics have been found both in the level of blood pressure and indicators of biochemical analysis. Since creatinine concentration has decreased by 28.5%, residual nitrogen – by 36%, urea – by 40%, LDH activity has increased by 30%, CPK-MB by 40%. Conclusion: Thus, along with an increase in blood pressure and indicators of biochemical analysis, a decrease in the activity of lysosomal enzymes is noted in AP models. An increase in the activity of the enzymes LDH and CPK-MB during the three-component AHT shows that the components of this combination affect various parts of the cellular metabolism.},
     year = {2023}
    }
    

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  • TY  - JOUR
    T1  - Activity of Lysosomal Enzymes During Triple Combination Antihypertensive Treatment of Arterial Hypertension
    AU  - Azayeva Nurana Rafail Gizi
    Y1  - 2023/09/13
    PY  - 2023
    N1  - https://doi.org/10.11648/j.ejcbs.20230904.11
    DO  - 10.11648/j.ejcbs.20230904.11
    T2  - European Journal of Clinical and Biomedical Sciences
    JF  - European Journal of Clinical and Biomedical Sciences
    JO  - European Journal of Clinical and Biomedical Sciences
    SP  - 47
    EP  - 50
    PB  - Science Publishing Group
    SN  - 2575-5005
    UR  - https://doi.org/10.11648/j.ejcbs.20230904.11
    AB  - Summary: The aim of the study has been to study the biochemical parameters of blood, the activity of lysosomal enzymes in models of arterial hypertension (AH) and assess the effectiveness of three-component antihypertensive treatment. Research materials and methods: The experiment has included 23 rabbits of Chinchilla breed weighing 2.5-3.0kg, which the AH model has been created in. Of these, 8 rabbits which have not received antihypertensive therapy (AHT) have made up the control group. After 4 weeks of model creation, AHT has been started in 15 rabbits of the main group within 1 month. Over the course of 8 weeks, systolic blood pressure (SBP) and diastolic blood pressure (DBP) have been measured in rabbits using a veterinary electronic tonometer. Along with this, the level of creatinine, urea, residual nitrogen in the blood plasma, the activity of the enzymes lactate dehydrogenase (LDH) and the MB fraction of creatine phosphokinase (CPK-MB) have been determined, ECG studies have been performed. All rabbits have been prescribed valsartan/amlodipine/hydrochlorthiazide (80/12.5/5mg and 160/25/10mg), choosing an individual dose depending on blood pressure once a day per os. Results: During the study, along with an increase in blood pressure, an increase in the level of creatinine, residual nitrogen and urine in the blood plasma has been revealed when creating a model of hypertension, which has been accompanied by an increase during the month. A decrease in the activity of the enzyme LDH and CPK-MB has been also noted. After the therapy hold for 1 month, positive dynamics have been found both in the level of blood pressure and indicators of biochemical analysis. Since creatinine concentration has decreased by 28.5%, residual nitrogen – by 36%, urea – by 40%, LDH activity has increased by 30%, CPK-MB by 40%. Conclusion: Thus, along with an increase in blood pressure and indicators of biochemical analysis, a decrease in the activity of lysosomal enzymes is noted in AP models. An increase in the activity of the enzymes LDH and CPK-MB during the three-component AHT shows that the components of this combination affect various parts of the cellular metabolism.
    VL  - 9
    IS  - 4
    ER  - 

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  • Scientific-Research Institute of Cardiology Names After Acad. J. Abdullayev, Baku, Azerbaijan

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