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Identification of Animal Muscle Proteins by 2D-Electrophoresis and Time-of-Flight Mass Spectrometry

Received: 14 January 2017     Accepted: 25 January 2017     Published: 4 March 2017
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Abstract

A method for determining the quantitative content of meat proteins by two-dimensional electrophoresis (2D). The method is designed to quantify muscle protein in raw meat samples through proteomic technology complex comprising a two-dimensional polyacrylamide gel electrophoresis and densitometry obtained electrophoregrams computer.

Published in Chemical and Biomolecular Engineering (Volume 2, Issue 2)
DOI 10.11648/j.cbe.20170202.14
Page(s) 96-105
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Proteomics, Two-Dimensional Electrophoresis, Biomarkers, Mass Spectrometry

References
[1] Anderson N. L., Anderson N. G. 1998. Proteome and proteomics: new technologies, new concepts, and new words. Electrophoresis, 19: 1853-1861.
[2] Blum H., Beir H., Cross H. 1987. Improved silver staining of plant proteins, RNA and DNA inpolyacrylamide gels. Electrophoresis, 8: 126-129.
[3] Fairbanks G., Steck T. L., Wallach D. F. 1971. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry, 10: 2607-2617.
[4] Hoffmann K. 1997. Identification and determination of meat and foreign proteins by means of dodecylsulfat poliacrylamid gel electrophoresis. Ann. Nutr. Alim., 31: 207-215.
[5] O'Farrell P. H. 1975. High Resolution two-dimensional analysis of proteins. J. Biol. Chem., 250: 4007-4021.
[6] Kitchen C. M. R. 2009. Nonparametric vs parametric tests oflocation in biomedical research. American Journal of Ophthalmology, 147: 571-572.
[7] Govorun V. M., Moszkowski S. A., Tikhonova O. V. 2003. Comparative proteomic characterization of clinical isolates of cards. Biochemistry, 68: 52-58.
[8] Kovaleva M. A., Ivanov A. V., Kovalev L. I., Shishkin S. S., Hriapova E. E., Lisitsin A. B., Chernuha I. M., Vostrikova N. L. 2012. Proteomic technology in research of protein composition of cooked sausages. All about meat, 2: 35-42.
[9] Kovaleva M. A., Kovalev L. I., Shishkin S. S., Ivanov A. V., Vostrikova N. L., Chernuha I. M. 2013. Proteomic study of raw meat, cooked sausages and meat products of functional. Modern problems of science and education, 5: pp. 8-12.
[10] Chernuha I. M., Vostrikovа N. L. 2011. Coll. Materials of the 14th Intern. Scien. Conf. "Promising areas of research in the field of processing of meat raw materials and the creation of competitive foods" V. M. Gorbatov memory. Moscow, VNIIMP: 239-241.
[11] Lisitsyn A. B., Chernukha I. M., Ivankin A. N. 2013. Comparative study of fatty acid composition of meat material from various animal species. Scientific Journal of Animal Science, 2: 124–131.
[12] Neklyudov A. D., Ivankin A. N., Berdutina A. V. 2000. Properties and uses of protein hydrolysates. Applied Biochemistry and Microbiology, 36: 452-459.
Cite This Article
  • APA Style

    Natalia Leonid Vostrikova, Andrew Vladimir Kulikovskii, Irina Mihail Chernuha, Andrew Nikolay Ivankin. (2017). Identification of Animal Muscle Proteins by 2D-Electrophoresis and Time-of-Flight Mass Spectrometry. Chemical and Biomolecular Engineering, 2(2), 96-105. https://doi.org/10.11648/j.cbe.20170202.14

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    ACS Style

    Natalia Leonid Vostrikova; Andrew Vladimir Kulikovskii; Irina Mihail Chernuha; Andrew Nikolay Ivankin. Identification of Animal Muscle Proteins by 2D-Electrophoresis and Time-of-Flight Mass Spectrometry. Chem. Biomol. Eng. 2017, 2(2), 96-105. doi: 10.11648/j.cbe.20170202.14

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    AMA Style

    Natalia Leonid Vostrikova, Andrew Vladimir Kulikovskii, Irina Mihail Chernuha, Andrew Nikolay Ivankin. Identification of Animal Muscle Proteins by 2D-Electrophoresis and Time-of-Flight Mass Spectrometry. Chem Biomol Eng. 2017;2(2):96-105. doi: 10.11648/j.cbe.20170202.14

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  • @article{10.11648/j.cbe.20170202.14,
      author = {Natalia Leonid Vostrikova and Andrew Vladimir Kulikovskii and Irina Mihail Chernuha and Andrew Nikolay Ivankin},
      title = {Identification of Animal Muscle Proteins by 2D-Electrophoresis and Time-of-Flight Mass Spectrometry},
      journal = {Chemical and Biomolecular Engineering},
      volume = {2},
      number = {2},
      pages = {96-105},
      doi = {10.11648/j.cbe.20170202.14},
      url = {https://doi.org/10.11648/j.cbe.20170202.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cbe.20170202.14},
      abstract = {A method for determining the quantitative content of meat proteins by two-dimensional electrophoresis (2D). The method is designed to quantify muscle protein in raw meat samples through proteomic technology complex comprising a two-dimensional polyacrylamide gel electrophoresis and densitometry obtained electrophoregrams computer.},
     year = {2017}
    }
    

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    T1  - Identification of Animal Muscle Proteins by 2D-Electrophoresis and Time-of-Flight Mass Spectrometry
    AU  - Natalia Leonid Vostrikova
    AU  - Andrew Vladimir Kulikovskii
    AU  - Irina Mihail Chernuha
    AU  - Andrew Nikolay Ivankin
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    N1  - https://doi.org/10.11648/j.cbe.20170202.14
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    JF  - Chemical and Biomolecular Engineering
    JO  - Chemical and Biomolecular Engineering
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    UR  - https://doi.org/10.11648/j.cbe.20170202.14
    AB  - A method for determining the quantitative content of meat proteins by two-dimensional electrophoresis (2D). The method is designed to quantify muscle protein in raw meat samples through proteomic technology complex comprising a two-dimensional polyacrylamide gel electrophoresis and densitometry obtained electrophoregrams computer.
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    IS  - 2
    ER  - 

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Author Information
  • Department of Analytical Research, the V.M. Gorbatov All-Russian Meat Research Institute, Moscow, Russia

  • Department of Analytical Research, the V.M. Gorbatov All-Russian Meat Research Institute, Moscow, Russia

  • Department of Analytical Research, the V.M. Gorbatov All-Russian Meat Research Institute, Moscow, Russia

  • Department of Chemistry, Bauman Moscow State Technical University, Moscow, Russia

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