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In vitro Clonal Propagation of Korarima (Aframomum corrorima (Braun) P.C.M. Jansen) on Improved Varieties of Bonga1 and Benchmajji1

Received: 25 March 2022     Accepted: 20 April 2022     Published: 28 April 2022
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Abstract

Korarima (Aframomum corrorima (Braun) P.C.M. Jansen) is a diploid, 2n=48, monocious, perennial and aromatic herb species under family Zingiberaceae. It is a native plant preferred as an aromatic in regular Ethiopian serving dishes and locally choice in traditional medicine. However, commercial exploitation of this crop is hampered due to poor rate of seed germination and slow multiplication rate of the rhizomes along with other agronomic predicaments. Thus, the present study was aimed at in vitro micro propagations of two Aframomum corrorima cultivars of Bonga1 and Benchmajji1. Based on the results, better response was observed in rhizome bud explants with the highest mean number of micro shoots per explants, 4.33±0.33 and 4.67±0.33 for cv. Bonga1 and Benchmajji1 respectively, whereas 3.33±0.33 and 3.00±0.00 for cv. Bonga1 and Benchmajji1 respectively on the MS basal medium added BA 2.0mg/l and KN 0.5 mg/l. For multiplication, the highest mean number of shoots per explants (4.67±0.33) was obtained from the MS medium supplemented with combination of 3.0mg/l BA and 0.5 mg/l KN for cv. Bonga1, and the highest mean number of shoots per explants (4.00±0.00) has been found from the MS medium enhanced with 2.0mg/l BA from shoot tip explants. For rooting induction, the highest mean number of roots per explants (15.00±1.15) was obtained in the MS supplemented with 1.0mg/l NAA for cv. Bonga1, and the highest mean number of roots per explants (17.33±0.88) was observed in the MS supplemented with 1.5mg/l IBA and 2.0mg/l for genotype cv. Benchmajji1, but the highest root length was obtained in ex vitro rooting with sand and vermi-compost at ratio of 2:1 with 1mg/l IBA three times per a week.

Published in Cell Biology (Volume 10, Issue 1)
DOI 10.11648/j.cb.20221001.12
Page(s) 16-24
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

In vitro Clonal Propagation, Shoot Multiplication, Rooting Induction and Ex-vitro

References
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[2] Bhojwani S S and Dantu P K (2013) Plant Tissue Culture: An Introductory Text, Springer India.
[3] Dagmawit Ch and Endashaw B (2018) Genetic diversity analysis of cultivated Korarima (Aframomum corrorima (Braun) P.C.M. Jansen) populations from southwestern Ethiopia using inter simple sequence repeats (ISSR) marker J of Biol 25: 1-12.
[4] Eyob S (2009) Promotion of seed germination, subsequent seedling growth and in vitro propagation of korarima (Aframomum corrorima (Braun) P. C. M. Jansen) J. Med. Plants Res., 3 (9): 652-659.
[5] Eyob S, Appelgren M, Rohloff J, Tsegaye A, Messele G (2007). Chemical composition and physical properties of essential oils from fresh plant parts of korarima (Aframomum corrorima) cultivated in the highland of Southern Ethiopia. J. Essent. Oil Res. 19: 372-375.
[6] Eyob S, Martinsen K, Tsegaye A, Appelgren M, Skrede G (2008) Antioxidant and antimicrobial activities of extract and essential oil of korarima (Aframomum corrorima (Braun) P.C.M. Jansen). Afri. J. Biotechnol. 7 (15): 2585-2592.
[7] Fissiha G and Zemed M (2015) Analysis of Major Factors Affecting Production and Marketing of Korarima (Aframomum Corrorima (Braun) P.C.M. Jansen) in Ethiopia. Journal of Economics and Sustainable Development Vol. 6, No. 19.
[8] Girma H, Digafie T, Belay Y/B and Weyessa G, (2008) Spices research achievements Ethiopian Institute of Agricultural Research Revised edition, Addis Ababa, Ethiopia.
[9] Girma Hailemichael, Habtewold Kifelew and Haimanot Mitiku (2016) Spices Research Achievements, Challenges and Future Prospects in Ethiopia. Academic Research Journal of Agricultural Science and Research Vol. 4 (1), pp. 9-17.
[10] Israel P, Firew A and Samuel P M (2019) Socio-Economic and Environmental Values of Korarima (Aframomum corrorima (Braun) P.C.M. Jansen) IJAIR Vol. 7, 2319-1473.
[11] Jansen P C M (1981) Spices, condiments and medicinal plants in Ethiopia: their taxonomy and agricultural significance Agricultural Research Reports 906, Center for Agricultural Publishing and Documentation, Wageningen, Netherlands.
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[13] Leweye Getie (2017). In Vitro Propagation and Genetic Diversity Study of Korarima (Aframomum corrorima (Braun) P.C.M. Jansen) from Southern and Northwestern Ethiopia PhD dissertation, Addis Ababa University.
[14] Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.
[15] Rahiel H and Hailay G (2015) Effects of cytokinin types and their concentration on in vitro shoot induction and multiplication of korarima. International Journal of Genetics and Molecular Biology Vol. 7 (2), pp. 8-14.
[16] Simegn K, Sentayehu A, Melaku A (2016) Genetic Diversity studies on Yield and Its Related Traits in Korarima (Aframomum Corrorima (Braun) Jansen)) Germplasms; Journal of Biology, Agriculture and Healthcare; Vol. 6 (3).
[17] Teferi M (2016) In vitro propagation of korarima (Aframomum corrorima) from shoot explants. MSc Thesis. Addis Ababa University.
[18] Wannakrairoj S and Wondyifraw T (2013) In Vitro Chromosome Doubling in Korarima (Aframomum corrorima (Braun) P.C.M. Jansen) Using Colchicine and Oryzalin Asetsart J. (Nat. Sci.) 47: 684-694.
[19] Wondyifraw T and Wannakrairoj S (2004) Micropropagation of korarima (Aframomium corrorima (Braun) Jansen) Sci Asia 30: 1-7.
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    Berihu Mengs. (2022). In vitro Clonal Propagation of Korarima (Aframomum corrorima (Braun) P.C.M. Jansen) on Improved Varieties of Bonga1 and Benchmajji1. Cell Biology, 10(1), 16-24. https://doi.org/10.11648/j.cb.20221001.12

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    ACS Style

    Berihu Mengs. In vitro Clonal Propagation of Korarima (Aframomum corrorima (Braun) P.C.M. Jansen) on Improved Varieties of Bonga1 and Benchmajji1. Cell Biol. 2022, 10(1), 16-24. doi: 10.11648/j.cb.20221001.12

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    AMA Style

    Berihu Mengs. In vitro Clonal Propagation of Korarima (Aframomum corrorima (Braun) P.C.M. Jansen) on Improved Varieties of Bonga1 and Benchmajji1. Cell Biol. 2022;10(1):16-24. doi: 10.11648/j.cb.20221001.12

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  • @article{10.11648/j.cb.20221001.12,
      author = {Berihu Mengs},
      title = {In vitro Clonal Propagation of Korarima (Aframomum corrorima (Braun) P.C.M. Jansen) on Improved Varieties of Bonga1 and Benchmajji1},
      journal = {Cell Biology},
      volume = {10},
      number = {1},
      pages = {16-24},
      doi = {10.11648/j.cb.20221001.12},
      url = {https://doi.org/10.11648/j.cb.20221001.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cb.20221001.12},
      abstract = {Korarima (Aframomum corrorima (Braun) P.C.M. Jansen) is a diploid, 2n=48, monocious, perennial and aromatic herb species under family Zingiberaceae. It is a native plant preferred as an aromatic in regular Ethiopian serving dishes and locally choice in traditional medicine. However, commercial exploitation of this crop is hampered due to poor rate of seed germination and slow multiplication rate of the rhizomes along with other agronomic predicaments. Thus, the present study was aimed at in vitro micro propagations of two Aframomum corrorima cultivars of Bonga1 and Benchmajji1. Based on the results, better response was observed in rhizome bud explants with the highest mean number of micro shoots per explants, 4.33±0.33 and 4.67±0.33 for cv. Bonga1 and Benchmajji1 respectively, whereas 3.33±0.33 and 3.00±0.00 for cv. Bonga1 and Benchmajji1 respectively on the MS basal medium added BA 2.0mg/l and KN 0.5 mg/l. For multiplication, the highest mean number of shoots per explants (4.67±0.33) was obtained from the MS medium supplemented with combination of 3.0mg/l BA and 0.5 mg/l KN for cv. Bonga1, and the highest mean number of shoots per explants (4.00±0.00) has been found from the MS medium enhanced with 2.0mg/l BA from shoot tip explants. For rooting induction, the highest mean number of roots per explants (15.00±1.15) was obtained in the MS supplemented with 1.0mg/l NAA for cv. Bonga1, and the highest mean number of roots per explants (17.33±0.88) was observed in the MS supplemented with 1.5mg/l IBA and 2.0mg/l for genotype cv. Benchmajji1, but the highest root length was obtained in ex vitro rooting with sand and vermi-compost at ratio of 2:1 with 1mg/l IBA three times per a week.},
     year = {2022}
    }
    

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  • TY  - JOUR
    T1  - In vitro Clonal Propagation of Korarima (Aframomum corrorima (Braun) P.C.M. Jansen) on Improved Varieties of Bonga1 and Benchmajji1
    AU  - Berihu Mengs
    Y1  - 2022/04/28
    PY  - 2022
    N1  - https://doi.org/10.11648/j.cb.20221001.12
    DO  - 10.11648/j.cb.20221001.12
    T2  - Cell Biology
    JF  - Cell Biology
    JO  - Cell Biology
    SP  - 16
    EP  - 24
    PB  - Science Publishing Group
    SN  - 2330-0183
    UR  - https://doi.org/10.11648/j.cb.20221001.12
    AB  - Korarima (Aframomum corrorima (Braun) P.C.M. Jansen) is a diploid, 2n=48, monocious, perennial and aromatic herb species under family Zingiberaceae. It is a native plant preferred as an aromatic in regular Ethiopian serving dishes and locally choice in traditional medicine. However, commercial exploitation of this crop is hampered due to poor rate of seed germination and slow multiplication rate of the rhizomes along with other agronomic predicaments. Thus, the present study was aimed at in vitro micro propagations of two Aframomum corrorima cultivars of Bonga1 and Benchmajji1. Based on the results, better response was observed in rhizome bud explants with the highest mean number of micro shoots per explants, 4.33±0.33 and 4.67±0.33 for cv. Bonga1 and Benchmajji1 respectively, whereas 3.33±0.33 and 3.00±0.00 for cv. Bonga1 and Benchmajji1 respectively on the MS basal medium added BA 2.0mg/l and KN 0.5 mg/l. For multiplication, the highest mean number of shoots per explants (4.67±0.33) was obtained from the MS medium supplemented with combination of 3.0mg/l BA and 0.5 mg/l KN for cv. Bonga1, and the highest mean number of shoots per explants (4.00±0.00) has been found from the MS medium enhanced with 2.0mg/l BA from shoot tip explants. For rooting induction, the highest mean number of roots per explants (15.00±1.15) was obtained in the MS supplemented with 1.0mg/l NAA for cv. Bonga1, and the highest mean number of roots per explants (17.33±0.88) was observed in the MS supplemented with 1.5mg/l IBA and 2.0mg/l for genotype cv. Benchmajji1, but the highest root length was obtained in ex vitro rooting with sand and vermi-compost at ratio of 2:1 with 1mg/l IBA three times per a week.
    VL  - 10
    IS  - 1
    ER  - 

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Author Information
  • Plant Biotechnology Lab, Jimma Agricultural Research Center, Jimma, Ethiopia

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