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Fosfomycin to Control Contamination in M. tuberculosis Culture in BACTEC MGIT 960 System

Received: 9 July 2018     Accepted: 16 August 2018     Published: 12 September 2018
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Abstract

The BACTEC MGIT 960 system is widely used for recovery of Mycobacterium tuberculosis, but high contamination rates compromise its diagnostic performance. Efforts to control contamination must balance elimination of contaminating organisms while preserving growth of tuberculous bacilli. Fosfomycin has activity against many bacteria responsible for culture contamination yet is inactive against Mycobacterium tuberculosis. Therefore, it holds potential as a selection agent for the culture of M. tuberculosis clinical samples with the MGIT system. In this study, we assess the ability of fosfomycin supplement to MGIT cultures of clinical sputum specimens. Sputum specimens collected at 62 diagnosis and treatment monitoring visits during a randomized controlled trial of high-dose rifampin (ClinicalTrials.gov NCT01408914) were split: one-half was treated as conventional and the other half was treated with 1mg of fosfomycin. The frequencies of contamination and of recovery of M. tuberculosis were compared using McNemar and Wilcoxon signed-rank test, respectively, with a=0.05. In the fosfomycin-treated samples, 2/62 (3.2%) yielded contaminated cultures while 12/62 (19.4%) of untreated cultures were contaminated (p<0.001, McNemar Test). Detection of M. tuberculosis was not significantly altered by the addition of fosfomycin (Time to positivity, p=0.576, Student’s T Test; log colony forming units), nor the Colony Forming Unit Counts (Wilcoxon signed-rank test p=0.671). These results suggest that the use of fosfomycin to control contamination does not affect the natural growth of MTB. Its use could result in a significant improvement in recovery of M. tuberculosis in MGIT.

Published in European Journal of Clinical and Biomedical Sciences (Volume 4, Issue 3)
DOI 10.11648/j.ejcbs.20180403.13
Page(s) 51-54
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Fosfomycin, Culture Contamination, Tuberculosis, BACTEC MGITTM 960

References
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    Roger Ivan Calderon Espinoza, Maria Belen Arriaga Gutierrez, Kattya Lopez Tamara, Nadia Nilda Barreda Ponce, Carole Diane Mitnick, et al. (2018). Fosfomycin to Control Contamination in M. tuberculosis Culture in BACTEC MGIT 960 System. European Journal of Clinical and Biomedical Sciences, 4(3), 51-54. https://doi.org/10.11648/j.ejcbs.20180403.13

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    ACS Style

    Roger Ivan Calderon Espinoza; Maria Belen Arriaga Gutierrez; Kattya Lopez Tamara; Nadia Nilda Barreda Ponce; Carole Diane Mitnick, et al. Fosfomycin to Control Contamination in M. tuberculosis Culture in BACTEC MGIT 960 System. Eur. J. Clin. Biomed. Sci. 2018, 4(3), 51-54. doi: 10.11648/j.ejcbs.20180403.13

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    AMA Style

    Roger Ivan Calderon Espinoza, Maria Belen Arriaga Gutierrez, Kattya Lopez Tamara, Nadia Nilda Barreda Ponce, Carole Diane Mitnick, et al. Fosfomycin to Control Contamination in M. tuberculosis Culture in BACTEC MGIT 960 System. Eur J Clin Biomed Sci. 2018;4(3):51-54. doi: 10.11648/j.ejcbs.20180403.13

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  • @article{10.11648/j.ejcbs.20180403.13,
      author = {Roger Ivan Calderon Espinoza and Maria Belen Arriaga Gutierrez and Kattya Lopez Tamara and Nadia Nilda Barreda Ponce and Carole Diane Mitnick and Geraint Rhys Davies and David John Coleman},
      title = {Fosfomycin to Control Contamination in M. tuberculosis Culture in BACTEC MGIT 960 System},
      journal = {European Journal of Clinical and Biomedical Sciences},
      volume = {4},
      number = {3},
      pages = {51-54},
      doi = {10.11648/j.ejcbs.20180403.13},
      url = {https://doi.org/10.11648/j.ejcbs.20180403.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejcbs.20180403.13},
      abstract = {The BACTEC MGIT 960 system is widely used for recovery of Mycobacterium tuberculosis, but high contamination rates compromise its diagnostic performance. Efforts to control contamination must balance elimination of contaminating organisms while preserving growth of tuberculous bacilli. Fosfomycin has activity against many bacteria responsible for culture contamination yet is inactive against Mycobacterium tuberculosis. Therefore, it holds potential as a selection agent for the culture of M. tuberculosis clinical samples with the MGIT system. In this study, we assess the ability of fosfomycin supplement to MGIT cultures of clinical sputum specimens. Sputum specimens collected at 62 diagnosis and treatment monitoring visits during a randomized controlled trial of high-dose rifampin (ClinicalTrials.gov NCT01408914) were split: one-half was treated as conventional and the other half was treated with 1mg of fosfomycin. The frequencies of contamination and of recovery of M. tuberculosis were compared using McNemar and Wilcoxon signed-rank test, respectively, with a=0.05. In the fosfomycin-treated samples, 2/62 (3.2%) yielded contaminated cultures while 12/62 (19.4%) of untreated cultures were contaminated (pM. tuberculosis was not significantly altered by the addition of fosfomycin (Time to positivity, p=0.576, Student’s T Test; log colony forming units), nor the Colony Forming Unit Counts (Wilcoxon signed-rank test p=0.671). These results suggest that the use of fosfomycin to control contamination does not affect the natural growth of MTB. Its use could result in a significant improvement in recovery of M. tuberculosis in MGIT.},
     year = {2018}
    }
    

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    T1  - Fosfomycin to Control Contamination in M. tuberculosis Culture in BACTEC MGIT 960 System
    AU  - Roger Ivan Calderon Espinoza
    AU  - Maria Belen Arriaga Gutierrez
    AU  - Kattya Lopez Tamara
    AU  - Nadia Nilda Barreda Ponce
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    AU  - Geraint Rhys Davies
    AU  - David John Coleman
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    T2  - European Journal of Clinical and Biomedical Sciences
    JF  - European Journal of Clinical and Biomedical Sciences
    JO  - European Journal of Clinical and Biomedical Sciences
    SP  - 51
    EP  - 54
    PB  - Science Publishing Group
    SN  - 2575-5005
    UR  - https://doi.org/10.11648/j.ejcbs.20180403.13
    AB  - The BACTEC MGIT 960 system is widely used for recovery of Mycobacterium tuberculosis, but high contamination rates compromise its diagnostic performance. Efforts to control contamination must balance elimination of contaminating organisms while preserving growth of tuberculous bacilli. Fosfomycin has activity against many bacteria responsible for culture contamination yet is inactive against Mycobacterium tuberculosis. Therefore, it holds potential as a selection agent for the culture of M. tuberculosis clinical samples with the MGIT system. In this study, we assess the ability of fosfomycin supplement to MGIT cultures of clinical sputum specimens. Sputum specimens collected at 62 diagnosis and treatment monitoring visits during a randomized controlled trial of high-dose rifampin (ClinicalTrials.gov NCT01408914) were split: one-half was treated as conventional and the other half was treated with 1mg of fosfomycin. The frequencies of contamination and of recovery of M. tuberculosis were compared using McNemar and Wilcoxon signed-rank test, respectively, with a=0.05. In the fosfomycin-treated samples, 2/62 (3.2%) yielded contaminated cultures while 12/62 (19.4%) of untreated cultures were contaminated (pM. tuberculosis was not significantly altered by the addition of fosfomycin (Time to positivity, p=0.576, Student’s T Test; log colony forming units), nor the Colony Forming Unit Counts (Wilcoxon signed-rank test p=0.671). These results suggest that the use of fosfomycin to control contamination does not affect the natural growth of MTB. Its use could result in a significant improvement in recovery of M. tuberculosis in MGIT.
    VL  - 4
    IS  - 3
    ER  - 

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Author Information
  • Socios En Salud Sucursal Peru, Lima, Peru

  • Faculdade de Medicina, Universidade Federal da Bahia, Bahia, Brazil

  • Socios En Salud Sucursal Peru, Lima, Peru

  • Socios En Salud Sucursal Peru, Lima, Peru

  • Department of Global Health and Social Medicine, Harvard Medical School, Boston, USA

  • Institutes of Infection and Global Health & Translational Medicine. University of Liverpool, Liverpool, United Kingdom

  • Socios En Salud Sucursal Peru, Lima, Peru

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