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Evaluation of Genome Size, Chromosome Number and Karyotype in Zygophyllum xanthoxylon

Received: 31 March 2021     Accepted: 14 April 2021     Published: 23 April 2021
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Abstract

Zygophyllum xanthoxylon, a super-xerophytic shrub in drylands, is widely used for afforestation in the arid and barren mountains of central Asia. Understanding the karyotype and genome size could provide basic information for genome sequencing of species. To date, few data on the DNA content and chromosomal characterization of Z. xanthoxylon have been reported. Here, we present both the karyotype analysis and genome size determination of Z. xanthoxylon based on the traditional pressing and flow cytometry methods. Chromosome counting showed that Z. xanthoxylon is diploid with a chromosome number of 22. Karyotype analysis revealed that the length of chromosomes ranges from 0.88 ± 0.08 μm to 2.36 ± 0.19 μm, the chromosomes are metacentric or submetacentric, and the karyotype formula is 2n = 2x = 22 = 18m + 4sm. Flow cytometry analysis estimated that the nuclear genome size of Z. xanthoxylon is 460 ± 7.05 Mbp. Interestingly, our results indicated the seedlings of Z. xanthoxylon exhibit endopolyploidy, which may confer better ecological adaptation. Collectively, the present study will provide an important cytological basis for the study of the origin, evolution and utilization of Z. xanthoxylon.

Published in Ecology and Evolutionary Biology (Volume 6, Issue 2)
DOI 10.11648/j.eeb.20210602.11
Page(s) 31-37
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Zygophyllum xanthoxylon, Chromosome Number, Karyotype, Flow Cytometry, Genomic Size, Endopolyploidy

References
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  • APA Style

    Yanbo Wu, Linjing Zhang, Yue Wang, Cui Liu, Shengdan Wu. (2021). Evaluation of Genome Size, Chromosome Number and Karyotype in Zygophyllum xanthoxylon. Ecology and Evolutionary Biology, 6(2), 31-37. https://doi.org/10.11648/j.eeb.20210602.11

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    ACS Style

    Yanbo Wu; Linjing Zhang; Yue Wang; Cui Liu; Shengdan Wu. Evaluation of Genome Size, Chromosome Number and Karyotype in Zygophyllum xanthoxylon. Ecol. Evol. Biol. 2021, 6(2), 31-37. doi: 10.11648/j.eeb.20210602.11

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    AMA Style

    Yanbo Wu, Linjing Zhang, Yue Wang, Cui Liu, Shengdan Wu. Evaluation of Genome Size, Chromosome Number and Karyotype in Zygophyllum xanthoxylon. Ecol Evol Biol. 2021;6(2):31-37. doi: 10.11648/j.eeb.20210602.11

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  • @article{10.11648/j.eeb.20210602.11,
      author = {Yanbo Wu and Linjing Zhang and Yue Wang and Cui Liu and Shengdan Wu},
      title = {Evaluation of Genome Size, Chromosome Number and Karyotype in Zygophyllum xanthoxylon},
      journal = {Ecology and Evolutionary Biology},
      volume = {6},
      number = {2},
      pages = {31-37},
      doi = {10.11648/j.eeb.20210602.11},
      url = {https://doi.org/10.11648/j.eeb.20210602.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.eeb.20210602.11},
      abstract = {Zygophyllum xanthoxylon, a super-xerophytic shrub in drylands, is widely used for afforestation in the arid and barren mountains of central Asia. Understanding the karyotype and genome size could provide basic information for genome sequencing of species. To date, few data on the DNA content and chromosomal characterization of Z. xanthoxylon have been reported. Here, we present both the karyotype analysis and genome size determination of Z. xanthoxylon based on the traditional pressing and flow cytometry methods. Chromosome counting showed that Z. xanthoxylon is diploid with a chromosome number of 22. Karyotype analysis revealed that the length of chromosomes ranges from 0.88 ± 0.08 μm to 2.36 ± 0.19 μm, the chromosomes are metacentric or submetacentric, and the karyotype formula is 2n = 2x = 22 = 18m + 4sm. Flow cytometry analysis estimated that the nuclear genome size of Z. xanthoxylon is 460 ± 7.05 Mbp. Interestingly, our results indicated the seedlings of Z. xanthoxylon exhibit endopolyploidy, which may confer better ecological adaptation. Collectively, the present study will provide an important cytological basis for the study of the origin, evolution and utilization of Z. xanthoxylon.},
     year = {2021}
    }
    

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  • TY  - JOUR
    T1  - Evaluation of Genome Size, Chromosome Number and Karyotype in Zygophyllum xanthoxylon
    AU  - Yanbo Wu
    AU  - Linjing Zhang
    AU  - Yue Wang
    AU  - Cui Liu
    AU  - Shengdan Wu
    Y1  - 2021/04/23
    PY  - 2021
    N1  - https://doi.org/10.11648/j.eeb.20210602.11
    DO  - 10.11648/j.eeb.20210602.11
    T2  - Ecology and Evolutionary Biology
    JF  - Ecology and Evolutionary Biology
    JO  - Ecology and Evolutionary Biology
    SP  - 31
    EP  - 37
    PB  - Science Publishing Group
    SN  - 2575-3762
    UR  - https://doi.org/10.11648/j.eeb.20210602.11
    AB  - Zygophyllum xanthoxylon, a super-xerophytic shrub in drylands, is widely used for afforestation in the arid and barren mountains of central Asia. Understanding the karyotype and genome size could provide basic information for genome sequencing of species. To date, few data on the DNA content and chromosomal characterization of Z. xanthoxylon have been reported. Here, we present both the karyotype analysis and genome size determination of Z. xanthoxylon based on the traditional pressing and flow cytometry methods. Chromosome counting showed that Z. xanthoxylon is diploid with a chromosome number of 22. Karyotype analysis revealed that the length of chromosomes ranges from 0.88 ± 0.08 μm to 2.36 ± 0.19 μm, the chromosomes are metacentric or submetacentric, and the karyotype formula is 2n = 2x = 22 = 18m + 4sm. Flow cytometry analysis estimated that the nuclear genome size of Z. xanthoxylon is 460 ± 7.05 Mbp. Interestingly, our results indicated the seedlings of Z. xanthoxylon exhibit endopolyploidy, which may confer better ecological adaptation. Collectively, the present study will provide an important cytological basis for the study of the origin, evolution and utilization of Z. xanthoxylon.
    VL  - 6
    IS  - 2
    ER  - 

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Author Information
  • College of Life Sciences, Shanxi Normal University, Linfen, China

  • College of Life Sciences, Shanxi Normal University, Linfen, China

  • College of Life Sciences, Shanxi Normal University, Linfen, China

  • College of Life Sciences, Shanxi Normal University, Linfen, China

  • State Key Laboratory of Grassland Agro-Ecosystem, Institute of Innovation Ecology, Lanzhou University, Lanzhou, China

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