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DAPK Gene Methylation Application in the Early Diagnosis of Nasopharyngeal Cancer

Received: 2 June 2023     Accepted: 21 June 2023     Published: 6 July 2023
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Abstract

Background: Nasopharyngeal cancer is a tumor of high malignancy originating from the epithelial tissues of the nasopharynx, which is often misdiagnosed and missed due to insidious lesions and inconspicuous early symptoms, and is a serious threat to human health. The development mechanism of nasopharyngeal cancer is still not well understood. Death-associated protein kinase (DAPK) is a calcium- and calmodulin- dependent serine and threonine protein kinase, which is involved in the apoptotic process induced by interferon-γ, tumor necrosis factor-α, Fas, etc. and has a pro-apoptotic function. The expression of DAPK in nasopharyngeal cancer tissues was related to the methylation status of CpG islands in the promoter region of its gene. Methylation of DAPK gene may be related to the occurrence and development of nasopharyngeal carcinoma. Objectives: The purpose of this article is to investigate the application value of DAPK gene methylation in the early diagnosis of nasopharyngeal cancer. Method: Methylation-specific PCR, pyrophosphate sequencing, real-time quantitative PCR and Western Blot test were used to detect the methylation of DAPK gene and its expression status in the blood of patients with nasopharyngeal cancer and chronic rhinopharyngitis. Result: There was no significant difference in the methylation rate and expression of DAPK gene in the blood of patients with nasopharyngeal cancer and chronic rhinopharyngitis. Conclusion: DAPK gene methylation cannot be used as an indicator for early diagnosis of nasopharyngeal cancer.

Published in Clinical Medicine Research (Volume 12, Issue 3)
DOI 10.11648/j.cmr.20231203.13
Page(s) 57-60
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

Nasopharyngeal Cancer, Methylation, Death-Associated Protein Kinase, Polymerase Chain Reaction

References
[1] Kwong J, Lo KW, Chow LS, To KF et al. Epigenetic silencing of cellular retinol-binding proteins in nasopharyngeal carcinoma. Neoplasia, 2005; 7 (1): 67-74.
[2] TAO Q, CHAN A T. Nasopharyngeal carcinoma: molecular pathogenesis and therapeutic developments. Expert Rev Mol Med. 2007 May 4; 9 (12): 1-24.
[3] Brock MV, Hooker CM, Ota-Machida E et al. DNA methylation markers and early recurrence in stage I lung cancer. N Engl J Med. 2008, 13; 358 (11): 1118-28.
[4] Deiss LP, Feinstein E, Berissi H, et al. Identification of a novel serine/threonine kinase and a novel 15-kD protein as potential mediators of the gamma interferon-induced cell death. Genes Dev, 1995, 9: 15230.
[5] Cohen O, Feinstein E, Kimchi A. DAP-kinase is a Ca2+ / calmodulin-dependent, cytoskeletal-associated protein kinase, with cell death-inducing functions that depend on its catalytic activity. EMBO J, 1997, 16: 9982-1008.
[6] Michie AM, McCaig AM, Nakagawa R, et al. Death-associated protein kinase (DAPK) and signal transduction: regulation in cancer. FEBS J 2010; 277: 74–80.
[7] Kwong J, Lo K, To K. Promoter hypermethylation of multiple genes in nasopharyngeal carcinoma promoter hypermethylation of multiple genes in. Clin Cancer Res 2002; 8: 131–7.
[8] Kong W J, Zhang S, GUO CK, et al. Effect of methylation-associated silencing of the deathassociated protein kinase gene on nasopharyngeal carcinoma, Anti-Cancer Drugs 2006, 17 (3): 251-259.
[9] Huang, Y; Wang, C; Li, K; Lin, Y. Death-associated protein kinase 1 suppresses hepatocellular carcinoma cell migration and invasion by upregulation of DEAD-box helicase 20, CANCER SCI. 2020, 111 (8): 2803-2813.
[10] Zhai, J; Yang, X; Zhang, Y; Wang, Q; Reduced expression levels of the death-associated protein kinase and E-cadherin are correlated with the development of esophageal squamous cell carcinoma, EXP THER MED. 2013, 5 (3): 972-976.
[11] Zhang, J; Shen, Z; Liu, H; Shu, W. Diagnostic potential of methylated DAPK in brushing samples of nasopharyngeal carcinoma. Cancer Manag Res. 2018; 10: 2953-2964.
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Cite This Article
  • APA Style

    Song Zhang, Jiahui Tang, Hongyan Yuan. (2023). DAPK Gene Methylation Application in the Early Diagnosis of Nasopharyngeal Cancer. Clinical Medicine Research, 12(3), 57-60. https://doi.org/10.11648/j.cmr.20231203.13

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    ACS Style

    Song Zhang; Jiahui Tang; Hongyan Yuan. DAPK Gene Methylation Application in the Early Diagnosis of Nasopharyngeal Cancer. Clin. Med. Res. 2023, 12(3), 57-60. doi: 10.11648/j.cmr.20231203.13

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    AMA Style

    Song Zhang, Jiahui Tang, Hongyan Yuan. DAPK Gene Methylation Application in the Early Diagnosis of Nasopharyngeal Cancer. Clin Med Res. 2023;12(3):57-60. doi: 10.11648/j.cmr.20231203.13

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  • @article{10.11648/j.cmr.20231203.13,
      author = {Song Zhang and Jiahui Tang and Hongyan Yuan},
      title = {DAPK Gene Methylation Application in the Early Diagnosis of Nasopharyngeal Cancer},
      journal = {Clinical Medicine Research},
      volume = {12},
      number = {3},
      pages = {57-60},
      doi = {10.11648/j.cmr.20231203.13},
      url = {https://doi.org/10.11648/j.cmr.20231203.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cmr.20231203.13},
      abstract = {Background: Nasopharyngeal cancer is a tumor of high malignancy originating from the epithelial tissues of the nasopharynx, which is often misdiagnosed and missed due to insidious lesions and inconspicuous early symptoms, and is a serious threat to human health. The development mechanism of nasopharyngeal cancer is still not well understood. Death-associated protein kinase (DAPK) is a calcium- and calmodulin- dependent serine and threonine protein kinase, which is involved in the apoptotic process induced by interferon-γ, tumor necrosis factor-α, Fas, etc. and has a pro-apoptotic function. The expression of DAPK in nasopharyngeal cancer tissues was related to the methylation status of CpG islands in the promoter region of its gene. Methylation of DAPK gene may be related to the occurrence and development of nasopharyngeal carcinoma. Objectives: The purpose of this article is to investigate the application value of DAPK gene methylation in the early diagnosis of nasopharyngeal cancer. Method: Methylation-specific PCR, pyrophosphate sequencing, real-time quantitative PCR and Western Blot test were used to detect the methylation of DAPK gene and its expression status in the blood of patients with nasopharyngeal cancer and chronic rhinopharyngitis. Result: There was no significant difference in the methylation rate and expression of DAPK gene in the blood of patients with nasopharyngeal cancer and chronic rhinopharyngitis. Conclusion: DAPK gene methylation cannot be used as an indicator for early diagnosis of nasopharyngeal cancer.},
     year = {2023}
    }
    

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  • TY  - JOUR
    T1  - DAPK Gene Methylation Application in the Early Diagnosis of Nasopharyngeal Cancer
    AU  - Song Zhang
    AU  - Jiahui Tang
    AU  - Hongyan Yuan
    Y1  - 2023/07/06
    PY  - 2023
    N1  - https://doi.org/10.11648/j.cmr.20231203.13
    DO  - 10.11648/j.cmr.20231203.13
    T2  - Clinical Medicine Research
    JF  - Clinical Medicine Research
    JO  - Clinical Medicine Research
    SP  - 57
    EP  - 60
    PB  - Science Publishing Group
    SN  - 2326-9057
    UR  - https://doi.org/10.11648/j.cmr.20231203.13
    AB  - Background: Nasopharyngeal cancer is a tumor of high malignancy originating from the epithelial tissues of the nasopharynx, which is often misdiagnosed and missed due to insidious lesions and inconspicuous early symptoms, and is a serious threat to human health. The development mechanism of nasopharyngeal cancer is still not well understood. Death-associated protein kinase (DAPK) is a calcium- and calmodulin- dependent serine and threonine protein kinase, which is involved in the apoptotic process induced by interferon-γ, tumor necrosis factor-α, Fas, etc. and has a pro-apoptotic function. The expression of DAPK in nasopharyngeal cancer tissues was related to the methylation status of CpG islands in the promoter region of its gene. Methylation of DAPK gene may be related to the occurrence and development of nasopharyngeal carcinoma. Objectives: The purpose of this article is to investigate the application value of DAPK gene methylation in the early diagnosis of nasopharyngeal cancer. Method: Methylation-specific PCR, pyrophosphate sequencing, real-time quantitative PCR and Western Blot test were used to detect the methylation of DAPK gene and its expression status in the blood of patients with nasopharyngeal cancer and chronic rhinopharyngitis. Result: There was no significant difference in the methylation rate and expression of DAPK gene in the blood of patients with nasopharyngeal cancer and chronic rhinopharyngitis. Conclusion: DAPK gene methylation cannot be used as an indicator for early diagnosis of nasopharyngeal cancer.
    VL  - 12
    IS  - 3
    ER  - 

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Author Information
  • Department of Otolaryngology, University of Chinese Academy of Science-Shenzhen Hospital, Shenzhen, China

  • Department of Otolaryngology, University of Chinese Academy of Science-Shenzhen Hospital, Shenzhen, China

  • Department of Otolaryngology, University of Chinese Academy of Science-Shenzhen Hospital, Shenzhen, China

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